4jce - Revision history

OCA at 15:40, 20 September 2023

OCA — Wed, 20 Sep 2023 15:40:12 GMT

←Older revision		Revision as of 15:40, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4jce]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/JC_polyomavirus JC polyomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JCE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4jce]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/JC_polyomavirus JC polyomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JCE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jce OCA], [https://pdbe.org/4jce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jce RCSB], [https://www.ebi.ac.uk/pdbsum/4jce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jce ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jce OCA], [https://pdbe.org/4jce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jce RCSB], [https://www.ebi.ac.uk/pdbsum/4jce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jce ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:04, 24 November 2022

OCA — Thu, 24 Nov 2022 11:04:48 GMT

←Older revision		Revision as of 11:04, 24 November 2022
Line 3:		Line 3:
	<StructureSection load='4jce' size='340' side='right'caption='[[4jce]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4jce' size='340' side='right'caption='[[4jce]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4jce]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human_polyomavirus_(type_jc) Human~~ polyomavirus ~~(type jc)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JCE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4JCE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4jce]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/JC_polyomavirus JC polyomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JCE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nxd\|3nxd]], [[3nxg\|3nxg]], [[4jcd\|4jcd]], [[4jcf\|4jcf]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jce OCA], [https://pdbe.org/4jce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jce RCSB], [https://www.ebi.ac.uk/pdbsum/4jce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jce ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4jce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jce OCA], [~~http~~://pdbe.org/4jce PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4jce RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4jce PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4jce ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/VP1_POVJC VP1_POVJC]] Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with a N-linked glycoprotein containing terminal alpha(2-6)-linked sialic acids on the cell surface to provide virion attachment to target cell. The serotonergic receptor 5HT2AR also acts as a cellular receptor for JCV on human glial cells. Once attached, the virions enter predominantly by a ligand-inducible clathrin-dependent pathway and traffic to the ER. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA at nuclear domains called promyelocytic leukemia (PML) bodies, and participates in rearranging nucleosomes around the viral DNA.<ref>PMID:10666259</ref>	+	[https://www.uniprot.org/uniprot/VP1_POVJC VP1_POVJC] Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with a N-linked glycoprotein containing terminal alpha(2-6)-linked sialic acids on the cell surface to provide virion attachment to target cell. The serotonergic receptor 5HT2AR also acts as a cellular receptor for JCV on human glial cells. Once attached, the virions enter predominantly by a ligand-inducible clathrin-dependent pathway and traffic to the ER. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA at nuclear domains called promyelocytic leukemia (PML) bodies, and participates in rearranging nucleosomes around the viral DNA.<ref>PMID:10666259</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4jce" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4jce" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Virus coat proteins 3D structures\|Virus coat proteins 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: JC polyomavirus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Stehle, T]]	+	[[Category: Stehle T]]
-	[[Category: Stroh~~, L J]]~~	+	[[Category: Stroh LJ]]
-	~~[[Category: Beta-sandwich]]~~	+
-	~~[[Category: Jelly roll topology]]~~	+
-	~~[[Category: Major capsid protein]]~~	+
-	~~[[Category: Pml-associated vp1 mutation]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 08:44, 3 April 2019

OCA — Wed, 03 Apr 2019 08:44:53 GMT

←Older revision		Revision as of 08:44, 3 April 2019
Line 1:		Line 1:

	==L54F Variant of JC Polyomavirus Major Capsid Protein VP1==		==L54F Variant of JC Polyomavirus Major Capsid Protein VP1==
-	<StructureSection load='4jce' size='340' side='right' caption='[[4jce]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='4jce' size='340' side='right'caption='[[4jce]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4jce]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_polyomavirus_(type_jc) Human polyomavirus (type jc)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JCE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4jce]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_polyomavirus_(type_jc) Human polyomavirus (type jc)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JCE FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]
	[[Category: Stroh, L J]]		[[Category: Stroh, L J]]

OCA at 01:27, 5 August 2016

OCA — Fri, 05 Aug 2016 01:27:49 GMT

←Older revision		Revision as of 01:27, 5 August 2016
Line 1:		Line 1:
		+
	==L54F Variant of JC Polyomavirus Major Capsid Protein VP1==		==L54F Variant of JC Polyomavirus Major Capsid Protein VP1==
	<StructureSection load='4jce' size='340' side='right' caption='[[4jce]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4jce' size='340' side='right' caption='[[4jce]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4jce]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Jc_polyomavirus Jc~~ polyomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JCE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4jce]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_polyomavirus_(type_jc) Human polyomavirus (type jc)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JCE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nxd\|3nxd]], [[3nxg\|3nxg]], [[4jcd\|4jcd]], [[4jcf\|4jcf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nxd\|3nxd]], [[3nxg\|3nxg]], [[4jcd\|4jcd]], [[4jcf\|4jcf]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jce OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jce RCSB], [http://www.ebi.ac.uk/pdbsum/4jce PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jce OCA], [http://pdbe.org/4jce PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jce RCSB], [http://www.ebi.ac.uk/pdbsum/4jce PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jce ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4jce" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Jc polyomavirus]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]
	[[Category: Stroh, L J]]		[[Category: Stroh, L J]]

OCA at 21:36, 24 December 2014

OCA — Wed, 24 Dec 2014 21:36:23 GMT

←Older revision		Revision as of 21:36, 24 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4jce\| PDB=4jce \| SCENE= }}~~	+	==L54F Variant of JC Polyomavirus Major Capsid Protein VP1==
-	===L54F Variant of JC Polyomavirus Major Capsid Protein VP1===	+	<StructureSection load='4jce' size='340' side='right' caption='[[4jce]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23760462}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4jce]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Jc_polyomavirus Jc polyomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JCE FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nxd\|3nxd]], [[3nxg\|3nxg]], [[4jcd\|4jcd]], [[4jcf\|4jcf]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jce OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jce RCSB], [http://www.ebi.ac.uk/pdbsum/4jce PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/VP1_POVJC VP1_POVJC]] Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with a N-linked glycoprotein containing terminal alpha(2-6)-linked sialic acids on the cell surface to provide virion attachment to target cell. The serotonergic receptor 5HT2AR also acts as a cellular receptor for JCV on human glial cells. Once attached, the virions enter predominantly by a ligand-inducible clathrin-dependent pathway and traffic to the ER. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA at nuclear domains called promyelocytic leukemia (PML) bodies, and participates in rearranging nucleosomes around the viral DNA.<ref>PMID:10666259</ref>		[[http://www.uniprot.org/uniprot/VP1_POVJC VP1_POVJC]] Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with a N-linked glycoprotein containing terminal alpha(2-6)-linked sialic acids on the cell surface to provide virion attachment to target cell. The serotonergic receptor 5HT2AR also acts as a cellular receptor for JCV on human glial cells. Once attached, the virions enter predominantly by a ligand-inducible clathrin-dependent pathway and traffic to the ER. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA at nuclear domains called promyelocytic leukemia (PML) bodies, and participates in rearranging nucleosomes around the viral DNA.<ref>PMID:10666259</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The human JC polyomavirus (JCPyV) is the causative agent of the fatal, demyelinating disease progressive multifocal leukoencephalopathy (PML). The Mad-1 prototype strain of JCPyV uses the glycan lactoseries tetrasaccharide c (LSTc) and serotonin receptor 5-HT2A to attach to and enter into host cells, respectively. Specific residues in the viral capsid protein VP1 are responsible for direct interactions with the alpha2,6-linked sialic acid of LSTc. Viral isolates from individuals with PML often contain mutations in the sialic acid-binding pocket of VP1 that are hypothesized to arise from positive selection. We reconstituted these mutations in the Mad-1 strain of JCPyV and found that they were not capable of growth. The mutations were then introduced into recombinant VP1 and reconstituted as pentamers in order to conduct binding studies and structural analyses. VP1 pentamers carrying PML-associated mutations were not capable of binding to permissive cells. High-resolution structure determination revealed that these pentamers are well folded but no longer bind to LSTc due to steric clashes in the sialic acid-binding site. Reconstitution of the mutations into JCPyV pseudoviruses allowed us to directly quantify the infectivity of the mutants in several cell lines. The JCPyV pseudoviruses with PML-associated mutations were not infectious, nor were they able to engage sialic acid as measured by hemagglutination of human red blood cells. These results demonstrate that viruses from PML patients with single point mutations in VP1 disrupt binding to sialic acid motifs and render these viruses noninfectious. IMPORTANCE Infection with human JC polyomavirus (JCPyV) is common and asymptomatic in healthy individuals, but during immunosuppression, JCPyV can spread from the kidney to the central nervous system (CNS) and cause a fatal, demyelinating disease, progressive multifocal leukoencephalopathy (PML). Individuals infected with HIV, those who have AIDS, or those receiving immunomodulatory therapies for autoimmune diseases are at serious risk for PML. Recent reports have demonstrated that viral isolates from PML patients often have distinct changes within the major capsid protein. Our structural-functional approach highlights that these mutations result in abolished engagement of the carbohydrate receptor motif LSTc that is necessary for infection. Viruses with PML-associated mutations are not infectious in glial cells, suggesting that they may play an alternative role in PML pathogenesis.

-	~~==About this Structure==~~	+	Progressive multifocal leukoencephalopathy-associated mutations in the JC polyomavirus capsid disrupt lactoseries tetrasaccharide c binding.,Maginnis MS, Stroh LJ, Gee GV, O'Hara BA, Derdowski A, Stehle T, Atwood WJ MBio. 2013 Jun 11;4(3):e00247-13. doi: 10.1128/mBio.00247-13. PMID:23760462<ref>PMID:23760462</ref>
-	~~[[4jce]] is a 5 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Jc_polyomavirus Jc polyomavirus]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4JCE OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023760462</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Jc polyomavirus]]		[[Category: Jc polyomavirus]]
-	[[Category: Stehle, T.]]	+	[[Category: Stehle, T]]
-	[[Category: Stroh, L J.]]	+	[[Category: Stroh, L J]]
	[[Category: Beta-sandwich]]		[[Category: Beta-sandwich]]
	[[Category: Jelly roll topology]]		[[Category: Jelly roll topology]]

OCA at 07:54, 14 August 2013

OCA — Wed, 14 Aug 2013 07:54:09 GMT

←Older revision		Revision as of 07:54, 14 August 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4jce\| PDB=4jce \| SCENE= }}
		+	===L54F Variant of JC Polyomavirus Major Capsid Protein VP1===
		+	{{ABSTRACT_PUBMED_23760462}}

-	The ~~entry 4jce~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/VP1_POVJC VP1_POVJC]] Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with a N-linked glycoprotein containing terminal alpha(2-6)-linked sialic acids on the cell surface to provide virion attachment to target cell. The serotonergic receptor 5HT2AR also acts as a cellular receptor for JCV on human glial cells. Once attached, the virions enter predominantly by a ligand-inducible clathrin-dependent pathway and traffic to the ER. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA at nuclear domains called promyelocytic leukemia (PML) bodies, and participates in rearranging nucleosomes around the viral DNA.<ref>PMID:10666259</ref>

-	~~Authors~~: ~~Stehle, T~~.~~, Stroh, L~~.J.	+	==About this Structure==
		+	[[4jce]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Jc_polyomavirus Jc polyomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JCE OCA].

-	~~Description~~: ~~L54F Variant of JC Polyomavirus~~ Major ~~Capsid Protein VP1~~	+	==Reference==
		+	<ref group="xtra">PMID:023760462</ref><references group="xtra"/><references/>
		+	[[Category: Jc polyomavirus]]
		+	[[Category: Stehle, T.]]
		+	[[Category: Stroh, L J.]]
		+	[[Category: Beta-sandwich]]
		+	[[Category: Jelly roll topology]]
		+	[[Category: Major capsid protein]]
		+	[[Category: Pml-associated vp1 mutation]]
		+	[[Category: Viral protein]]

OCA at 19:52, 17 April 2013

OCA — Wed, 17 Apr 2013 19:52:58 GMT

←Older revision		Revision as of 19:52, 17 April 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4jce is ON HOLD	+	The entry 4jce is ON HOLD until Paper Publication

	Authors: Stehle, T., Stroh, L.J.		Authors: Stehle, T., Stroh, L.J.

	Description: L54F Variant of JC Polyomavirus Major Capsid Protein VP1		Description: L54F Variant of JC Polyomavirus Major Capsid Protein VP1

OCA: Protected "4jce" [edit=sysop:move=sysop]

OCA — Wed, 27 Feb 2013 14:29:29 GMT

Protected "4jce" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:29, 27 February 2013

OCA: New page: '''Unreleased structure''' The entry 4jce is ON HOLD Authors: Stehle, T., Stroh, L.J. Description: L54F Variant of JC Polyomavirus Major Capsid Protein VP1

OCA — Wed, 27 Feb 2013 14:29:28 GMT

New page: '''Unreleased structure''' The entry 4jce is ON HOLD Authors: Stehle, T., Stroh, L.J. Description: L54F Variant of JC Polyomavirus Major Capsid Protein VP1

New page

'''Unreleased structure'''

The entry 4jce is ON HOLD

Authors: Stehle, T., Stroh, L.J.

Description: L54F Variant of JC Polyomavirus Major Capsid Protein VP1