4iss - Revision history

OCA at 21:08, 16 November 2022

OCA — Wed, 16 Nov 2022 21:08:31 GMT

←Older revision		Revision as of 21:08, 16 November 2022
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	==SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase==		==SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase==
-	<StructureSection load='4iss' size='340' side='right' caption='[[4iss]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4iss' size='340' side='right'caption='[[4iss]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4iss]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Candida_sphaerica Candida sphaerica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ISS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4iss]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ISS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=~~TAR~~:~~D(-)-TARTARIC+ACID~~'>~~TAR~~</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=TAR:D(-)-TARTARIC+ACID'>TAR</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE'>MSE</scene></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iss OCA], [https://pdbe.org/4iss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iss RCSB], [https://www.ebi.ac.uk/pdbsum/4iss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iss ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ist\|4ist]]</td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KLLA0E08119g, KLLA0_E08119g~~ (~~[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28985 Candida sphaerica]~~)~~</td></tr>~~	+
-	~~<tr id='activity~~'>~~<td class="sblockLbl"><b>Activity:~~</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Allophanate_hydrolase Allophanate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.54 3.5.1.54] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4iss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iss OCA], [~~http~~://pdbe.org/4iss PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4iss RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4iss PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4iss ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6CP22_KLULA Q6CP22_KLULA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Allophanate hydrolase~~]]	+	[[Category: Kluyveromyces lactis]]
-	[[Category: ~~Candida sphaerica~~]]	+	[[Category: Large Structures]]
-	[[Category: Fan, C]]	+	[[Category: Fan C]]
-	[[Category: Xiang, S]]	+	[[Category: Xiang S]]
-	~~[[Category: Allophanate binding]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mixed alpha and beta structure~~]]	+

OCA at 04:57, 5 August 2016

OCA — Fri, 05 Aug 2016 04:57:24 GMT

←Older revision		Revision as of 04:57, 5 August 2016
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		+
	==SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase==		==SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase==
	<StructureSection load='4iss' size='340' side='right' caption='[[4iss]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4iss' size='340' side='right' caption='[[4iss]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4iss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Kluyveromyces_lactis Kluyveromyces lactis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ISS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4iss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_sphaerica Candida sphaerica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ISS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TAR:D(-)-TARTARIC+ACID'>TAR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TAR:D(-)-TARTARIC+ACID'>TAR</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ist\|4ist]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ist\|4ist]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KLLA0E08119g, KLLA0_E08119g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28985 ~~Kluyveromyces lactis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KLLA0E08119g, KLLA0_E08119g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28985 Candida sphaerica])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Allophanate_hydrolase Allophanate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.54 3.5.1.54] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Allophanate_hydrolase Allophanate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.54 3.5.1.54] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iss OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iss RCSB], [http://www.ebi.ac.uk/pdbsum/4iss PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iss OCA], [http://pdbe.org/4iss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4iss RCSB], [http://www.ebi.ac.uk/pdbsum/4iss PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4iss ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4iss" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Allophanate hydrolase]]		[[Category: Allophanate hydrolase]]
-	[[Category: ~~Kluyveromyces lactis~~]]	+	[[Category: Candida sphaerica]]
	[[Category: Fan, C]]		[[Category: Fan, C]]
	[[Category: Xiang, S]]		[[Category: Xiang, S]]

OCA at 13:01, 21 December 2014

OCA — Sun, 21 Dec 2014 13:01:21 GMT

←Older revision		Revision as of 13:01, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4iss\| PDB~~=4iss \| ~~SCENE~~= }}	+	==SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase==
-	===~~SeMet~~-~~substituted~~ Kluyveromyces lactis Allophanate ~~Hydrolase~~===	+	<StructureSection load='4iss' size='340' side='right' caption='[[4iss]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23754281}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4iss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ISS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TAR:D(-)-TARTARIC+ACID'>TAR</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ist\|4ist]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KLLA0E08119g, KLLA0_E08119g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28985 Kluyveromyces lactis])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Allophanate_hydrolase Allophanate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.54 3.5.1.54] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iss OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iss RCSB], [http://www.ebi.ac.uk/pdbsum/4iss PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Allophanate hydrolase (AH) converts allophanate to ammonium and carbon dioxide. It is conserved in many organisms, and is essential for their utilization of urea as the nitrogen source. It also has important functions in a newly discovered eukaryotic pyrimidine nucleic acid precursor degradation pathway, the yeast-hyphal transition that several pathogens utilize to escape the host defense, and an s-triazine herbicides degradation pathway recently emerged in many soil bacteria. We have determined the crystal structure of the Kluyveromyces lactis AH. Together with structure directed functional studies, we demonstrate that its N and C domains catalyze a two-step reaction, and contribute to maintaining a dimeric form of the enzyme, required for their optimal activities. Our studies also brought molecular insights into their catalytic mechanism. Interestingly, we found that the C domain probably catalyzes a novel form of decarboxylation reaction, which might expand the knowledge of this common reaction in biological systems.

-	~~==About this~~ Structure==	+	Structure and Function of Allophanate Hydrolase.,Fan C, Li Z, Yin H, Xiang S J Biol Chem. 2013 Jun 10. PMID:23754281<ref>PMID:23754281</ref>
-	~~[[4iss]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4ISS OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023754281</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Allophanate hydrolase]]		[[Category: Allophanate hydrolase]]
	[[Category: Kluyveromyces lactis]]		[[Category: Kluyveromyces lactis]]
-	[[Category: Fan, C.]]	+	[[Category: Fan, C]]
-	[[Category: Xiang, S.]]	+	[[Category: Xiang, S]]
	[[Category: Allophanate binding]]		[[Category: Allophanate binding]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Mixed alpha and beta structure]]		[[Category: Mixed alpha and beta structure]]

OCA at 07:42, 30 June 2013

OCA — Sun, 30 Jun 2013 07:42:22 GMT

←Older revision		Revision as of 07:42, 30 June 2013
Line 1:		Line 1:
	{{STRUCTURE_4iss\| PDB=4iss \| SCENE= }}		{{STRUCTURE_4iss\| PDB=4iss \| SCENE= }}
	===SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase===		===SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase===
		+	{{ABSTRACT_PUBMED_23754281}}

	==About this Structure==		==About this Structure==
	[[4iss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISS OCA].		[[4iss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISS OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023754281</ref><references group="xtra"/><references/>
	[[Category: Allophanate hydrolase]]		[[Category: Allophanate hydrolase]]
	[[Category: Kluyveromyces lactis]]		[[Category: Kluyveromyces lactis]]

OCA at 07:24, 20 June 2013

OCA — Thu, 20 Jun 2013 07:24:50 GMT

←Older revision		Revision as of 07:24, 20 June 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4iss\| PDB=4iss \| SCENE= }}
		+	===SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase===

-	~~The entry~~ 4iss is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4iss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ISS OCA].
-	~~Authors~~: Fan, C., Xiang, S.	+	[[Category: Allophanate hydrolase]]
-		+	[[Category: Kluyveromyces lactis]]
-	~~Description~~: ~~SeMet-substituted Kluyveromyces lactis~~ Allophanate Hydrolase	+	[[Category: Fan, C.]]
		+	[[Category: Xiang, S.]]
		+	[[Category: Allophanate binding]]
		+	[[Category: Hydrolase]]
		+	[[Category: Mixed alpha and beta structure]]

OCA at 12:28, 6 February 2013

OCA — Wed, 06 Feb 2013 12:28:05 GMT

←Older revision		Revision as of 12:28, 6 February 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4iss is ON HOLD	+	The entry 4iss is ON HOLD until Paper Publication

-	Authors: ~~Chen~~, F., ~~Song~~, X.	+	Authors: Fan, C., Xiang, S.

	Description: SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase		Description: SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase

OCA at 12:03, 30 January 2013

OCA — Wed, 30 Jan 2013 12:03:50 GMT

←Older revision		Revision as of 12:03, 30 January 2013
Line 3:		Line 3:
	The entry 4iss is ON HOLD		The entry 4iss is ON HOLD

-	Authors: Chen ~~Fan~~, Song ~~Xiang~~	+	Authors: Chen, F., Song, X.

	Description: SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase		Description: SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase

OCA: Protected "4iss" [edit=sysop:move=sysop]

OCA — Wed, 23 Jan 2013 11:15:34 GMT

Protected "4iss" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:15, 23 January 2013

OCA: New page: '''Unreleased structure''' The entry 4iss is ON HOLD Authors: Chen Fan, Song Xiang Description: SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase

OCA — Wed, 23 Jan 2013 11:15:33 GMT

New page: '''Unreleased structure''' The entry 4iss is ON HOLD Authors: Chen Fan, Song Xiang Description: SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase

New page

'''Unreleased structure'''

The entry 4iss is ON HOLD

Authors: Chen Fan, Song Xiang

Description: SeMet-substituted Kluyveromyces lactis Allophanate Hydrolase