4hxy - Revision history

OCA at 09:15, 15 November 2023

OCA — Wed, 15 Nov 2023 09:15:45 GMT

←Older revision		Revision as of 09:15, 15 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hxy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._HK803 Streptomyces sp. HK803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HXY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hxy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._HK803 Streptomyces sp. HK803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HXY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACA:6-AMINOHEXANOIC+ACID'>ACA</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.68Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACA:6-AMINOHEXANOIC+ACID'>ACA</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxy OCA], [https://pdbe.org/4hxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hxy RCSB], [https://www.ebi.ac.uk/pdbsum/4hxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxy OCA], [https://pdbe.org/4hxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hxy RCSB], [https://www.ebi.ac.uk/pdbsum/4hxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxy ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:25, 9 November 2022

OCA — Wed, 09 Nov 2022 08:25:20 GMT

←Older revision		Revision as of 08:25, 9 November 2022
Line 1:		Line 1:

	==PlmKR1-Ketoreductase from the first module of phoslactomycin biosynthesis in Streptomyces sp. HK803==		==PlmKR1-Ketoreductase from the first module of phoslactomycin biosynthesis in Streptomyces sp. HK803==
-	<StructureSection load='4hxy' size='340' side='right' caption='[[4hxy]], [[Resolution\|resolution]] 1.68Å' scene=''>	+	<StructureSection load='4hxy' size='340' side='right'caption='[[4hxy]], [[Resolution\|resolution]] 1.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hxy]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_sp.~~_hk803~~ Streptomyces sp. ~~hk803~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HXY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hxy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._HK803 Streptomyces sp. HK803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HXY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACA:6-AMINOHEXANOIC+ACID'>ACA</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACA:6-AMINOHEXANOIC+ACID'>ACA</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plm1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=244967 Streptomyces sp. HK803])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxy OCA], [https://pdbe.org/4hxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hxy RCSB], [https://www.ebi.ac.uk/pdbsum/4hxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxy ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxy OCA], [~~http~~://pdbe.org/4hxy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hxy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hxy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxy ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6V1M8_9ACTN Q6V1M8_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces sp. hk803~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Smith, J L~~]]	+	[[Category: Streptomyces sp. HK803]]
-	[[Category: ~~Whicher, J R~~]]	+	[[Category: Smith JL]]
-	[[Category: ~~Ketoreductase]]~~	+	[[Category: Whicher JR]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Short chain dehydrogenase/reductase~~]]	+

OCA at 13:55, 4 August 2016

OCA — Thu, 04 Aug 2016 13:55:46 GMT

←Older revision		Revision as of 13:55, 4 August 2016
Line 1:		Line 1:
		+
	==PlmKR1-Ketoreductase from the first module of phoslactomycin biosynthesis in Streptomyces sp. HK803==		==PlmKR1-Ketoreductase from the first module of phoslactomycin biosynthesis in Streptomyces sp. HK803==
	<StructureSection load='4hxy' size='340' side='right' caption='[[4hxy]], [[Resolution\|resolution]] 1.68Å' scene=''>		<StructureSection load='4hxy' size='340' side='right' caption='[[4hxy]], [[Resolution\|resolution]] 1.68Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACA:6-AMINOHEXANOIC+ACID'>ACA</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACA:6-AMINOHEXANOIC+ACID'>ACA</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plm1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=244967 Streptomyces sp. HK803])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plm1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=244967 Streptomyces sp. HK803])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hxy RCSB], [http://www.ebi.ac.uk/pdbsum/4hxy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxy OCA], [http://pdbe.org/4hxy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hxy RCSB], [http://www.ebi.ac.uk/pdbsum/4hxy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hxy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:42, 21 December 2014

OCA — Sun, 21 Dec 2014 12:42:10 GMT

←Older revision		Revision as of 12:42, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4hxy\| PDB=4hxy \| SCENE= }}~~	+	==PlmKR1-Ketoreductase from the first module of phoslactomycin biosynthesis in Streptomyces sp. HK803==
-	===PlmKR1-Ketoreductase from the first module of phoslactomycin biosynthesis in Streptomyces sp. HK803===	+	<StructureSection load='4hxy' size='340' side='right' caption='[[4hxy]], [[Resolution\|resolution]] 1.68Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23790488}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hxy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._hk803 Streptomyces sp. hk803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HXY FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACA:6-AMINOHEXANOIC+ACID'>ACA</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plm1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=244967 Streptomyces sp. HK803])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hxy RCSB], [http://www.ebi.ac.uk/pdbsum/4hxy PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The formation of an activated cis-3-cyclohexylpropenoic acid by Plm1, the first extension module of the phoslactomycin polyketide synthase, is proposed to occur through an L-3-hydroxyacyl-intermediate as a result of ketoreduction by an A-type ketoreductase (KR). Here, we demonstrate that the KR domain of Plm1 (PlmKR1) catalyzes the formation of an L-3-hydroxyacyl product. The crystal structure of PlmKR1 revealed a well-ordered active site with a nearby Trp residue characteristic of A-type KRs. Structural comparison of PlmKR1 with B-type KRs that produce D-3-hydroxyacyl intermediates revealed significant differences. The active site of cofactor-bound A-type KRs is in a catalysis-ready state, whereas cofactor-bound B-type KRs are in a precatalytic state. Furthermore, the closed lid loop in substrate-bound A-type KRs restricts active site access from all but one direction, which is proposed to control the stereochemistry of ketoreduction.

-	~~==About this Structure==~~	+	Structural and Stereochemical Analysis of a Modular Polyketide Synthase Ketoreductase Domain Required for the Generation of a cis-Alkene.,Bonnett SA, Whicher JR, Papireddy K, Florova G, Smith JL, Reynolds KA Chem Biol. 2013 Jun 20;20(6):772-83. doi: 10.1016/j.chembiol.2013.04.014. PMID:23790488<ref>PMID:23790488</ref>
-	~~[[4hxy]] is~~ a ~~2 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Streptomyces_sp~~.~~_hk803 Streptomyces sp~~. ~~hk803]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4HXY OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023790488</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces sp. hk803]]		[[Category: Streptomyces sp. hk803]]
-	[[Category: Smith, J L.]]	+	[[Category: Smith, J L]]
-	[[Category: Whicher, J R.]]	+	[[Category: Whicher, J R]]
	[[Category: Ketoreductase]]		[[Category: Ketoreductase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: Short chain dehydrogenase/reductase]]		[[Category: Short chain dehydrogenase/reductase]]

OCA at 15:36, 10 July 2013

OCA — Wed, 10 Jul 2013 15:36:43 GMT

←Older revision		Revision as of 15:36, 10 July 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4hxy\| PDB=4hxy \| SCENE= }}
		+	===PlmKR1-Ketoreductase from the first module of phoslactomycin biosynthesis in Streptomyces sp. HK803===
		+	{{ABSTRACT_PUBMED_23790488}}

-	~~The entry~~ 4hxy is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4hxy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._hk803 Streptomyces sp. hk803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXY OCA].

-	~~Authors~~: ~~Whicher, J~~.~~R.,~~ Smith, J.L.	+	==Reference==
-		+	<ref group="xtra">PMID:023790488</ref><references group="xtra"/><references/>
-	~~Description~~: ~~PlmKR1-~~Ketoreductase ~~from the first module of phoslactomycin biosynthesis in Streptomyces sp. HK803~~	+	[[Category: Streptomyces sp. hk803]]
		+	[[Category: Smith, J L.]]
		+	[[Category: Whicher, J R.]]
		+	[[Category: Ketoreductase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Short chain dehydrogenase/reductase]]

OCA: Protected "4hxy" [edit=sysop:move=sysop]

OCA — Wed, 21 Nov 2012 09:09:25 GMT

Protected "4hxy" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:09, 21 November 2012

OCA: New page: '''Unreleased structure''' The entry 4hxy is ON HOLD until Paper Publication Authors: Whicher, J.R., Smith, J.L. Description: PlmKR1-Ketoreductase from the first module of phoslactomyc...

OCA — Wed, 21 Nov 2012 09:09:24 GMT

New page: '''Unreleased structure''' The entry 4hxy is ON HOLD until Paper Publication Authors: Whicher, J.R., Smith, J.L. Description: PlmKR1-Ketoreductase from the first module of phoslactomyc...

New page

'''Unreleased structure'''

The entry 4hxy is ON HOLD until Paper Publication

Authors: Whicher, J.R., Smith, J.L.

Description: PlmKR1-Ketoreductase from the first module of phoslactomycin biosynthesis in Streptomyces sp. HK803