4ihi - Revision history

OCA at 11:57, 1 March 2024

OCA — Fri, 01 Mar 2024 11:57:09 GMT

←Older revision		Revision as of 11:57, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ihi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IHI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ihi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IHI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ihi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihi OCA], [https://pdbe.org/4ihi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ihi RCSB], [https://www.ebi.ac.uk/pdbsum/4ihi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ihi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihi OCA], [https://pdbe.org/4ihi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ihi RCSB], [https://www.ebi.ac.uk/pdbsum/4ihi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/O50443_MYCTU O50443_MYCTU]		[https://www.uniprot.org/uniprot/O50443_MYCTU O50443_MYCTU]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The proline-utilization pathway in Mycobacterium tuberculosis (Mtb) has recently been identified as an important factor in Mtb persistence in vivo, suggesting that this pathway could be a valuable therapeutic target against tuberculosis (TB). In Mtb, two distinct enzymes perform the conversion of proline into glutamate: the first step is the oxidation of proline into Delta(1)-pyrroline-5-carboxylic acid (P5C) by the flavoenzyme proline dehydrogenase (PruB), and the second reaction involves converting the tautomeric form of P5C (glutamate-gamma-semialdehyde) into glutamate using the NAD(+)-dependent Delta(1)-pyrroline-5-carboxylic dehydrogenase (PruA). Here, the three-dimensional structures of Mtb-PruA, determined by X-ray crystallography, in the apo state and in complex with NAD(+) are described at 2.5 and 2.1 A resolution, respectively. The structure reveals a conserved NAD(+)-binding mode, common to other related enzymes. Species-specific conformational differences in the active site, however, linked to changes in the dimer interface, suggest possibilities for selective inhibition of Mtb-PruA despite its reasonably high sequence identity to other PruA enzymes. Using recombinant PruA and PruB, the proline-utilization pathway in Mtb has also been reconstituted in vitro. Functional validation using a novel NMR approach has demonstrated that the PruA and PruB enzymes are together sufficient to convert proline to glutamate, the first such demonstration for monofunctional proline-utilization enzymes.
-
-	Characterization of the proline-utilization pathway in Mycobacterium tuberculosis through structural and functional studies.,Lagautriere T, Bashiri G, Paterson NG, Berney M, Cook GM, Baker EN Acta Crystallogr D Biol Crystallogr. 2014 Apr 1;70(Pt 4):968-80. doi:, 10.1107/S1399004713034391. Epub 2014 Mar 19. PMID:24699642<ref>PMID:24699642</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4ihi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Pyrroline-5-carboxylate dehydrogenase\|Pyrroline-5-carboxylate dehydrogenase]]		*[[Pyrroline-5-carboxylate dehydrogenase\|Pyrroline-5-carboxylate dehydrogenase]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 20:45, 16 November 2022

OCA — Wed, 16 Nov 2022 20:45:41 GMT

←Older revision		Revision as of 20:45, 16 November 2022
Line 1:		Line 1:

	==Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD==		==Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD==
-	<StructureSection load='4ihi' size='340' side='right' caption='[[4ihi]], [[Resolution\|resolution]] 2.25Å' scene=''>	+	<StructureSection load='4ihi' size='340' side='right'caption='[[4ihi]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ihi]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4IHI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ihi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IHI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4idm\|4idm]], [[4ids\|4ids]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ihi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihi OCA], [https://pdbe.org/4ihi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ihi RCSB], [https://www.ebi.ac.uk/pdbsum/4ihi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihi ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT1224, pruA, rocA, Rv1187 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-pyrroline-5-carboxylate_dehydrogenase 1-pyrroline-5-carboxylate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.12 1.5.1.12] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ihi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihi OCA], [~~http~~://pdbe.org/4ihi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ihi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ihi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihi ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O50443_MYCTU O50443_MYCTU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~1-pyrroline-5-carboxylate dehydrogenase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Baker~~, E N~~]]	+	[[Category: Baker EN]]
-	[[Category: Bashiri, G]]	+	[[Category: Bashiri G]]
-	[[Category: Lagautriere, T]]	+	[[Category: Lagautriere T]]
-	~~[[Category: Dehydrogenation]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Pyrroline-5-carboxylate dehtdrogenase]]~~	+
-	~~[[Category: Pyrroline-5-carboxylic acid]]~~	+
-	~~[[Category: Rossmann fold~~]]	+

OCA at 11:03, 11 August 2016

OCA — Thu, 11 Aug 2016 11:03:10 GMT

←Older revision		Revision as of 11:03, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD==		==Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD==
	<StructureSection load='4ihi' size='340' side='right' caption='[[4ihi]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='4ihi' size='340' side='right' caption='[[4ihi]], [[Resolution\|resolution]] 2.25Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT1224, pruA, rocA, Rv1187 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT1224, pruA, rocA, Rv1187 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-pyrroline-5-carboxylate_dehydrogenase 1-pyrroline-5-carboxylate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.12 1.5.1.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-pyrroline-5-carboxylate_dehydrogenase 1-pyrroline-5-carboxylate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.12 1.5.1.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ihi RCSB], [http://www.ebi.ac.uk/pdbsum/4ihi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihi OCA], [http://pdbe.org/4ihi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ihi RCSB], [http://www.ebi.ac.uk/pdbsum/4ihi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ihi ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ihi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 09:10, 25 January 2015

OCA — Sun, 25 Jan 2015 09:10:11 GMT

←Older revision		Revision as of 09:10, 25 January 2015
Line 2:		Line 2:
	<StructureSection load='4ihi' size='340' side='right' caption='[[4ihi]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='4ihi' size='340' side='right' caption='[[4ihi]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ihi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IHI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ihi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IHI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4idm\|4idm]], [[4ids\|4ids]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4idm\|4idm]], [[4ids\|4ids]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT1224, pruA, rocA, Rv1187 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-pyrroline-5-carboxylate_dehydrogenase 1-pyrroline-5-carboxylate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.12 1.5.1.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-pyrroline-5-carboxylate_dehydrogenase 1-pyrroline-5-carboxylate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.12 1.5.1.12] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ihi RCSB], [http://www.ebi.ac.uk/pdbsum/4ihi PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ihi RCSB], [http://www.ebi.ac.uk/pdbsum/4ihi PDBsum]</span></td></tr>
Line 24:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]		[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]
		+	[[Category: Myctu]]
	[[Category: Baker, E N]]		[[Category: Baker, E N]]
	[[Category: Bashiri, G]]		[[Category: Bashiri, G]]

OCA at 07:35, 12 November 2014

OCA — Wed, 12 Nov 2014 07:35:44 GMT

←Older revision		Revision as of 07:35, 12 November 2014
Line 2:		Line 2:
	<StructureSection load='4ihi' size='340' side='right' caption='[[4ihi]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='4ihi' size='340' side='right' caption='[[4ihi]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ihi]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IHI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ihi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IHI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4idm\|4idm]], [[4ids\|4ids]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4idm\|4idm]], [[4ids\|4ids]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT1224, pruA, rocA, Rv1187 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-pyrroline-5-carboxylate_dehydrogenase 1-pyrroline-5-carboxylate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.12 1.5.1.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-pyrroline-5-carboxylate_dehydrogenase 1-pyrroline-5-carboxylate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.12 1.5.1.12] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ihi RCSB], [http://www.ebi.ac.uk/pdbsum/4ihi PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ihi RCSB], [http://www.ebi.ac.uk/pdbsum/4ihi PDBsum]</span></td></tr>
Line 25:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]		[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]
-	~~[[Category: Myctu]]~~	+	[[Category: Baker, E N]]
-	[[Category: Baker, E N.]]	+	[[Category: Bashiri, G]]
-	[[Category: Bashiri, G.]]	+	[[Category: Lagautriere, T]]
-	[[Category: Lagautriere, T.]]	+
	[[Category: Dehydrogenation]]		[[Category: Dehydrogenation]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 14:24, 3 November 2014

OCA — Mon, 03 Nov 2014 14:24:36 GMT

←Older revision		Revision as of 14:24, 3 November 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4ihi\| PDB=4ihi \| SCENE= }}~~	+	==Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD==
-	===Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD===	+	<StructureSection load='4ihi' size='340' side='right' caption='[[4ihi]], [[Resolution\|resolution]] 2.25Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24699642}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ihi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IHI FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4idm\|4idm]], [[4ids\|4ids]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT1224, pruA, rocA, Rv1187 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-pyrroline-5-carboxylate_dehydrogenase 1-pyrroline-5-carboxylate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.12 1.5.1.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ihi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ihi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ihi RCSB], [http://www.ebi.ac.uk/pdbsum/4ihi PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The proline-utilization pathway in Mycobacterium tuberculosis (Mtb) has recently been identified as an important factor in Mtb persistence in vivo, suggesting that this pathway could be a valuable therapeutic target against tuberculosis (TB). In Mtb, two distinct enzymes perform the conversion of proline into glutamate: the first step is the oxidation of proline into Delta(1)-pyrroline-5-carboxylic acid (P5C) by the flavoenzyme proline dehydrogenase (PruB), and the second reaction involves converting the tautomeric form of P5C (glutamate-gamma-semialdehyde) into glutamate using the NAD(+)-dependent Delta(1)-pyrroline-5-carboxylic dehydrogenase (PruA). Here, the three-dimensional structures of Mtb-PruA, determined by X-ray crystallography, in the apo state and in complex with NAD(+) are described at 2.5 and 2.1 A resolution, respectively. The structure reveals a conserved NAD(+)-binding mode, common to other related enzymes. Species-specific conformational differences in the active site, however, linked to changes in the dimer interface, suggest possibilities for selective inhibition of Mtb-PruA despite its reasonably high sequence identity to other PruA enzymes. Using recombinant PruA and PruB, the proline-utilization pathway in Mtb has also been reconstituted in vitro. Functional validation using a novel NMR approach has demonstrated that the PruA and PruB enzymes are together sufficient to convert proline to glutamate, the first such demonstration for monofunctional proline-utilization enzymes.

-	~~==About this Structure==~~	+	Characterization of the proline-utilization pathway in Mycobacterium tuberculosis through structural and functional studies.,Lagautriere T, Bashiri G, Paterson NG, Berney M, Cook GM, Baker EN Acta Crystallogr D Biol Crystallogr. 2014 Apr 1;70(Pt 4):968-80. doi:, 10.1107/S1399004713034391. Epub 2014 Mar 19. PMID:24699642<ref>PMID:24699642</ref>
-	~~[[4ihi]] is a~~ 1 ~~chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Myctu Myctu]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:024699642</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+
		+	==See Also==
		+	*[[Pyrroline-5-carboxylate dehydrogenase\|Pyrroline-5-carboxylate dehydrogenase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]		[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]
	[[Category: Myctu]]		[[Category: Myctu]]
Line 17:		Line 33:
	[[Category: Pyrroline-5-carboxylate dehtdrogenase]]		[[Category: Pyrroline-5-carboxylate dehtdrogenase]]
	[[Category: Pyrroline-5-carboxylic acid]]		[[Category: Pyrroline-5-carboxylic acid]]
-	[[Category: ~~Rossman~~ fold]]	+	[[Category: Rossmann fold]]

OCA at 08:29, 16 April 2014

OCA — Wed, 16 Apr 2014 08:29:18 GMT

←Older revision		Revision as of 08:29, 16 April 2014
Line 1:		Line 1:
	{{STRUCTURE_4ihi\| PDB=4ihi \| SCENE= }}		{{STRUCTURE_4ihi\| PDB=4ihi \| SCENE= }}
	===Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD===		===Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD===
		+	{{ABSTRACT_PUBMED_24699642}}

	==About this Structure==		==About this Structure==
-	[[4ihi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA].	+	[[4ihi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024699642</ref><references group="xtra"/><references/>
	[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]		[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]
		+	[[Category: Myctu]]
	[[Category: Baker, E N.]]		[[Category: Baker, E N.]]
	[[Category: Bashiri, G.]]		[[Category: Bashiri, G.]]

OCA at 10:37, 18 December 2013

OCA — Wed, 18 Dec 2013 10:37:26 GMT

←Older revision		Revision as of 10:37, 18 December 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4ihi\| PDB=4ihi \| SCENE= }}
		+	===Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD===

-	~~The entry~~ 4ihi is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4ihi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IHI OCA].
-	~~Authors~~: ~~Lagautriere~~, T., Bashiri, G., ~~Baker, E.N~~.	+	[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]
-		+	[[Category: Baker, E N.]]
-	~~Description~~: ~~Crystal structure of the Delta-pyrroline~~-5-carboxylate ~~dehydrogenase from Mycobacterium tuberculosis bound with NAD~~	+	[[Category: Bashiri, G.]]
		+	[[Category: Lagautriere, T.]]
		+	[[Category: Dehydrogenation]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Pyrroline-5-carboxylate dehtdrogenase]]
		+	[[Category: Pyrroline-5-carboxylic acid]]
		+	[[Category: Rossman fold]]

OCA at 00:47, 4 April 2013

OCA — Thu, 04 Apr 2013 00:47:11 GMT

←Older revision		Revision as of 00:47, 4 April 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ihi is ON HOLD until ~~sometime in the future~~	+	The entry 4ihi is ON HOLD until Paper Publication

	Authors: Lagautriere, T., Bashiri, G., Baker, E.N.		Authors: Lagautriere, T., Bashiri, G., Baker, E.N.

	Description: Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD		Description: Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD

OCA at 07:50, 9 January 2013

OCA — Wed, 09 Jan 2013 07:50:03 GMT

←Older revision		Revision as of 07:50, 9 January 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ihi is ON HOLD	+	The entry 4ihi is ON HOLD until sometime in the future

	Authors: Lagautriere, T., Bashiri, G., Baker, E.N.		Authors: Lagautriere, T., Bashiri, G., Baker, E.N.

	Description: Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD		Description: Crystal structure of the Delta-pyrroline-5-carboxylate dehydrogenase from Mycobacterium tuberculosis bound with NAD