4j35 - Revision history

OCA at 21:29, 16 November 2022

OCA — Wed, 16 Nov 2022 21:29:23 GMT

←Older revision		Revision as of 21:29, 16 November 2022
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	<StructureSection load='4j35' size='340' side='right'caption='[[4j35]], [[Resolution\|resolution]] 1.78Å' scene=''>		<StructureSection load='4j35' size='340' side='right'caption='[[4j35]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4j35]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus~~ radiodurans~~" raj et al. 1960~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J35 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4J35 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4j35]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J35 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j35 OCA], [https://pdbe.org/4j35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j35 RCSB], [https://www.ebi.ac.uk/pdbsum/4j35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j35 ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4j2m\|4j2m]]~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4j35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j35 OCA], [~~http~~://pdbe.org/4j35 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4j35 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4j35 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4j35 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9RVU2_DEIRA Q9RVU2_DEIRA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Micrococcus~~ radiodurans ~~raj et al. 1960~~]]	+	[[Category: Deinococcus radiodurans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Barondeau, D]]	+	[[Category: Barondeau D]]
-	[[Category: Cker~~, B H~~]]	+	[[Category: Cker BH]]
-	[[Category: Fox, N]]	+	[[Category: Fox N]]
-	[[Category: Malisi, C]]	+	[[Category: Malisi C]]
-	[[Category: Meier, M]]	+	[[Category: Meier M]]
-	[[Category: Rajendran, C]]	+	[[Category: Rajendran C]]
-	[[Category: Raushel, F]]	+	[[Category: Raushel F]]
-	[[Category: Schlee, S]]	+	[[Category: Schlee S]]
-	[[Category: Sterner, R]]	+	[[Category: Sterner R]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Molecular engineering]]~~	+
-	~~[[Category: Organophosphate hydrolysis activity~~]]	+

OCA at 09:28, 3 April 2019

OCA — Wed, 03 Apr 2019 09:28:26 GMT

←Older revision		Revision as of 09:28, 3 April 2019
Line 1:		Line 1:

	==Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template==		==Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template==
-	<StructureSection load='4j35' size='340' side='right' caption='[[4j35]], [[Resolution\|resolution]] 1.78Å' scene=''>	+	<StructureSection load='4j35' size='340' side='right'caption='[[4j35]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4j35]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J35 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J35 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4j35]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J35 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J35 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Micrococcus radiodurans raj et al. 1960]]		[[Category: Micrococcus radiodurans raj et al. 1960]]
		+	[[Category: Large Structures]]
	[[Category: Barondeau, D]]		[[Category: Barondeau, D]]
	[[Category: Cker, B H]]		[[Category: Cker, B H]]

OCA at 16:29, 4 August 2016

OCA — Thu, 04 Aug 2016 16:29:18 GMT

←Older revision		Revision as of 16:29, 4 August 2016
Line 1:		Line 1:
		+
	==Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template==		==Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template==
	<StructureSection load='4j35' size='340' side='right' caption='[[4j35]], [[Resolution\|resolution]] 1.78Å' scene=''>		<StructureSection load='4j35' size='340' side='right' caption='[[4j35]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4j35]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Deinococcus_radiodurans Deinococcus~~ radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J35 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J35 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4j35]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J35 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J35 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4j2m\|4j2m]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4j2m\|4j2m]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j35 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j35 RCSB], [http://www.ebi.ac.uk/pdbsum/4j35 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j35 OCA], [http://pdbe.org/4j35 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j35 RCSB], [http://www.ebi.ac.uk/pdbsum/4j35 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j35 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4j35" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Deinococcus~~ radiodurans]]	+	[[Category: Micrococcus radiodurans raj et al. 1960]]
	[[Category: Barondeau, D]]		[[Category: Barondeau, D]]
	[[Category: Cker, B H]]		[[Category: Cker, B H]]

OCA at 12:56, 21 December 2014

OCA — Sun, 21 Dec 2014 12:56:10 GMT

←Older revision		Revision as of 12:56, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4j35\| PDB=4j35 \| SCENE= }}~~	+	==Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template==
-	===Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template===	+	<StructureSection load='4j35' size='340' side='right' caption='[[4j35]], [[Resolution\|resolution]] 1.78Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23837603}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4j35]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J35 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J35 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4j2m\|4j2m]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j35 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j35 RCSB], [http://www.ebi.ac.uk/pdbsum/4j35 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rapid evolution of enzymes provides unique molecular insights into the remarkable adaptability of proteins and helps to elucidate the relationship between amino acid sequence, structure and function. We interrogated the evolution of the phosphotriesterase from Pseudomonas diminuta (PdPTE), which hydrolyzes synthetic organophosphates with remarkable catalytic efficiency. PTE is thought to be an evolutionarily "young" enzyme and it has been postulated that it has evolved from members of the phosphotriesterase-like lactonase (PLL) family that show promiscuous organophosphate degrading activity. Starting from a weakly promiscuous PLL scaffold (Dr0930 from Deinococcus radiodurans), we designed an extremely efficient organophosphate hydrolase (OPH) with broad substrate specificity using rational and random mutagenesis in combination with in vitro activity screening. The OPH activity for seven organophosphate substrates was simultaneously enhanced by up to five orders of magnitude, achieving absolute values of catalytic efficiencies up to 106 M-1 s-1. Structural and computational analyses identified the molecular basis for the enhanced OPH activity of the engineered PLL variants and demonstrated that OPH catalysis in PdPTE and the engineered PLL differ significantly in the mode of substrate binding.

-	~~==About this Structure==~~	+	Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template.,Meier MM, Rajendran C, Malisi C, Fox NG, Xu C, Schlee S, Barondeau DP, Hoecker B, Sterner R, Raushel FM J Am Chem Soc. 2013 Jul 9. PMID:23837603<ref>PMID:23837603</ref>
-	~~[[4j35]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4J35 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023837603</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Deinococcus radiodurans]]		[[Category: Deinococcus radiodurans]]
-	[[Category: Barondeau, D.]]	+	[[Category: Barondeau, D]]
-	[[Category: Cker, B H.]]	+	[[Category: Cker, B H]]
-	[[Category: Fox, N.]]	+	[[Category: Fox, N]]
-	[[Category: Malisi, C.]]	+	[[Category: Malisi, C]]
-	[[Category: Meier, M.]]	+	[[Category: Meier, M]]
-	[[Category: Rajendran, C.]]	+	[[Category: Rajendran, C]]
-	[[Category: Raushel, F.]]	+	[[Category: Raushel, F]]
-	[[Category: Schlee, S.]]	+	[[Category: Schlee, S]]
-	[[Category: Sterner, R.]]	+	[[Category: Sterner, R]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Molecular engineering]]		[[Category: Molecular engineering]]
	[[Category: Organophosphate hydrolysis activity]]		[[Category: Organophosphate hydrolysis activity]]

OCA at 12:59, 24 July 2013

OCA — Wed, 24 Jul 2013 12:59:10 GMT

←Older revision		Revision as of 12:59, 24 July 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4j35\| PDB=4j35 \| SCENE= }}
		+	===Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template===
		+	{{ABSTRACT_PUBMED_23837603}}

-	~~The entry~~ 4j35 is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4j35]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J35 OCA].

-	~~Authors~~: ~~Sterner~~, R., ~~Raushel~~, F., Meier, M., Rajendran, C., ~~Malisi, C~~.~~, Fox, N.,~~ Schlee, S., ~~Barondeau, D., Cker, B.H~~.	+	==Reference==
-		+	<ref group="xtra">PMID:023837603</ref><references group="xtra"/><references/>
-	~~Description~~: Molecular ~~Engineering of~~ Organophosphate ~~Hydrolysis Activity from a Weak Promiscuous Lactonase Template~~	+	[[Category: Deinococcus radiodurans]]
		+	[[Category: Barondeau, D.]]
		+	[[Category: Cker, B H.]]
		+	[[Category: Fox, N.]]
		+	[[Category: Malisi, C.]]
		+	[[Category: Meier, M.]]
		+	[[Category: Rajendran, C.]]
		+	[[Category: Raushel, F.]]
		+	[[Category: Schlee, S.]]
		+	[[Category: Sterner, R.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Molecular engineering]]
		+	[[Category: Organophosphate hydrolysis activity]]

OCA at 08:14, 27 March 2013

OCA — Wed, 27 Mar 2013 08:14:52 GMT

←Older revision		Revision as of 08:14, 27 March 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4j35 is ON HOLD	+	The entry 4j35 is ON HOLD until Paper Publication

	Authors: Sterner, R., Raushel, F., Meier, M., Rajendran, C., Malisi, C., Fox, N., Schlee, S., Barondeau, D., Cker, B.H.		Authors: Sterner, R., Raushel, F., Meier, M., Rajendran, C., Malisi, C., Fox, N., Schlee, S., Barondeau, D., Cker, B.H.

	Description: Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template		Description: Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template

OCA at 13:29, 20 February 2013

OCA — Wed, 20 Feb 2013 13:29:28 GMT

←Older revision		Revision as of 13:29, 20 February 2013
Line 3:		Line 3:
	The entry 4j35 is ON HOLD		The entry 4j35 is ON HOLD

-	Authors: ~~Reinhard~~ Sterner, ~~Frank~~ Raushel, ~~Monika~~ Meier, ~~Chitra~~ Rajendran, ~~Christoph~~ Malisi, ~~Nicholas~~ Fox, ~~Sandra~~ Schlee, ~~David~~ Barondeau , ~~Birte~~ H ~~cker~~	+	Authors: Sterner, R., Raushel, F., Meier, M., Rajendran, C., Malisi, C., Fox, N., Schlee, S., Barondeau, D., Cker, B.H.

	Description: Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template		Description: Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template

OCA: Protected "4j35" [edit=sysop:move=sysop]

OCA — Wed, 13 Feb 2013 10:11:03 GMT

Protected "4j35" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:11, 13 February 2013

OCA: New page: '''Unreleased structure''' The entry 4j35 is ON HOLD Authors: Reinhard Sterner, Frank Raushel, Monika Meier, Chitra Rajendran, Christoph Malisi, Nicholas Fox, Sandra Schlee, David Baron...

OCA — Wed, 13 Feb 2013 10:11:01 GMT

New page: '''Unreleased structure''' The entry 4j35 is ON HOLD Authors: Reinhard Sterner, Frank Raushel, Monika Meier, Chitra Rajendran, Christoph Malisi, Nicholas Fox, Sandra Schlee, David Baron...

New page

'''Unreleased structure'''

The entry 4j35 is ON HOLD

Authors: Reinhard Sterner, Frank Raushel, Monika Meier, Chitra Rajendran, Christoph Malisi, Nicholas Fox, Sandra Schlee, David Barondeau , Birte H cker

Description: Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template