4j5t - Revision history

OCA at 21:34, 16 November 2022

OCA — Wed, 16 Nov 2022 21:34:11 GMT

←Older revision		Revision as of 21:34, 16 November 2022
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	==Crystal structure of Processing alpha-Glucosidase I==		==Crystal structure of Processing alpha-Glucosidase I==
-	<StructureSection load='4j5t' size='340' side='right' caption='[[4j5t]], [[Resolution\|resolution]] 2.04Å' scene=''>	+	<StructureSection load='4j5t' size='340' side='right'caption='[[4j5t]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4j5t]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4J5T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4j5t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J5T FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CWH41, GLS1, YGL027C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5t OCA], [https://pdbe.org/4j5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j5t RCSB], [https://www.ebi.ac.uk/pdbsum/4j5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j5t ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannosyl-oligosaccharide_glucosidase Mannosyl-oligosaccharide glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.106 3.2.1.106] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4j5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5t OCA], [~~http~~://pdbe.org/4j5t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4j5t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4j5t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4j5t ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CWH41_YEAST CWH41_YEAST]] Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor highly specifically. Seems to play a role in beta-1,6-glucan synthesis.	+	[https://www.uniprot.org/uniprot/CWH41_YEAST CWH41_YEAST] Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor highly specifically. Seems to play a role in beta-1,6-glucan synthesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Baker's yeast~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Mannosyl-oligosaccharide glucosidase~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Barker~~, M K~~]]	+	[[Category: Barker MK]]
-	[[Category: Rose~~, D R]]~~	+	[[Category: Rose DR]]
-	~~[[Category: Er membrane]]~~	+
-	~~[[Category: Glycoside hydrolase]]~~	+
-	~~[[Category: Glycosylation]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: N-glycoprotein~~]]	+

OCA at 20:41, 5 August 2016

OCA — Fri, 05 Aug 2016 20:41:01 GMT

←Older revision		Revision as of 20:41, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Processing alpha-Glucosidase I==		==Crystal structure of Processing alpha-Glucosidase I==
	<StructureSection load='4j5t' size='340' side='right' caption='[[4j5t]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='4j5t' size='340' side='right' caption='[[4j5t]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4j5t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J5T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4j5t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J5T FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CWH41, GLS1, YGL027C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 ~~Saccharomyces cerevisiae S288c~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CWH41, GLS1, YGL027C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannosyl-oligosaccharide_glucosidase Mannosyl-oligosaccharide glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.106 3.2.1.106] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannosyl-oligosaccharide_glucosidase Mannosyl-oligosaccharide glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.106 3.2.1.106] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j5t RCSB], [http://www.ebi.ac.uk/pdbsum/4j5t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5t OCA], [http://pdbe.org/4j5t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j5t RCSB], [http://www.ebi.ac.uk/pdbsum/4j5t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j5t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4j5t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
	[[Category: Mannosyl-oligosaccharide glucosidase]]		[[Category: Mannosyl-oligosaccharide glucosidase]]
-	[[Category: Saccharomyces cerevisiae s288c]]
	[[Category: Barker, M K]]		[[Category: Barker, M K]]
	[[Category: Rose, D R]]		[[Category: Rose, D R]]

OCA at 05:42, 25 December 2014

OCA — Thu, 25 Dec 2014 05:42:21 GMT

←Older revision		Revision as of 05:42, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j5t RCSB], [http://www.ebi.ac.uk/pdbsum/4j5t PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j5t RCSB], [http://www.ebi.ac.uk/pdbsum/4j5t PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CWH41_YEAST CWH41_YEAST]] Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor highly specifically. Seems to play a role in beta-1,6-glucan synthesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 13:00, 21 December 2014

OCA — Sun, 21 Dec 2014 13:00:43 GMT

←Older revision		Revision as of 13:00, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4j5t\| PDB=4j5t \| SCENE= }}~~	+	==Crystal structure of Processing alpha-Glucosidase I==
-	===Crystal structure of Processing alpha-Glucosidase I===	+	<StructureSection load='4j5t' size='340' side='right' caption='[[4j5t]], [[Resolution\|resolution]] 2.04Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23536181}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4j5t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J5T FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CWH41, GLS1, YGL027C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannosyl-oligosaccharide_glucosidase Mannosyl-oligosaccharide glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.106 3.2.1.106] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j5t RCSB], [http://www.ebi.ac.uk/pdbsum/4j5t PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Processing alpha-glucosidase I (GluI) is a key member of the eukaryotic N-glycosylation processing pathway, selectively catalyzing the first glycoprotein trimming step in the endoplasmic reticulum. Inhibition of GluI activity impacts the infectivity of enveloped viruses; however, despite interest in this protein from a structural, enzymatic, and therapeutic standpoint, little is known about its structure and enzymatic mechanism in catalysis of the unique glycan substrate Glc3Man9GlcNAc2. The first structural model of eukaryotic GluI is here presented at 2-A resolution. Two catalytic residues are proposed, mutations of which result in catalytically inactive, properly folded protein. Using Autodocking methods with the known substrate and inhibitors as ligands, including a novel inhibitor characterized in this work, the active site of GluI was mapped. From these results, a model of substrate binding has been formulated, which is most likely conserved in mammalian GluI.

-	~~==Function==~~	+	Specificity of Processing alpha-Glucosidase I Is Guided by the Substrate Conformation: CRYSTALLOGRAPHIC AND IN SILICO STUDIES.,Barker MK, Rose DR J Biol Chem. 2013 May 10;288(19):13563-74. doi: 10.1074/jbc.M113.460436. Epub, 2013 Mar 27. PMID:23536181<ref>PMID:23536181</ref>
-	~~[[http://www.uniprot.org/uniprot/CWH41_YEAST CWH41_YEAST]] Cleaves the distal~~ alpha ~~1,2~~-~~linked glucose residue from~~ the ~~Glc~~(3)~~Man(9)GlcNAc(2) oligosaccharide precursor highly specifically. Seems to play a role in beta~~-1,~~6-glucan synthesis~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4j5t]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4J5T OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023536181</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Mannosyl-oligosaccharide glucosidase]]		[[Category: Mannosyl-oligosaccharide glucosidase]]
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]
-	[[Category: Barker, M K.]]	+	[[Category: Barker, M K]]
-	[[Category: Rose, D R.]]	+	[[Category: Rose, D R]]
	[[Category: Er membrane]]		[[Category: Er membrane]]
	[[Category: Glycoside hydrolase]]		[[Category: Glycoside hydrolase]]

OCA at 09:17, 19 June 2013

OCA — Wed, 19 Jun 2013 09:17:02 GMT

←Older revision		Revision as of 09:17, 19 June 2013
Line 1:		Line 1:
	{{STRUCTURE_4j5t\| PDB=4j5t \| SCENE= }}		{{STRUCTURE_4j5t\| PDB=4j5t \| SCENE= }}
	===Crystal structure of Processing alpha-Glucosidase I===		===Crystal structure of Processing alpha-Glucosidase I===
		+	{{ABSTRACT_PUBMED_23536181}}

	==Function==		==Function==
Line 7:		Line 8:
	==About this Structure==		==About this Structure==
	[[4j5t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5T OCA].		[[4j5t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5T OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023536181</ref><references group="xtra"/><references/>
	[[Category: Mannosyl-oligosaccharide glucosidase]]		[[Category: Mannosyl-oligosaccharide glucosidase]]
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]

OCA at 01:53, 4 April 2013

OCA — Thu, 04 Apr 2013 01:53:07 GMT

←Older revision		Revision as of 01:53, 4 April 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4j5t\| PDB=4j5t \| SCENE= }}
		+	===Crystal structure of Processing alpha-Glucosidase I===

-	~~The entry 4j5t is ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/CWH41_YEAST CWH41_YEAST]] Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor highly specifically. Seems to play a role in beta-1,6-glucan synthesis.

-	~~Authors~~: Barker, M.K., Rose, D.R.	+	==About this Structure==
-		+	[[4j5t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5T OCA].
-	~~Description~~: ~~Crystal structure of Processing alpha~~-~~Glucosidase I~~	+	[[Category: Mannosyl-oligosaccharide glucosidase]]
		+	[[Category: Saccharomyces cerevisiae s288c]]
		+	[[Category: Barker, M K.]]
		+	[[Category: Rose, D R.]]
		+	[[Category: Er membrane]]
		+	[[Category: Glycoside hydrolase]]
		+	[[Category: Glycosylation]]
		+	[[Category: Hydrolase]]
		+	[[Category: N-glycoprotein]]

OCA at 16:16, 20 March 2013

OCA — Wed, 20 Mar 2013 16:16:21 GMT

←Older revision		Revision as of 16:16, 20 March 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4j5t is ON HOLD	+	The entry 4j5t is ON HOLD until Paper Publication

	Authors: Barker, M.K., Rose, D.R.		Authors: Barker, M.K., Rose, D.R.

	Description: Crystal structure of Processing alpha-Glucosidase I		Description: Crystal structure of Processing alpha-Glucosidase I

OCA: Protected "4j5t" [edit=sysop:move=sysop]

OCA — Wed, 20 Feb 2013 13:31:17 GMT

Protected "4j5t" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:31, 20 February 2013

OCA: New page: '''Unreleased structure''' The entry 4j5t is ON HOLD Authors: Barker, M.K., Rose, D.R. Description: Crystal structure of Processing alpha-Glucosidase I

OCA — Wed, 20 Feb 2013 13:31:16 GMT

New page: '''Unreleased structure''' The entry 4j5t is ON HOLD Authors: Barker, M.K., Rose, D.R. Description: Crystal structure of Processing alpha-Glucosidase I

New page

'''Unreleased structure'''

The entry 4j5t is ON HOLD

Authors: Barker, M.K., Rose, D.R.

Description: Crystal structure of Processing alpha-Glucosidase I