4hxf - Revision history

OCA at 14:06, 13 March 2024

OCA — Wed, 13 Mar 2024 14:06:54 GMT

←Older revision		Revision as of 14:06, 13 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/O58323_PYRHO O58323_PYRHO]		[https://www.uniprot.org/uniprot/O58323_PYRHO O58323_PYRHO]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Oligopeptidases impose a size limitation on their substrates, the mechanism of which has long been in debate. Here we present the structure of a hexameric serine protease, an oligopeptidase from Pyrococcus horikoshii (PhAAP), revealing a complex, self-compartmentalized inner space, where substrates may access the monomer active sites passing through a double-gated "check-in" system: first passing through a pore on the hexamer surface, then turning to enter through an even smaller opening at the monomers' domain-interface. This substrate screening strategy is unique within the family. We found that among oligopeptidases a member of catalytic apparatus is positioned near an amylogenic beta-edge, which needs to be protected to prevent aggregation and found different strategies applied to such end. We propose that self-assembly within the family results in characteristically different substrate selection mechanisms coupled to different multimerization states.
-
-	A self-compartmentalizing hexamer serine protease from Pyrococcus horikoshii - substrate selection achieved through multimerization.,Menyhard DK, Kiss-Szeman A, Tichy-Racs E, Hornung B, Radi K, Szeltner Z, Domokos K, Szamosi I, Naray-Szabo G, Polgar L, Harmat V J Biol Chem. 2013 Apr 30. PMID:23632025<ref>PMID:23632025</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4hxf" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Acylaminoacyl peptidase 3D structures\|Acylaminoacyl peptidase 3D structures]]		*[[Acylaminoacyl peptidase 3D structures\|Acylaminoacyl peptidase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:12, 20 September 2023

OCA — Wed, 20 Sep 2023 15:12:25 GMT

←Older revision		Revision as of 15:12, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hxf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HXF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hxf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HXF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Y3A:N-[(BENZYLOXY)CARBONYL]GLYCYL-N-[(2S,3R)-4-CHLORO-3-HYDROXY-1-PHENYLBUTAN-2-YL]GLYCINAMIDE'>Y3A</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.601Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Y3A:N-[(BENZYLOXY)CARBONYL]GLYCYL-N-[(2S,3R)-4-CHLORO-3-HYDROXY-1-PHENYLBUTAN-2-YL]GLYCINAMIDE'>Y3A</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxf OCA], [https://pdbe.org/4hxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hxf RCSB], [https://www.ebi.ac.uk/pdbsum/4hxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxf OCA], [https://pdbe.org/4hxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hxf RCSB], [https://www.ebi.ac.uk/pdbsum/4hxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxf ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:24, 9 November 2022

OCA — Wed, 09 Nov 2022 08:24:15 GMT

←Older revision		Revision as of 08:24, 9 November 2022
Line 1:		Line 1:

	==Acylaminoacyl peptidase in complex with Z-Gly-Gly-Phe-chloromethyl ketone==		==Acylaminoacyl peptidase in complex with Z-Gly-Gly-Phe-chloromethyl ketone==
-	<StructureSection load='4hxf' size='340' side='right' caption='[[4hxf]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='4hxf' size='340' side='right'caption='[[4hxf]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hxf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pyrococcus_horikoshii~~ Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HXF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hxf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HXF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Y3A:N-[(BENZYLOXY)CARBONYL]GLYCYL-N-[(2S,3R)-4-CHLORO-3-HYDROXY-1-PHENYLBUTAN-2-YL]GLYCINAMIDE'>Y3A</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Y3A:N-[(BENZYLOXY)CARBONYL]GLYCYL-N-[(2S,3R)-4-CHLORO-3-HYDROXY-1-PHENYLBUTAN-2-YL]GLYCINAMIDE'>Y3A</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hxe\|4hxe]], [[4hxg\|4hxg]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxf OCA], [https://pdbe.org/4hxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hxf RCSB], [https://www.ebi.ac.uk/pdbsum/4hxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxf ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH0594 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acylaminoacyl-peptidase Acylaminoacyl-peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.1 3.4.19.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxf OCA], [~~http~~://pdbe.org/4hxf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hxf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hxf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxf ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O58323_PYRHO O58323_PYRHO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4hxf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4hxf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acylaminoacyl peptidase 3D structures\|Acylaminoacyl peptidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Acylaminoacyl-peptidase~~]]	+	[[Category: Large Structures]]
-	[[Category: Pyrococcus horikoshii]]	+	[[Category: Pyrococcus horikoshii OT3]]
-	[[Category: Domokos, K]]	+	[[Category: Domokos K]]
-	[[Category: Harmat, V]]	+	[[Category: Harmat V]]
-	[[Category: Hornung, B]]	+	[[Category: Hornung B]]
-	[[Category: Kiss-Szeman, A]]	+	[[Category: Kiss-Szeman A]]
-	[[Category: Menyhard~~, D K~~]]	+	[[Category: Menyhard DK]]
-	[[Category: Naray-Szabo, G]]	+	[[Category: Naray-Szabo G]]
-	[[Category: Polgar, L]]	+	[[Category: Polgar L]]
-	[[Category: Radi, K]]	+	[[Category: Radi K]]
-	[[Category: Szamosi, I]]	+	[[Category: Szamosi I]]
-	[[Category: Szeltner, Z]]	+	[[Category: Szeltner Z]]
-	[[Category: Tichy-Racs, E]]	+	[[Category: Tichy-Racs E]]
-	~~[[Category: Alpha/beta hyrdolase fold]]~~	+
-	~~[[Category: Beta-propeller]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Self-compartmentalization~~]]	+

OCA at 07:09, 18 October 2016

OCA — Tue, 18 Oct 2016 07:09:43 GMT

←Older revision		Revision as of 07:09, 18 October 2016
Line 3:		Line 3:
	<StructureSection load='4hxf' size='340' side='right' caption='[[4hxf]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4hxf' size='340' side='right' caption='[[4hxf]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hxf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrho Pyrho~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HXF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hxf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HXF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Y3A:N-[(BENZYLOXY)CARBONYL]GLYCYL-N-[(2S,3R)-4-CHLORO-3-HYDROXY-1-PHENYLBUTAN-2-YL]GLYCINAMIDE'>Y3A</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Y3A:N-[(BENZYLOXY)CARBONYL]GLYCYL-N-[(2S,3R)-4-CHLORO-3-HYDROXY-1-PHENYLBUTAN-2-YL]GLYCINAMIDE'>Y3A</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hxe\|4hxe]], [[4hxg\|4hxg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hxe\|4hxe]], [[4hxg\|4hxg]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH0594 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 ~~PYRHO~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH0594 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acylaminoacyl-peptidase Acylaminoacyl-peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.1 3.4.19.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acylaminoacyl-peptidase Acylaminoacyl-peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.1 3.4.19.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxf OCA], [http://pdbe.org/4hxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hxf RCSB], [http://www.ebi.ac.uk/pdbsum/4hxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxf OCA], [http://pdbe.org/4hxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hxf RCSB], [http://www.ebi.ac.uk/pdbsum/4hxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxf ProSAT]</span></td></tr>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Acylaminoacyl-peptidase]]		[[Category: Acylaminoacyl-peptidase]]
-	[[Category: ~~Pyrho~~]]	+	[[Category: Pyrococcus horikoshii]]
	[[Category: Domokos, K]]		[[Category: Domokos, K]]
	[[Category: Harmat, V]]		[[Category: Harmat, V]]

OCA at 08:14, 11 August 2016

OCA — Thu, 11 Aug 2016 08:14:23 GMT

←Older revision		Revision as of 08:14, 11 August 2016
Line 1:		Line 1:
		+
	==Acylaminoacyl peptidase in complex with Z-Gly-Gly-Phe-chloromethyl ketone==		==Acylaminoacyl peptidase in complex with Z-Gly-Gly-Phe-chloromethyl ketone==
	<StructureSection load='4hxf' size='340' side='right' caption='[[4hxf]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4hxf' size='340' side='right' caption='[[4hxf]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH0594 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 PYRHO])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH0594 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 PYRHO])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acylaminoacyl-peptidase Acylaminoacyl-peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.1 3.4.19.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acylaminoacyl-peptidase Acylaminoacyl-peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.1 3.4.19.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hxf RCSB], [http://www.ebi.ac.uk/pdbsum/4hxf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxf OCA], [http://pdbe.org/4hxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hxf RCSB], [http://www.ebi.ac.uk/pdbsum/4hxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hxf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:59, 20 January 2015

OCA — Tue, 20 Jan 2015 08:59:17 GMT

←Older revision		Revision as of 08:59, 20 January 2015
Line 23:		Line 23:
	[[Category: Acylaminoacyl-peptidase]]		[[Category: Acylaminoacyl-peptidase]]
	[[Category: Pyrho]]		[[Category: Pyrho]]
-	[[Category: Domokos, K.]]	+	[[Category: Domokos, K]]
-	[[Category: Harmat, V.]]	+	[[Category: Harmat, V]]
-	[[Category: Hornung, B.]]	+	[[Category: Hornung, B]]
-	[[Category: Kiss-Szeman, A.]]	+	[[Category: Kiss-Szeman, A]]
-	[[Category: Menyhard, D K.]]	+	[[Category: Menyhard, D K]]
-	[[Category: Naray-Szabo, G.]]	+	[[Category: Naray-Szabo, G]]
-	[[Category: Polgar, L.]]	+	[[Category: Polgar, L]]
-	[[Category: Radi, K.]]	+	[[Category: Radi, K]]
-	[[Category: Szamosi, I.]]	+	[[Category: Szamosi, I]]
-	[[Category: Szeltner, Z.]]	+	[[Category: Szeltner, Z]]
-	[[Category: Tichy-Racs, E.]]	+	[[Category: Tichy-Racs, E]]
	[[Category: Alpha/beta hyrdolase fold]]		[[Category: Alpha/beta hyrdolase fold]]
	[[Category: Beta-propeller]]		[[Category: Beta-propeller]]

OCA at 05:48, 8 October 2014

OCA — Wed, 08 Oct 2014 05:48:44 GMT

←Older revision		Revision as of 05:48, 8 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4hxf\| PDB=4hxf \| SCENE= }}~~	+	==Acylaminoacyl peptidase in complex with Z-Gly-Gly-Phe-chloromethyl ketone==
-	===Acylaminoacyl peptidase in complex with Z-Gly-Gly-Phe-chloromethyl ketone===	+	<StructureSection load='4hxf' size='340' side='right' caption='[[4hxf]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23632025}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hxf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrho Pyrho]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HXF FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Y3A:N-[(BENZYLOXY)CARBONYL]GLYCYL-N-[(2S,3R)-4-CHLORO-3-HYDROXY-1-PHENYLBUTAN-2-YL]GLYCINAMIDE'>Y3A</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hxe\|4hxe]], [[4hxg\|4hxg]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH0594 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 PYRHO])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acylaminoacyl-peptidase Acylaminoacyl-peptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.1 3.4.19.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hxf RCSB], [http://www.ebi.ac.uk/pdbsum/4hxf PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Oligopeptidases impose a size limitation on their substrates, the mechanism of which has long been in debate. Here we present the structure of a hexameric serine protease, an oligopeptidase from Pyrococcus horikoshii (PhAAP), revealing a complex, self-compartmentalized inner space, where substrates may access the monomer active sites passing through a double-gated "check-in" system: first passing through a pore on the hexamer surface, then turning to enter through an even smaller opening at the monomers' domain-interface. This substrate screening strategy is unique within the family. We found that among oligopeptidases a member of catalytic apparatus is positioned near an amylogenic beta-edge, which needs to be protected to prevent aggregation and found different strategies applied to such end. We propose that self-assembly within the family results in characteristically different substrate selection mechanisms coupled to different multimerization states.

-	~~==About this Structure==~~	+	A self-compartmentalizing hexamer serine protease from Pyrococcus horikoshii - substrate selection achieved through multimerization.,Menyhard DK, Kiss-Szeman A, Tichy-Racs E, Hornung B, Radi K, Szeltner Z, Domokos K, Szamosi I, Naray-Szabo G, Polgar L, Harmat V J Biol Chem. 2013 Apr 30. PMID:23632025<ref>PMID:23632025</ref>
-	~~[[4hxf]] is a 1 chain structure with sequence~~ from ~~[http://en.wikipedia.org/wiki/Pyrococcus_horikoshii_ot3~~ Pyrococcus horikoshii ~~ot3]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4HXF OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023632025</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Acylaminoacyl-peptidase]]		[[Category: Acylaminoacyl-peptidase]]
-	[[Category: ~~Pyrococcus horikoshii ot3~~]]	+	[[Category: Pyrho]]
	[[Category: Domokos, K.]]		[[Category: Domokos, K.]]
	[[Category: Harmat, V.]]		[[Category: Harmat, V.]]

OCA at 14:27, 3 July 2013

OCA — Wed, 03 Jul 2013 14:27:30 GMT

←Older revision		Revision as of 14:27, 3 July 2013
Line 4:		Line 4:

	==About this Structure==		==About this Structure==
-	[[4hxf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA].	+	[[4hxf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii_ot3 Pyrococcus horikoshii ot3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:023632025</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:023632025</ref><references group="xtra"/><references/>
	[[Category: Acylaminoacyl-peptidase]]		[[Category: Acylaminoacyl-peptidase]]
		+	[[Category: Pyrococcus horikoshii ot3]]
	[[Category: Domokos, K.]]		[[Category: Domokos, K.]]
	[[Category: Harmat, V.]]		[[Category: Harmat, V.]]

OCA at 11:06, 3 July 2013

OCA — Wed, 03 Jul 2013 11:06:18 GMT

←Older revision		Revision as of 11:06, 3 July 2013
Line 4:		Line 4:

	==About this Structure==		==About this Structure==
-	[[4hxf]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii_ot3 Pyrococcus horikoshii ot3]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA].	+	[[4hxf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023632025</ref><references group="xtra"/><references/>
	[[Category: Acylaminoacyl-peptidase]]		[[Category: Acylaminoacyl-peptidase]]
-	[[Category: Pyrococcus horikoshii ot3]]
	[[Category: Domokos, K.]]		[[Category: Domokos, K.]]
	[[Category: Harmat, V.]]		[[Category: Harmat, V.]]

OCA at 06:26, 16 May 2013

OCA — Thu, 16 May 2013 06:26:52 GMT

←Older revision		Revision as of 06:26, 16 May 2013
Line 1:		Line 1:
	{{STRUCTURE_4hxf\| PDB=4hxf \| SCENE= }}		{{STRUCTURE_4hxf\| PDB=4hxf \| SCENE= }}
	===Acylaminoacyl peptidase in complex with Z-Gly-Gly-Phe-chloromethyl ketone===		===Acylaminoacyl peptidase in complex with Z-Gly-Gly-Phe-chloromethyl ketone===
-	{{~~ABSTRACT_PUBMED_19303951~~}}	+	{{ABSTRACT_PUBMED_23632025}}

	==About this Structure==		==About this Structure==