4hzb - Revision history

OCA at 08:29, 9 November 2022

OCA — Wed, 09 Nov 2022 08:29:15 GMT

←Older revision		Revision as of 08:29, 9 November 2022
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	==Crystal structure of the type VI SeMet effector-immunity complex Tae3-Tai3 from Ralstonia pickettii==		==Crystal structure of the type VI SeMet effector-immunity complex Tae3-Tai3 from Ralstonia pickettii==
-	<StructureSection load='4hzb' size='340' side='right' caption='[[4hzb]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='4hzb' size='340' side='right'caption='[[4hzb]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hzb]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ralp1 Ralp1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HZB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hzb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_pickettii_12D Ralstonia pickettii 12D]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HZB FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hz9\|4hz9]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzb OCA], [https://pdbe.org/4hzb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hzb RCSB], [https://www.ebi.ac.uk/pdbsum/4hzb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hzb ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rpic12D_3261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=428406 RALP1]), Rpic12D_3260 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=428406 RALP1])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzb OCA], [~~http~~://pdbe.org/4hzb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hzb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hzb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hzb ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C6BHF2_RALP1 C6BHF2_RALP1]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ralp1~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Dong, C~~]]	+	[[Category: Ralstonia pickettii 12D]]
-	[[Category: Dong~~, Y H~~]]	+	[[Category: Dong C]]
-	[[Category: ~~Gao, Z Q~~]]	+	[[Category: Dong YH]]
-	[[Category: ~~Zhang, H~~]]	+	[[Category: Gao ZQ]]
-	[[Category: ~~Alpha+beta]]~~	+	[[Category: Zhang H]]
-	~~[[Category: Endopeptidase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Periplasmic space]]~~	+
-	~~[[Category: Protein-protein complex~~]]	+

OCA at 22:49, 5 August 2016

OCA — Fri, 05 Aug 2016 22:49:35 GMT

←Older revision		Revision as of 22:49, 5 August 2016
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		+
	==Crystal structure of the type VI SeMet effector-immunity complex Tae3-Tai3 from Ralstonia pickettii==		==Crystal structure of the type VI SeMet effector-immunity complex Tae3-Tai3 from Ralstonia pickettii==
	<StructureSection load='4hzb' size='340' side='right' caption='[[4hzb]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4hzb' size='340' side='right' caption='[[4hzb]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hzb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Ralstonia_pickettii_12d Ralstonia pickettii 12d~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HZB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hzb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ralp1 Ralp1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HZB FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hz9\|4hz9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hz9\|4hz9]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rpic12D_3261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=428406 ~~Ralstonia pickettii 12D~~]), Rpic12D_3260 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=428406 ~~Ralstonia pickettii 12D~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rpic12D_3261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=428406 RALP1]), Rpic12D_3260 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=428406 RALP1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hzb RCSB], [http://www.ebi.ac.uk/pdbsum/4hzb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzb OCA], [http://pdbe.org/4hzb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hzb RCSB], [http://www.ebi.ac.uk/pdbsum/4hzb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hzb ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hzb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ralstonia pickettii 12d~~]]	+	[[Category: Ralp1]]
	[[Category: Dong, C]]		[[Category: Dong, C]]
	[[Category: Dong, Y H]]		[[Category: Dong, Y H]]

OCA at 12:39, 21 December 2014

OCA — Sun, 21 Dec 2014 12:39:50 GMT

←Older revision		Revision as of 12:39, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4hzb\| PDB=4hzb \| SCENE= }}~~	+	==Crystal structure of the type VI SeMet effector-immunity complex Tae3-Tai3 from Ralstonia pickettii==
-	===Crystal structure of the type VI SeMet effector-immunity complex Tae3-Tai3 from Ralstonia pickettii===	+	<StructureSection load='4hzb' size='340' side='right' caption='[[4hzb]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23730712}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hzb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ralstonia_pickettii_12d Ralstonia pickettii 12d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HZB FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hz9\|4hz9]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rpic12D_3261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=428406 Ralstonia pickettii 12D]), Rpic12D_3260 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=428406 Ralstonia pickettii 12D])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hzb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hzb RCSB], [http://www.ebi.ac.uk/pdbsum/4hzb PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The recently described T6SS (type VI secretion system) acts as a needle that punctures the membrane of the target cells to deliver effector proteins. Type VI amidase effectors can be classified into four divergent families (Tae1-Tae4). These effectors are secreted into the periplasmic space of neighbouring cells via the T6SS and subsequently rupture peptidoglycan. However, the donor cells are protected from damage because of the presence of their cognate immunity proteins [Tai1 (type VI amidase immunity 1)-Tai4]. In the present paper, we describe the structure of Tae3 in complex with Tai3. The Tae3-Tai3 complex exists as a stable heterohexamer, which is composed of two Tae3 molecules and two Tai3 homodimers (Tae3-Tai34-Tae3). Tae3 shares a common NlpC/P60 fold, which consists of N-terminal and C-terminal subdomains. Structural analysis indicates that two unique loops around the catalytic cleft adopt a closed conformation, resulting in a narrow and extended groove involved in the binding of the substrate. The inhibition of Tae3 is attributed to the insertion of the Omega-loop (loop of alpha3-alpha4) of Tai3 into the catalytic groove. Furthermore, a cell viability assay confirmed that a conserved motif (Gln-Asp-Xaa) in Tai3 members may play a key role in the inhibition process. Taken together, the present study has revealed a novel inhibition mechanism and provides insights into the role played by T6SS in interspecific competition.

-	~~==About this Structure==~~	+	Structural insights into the inhibition of type VI effector Tae3 by its immunity protein Tai3.,Dong C, Zhang H, Gao ZQ, Wang WJ, She Z, Liu GF, Shen YQ, Su XD, Dong YH Biochem J. 2013 Aug 15;454(1):59-68. doi: 10.1042/BJ20130193. PMID:23730712<ref>PMID:23730712</ref>
-	~~[[4hzb]] is a 6 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Ralstonia_pickettii_12d Ralstonia pickettii 12d]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4HZB OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023730712</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Ralstonia pickettii 12d]]		[[Category: Ralstonia pickettii 12d]]
-	[[Category: Dong, C.]]	+	[[Category: Dong, C]]
-	[[Category: Dong, Y H.]]	+	[[Category: Dong, Y H]]
-	[[Category: Gao, Z Q.]]	+	[[Category: Gao, Z Q]]
-	[[Category: Zhang, H.]]	+	[[Category: Zhang, H]]
	[[Category: Alpha+beta]]		[[Category: Alpha+beta]]
	[[Category: Endopeptidase]]		[[Category: Endopeptidase]]

OCA at 08:28, 21 August 2013

OCA — Wed, 21 Aug 2013 08:28:14 GMT

←Older revision		Revision as of 08:28, 21 August 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4hzb\| PDB=4hzb \| SCENE= }}
		+	===Crystal structure of the type VI SeMet effector-immunity complex Tae3-Tai3 from Ralstonia pickettii===
		+	{{ABSTRACT_PUBMED_23730712}}

-	~~The entry~~ 4hzb is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4hzb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ralstonia_pickettii_12d Ralstonia pickettii 12d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZB OCA].

-	~~Authors~~: Dong, C., ~~Zhang,~~ H., Gao, Z.Q., ~~Dong, Y.~~H.	+	==Reference==
-		+	<ref group="xtra">PMID:023730712</ref><references group="xtra"/><references/>
-	~~Description~~: ~~The crystal structure of RP1 in~~ complex ~~with RP2~~	+	[[Category: Ralstonia pickettii 12d]]
		+	[[Category: Dong, C.]]
		+	[[Category: Dong, Y H.]]
		+	[[Category: Gao, Z Q.]]
		+	[[Category: Zhang, H.]]
		+	[[Category: Alpha+beta]]
		+	[[Category: Endopeptidase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Periplasmic space]]
		+	[[Category: Protein-protein complex]]

OCA at 10:30, 5 December 2012

OCA — Wed, 05 Dec 2012 10:30:44 GMT

←Older revision		Revision as of 10:30, 5 December 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hzb is ON HOLD	+	The entry 4hzb is ON HOLD until Paper Publication

-	Authors: Dong, C, Zhang, H, Gao, Z.Q, Dong, Y.H~~, Program for the Characterization of Secreted Effector Proteins (PCSEP)~~	+	Authors: Dong, C., Zhang, H., Gao, Z.Q., Dong, Y.H.

	Description: The crystal structure of RP1 in complex with RP2		Description: The crystal structure of RP1 in complex with RP2

OCA: Protected "4hzb" [edit=sysop:move=sysop]

OCA — Wed, 21 Nov 2012 09:11:00 GMT

Protected "4hzb" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:11, 21 November 2012

OCA: New page: '''Unreleased structure''' The entry 4hzb is ON HOLD Authors: Dong, C, Zhang, H, Gao, Z.Q, Dong, Y.H, Program for the Characterization of Secreted Effector Proteins (PCSEP) Description...

OCA — Wed, 21 Nov 2012 09:10:59 GMT

New page: '''Unreleased structure''' The entry 4hzb is ON HOLD Authors: Dong, C, Zhang, H, Gao, Z.Q, Dong, Y.H, Program for the Characterization of Secreted Effector Proteins (PCSEP) Description...

New page

'''Unreleased structure'''

The entry 4hzb is ON HOLD

Authors: Dong, C, Zhang, H, Gao, Z.Q, Dong, Y.H, Program for the Characterization of Secreted Effector Proteins (PCSEP)

Description: The crystal structure of RP1 in complex with RP2