4i2z - Revision history

OCA at 08:37, 9 November 2022

OCA — Wed, 09 Nov 2022 08:37:38 GMT

←Older revision		Revision as of 08:37, 9 November 2022
Line 1:		Line 1:

	==Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp90 peptide==		==Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp90 peptide==
-	<StructureSection load='4i2z' size='340' side='right' caption='[[4i2z]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='4i2z' size='340' side='right'caption='[[4i2z]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4i2z]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Caeel Caeel~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I2Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4I2Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4i2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I2Z FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4i2w\|4i2w]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i2z OCA], [https://pdbe.org/4i2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i2z RCSB], [https://www.ebi.ac.uk/pdbsum/4i2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i2z ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">unc-45, CELE_F30H5.1, F30H5.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i2z OCA], [~~http~~://pdbe.org/4i2z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4i2z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4i2z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4i2z ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~HSP90_CAEEL HSP90_CAEEL~~]] Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Required to stabilize the daf-11/transmembrane guanylyl cyclases or another signal transduction component that regulates cGMP levels. Participates in the control of cell cycle progression at the prophase/metaphase transition in oocyte development by ensuring the activity of wee-1.3 kinase, which negatively regulates cdk-1 through its phosphorylation.<ref>PMID:10790386</ref> <ref>PMID:16466390</ref>	+	[https://www.uniprot.org/uniprot/G5EG62_CAEEL G5EG62_CAEEL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Caeel~~]]	+	[[Category: Caenorhabditis elegans]]
-	[[Category: ~~Clausen, T~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Gazda, L~~]]	+	[[Category: Clausen T]]
-	[[Category: ~~Hellerschmied, D~~]]	+	[[Category: Gazda L]]
-	[[Category: ~~Chaperone]]~~	+	[[Category: Hellerschmied D]]
-	~~[[Category: Chaperone-protein binding complex]]~~	+
-	~~[[Category: Hsp70 and hsp90 co-chaperone]]~~	+
-	~~[[Category: Myofilament formation]]~~	+
-	~~[[Category: Myosin folding]]~~	+
-	~~[[Category: Protein filament]]~~	+
-	~~[[Category: Tpr-peptide interaction]]~~	+
-	~~[[Category: Ucs domain containing protein~~]]	+

OCA at 20:24, 4 August 2016

OCA — Thu, 04 Aug 2016 20:24:52 GMT

←Older revision		Revision as of 20:24, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp90 peptide==		==Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp90 peptide==
	<StructureSection load='4i2z' size='340' side='right' caption='[[4i2z]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='4i2z' size='340' side='right' caption='[[4i2z]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4i2z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Caenorhabditis_elegans Caenorhabditis elegans~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I2Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I2Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4i2z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I2Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I2Z FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4i2w\|4i2w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4i2w\|4i2w]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">unc-45, CELE_F30H5.1, F30H5.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 ~~Caenorhabditis elegans~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">unc-45, CELE_F30H5.1, F30H5.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i2z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4i2z RCSB], [http://www.ebi.ac.uk/pdbsum/4i2z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i2z OCA], [http://pdbe.org/4i2z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4i2z RCSB], [http://www.ebi.ac.uk/pdbsum/4i2z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4i2z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4i2z" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Caenorhabditis elegans~~]]	+	[[Category: Caeel]]
	[[Category: Clausen, T]]		[[Category: Clausen, T]]
	[[Category: Gazda, L]]		[[Category: Gazda, L]]

OCA at 12:50, 25 December 2014

OCA — Thu, 25 Dec 2014 12:50:48 GMT

←Older revision		Revision as of 12:50, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i2z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4i2z RCSB], [http://www.ebi.ac.uk/pdbsum/4i2z PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i2z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4i2z RCSB], [http://www.ebi.ac.uk/pdbsum/4i2z PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HSP90_CAEEL HSP90_CAEEL]] Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Required to stabilize the daf-11/transmembrane guanylyl cyclases or another signal transduction component that regulates cGMP levels. Participates in the control of cell cycle progression at the prophase/metaphase transition in oocyte development by ensuring the activity of wee-1.3 kinase, which negatively regulates cdk-1 through its phosphorylation.<ref>PMID:10790386</ref> <ref>PMID:16466390</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:30, 18 December 2014

OCA — Thu, 18 Dec 2014 08:30:13 GMT

←Older revision		Revision as of 08:30, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4i2z\| PDB=4i2z \| SCENE= }}~~	+	==Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp90 peptide==
-	===Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp90 peptide===	+	<StructureSection load='4i2z' size='340' side='right' caption='[[4i2z]], [[Resolution\|resolution]] 2.90Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23332754}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4i2z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I2Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4I2Z FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4i2w\|4i2w]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">unc-45, CELE_F30H5.1, F30H5.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i2z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4i2z RCSB], [http://www.ebi.ac.uk/pdbsum/4i2z PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The UCS (UNC-45/CRO1/She4) chaperones play an evolutionarily conserved role in promoting myosin-dependent processes, including cytokinesis, endocytosis, RNA transport, and muscle development. To investigate the protein machinery orchestrating myosin folding and assembly, we performed a comprehensive analysis of Caenorhabditis elegans UNC-45. Our structural and biochemical data demonstrate that UNC-45 forms linear protein chains that offer multiple binding sites for cooperating chaperones and client proteins. Accordingly, Hsp70 and Hsp90, which bind to the TPR domain of UNC-45, could act in concert and with defined periodicity on captured myosin molecules. In vivo analyses reveal the elongated canyon of the UCS domain as a myosin-binding site and show that multimeric UNC-45 chains support organization of sarcomeric repeats. In fact, expression of transgenes blocking UNC-45 chain formation induces dominant-negative defects in the sarcomere structure and function of wild-type worms. Together, these findings uncover a filament assembly factor that directly couples myosin folding with myofilament formation.

-	~~==About this Structure==~~	+	The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans.,Gazda L, Pokrzywa W, Hellerschmied D, Lowe T, Forne I, Mueller-Planitz F, Hoppe T, Clausen T Cell. 2013 Jan 17;152(1-2):183-95. doi: 10.1016/j.cell.2012.12.025. PMID:23332754<ref>PMID:23332754</ref>
-	~~[[4i2z]]~~ is a 2 ~~chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki~~/~~Caenorhabditis_elegans Caenorhabditis elegans]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id~~=~~4I2Z OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Caenorhabditis elegans]]		[[Category: Caenorhabditis elegans]]
-	[[Category: Clausen, T.]]	+	[[Category: Clausen, T]]
-	[[Category: Gazda, L.]]	+	[[Category: Gazda, L]]
-	[[Category: Hellerschmied, D.]]	+	[[Category: Hellerschmied, D]]
	[[Category: Chaperone]]		[[Category: Chaperone]]
	[[Category: Chaperone-protein binding complex]]		[[Category: Chaperone-protein binding complex]]

OCA at 10:22, 13 March 2013

OCA — Wed, 13 Mar 2013 10:22:14 GMT

←Older revision		Revision as of 10:22, 13 March 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4i2z\| PDB=4i2z \| SCENE= }}
		+	===Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp90 peptide===
		+	{{ABSTRACT_PUBMED_23332754}}

-	~~The entry~~ 4i2z is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4i2z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I2Z OCA].
-	~~Authors~~: Clausen, T., Gazda, L., Hellerschmied, D.	+	[[Category: Caenorhabditis elegans]]
-		+	[[Category: Clausen, T.]]
-	~~Description~~: ~~Crystal structure of the myosin chaperone UNC~~-~~45 from C.elegans in~~ complex ~~with a Hsp90~~ peptide	+	[[Category: Gazda, L.]]
		+	[[Category: Hellerschmied, D.]]
		+	[[Category: Chaperone]]
		+	[[Category: Chaperone-protein binding complex]]
		+	[[Category: Hsp70 and hsp90 co-chaperone]]
		+	[[Category: Myofilament formation]]
		+	[[Category: Myosin folding]]
		+	[[Category: Protein filament]]
		+	[[Category: Tpr-peptide interaction]]
		+	[[Category: Ucs domain containing protein]]

OCA at 11:03, 19 December 2012

OCA — Wed, 19 Dec 2012 11:03:02 GMT

←Older revision		Revision as of 11:03, 19 December 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4i2z is ON HOLD	+	The entry 4i2z is ON HOLD until Paper Publication

	Authors: Clausen, T., Gazda, L., Hellerschmied, D.		Authors: Clausen, T., Gazda, L., Hellerschmied, D.

	Description: Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp90 peptide		Description: Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp90 peptide

OCA: Protected "4i2z" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 10:32:24 GMT

Protected "4i2z" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:32, 5 December 2012

OCA: New page: '''Unreleased structure''' The entry 4i2z is ON HOLD Authors: Clausen, T., Gazda, L., Hellerschmied, D. Description: Crystal structure of the myosin chaperone UNC-45 from C.elegans in ...

OCA — Wed, 05 Dec 2012 10:32:23 GMT

New page: '''Unreleased structure''' The entry 4i2z is ON HOLD Authors: Clausen, T., Gazda, L., Hellerschmied, D. Description: Crystal structure of the myosin chaperone UNC-45 from C.elegans in ...

New page

'''Unreleased structure'''

The entry 4i2z is ON HOLD

Authors: Clausen, T., Gazda, L., Hellerschmied, D.

Description: Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp90 peptide