4iuc - Revision history

OCA at 15:31, 20 September 2023

OCA — Wed, 20 Sep 2023 15:31:09 GMT

←Older revision		Revision as of 15:31, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4iuc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_necator_H16 Cupriavidus necator H16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IUC FirstGlance]. <br>		<table><tr><td colspan='2'>[[4iuc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_necator_H16 Cupriavidus necator H16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IUC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NFV:NI-FE+OXIDIZED+ACTIVE+CENTER'>NFV</scene>, <scene name='pdbligand=S3F:OXIDIZED+[FE4-S3]+CLUSTER'>S3F</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NFV:NI-FE+OXIDIZED+ACTIVE+CENTER'>NFV</scene>, <scene name='pdbligand=S3F:OXIDIZED+[FE4-S3]+CLUSTER'>S3F</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iuc OCA], [https://pdbe.org/4iuc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iuc RCSB], [https://www.ebi.ac.uk/pdbsum/4iuc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iuc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iuc OCA], [https://pdbe.org/4iuc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iuc RCSB], [https://www.ebi.ac.uk/pdbsum/4iuc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iuc ProSAT]</span></td></tr>
	</table>		</table>

OCA at 21:11, 16 November 2022

OCA — Wed, 16 Nov 2022 21:11:26 GMT

←Older revision		Revision as of 21:11, 16 November 2022
Line 1:		Line 1:

	==Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 2==		==Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 2==
-	<StructureSection load='4iuc' size='340' side='right' caption='[[4iuc]], [[Resolution\|resolution]] 1.45Å' scene=''>	+	<StructureSection load='4iuc' size='340' side='right'caption='[[4iuc]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4iuc]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Alcaligenes_eutropha_h16 Alcaligenes eutropha h16~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4IUC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4iuc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_necator_H16 Cupriavidus necator H16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IUC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NFV:NI-FE+OXIDIZED+ACTIVE+CENTER'>NFV</scene>, <scene name='pdbligand=S3F:OXIDIZED+[FE4-S3]+CLUSTER'>S3F</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NFV:NI-FE+OXIDIZED+ACTIVE+CENTER'>NFV</scene>, <scene name='pdbligand=S3F:OXIDIZED+[FE4-S3]+CLUSTER'>S3F</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4iub\|4iub]], [[4iud\|4iud]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iuc OCA], [https://pdbe.org/4iuc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iuc RCSB], [https://www.ebi.ac.uk/pdbsum/4iuc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iuc ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hoxG, PHG002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16]), hoxK, PHG001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen:quinone_oxidoreductase Hydrogen:quinone oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.5.1 1.12.5.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4iuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iuc OCA], [~~http~~://pdbe.org/4iuc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4iuc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4iuc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4iuc ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MBHL_CUPNH MBHL_CUPNH]] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions. [[http://www.uniprot.org/uniprot/MBHS_CUPNH MBHS_CUPNH]] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.	+	[https://www.uniprot.org/uniprot/MBHL_CUPNH MBHL_CUPNH] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Alcaligenes eutropha h16~~]]	+	[[Category: Cupriavidus necator H16]]
-	[[Category: ~~Hydrogen:quinone oxidoreductase~~]]	+	[[Category: Large Structures]]
-	[[Category: Frielingsdorf, S]]	+	[[Category: Frielingsdorf S]]
-	[[Category: Fritsch, J]]	+	[[Category: Fritsch J]]
-	[[Category: Lenz, O]]	+	[[Category: Lenz O]]
-	[[Category: Scheerer, P]]	+	[[Category: Scheerer P]]
-	[[Category: Schmidt, A]]	+	[[Category: Schmidt A]]
-	~~[[Category: Aerobic hydrogen bacteria]]~~	+
-	~~[[Category: Bimetallic]]~~	+
-	~~[[Category: Hydrogen catalysis]]~~	+
-	~~[[Category: Iron-sulfur cluster]]~~	+
-	~~[[Category: Knallgas bacteria]]~~	+
-	~~[[Category: Membrane-bound]]~~	+
-	~~[[Category: Metalloenzyme]]~~	+
-	~~[[Category: Metalloprotein catalytic center]]~~	+
-	~~[[Category: Ni-fe active site]]~~	+
-	~~[[Category: Nickel-iron cofactor]]~~	+
-	~~[[Category: Oxidized state]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Oxygen-tolerant hydrogenase]]~~	+
-	~~[[Category: Proteobacteria]]~~	+
-	~~[[Category: Reduced state~~]]	+

OCA at 19:07, 5 August 2016

OCA — Fri, 05 Aug 2016 19:07:25 GMT

←Older revision		Revision as of 19:07, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 2==		==Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 2==
	<StructureSection load='4iuc' size='340' side='right' caption='[[4iuc]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='4iuc' size='340' side='right' caption='[[4iuc]], [[Resolution\|resolution]] 1.45Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hoxG, PHG002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16]), hoxK, PHG001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hoxG, PHG002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16]), hoxK, PHG001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen:quinone_oxidoreductase Hydrogen:quinone oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.5.1 1.12.5.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen:quinone_oxidoreductase Hydrogen:quinone oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.5.1 1.12.5.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iuc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iuc RCSB], [http://www.ebi.ac.uk/pdbsum/4iuc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iuc OCA], [http://pdbe.org/4iuc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4iuc RCSB], [http://www.ebi.ac.uk/pdbsum/4iuc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4iuc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4iuc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:12, 25 December 2014

OCA — Thu, 25 Dec 2014 21:12:04 GMT

←Older revision		Revision as of 21:12, 25 December 2014
Line 2:		Line 2:
	<StructureSection load='4iuc' size='340' side='right' caption='[[4iuc]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='4iuc' size='340' side='right' caption='[[4iuc]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4iuc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_eutropha_h16 Alcaligenes eutropha h16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUC OCA]. <br>	+	<table><tr><td colspan='2'>[[4iuc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_eutropha_h16 Alcaligenes eutropha h16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IUC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NFV:NI-FE+OXIDIZED+ACTIVE+CENTER'>NFV</scene>, <scene name='pdbligand=S3F:OXIDIZED+[FE4-S3]+CLUSTER'>S3F</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NFV:NI-FE+OXIDIZED+ACTIVE+CENTER'>NFV</scene>, <scene name='pdbligand=S3F:OXIDIZED+[FE4-S3]+CLUSTER'>S3F</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4iub\|4iub]], [[4iud\|4iud]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4iub\|4iub]], [[4iud\|4iud]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hoxG, PHG002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16]), hoxK, PHG001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hoxG, PHG002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16]), hoxK, PHG001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Glucokinase Glucokinase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.~~2 2~~.7.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen:quinone_oxidoreductase Hydrogen:quinone oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.5.1 1.12.5.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iuc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iuc RCSB], [http://www.ebi.ac.uk/pdbsum/4iuc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iuc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iuc RCSB], [http://www.ebi.ac.uk/pdbsum/4iuc PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MBHL_CUPNH MBHL_CUPNH]] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions. [[http://www.uniprot.org/uniprot/MBHS_CUPNH MBHS_CUPNH]] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 17:
	Reversible [4Fe-3S] cluster morphing in an O2-tolerant [NiFe] hydrogenase.,Frielingsdorf S, Fritsch J, Schmidt A, Hammer M, Lowenstein J, Siebert E, Pelmenschikov V, Jaenicke T, Kalms J, Rippers Y, Lendzian F, Zebger I, Teutloff C, Kaupp M, Bittl R, Hildebrandt P, Friedrich B, Lenz O, Scheerer P Nat Chem Biol. 2014 May;10(5):378-85. doi: 10.1038/nchembio.1500. Epub 2014 Apr, 6. PMID:24705592<ref>PMID:24705592</ref>		Reversible [4Fe-3S] cluster morphing in an O2-tolerant [NiFe] hydrogenase.,Frielingsdorf S, Fritsch J, Schmidt A, Hammer M, Lowenstein J, Siebert E, Pelmenschikov V, Jaenicke T, Kalms J, Rippers Y, Lendzian F, Zebger I, Teutloff C, Kaupp M, Bittl R, Hildebrandt P, Friedrich B, Lenz O, Scheerer P Nat Chem Biol. 2014 May;10(5):378-85. doi: 10.1038/nchembio.1500. Epub 2014 Apr, 6. PMID:24705592<ref>PMID:24705592</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 23:		Line 25:
	[[Category: Alcaligenes eutropha h16]]		[[Category: Alcaligenes eutropha h16]]
	[[Category: Hydrogen:quinone oxidoreductase]]		[[Category: Hydrogen:quinone oxidoreductase]]
-	[[Category: Frielingsdorf, S.]]	+	[[Category: Frielingsdorf, S]]
-	[[Category: Fritsch, J.]]	+	[[Category: Fritsch, J]]
-	[[Category: Lenz, O.]]	+	[[Category: Lenz, O]]
-	[[Category: Scheerer, P.]]	+	[[Category: Scheerer, P]]
-	[[Category: Schmidt, A.]]	+	[[Category: Schmidt, A]]
	[[Category: Aerobic hydrogen bacteria]]		[[Category: Aerobic hydrogen bacteria]]
	[[Category: Bimetallic]]		[[Category: Bimetallic]]

OCA at 06:57, 14 May 2014

OCA — Wed, 14 May 2014 06:57:21 GMT

←Older revision		Revision as of 06:57, 14 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4iuc\| PDB=4iuc \| SCENE= }}~~	+	==Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 2==
-	===Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 2===	+	<StructureSection load='4iuc' size='340' side='right' caption='[[4iuc]], [[Resolution\|resolution]] 1.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4iuc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_eutropha_h16 Alcaligenes eutropha h16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUC OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NFV:NI-FE+OXIDIZED+ACTIVE+CENTER'>NFV</scene>, <scene name='pdbligand=S3F:OXIDIZED+[FE4-S3]+CLUSTER'>S3F</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4iub\|4iub]], [[4iud\|4iud]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hoxG, PHG002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16]), hoxK, PHG001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iuc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iuc RCSB], [http://www.ebi.ac.uk/pdbsum/4iuc PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hydrogenases catalyze the reversible oxidation of H2 into protons and electrons and are usually readily inactivated by O2. However, a subgroup of the [NiFe] hydrogenases, including the membrane-bound [NiFe] hydrogenase from Ralstonia eutropha, has evolved remarkable tolerance toward O2 that enables their host organisms to utilize H2 as an energy source at high O2. This feature is crucially based on a unique six cysteine-coordinated [4Fe-3S] cluster located close to the catalytic center, whose properties were investigated in this study using a multidisciplinary approach. The [4Fe-3S] cluster undergoes redox-dependent reversible transformations, namely iron swapping between a sulfide and a peptide amide N. Moreover, our investigations unraveled the redox-dependent and reversible occurence of an oxygen ligand located at a different iron. This ligand is hydrogen bonded to a conserved histidine that is essential for H2 oxidation at high O2. We propose that these transformations, reminiscent of those of the P-cluster of nitrogenase, enable the consecutive transfer of two electrons within a physiological potential range.

-	~~==Function==~~	+	Reversible [4Fe-3S] cluster morphing in an O2-tolerant [NiFe] hydrogenase.,Frielingsdorf S, Fritsch J, Schmidt A, Hammer M, Lowenstein J, Siebert E, Pelmenschikov V, Jaenicke T, Kalms J, Rippers Y, Lendzian F, Zebger I, Teutloff C, Kaupp M, Bittl R, Hildebrandt P, Friedrich B, Lenz O, Scheerer P Nat Chem Biol. 2014 May;10(5):378-85. doi: 10.1038/nchembio.1500. Epub 2014 Apr, 6. PMID:24705592<ref>PMID:24705592</ref>
-	[[~~http://www~~.~~uniprot~~.~~org/uniprot/MBHL_CUPNH MBHL_CUPNH]] This enzyme recycles the H~~(2) ~~produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon~~- ~~or phosphate-limited conditions~~. ~~[[http~~:/~~/www~~.~~uniprot~~.~~org~~/uniprot/MBHS_CUPNH MBHS_CUPNH]] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.	+

-	==~~About this Structure~~==	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	[[~~4iuc]] is a 2 chain structure. Full crystallographic information is available from [http~~:~~//oca.weizmann.ac.il/oca-bin/ocashort?id=4IUC OCA~~].	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Alcaligenes eutropha h16]]
	[[Category: Hydrogen:quinone oxidoreductase]]		[[Category: Hydrogen:quinone oxidoreductase]]
	[[Category: Frielingsdorf, S.]]		[[Category: Frielingsdorf, S.]]

OCA at 09:30, 2 April 2014

OCA — Wed, 02 Apr 2014 09:30:56 GMT

←Older revision		Revision as of 09:30, 2 April 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4iuc\| PDB=4iuc \| SCENE= }}
		+	===Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 2===

-	~~The entry 4iuc is ON HOLD until Apr 23 2015~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/MBHL_CUPNH MBHL_CUPNH]] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions. [[http://www.uniprot.org/uniprot/MBHS_CUPNH MBHS_CUPNH]] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.

-	~~Authors~~: Frielingsdorf, S.~~, Schmidt, A.,~~ Fritsch, J., Lenz, O., Scheerer, P.	+	==About this Structure==
-		+	[[4iuc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUC OCA].
-	~~Description~~: ~~Crystal structure of an O2~~-~~tolerant~~ [~~NiFe~~]-~~hydrogenase from Ralstonia eutropha in its as~~-~~isolated form~~ -~~oxidized~~ state 2	+	[[Category: Hydrogen:quinone oxidoreductase]]
		+	[[Category: Frielingsdorf, S.]]
		+	[[Category: Fritsch, J.]]
		+	[[Category: Lenz, O.]]
		+	[[Category: Scheerer, P.]]
		+	[[Category: Schmidt, A.]]
		+	[[Category: Aerobic hydrogen bacteria]]
		+	[[Category: Bimetallic]]
		+	[[Category: Hydrogen catalysis]]
		+	[[Category: Iron-sulfur cluster]]
		+	[[Category: Knallgas bacteria]]
		+	[[Category: Membrane-bound]]
		+	[[Category: Metalloenzyme]]
		+	[[Category: Metalloprotein catalytic center]]
		+	[[Category: Ni-fe active site]]
		+	[[Category: Nickel-iron cofactor]]
		+	[[Category: Oxidized state]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Oxygen-tolerant hydrogenase]]
		+	[[Category: Proteobacteria]]
		+	[[Category: Reduced state]]

OCA at 12:49, 11 December 2013

OCA — Wed, 11 Dec 2013 12:49:49 GMT

←Older revision		Revision as of 12:49, 11 December 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4iuc is ON HOLD until ~~Paper Publication~~	+	The entry 4iuc is ON HOLD until Apr 23 2015

	Authors: Frielingsdorf, S., Schmidt, A., Fritsch, J., Lenz, O., Scheerer, P.		Authors: Frielingsdorf, S., Schmidt, A., Fritsch, J., Lenz, O., Scheerer, P.

	Description: Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form -oxidized state 2		Description: Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form -oxidized state 2

OCA at 13:46, 6 March 2013

OCA — Wed, 06 Mar 2013 13:46:57 GMT

←Older revision		Revision as of 13:46, 6 March 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4iuc is ON HOLD	+	The entry 4iuc is ON HOLD until Paper Publication

	Authors: Frielingsdorf, S., Schmidt, A., Fritsch, J., Lenz, O., Scheerer, P.		Authors: Frielingsdorf, S., Schmidt, A., Fritsch, J., Lenz, O., Scheerer, P.

	Description: Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form -oxidized state 2		Description: Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form -oxidized state 2

OCA at 12:28, 6 February 2013

OCA — Wed, 06 Feb 2013 12:28:36 GMT

←Older revision		Revision as of 12:28, 6 February 2013
Line 3:		Line 3:
	The entry 4iuc is ON HOLD		The entry 4iuc is ON HOLD

-	Authors: Frielingsdorf, S. , Schmidt, A., Fritsch, J., Lenz, O., Scheerer, P.	+	Authors: Frielingsdorf, S., Schmidt, A., Fritsch, J., Lenz, O., Scheerer, P.

-	Description: Crystal structure of an O2-tolerant [NiFe]-~~Hydrogenase~~ from Ralstonia ~~Eutropha~~ in its as-isolated form -oxidized state 2	+	Description: Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form -oxidized state 2

OCA: Protected "4iuc" [edit=sysop:move=sysop]

OCA — Wed, 30 Jan 2013 12:04:58 GMT

Protected "4iuc" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:04, 30 January 2013