4ipm - Revision history

OCA at 14:17, 8 November 2023

OCA — Wed, 08 Nov 2023 14:17:30 GMT

←Older revision		Revision as of 14:17, 8 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ipm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Limnoria_quadripunctata Limnoria quadripunctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IPM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ipm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Limnoria_quadripunctata Limnoria quadripunctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IPM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=PRD_900034:thio-beta-cellobiose'>PRD_900034</scene>, <scene name='pdbligand=SGC:4-DEOXY-4-THIO-BETA-D-GLUCOPYRANOSE'>SGC</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.14Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=PRD_900034:thio-beta-cellobiose'>PRD_900034</scene>, <scene name='pdbligand=SGC:4-DEOXY-4-THIO-BETA-D-GLUCOPYRANOSE'>SGC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ipm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ipm OCA], [https://pdbe.org/4ipm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ipm RCSB], [https://www.ebi.ac.uk/pdbsum/4ipm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ipm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ipm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ipm OCA], [https://pdbe.org/4ipm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ipm RCSB], [https://www.ebi.ac.uk/pdbsum/4ipm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ipm ProSAT]</span></td></tr>
	</table>		</table>

OCA at 21:02, 16 November 2022

OCA — Wed, 16 Nov 2022 21:02:25 GMT

←Older revision		Revision as of 21:02, 16 November 2022
Line 3:		Line 3:
	<StructureSection load='4ipm' size='340' side='right'caption='[[4ipm]], [[Resolution\|resolution]] 1.14Å' scene=''>		<StructureSection load='4ipm' size='340' side='right'caption='[[4ipm]], [[Resolution\|resolution]] 1.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ipm]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Limnoria_quadripunctata Limnoria quadripunctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4IPM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ipm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Limnoria_quadripunctata Limnoria quadripunctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IPM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:~~CALCIUM+ION~~'>CA</scene>, <scene name='pdbligand=~~TCB~~:~~THIOCELLOBIOSE~~'>~~TCB~~</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=PRD_900034:thio-beta-cellobiose'>PRD_900034</scene>, <scene name='pdbligand=SGC:4-DEOXY-4-THIO-BETA-D-GLUCOPYRANOSE'>SGC</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene><~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ipm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ipm OCA], [https://pdbe.org/4ipm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ipm RCSB], [https://www.ebi.ac.uk/pdbsum/4ipm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ipm ProSAT]</span></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat">[[4gwa\|4gwa]]~~, ~~[[4hap\|4hap]], [[4haq\|4haq]]~~<~~/td></tr>~~	+
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">GH7B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=161573 Limnoria quadripunctata])</td></tr>~~	+
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,~~4-~~beta~~-~~cellobiosidase_(non-reducing_end) Cellulose 1,~~4-~~beta~~-~~cellobiosidase (non~~-~~reducing end)], with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ipm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ipm OCA], [~~http~~://pdbe.org/4ipm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ipm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ipm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ipm ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GH7B_LIMQU GH7B_LIMQU] Exocellobiohydrolase (CBH) that catalyzes the hydrolysis of 1,4-beta-D-glucosidic bonds in cellulose to release the disaccharide cellobiose (PubMed:23733951). The degradation of cellulose involves an interplay between different cellulolytic enzymes. Hydrolysis starts with endoglucanases (EGs), which cut internal beta-1,4-glucosidic bonds in cellulose to reduce the polymerization degree of the substrate and create new chain ends for exocellobiohydrolases (CBHs). The CBHs release the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain. Finally, beta-1,4-glucosidases hydrolyze the cellobiose and other short cello-oligosaccharides into glucose units (Probable).<ref>PMID:23733951</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 24:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Limnoria quadripunctata]]		[[Category: Limnoria quadripunctata]]
-	[[Category: Bruce~~, N C~~]]	+	[[Category: Bruce NC]]
-	[[Category: Cragg~~, S M~~]]	+	[[Category: Cragg SM]]
-	[[Category: Guille~~, M J~~]]	+	[[Category: Guille MJ]]
-	[[Category: Kern, M]]	+	[[Category: Kern M]]
-	[[Category: Martin~~, R N.A~~]]	+	[[Category: Martin RNA]]
-	[[Category: McGeehan~~, J E~~]]	+	[[Category: McGeehan JE]]
-	[[Category: McQueen-Mason~~, S J~~]]	+	[[Category: McQueen-Mason SJ]]
-	[[Category: Schnorr~~, K M~~]]	+	[[Category: Schnorr KM]]
-	[[Category: Streeter~~, S D]]~~	+	[[Category: Streeter SD]]
-	~~[[Category: Cellobiohydrolase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Thiocellobiose~~]]	+

OCA at 10:02, 25 December 2019

OCA — Wed, 25 Dec 2019 10:02:24 GMT

←Older revision		Revision as of 10:02, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with thiocellobiose==		==Crystal structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with thiocellobiose==
-	<StructureSection load='4ipm' size='340' side='right' caption='[[4ipm]], [[Resolution\|resolution]] 1.14Å' scene=''>	+	<StructureSection load='4ipm' size='340' side='right'caption='[[4ipm]], [[Resolution\|resolution]] 1.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ipm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Limnoria_quadripunctata Limnoria quadripunctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IPM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ipm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Limnoria_quadripunctata Limnoria quadripunctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IPM FirstGlance]. <br>
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Limnoria quadripunctata]]		[[Category: Limnoria quadripunctata]]
	[[Category: Bruce, N C]]		[[Category: Bruce, N C]]

OCA at 16:33, 11 August 2016

OCA — Thu, 11 Aug 2016 16:33:56 GMT

←Older revision		Revision as of 16:33, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with thiocellobiose==		==Crystal structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with thiocellobiose==
	<StructureSection load='4ipm' size='340' side='right' caption='[[4ipm]], [[Resolution\|resolution]] 1.14Å' scene=''>		<StructureSection load='4ipm' size='340' side='right' caption='[[4ipm]], [[Resolution\|resolution]] 1.14Å' scene=''>
Line 8:		Line 9:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GH7B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=161573 Limnoria quadripunctata])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GH7B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=161573 Limnoria quadripunctata])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ipm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ipm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ipm RCSB], [http://www.ebi.ac.uk/pdbsum/4ipm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ipm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ipm OCA], [http://pdbe.org/4ipm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ipm RCSB], [http://www.ebi.ac.uk/pdbsum/4ipm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ipm ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ipm" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:52, 5 January 2015

OCA — Mon, 05 Jan 2015 14:52:16 GMT

←Older revision		Revision as of 14:52, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ipm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Limnoria_quadripunctata Limnoria quadripunctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IPM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ipm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Limnoria_quadripunctata Limnoria quadripunctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IPM FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=TCB:THIOCELLOBIOSE'>TCB</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=TCB:THIOCELLOBIOSE'>TCB</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gwa\|4gwa]], [[4hap\|4hap]], [[4haq\|4haq]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gwa\|4gwa]], [[4hap\|4hap]], [[4haq\|4haq]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GH7B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=161573 Limnoria quadripunctata])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GH7B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=161573 Limnoria quadripunctata])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ipm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ipm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ipm RCSB], [http://www.ebi.ac.uk/pdbsum/4ipm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ipm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ipm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ipm RCSB], [http://www.ebi.ac.uk/pdbsum/4ipm PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Limnoria quadripunctata]]		[[Category: Limnoria quadripunctata]]
-	[[Category: Bruce, N C.]]	+	[[Category: Bruce, N C]]
-	[[Category: Cragg, S M.]]	+	[[Category: Cragg, S M]]
-	[[Category: Guille, M J.]]	+	[[Category: Guille, M J]]
-	[[Category: Kern, M.]]	+	[[Category: Kern, M]]
-	[[Category: Martin, R N.A.]]	+	[[Category: Martin, R N.A]]
-	[[Category: McGeehan, J E.]]	+	[[Category: McGeehan, J E]]
-	[[Category: McQueen-Mason, S J.]]	+	[[Category: McQueen-Mason, S J]]
-	[[Category: Schnorr, K M.]]	+	[[Category: Schnorr, K M]]
-	[[Category: Streeter, S D.]]	+	[[Category: Streeter, S D]]
	[[Category: Cellobiohydrolase]]		[[Category: Cellobiohydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Thiocellobiose]]		[[Category: Thiocellobiose]]

OCA at 02:59, 4 September 2014

OCA — Thu, 04 Sep 2014 02:59:03 GMT

←Older revision		Revision as of 02:59, 4 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4ipm\| PDB=4ipm \| SCENE= }}~~	+	==Crystal structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with thiocellobiose==
-	===Crystal structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with thiocellobiose===	+	<StructureSection load='4ipm' size='340' side='right' caption='[[4ipm]], [[Resolution\|resolution]] 1.14Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20212162}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ipm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Limnoria_quadripunctata Limnoria quadripunctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IPM FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=TCB:THIOCELLOBIOSE'>TCB</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gwa\|4gwa]], [[4hap\|4hap]], [[4haq\|4haq]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GH7B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=161573 Limnoria quadripunctata])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ipm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ipm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ipm RCSB], [http://www.ebi.ac.uk/pdbsum/4ipm PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nature uses a diversity of glycoside hydrolase (GH) enzymes to convert polysaccharides to sugars. As lignocellulosic biomass deconstruction for biofuel production remains costly, natural GH diversity offers a starting point for developing industrial enzymes, and fungal GH family 7 (GH7) cellobiohydrolases, in particular, provide significant hydrolytic potential in industrial mixtures. Recently, GH7 enzymes have been found in other kingdoms of life besides fungi, including in animals and protists. Here, we describe the in vivo spatial expression distribution, properties, and structure of a unique endogenous GH7 cellulase from an animal, the marine wood borer Limnoria quadripunctata (LqCel7B). RT-quantitative PCR and Western blot studies show that LqCel7B is expressed in the hepatopancreas and secreted into the gut for wood degradation. We produced recombinant LqCel7B, with which we demonstrate that LqCel7B is a cellobiohydrolase and obtained four high-resolution crystal structures. Based on a crystallographic and computational comparison of LqCel7B to the well-characterized Hypocrea jecorina GH7 cellobiohydrolase, LqCel7B exhibits an extended substrate-binding motif at the tunnel entrance, which may aid in substrate acquisition and processivity. Interestingly, LqCel7B exhibits striking surface charges relative to fungal GH7 enzymes, which likely results from evolution in marine environments. We demonstrate that LqCel7B stability and activity remain unchanged, or increase at high salt concentration, and that the L. quadripunctata GH mixture generally contains cellulolytic enzymes with highly acidic surface charge compared with enzymes derived from terrestrial microbes. Overall, this study suggests that marine cellulases offer significant potential for utilization in high-solids industrial biomass conversion processes.

-	~~==About this Structure==~~	+	Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance.,Kern M, McGeehan JE, Streeter SD, Martin RN, Besser K, Elias L, Eborall W, Malyon GP, Payne CM, Himmel ME, Schnorr K, Beckham GT, Cragg SM, Bruce NC, McQueen-Mason SJ Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10189-94. doi:, 10.1073/pnas.1301502110. Epub 2013 Jun 3. PMID:23733951<ref>PMID:23733951</ref>
-	~~[[4ipm]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Limnoria_quadripunctata Limnoria quadripunctata]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4IPM OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:020212162</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Limnoria quadripunctata]]		[[Category: Limnoria quadripunctata]]
	[[Category: Bruce, N C.]]		[[Category: Bruce, N C.]]

OCA at 21:04, 19 June 2013

OCA — Wed, 19 Jun 2013 21:04:04 GMT

←Older revision		Revision as of 21:04, 19 June 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4ipm\| PDB=4ipm \| SCENE= }}
		+	===Crystal structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with thiocellobiose===
		+	{{ABSTRACT_PUBMED_20212162}}

-	~~The entry~~ 4ipm is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4ipm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Limnoria_quadripunctata Limnoria quadripunctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPM OCA].

-	~~Authors~~: ~~McGeehan~~, ~~J.E., Martin, R.~~N.~~A., Streeter, S.D.,~~ Cragg, S.M., Guille, M.J.~~, Schnorr, K.M.,~~ Kern, M., ~~Bruce,~~ N.C., McQueen-Mason, S.J.	+	==Reference==
-		+	<ref group="xtra">PMID:020212162</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with thiocellobiose~~	+	[[Category: Limnoria quadripunctata]]
		+	[[Category: Bruce, N C.]]
		+	[[Category: Cragg, S M.]]
		+	[[Category: Guille, M J.]]
		+	[[Category: Kern, M.]]
		+	[[Category: Martin, R N.A.]]
		+	[[Category: McGeehan, J E.]]
		+	[[Category: McQueen-Mason, S J.]]
		+	[[Category: Schnorr, K M.]]
		+	[[Category: Streeter, S D.]]
		+	[[Category: Cellobiohydrolase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Thiocellobiose]]

OCA at 10:05, 13 February 2013

OCA — Wed, 13 Feb 2013 10:05:43 GMT

←Older revision		Revision as of 10:05, 13 February 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ipm is ON HOLD	+	The entry 4ipm is ON HOLD until Paper Publication

	Authors: McGeehan, J.E., Martin, R.N.A., Streeter, S.D., Cragg, S.M., Guille, M.J., Schnorr, K.M., Kern, M., Bruce, N.C., McQueen-Mason, S.J.		Authors: McGeehan, J.E., Martin, R.N.A., Streeter, S.D., Cragg, S.M., Guille, M.J., Schnorr, K.M., Kern, M., Bruce, N.C., McQueen-Mason, S.J.

	Description: Crystal structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with thiocellobiose		Description: Crystal structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with thiocellobiose

OCA: Protected "4ipm" [edit=sysop:move=sysop]

OCA — Wed, 16 Jan 2013 08:29:07 GMT

Protected "4ipm" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:29, 16 January 2013

OCA: New page: '''Unreleased structure''' The entry 4ipm is ON HOLD Authors: McGeehan, J.E., Martin, R.N.A., Streeter, S.D., Cragg, S.M., Guille, M.J., Schnorr, K.M., Kern, M., Bruce, N.C., McQueen-Ma...

OCA — Wed, 16 Jan 2013 08:29:06 GMT

New page: '''Unreleased structure''' The entry 4ipm is ON HOLD Authors: McGeehan, J.E., Martin, R.N.A., Streeter, S.D., Cragg, S.M., Guille, M.J., Schnorr, K.M., Kern, M., Bruce, N.C., McQueen-Ma...

New page

'''Unreleased structure'''

The entry 4ipm is ON HOLD

Authors: McGeehan, J.E., Martin, R.N.A., Streeter, S.D., Cragg, S.M., Guille, M.J., Schnorr, K.M., Kern, M., Bruce, N.C., McQueen-Mason, S.J.

Description: Crystal structure of a GH7 family cellobiohydrolase from Limnoria quadripunctata in complex with thiocellobiose