4ikz - Revision history

OCA at 19:23, 29 May 2024

OCA — Wed, 29 May 2024 19:23:29 GMT

←Older revision		Revision as of 19:23, 29 May 2024
Line 1:		Line 1:

	==Crystal structure of peptide transporter POT (E310Q mutant) in complex with alafosfalin==		==Crystal structure of peptide transporter POT (E310Q mutant) in complex with alafosfalin==
-	<StructureSection load='4ikz' size='340' side='right'caption='[[4ikz]]' scene=''>	+	<StructureSection load='4ikz' size='340' side='right'caption='[[4ikz]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IKZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ikz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IKZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ikz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ikz OCA], [https://pdbe.org/4ikz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ikz RCSB], [https://www.ebi.ac.uk/pdbsum/4ikz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ikz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AFS:N-[(1R)-1-PHOSPHONOETHYL]-L-ALANINAMIDE'>AFS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ikz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ikz OCA], [https://pdbe.org/4ikz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ikz RCSB], [https://www.ebi.ac.uk/pdbsum/4ikz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ikz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5KYD1_GEOKA Q5KYD1_GEOKA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proton-dependent oligopeptide transporters (POTs) are major facilitator superfamily (MFS) proteins that mediate the uptake of peptides and peptide-like molecules, using the inwardly directed H+ gradient across the membrane. The human POT family transporter peptide transporter 1 is present in the brush border membrane of the small intestine and is involved in the uptake of nutrient peptides and drug molecules such as beta-lactam antibiotics. Although previous studies have provided insight into the overall structure of the POT family transporters, the question of how transport is coupled to both peptide and H+ binding remains unanswered. Here we report the high-resolution crystal structures of a bacterial POT family transporter, including its complex with a dipeptide analog, alafosfalin. These structures revealed the key mechanistic and functional roles for a conserved glutamate residue (Glu310) in the peptide binding site. Integrated structural, biochemical, and computational analyses suggested a mechanism for H+-coupled peptide symport in which protonated Glu310 first binds the carboxyl group of the peptide substrate. The deprotonation of Glu310 in the inward open state triggers the release of the bound peptide toward the intracellular space and salt bridge formation between Glu310 and Arg43 to induce the state transition to the occluded conformation.
		+
		+	Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT.,Doki S, Kato HE, Solcan N, Iwaki M, Koyama M, Hattori M, Iwase N, Tsukazaki T, Sugita Y, Kandori H, Newstead S, Ishitani R, Nureki O Proc Natl Acad Sci U S A. 2013 Jun 24. PMID:23798427<ref>PMID:23798427</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4ikz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[ABC transporter 3D structures\|ABC transporter 3D structures]]		*[[ABC transporter 3D structures\|ABC transporter 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Geobacillus kaustophilus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Doki S]]		[[Category: Doki S]]

OCA at 10:44, 31 August 2022

OCA — Wed, 31 Aug 2022 10:44:02 GMT

←Older revision		Revision as of 10:44, 31 August 2022
Line 1:		Line 1:

	==Crystal structure of peptide transporter POT (E310Q mutant) in complex with alafosfalin==		==Crystal structure of peptide transporter POT (E310Q mutant) in complex with alafosfalin==
-	<StructureSection load='4ikz' size='340' side='right'caption='[[4ikz]]~~, [[Resolution\|resolution]] 2.40Å~~' scene=''>	+	<StructureSection load='4ikz' size='340' side='right'caption='[[4ikz]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[4ikz]] is a 1 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IKZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IKZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AFS:N-[(1R)-1-PHOSPHONOETHYL]-L-ALANINAMIDE'>AFS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ikz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ikz OCA], [https://pdbe.org/4ikz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ikz RCSB], [https://www.ebi.ac.uk/pdbsum/4ikz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ikz ProSAT]</span></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4ikv\|4ikv]], [[4ikw\|4ikw]], [[4ikx\|4ikx]], [[4iky\|4iky]]</div></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ikz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ikz OCA], [https://pdbe.org/4ikz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ikz RCSB], [https://www.ebi.ac.uk/pdbsum/4ikz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ikz ProSAT]</span></td></tr>	+
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Proton-dependent oligopeptide transporters (POTs) are major facilitator superfamily (MFS) proteins that mediate the uptake of peptides and peptide-like molecules, using the inwardly directed H+ gradient across the membrane. The human POT family transporter peptide transporter 1 is present in the brush border membrane of the small intestine and is involved in the uptake of nutrient peptides and drug molecules such as beta-lactam antibiotics. Although previous studies have provided insight into the overall structure of the POT family transporters, the question of how transport is coupled to both peptide and H+ binding remains unanswered. Here we report the high-resolution crystal structures of a bacterial POT family transporter, including its complex with a dipeptide analog, alafosfalin. These structures revealed the key mechanistic and functional roles for a conserved glutamate residue (Glu310) in the peptide binding site. Integrated structural, biochemical, and computational analyses suggested a mechanism for H+-coupled peptide symport in which protonated Glu310 first binds the carboxyl group of the peptide substrate. The deprotonation of Glu310 in the inward open state triggers the release of the bound peptide toward the intracellular space and salt bridge formation between Glu310 and Arg43 to induce the state transition to the occluded conformation.
-
-	Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT.,Doki S, Kato HE, Solcan N, Iwaki M, Koyama M, Hattori M, Iwase N, Tsukazaki T, Sugita Y, Kandori H, Newstead S, Ishitani R, Nureki O Proc Natl Acad Sci U S A. 2013 Jun 24. PMID:23798427<ref>PMID:23798427</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4ikz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[ABC transporter 3D structures\|ABC transporter 3D structures]]		*[[ABC transporter 3D structures\|ABC transporter 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Doki, S]]	+	[[Category: Doki S]]
-	[[Category: Ishitani, R]]	+	[[Category: Ishitani R]]
-	[[Category: Kato~~, H E~~]]	+	[[Category: Kato HE]]
-	[[Category: Nureki, O]]	+	[[Category: Nureki O]]
-	~~[[Category: Major facilitator superfamily]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 05:02, 25 August 2022

OCA — Thu, 25 Aug 2022 05:02:25 GMT

←Older revision		Revision as of 05:02, 25 August 2022
Line 1:		Line 1:

	==Crystal structure of peptide transporter POT (E310Q mutant) in complex with alafosfalin==		==Crystal structure of peptide transporter POT (E310Q mutant) in complex with alafosfalin==
-	<StructureSection load='4ikz' size='340' side='right' caption='[[4ikz]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='4ikz' size='340' side='right'caption='[[4ikz]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ikz]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"bacillus_kaustophilus"_prickett_1928 "bacillus kaustophilus" prickett 1928]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IKZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4IKZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ikz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IKZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AFS:N-[(1R)-1-PHOSPHONOETHYL]-L-ALANINAMIDE'>AFS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AFS:N-[(1R)-1-PHOSPHONOETHYL]-L-ALANINAMIDE'>AFS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ikv\|4ikv]], [[4ikw\|4ikw]], [[4ikx\|4ikx]], [[4iky\|4iky]]</td>~~</tr>~~	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4ikv\|4ikv]], [[4ikw\|4ikw]], [[4ikx\|4ikx]], [[4iky\|4iky]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GK2020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 "Bacillus kaustophilus" Prickett 1928])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ikz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ikz OCA], [https://pdbe.org/4ikz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ikz RCSB], [https://www.ebi.ac.uk/pdbsum/4ikz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ikz ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ikz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ikz OCA], [~~http~~://pdbe.org/4ikz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ikz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ikz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ikz ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 19:

	==See Also==		==See Also==
-	*[[ABC transporter\|ABC transporter]]	+	*[[ABC transporter 3D structures\|ABC transporter 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus kaustophilus prickett 1928~~]]	+	[[Category: Large Structures]]
	[[Category: Doki, S]]		[[Category: Doki, S]]
	[[Category: Ishitani, R]]		[[Category: Ishitani, R]]

OCA at 18:25, 11 August 2016

OCA — Thu, 11 Aug 2016 18:25:45 GMT

←Older revision		Revision as of 18:25, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of peptide transporter POT (E310Q mutant) in complex with alafosfalin==		==Crystal structure of peptide transporter POT (E310Q mutant) in complex with alafosfalin==
	<StructureSection load='4ikz' size='340' side='right' caption='[[4ikz]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4ikz' size='340' side='right' caption='[[4ikz]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ikz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Geobacillus_kaustophilus Geobacillus~~ kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IKZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IKZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ikz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_kaustophilus"_prickett_1928 "bacillus kaustophilus" prickett 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IKZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IKZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AFS:N-[(1R)-1-PHOSPHONOETHYL]-L-ALANINAMIDE'>AFS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AFS:N-[(1R)-1-PHOSPHONOETHYL]-L-ALANINAMIDE'>AFS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ikv\|4ikv]], [[4ikw\|4ikw]], [[4ikx\|4ikx]], [[4iky\|4iky]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ikv\|4ikv]], [[4ikw\|4ikw]], [[4ikx\|4ikx]], [[4iky\|4iky]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GK2020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 ~~Geobacillus~~ kaustophilus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GK2020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 "Bacillus kaustophilus" Prickett 1928])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ikz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ikz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ikz RCSB], [http://www.ebi.ac.uk/pdbsum/4ikz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ikz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ikz OCA], [http://pdbe.org/4ikz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ikz RCSB], [http://www.ebi.ac.uk/pdbsum/4ikz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ikz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ikz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Geobacillus~~ kaustophilus]]	+	[[Category: Bacillus kaustophilus prickett 1928]]
	[[Category: Doki, S]]		[[Category: Doki, S]]
	[[Category: Ishitani, R]]		[[Category: Ishitani, R]]

OCA at 13:17, 4 January 2015

OCA — Sun, 04 Jan 2015 13:17:24 GMT

←Older revision		Revision as of 13:17, 4 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4ikz\| PDB=4ikz \| SCENE= }}~~	+	==Crystal structure of peptide transporter POT (E310Q mutant) in complex with alafosfalin==
-	===Crystal structure of peptide transporter POT (E310Q mutant) in complex with alafosfalin===	+	<StructureSection load='4ikz' size='340' side='right' caption='[[4ikz]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23798427}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ikz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IKZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IKZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AFS:N-[(1R)-1-PHOSPHONOETHYL]-L-ALANINAMIDE'>AFS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ikv\|4ikv]], [[4ikw\|4ikw]], [[4ikx\|4ikx]], [[4iky\|4iky]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GK2020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 Geobacillus kaustophilus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ikz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ikz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ikz RCSB], [http://www.ebi.ac.uk/pdbsum/4ikz PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proton-dependent oligopeptide transporters (POTs) are major facilitator superfamily (MFS) proteins that mediate the uptake of peptides and peptide-like molecules, using the inwardly directed H+ gradient across the membrane. The human POT family transporter peptide transporter 1 is present in the brush border membrane of the small intestine and is involved in the uptake of nutrient peptides and drug molecules such as beta-lactam antibiotics. Although previous studies have provided insight into the overall structure of the POT family transporters, the question of how transport is coupled to both peptide and H+ binding remains unanswered. Here we report the high-resolution crystal structures of a bacterial POT family transporter, including its complex with a dipeptide analog, alafosfalin. These structures revealed the key mechanistic and functional roles for a conserved glutamate residue (Glu310) in the peptide binding site. Integrated structural, biochemical, and computational analyses suggested a mechanism for H+-coupled peptide symport in which protonated Glu310 first binds the carboxyl group of the peptide substrate. The deprotonation of Glu310 in the inward open state triggers the release of the bound peptide toward the intracellular space and salt bridge formation between Glu310 and Arg43 to induce the state transition to the occluded conformation.

-	~~==About this Structure==~~	+	Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT.,Doki S, Kato HE, Solcan N, Iwaki M, Koyama M, Hattori M, Iwase N, Tsukazaki T, Sugita Y, Kandori H, Newstead S, Ishitani R, Nureki O Proc Natl Acad Sci U S A. 2013 Jun 24. PMID:23798427<ref>PMID:23798427</ref>
-	~~[[4ikz]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4IKZ OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:023798427</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+
		+	==See Also==
		+	*[[ABC transporter\|ABC transporter]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Geobacillus kaustophilus]]		[[Category: Geobacillus kaustophilus]]
-	[[Category: Doki, S.]]	+	[[Category: Doki, S]]
-	[[Category: Ishitani, R.]]	+	[[Category: Ishitani, R]]
-	[[Category: Kato, H E.]]	+	[[Category: Kato, H E]]
-	[[Category: Nureki, O.]]	+	[[Category: Nureki, O]]
	[[Category: Major facilitator superfamily]]		[[Category: Major facilitator superfamily]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 15:39, 10 July 2013

OCA — Wed, 10 Jul 2013 15:39:02 GMT

←Older revision		Revision as of 15:39, 10 July 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4ikz\| PDB=4ikz \| SCENE= }}
		+	===Crystal structure of peptide transporter POT (E310Q mutant) in complex with alafosfalin===
		+	{{ABSTRACT_PUBMED_23798427}}

-	~~The entry~~ 4ikz is ~~ON HOLD~~	+	==About this Structure==
		+	[[4ikz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IKZ OCA].

-	~~Authors~~: Doki, S., Kato, H.E.~~, Ishitani, R.,~~ Nureki, O.	+	==Reference==
-		+	<ref group="xtra">PMID:023798427</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal structure of~~ protein	+	[[Category: Geobacillus kaustophilus]]
		+	[[Category: Doki, S.]]
		+	[[Category: Ishitani, R.]]
		+	[[Category: Kato, H E.]]
		+	[[Category: Nureki, O.]]
		+	[[Category: Major facilitator superfamily]]
		+	[[Category: Transport protein]]

OCA: Protected "4ikz" [edit=sysop:move=sysop]

OCA — Wed, 09 Jan 2013 07:51:08 GMT

Protected "4ikz" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:51, 9 January 2013

OCA: New page: '''Unreleased structure''' The entry 4ikz is ON HOLD Authors: Doki, S., Kato, H.E., Ishitani, R., Nureki, O. Description: Crystal structure of protein

OCA — Wed, 09 Jan 2013 07:51:07 GMT

New page: '''Unreleased structure''' The entry 4ikz is ON HOLD Authors: Doki, S., Kato, H.E., Ishitani, R., Nureki, O. Description: Crystal structure of protein

New page

'''Unreleased structure'''

The entry 4ikz is ON HOLD

Authors: Doki, S., Kato, H.E., Ishitani, R., Nureki, O.

Description: Crystal structure of protein