4ihd - Revision history

OCA at 15:23, 20 September 2023

OCA — Wed, 20 Sep 2023 15:23:03 GMT

←Older revision		Revision as of 15:23, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ihd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IHD FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ihd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IHD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihd OCA], [https://pdbe.org/4ihd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ihd RCSB], [https://www.ebi.ac.uk/pdbsum/4ihd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihd OCA], [https://pdbe.org/4ihd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ihd RCSB], [https://www.ebi.ac.uk/pdbsum/4ihd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihd ProSAT]</span></td></tr>
	</table>		</table>

OCA at 20:45, 16 November 2022

OCA — Wed, 16 Nov 2022 20:45:11 GMT

←Older revision		Revision as of 20:45, 16 November 2022
Line 3:		Line 3:
	<StructureSection load='4ihd' size='340' side='right'caption='[[4ihd]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='4ihd' size='340' side='right'caption='[[4ihd]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ihd]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"streptomyces_cattleya"_kahan_et_al._1979 "streptomyces~~ cattleya~~" kahan et al. 1979~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4IHD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ihd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IHD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s3u\|3s3u]], [[3tm1\|3tm1]], [[3tm2\|3tm2]], [[4ihe\|4ihe]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihd OCA], [https://pdbe.org/4ihd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ihd RCSB], [https://www.ebi.ac.uk/pdbsum/4ihd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihd ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ThnT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 "Streptomyces cattleya" Kahan et al. 1979])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantetheine_hydrolase Pantetheine hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.92 3.5.1.92] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihd OCA], [~~http~~://pdbe.org/4ihd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ihd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ihd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihd ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q83XN4_STRCT Q83XN4_STRCT]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces cattleya kahan et al. 1979]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pantetheine hydrolase~~]]	+	[[Category: Streptomyces cattleya]]
-	[[Category: Buller~~, A R~~]]	+	[[Category: Buller AR]]
-	[[Category: Schildbach~~, J F~~]]	+	[[Category: Schildbach JF]]
-	[[Category: Townsend~~, C A]]~~	+	[[Category: Townsend CA]]
-	~~[[Category: Autoproteolysis]]~~	+
-	~~[[Category: Clan pe]]~~	+
-	~~[[Category: Dom-fold]]~~	+
-	~~[[Category: Ethylmercury derivatization of c282]]~~	+
-	~~[[Category: Family p1]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Thienamycin biosynthesis~~]]	+

OCA at 07:54, 27 March 2019

OCA — Wed, 27 Mar 2019 07:54:23 GMT

←Older revision		Revision as of 07:54, 27 March 2019
Line 1:		Line 1:

	==Crystal Structure of Uncleaved ThnT T282C, derivatized at the active site with EtHg==		==Crystal Structure of Uncleaved ThnT T282C, derivatized at the active site with EtHg==
-	<StructureSection load='4ihd' size='340' side='right' caption='[[4ihd]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='4ihd' size='340' side='right'caption='[[4ihd]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ihd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IHD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ihd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_cattleya"_kahan_et_al._1979 "streptomyces cattleya" kahan et al. 1979]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IHD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s3u\|3s3u]], [[3tm1\|3tm1]], [[3tm2\|3tm2]], [[4ihe\|4ihe]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s3u\|3s3u]], [[3tm1\|3tm1]], [[3tm2\|3tm2]], [[4ihe\|4ihe]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ThnT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 "Streptomyces cattleya" Kahan et al. 1979])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantetheine_hydrolase Pantetheine hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.92 3.5.1.92] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantetheine_hydrolase Pantetheine hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.92 3.5.1.92] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihd OCA], [http://pdbe.org/4ihd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ihd RCSB], [http://www.ebi.ac.uk/pdbsum/4ihd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihd OCA], [http://pdbe.org/4ihd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ihd RCSB], [http://www.ebi.ac.uk/pdbsum/4ihd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihd ProSAT]</span></td></tr>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Streptomyces cattleya kahan et al. 1979]]
		+	[[Category: Large Structures]]
	[[Category: Pantetheine hydrolase]]		[[Category: Pantetheine hydrolase]]
	[[Category: Buller, A R]]		[[Category: Buller, A R]]

OCA at 08:35, 11 August 2016

OCA — Thu, 11 Aug 2016 08:35:17 GMT

←Older revision		Revision as of 08:35, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Uncleaved ThnT T282C, derivatized at the active site with EtHg==		==Crystal Structure of Uncleaved ThnT T282C, derivatized at the active site with EtHg==
	<StructureSection load='4ihd' size='340' side='right' caption='[[4ihd]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='4ihd' size='340' side='right' caption='[[4ihd]], [[Resolution\|resolution]] 1.65Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s3u\|3s3u]], [[3tm1\|3tm1]], [[3tm2\|3tm2]], [[4ihe\|4ihe]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s3u\|3s3u]], [[3tm1\|3tm1]], [[3tm2\|3tm2]], [[4ihe\|4ihe]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantetheine_hydrolase Pantetheine hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.92 3.5.1.92] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantetheine_hydrolase Pantetheine hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.92 3.5.1.92] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ihd RCSB], [http://www.ebi.ac.uk/pdbsum/4ihd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihd OCA], [http://pdbe.org/4ihd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ihd RCSB], [http://www.ebi.ac.uk/pdbsum/4ihd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihd ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ihd" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:41, 5 January 2015

OCA — Mon, 05 Jan 2015 14:41:55 GMT

←Older revision		Revision as of 14:41, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ihd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IHD FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ihd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IHD FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s3u\|3s3u]], [[3tm1\|3tm1]], [[3tm2\|3tm2]], [[4ihe\|4ihe]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s3u\|3s3u]], [[3tm1\|3tm1]], [[3tm2\|3tm2]], [[4ihe\|4ihe]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantetheine_hydrolase Pantetheine hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.92 3.5.1.92] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantetheine_hydrolase Pantetheine hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.92 3.5.1.92] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ihd RCSB], [http://www.ebi.ac.uk/pdbsum/4ihd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ihd RCSB], [http://www.ebi.ac.uk/pdbsum/4ihd PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Pantetheine hydrolase]]		[[Category: Pantetheine hydrolase]]
-	[[Category: Buller, A R.]]	+	[[Category: Buller, A R]]
-	[[Category: Schildbach, J F.]]	+	[[Category: Schildbach, J F]]
-	[[Category: Townsend, C A.]]	+	[[Category: Townsend, C A]]
	[[Category: Autoproteolysis]]		[[Category: Autoproteolysis]]
	[[Category: Clan pe]]		[[Category: Clan pe]]
Line 30:		Line 30:
	[[Category: Family p1]]		[[Category: Family p1]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
-	[[Category: Pantetheine hydrolase]]
	[[Category: Thienamycin biosynthesis]]		[[Category: Thienamycin biosynthesis]]

OCA at 09:00, 13 August 2014

OCA — Wed, 13 Aug 2014 09:00:51 GMT

←Older revision		Revision as of 09:00, 13 August 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal Structure of Uncleaved ThnT T282C, derivatized at the active site with EtHg==
		+	<StructureSection load='4ihd' size='340' side='right' caption='[[4ihd]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ihd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IHD FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s3u\|3s3u]], [[3tm1\|3tm1]], [[3tm2\|3tm2]], [[4ihe\|4ihe]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantetheine_hydrolase Pantetheine hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.92 3.5.1.92] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ihd RCSB], [http://www.ebi.ac.uk/pdbsum/4ihd PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In the past decade, there have been major achievements in understanding the relationship between enzyme catalysis and protein structural plasticity. In autoprocessing systems, however, there is a sparsity of direct evidence of the role of conformational dynamics, which are complicated by their intrinsic chemical reactivity. ThnT is an autoproteolytically activated enzyme involved in the biosynthesis of the beta-lactam antibiotic thienamycin. Conservative mutation of ThnT results in multiple conformational states that can be observed via X-ray crystallography, establishing ThnT as a representative and revealing system for studing how conformational dynamics control autoactivation at a molecular level. Removal of the nucleophile by mutation to Ala disrupts the population of a reactive state and causes widespread structural changes from a conformation that promotes autoproteolysis to one associated with substrate catalysis. Finer probing of the active site polysterism was achieved by EtHg derivatization of the nucleophile, which indicates the active site and a neighboring loop have coupled dynamics. Disruption of these interactions by mutagenesis precludes the ability to observe a reactive state through X-ray crystallography, and application of this insight to other autoproteolytically activated enzymes offers an explanation for the widespread crystallization of inactive states. We suggest that the N --> O(S) acyl shift in cis-autoproteolysis might occur through a si-face attack, thereby unifying the fundamental chemistry of these enzymes through a common mechanism.

-	~~The entry 4ihd is ON HOLD until Paper Publication~~	+	Exploring the Role of Conformational Heterogeneity in cis-Autoproteolytic Activation of ThnT.,Buller AR, Freeman MF, Schildbach JF, Townsend CA Biochemistry. 2014 Jul 8;53(26):4273-81. doi: 10.1021/bi500385d. Epub 2014 Jun, 26. PMID:24933323<ref>PMID:24933323</ref>

-	~~Authors~~: Buller, A.R., Schildbach, J.F., Townsend, C.A.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal Structure~~ of ~~Uncleaved ThnT T282C, derivatized at the active site with EtHg~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Pantetheine hydrolase]]
		+	[[Category: Buller, A R.]]
		+	[[Category: Schildbach, J F.]]
		+	[[Category: Townsend, C A.]]
		+	[[Category: Autoproteolysis]]
		+	[[Category: Clan pe]]
		+	[[Category: Dom-fold]]
		+	[[Category: Ethylmercury derivatization of c282]]
		+	[[Category: Family p1]]
		+	[[Category: Hydrolase]]
		+	[[Category: Pantetheine hydrolase]]
		+	[[Category: Thienamycin biosynthesis]]

OCA at 08:49, 8 January 2014

OCA — Wed, 08 Jan 2014 08:49:42 GMT

←Older revision		Revision as of 08:49, 8 January 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ihd is ON HOLD	+	The entry 4ihd is ON HOLD until Paper Publication

	Authors: Buller, A.R., Schildbach, J.F., Townsend, C.A.		Authors: Buller, A.R., Schildbach, J.F., Townsend, C.A.

	Description: Crystal Structure of Uncleaved ThnT T282C, derivatized at the active site with EtHg		Description: Crystal Structure of Uncleaved ThnT T282C, derivatized at the active site with EtHg

OCA: Protected "4ihd" [edit=sysop:move=sysop]

OCA — Wed, 02 Jan 2013 08:58:31 GMT

Protected "4ihd" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:58, 2 January 2013

OCA: New page: '''Unreleased structure''' The entry 4ihd is ON HOLD Authors: Buller, A.R., Schildbach, J.F., Townsend, C.A. Description: Crystal Structure of Uncleaved ThnT T282C, derivatized at the ...

OCA — Wed, 02 Jan 2013 08:58:29 GMT

New page: '''Unreleased structure''' The entry 4ihd is ON HOLD Authors: Buller, A.R., Schildbach, J.F., Townsend, C.A. Description: Crystal Structure of Uncleaved ThnT T282C, derivatized at the ...

New page

'''Unreleased structure'''

The entry 4ihd is ON HOLD

Authors: Buller, A.R., Schildbach, J.F., Townsend, C.A.

Description: Crystal Structure of Uncleaved ThnT T282C, derivatized at the active site with EtHg