4jfz - Revision history

OCA at 11:11, 24 November 2022

OCA — Thu, 24 Nov 2022 11:11:17 GMT

←Older revision		Revision as of 11:11, 24 November 2022
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	<StructureSection load='4jfz' size='340' side='right'caption='[[4jfz]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='4jfz' size='340' side='right'caption='[[4jfz]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4jfz]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JFZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4JFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4jfz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JFZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPI:PROPANOIC+ACID'>PPI</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PPI:PROPANOIC+ACID'>PPI</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jfz OCA], [https://pdbe.org/4jfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jfz RCSB], [https://www.ebi.ac.uk/pdbsum/4jfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jfz ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4jfx\|4jfx]], [[4jfy\|4jfy]], [[4jg0\|4jg0]], [[4jg1\|4jg1]]~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4jfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jfz OCA], [~~http~~://pdbe.org/4jfz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4jfz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4jfz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4jfz ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Degrado~~, W F~~]]	+	[[Category: Degrado WF]]
-	[[Category: Hannigan~~, B T~~]]	+	[[Category: Hannigan BT]]
-	[[Category: Koerber~~, J T~~]]	+	[[Category: Koerber JT]]
-	[[Category: Thomsen~~, N D~~]]	+	[[Category: Thomsen ND]]
-	[[Category: Wells~~, J A]]~~	+	[[Category: Wells JA]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin domain~~]]	+

OCA at 05:04, 13 February 2020

OCA — Thu, 13 Feb 2020 05:04:55 GMT

←Older revision		Revision as of 05:04, 13 February 2020
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	==Structure of phosphoserine (pSAb) scaffold bound to pSer peptide==		==Structure of phosphoserine (pSAb) scaffold bound to pSer peptide==
-	<StructureSection load='4jfz' size='340' side='right' caption='[[4jfz]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='4jfz' size='340' side='right'caption='[[4jfz]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4jfz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JFZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4jfz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JFZ FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Degrado, W F]]		[[Category: Degrado, W F]]
	[[Category: Hannigan, B T]]		[[Category: Hannigan, B T]]

OCA at 10:01, 11 August 2016

OCA — Thu, 11 Aug 2016 10:01:21 GMT

←Older revision		Revision as of 10:01, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of phosphoserine (pSAb) scaffold bound to pSer peptide==		==Structure of phosphoserine (pSAb) scaffold bound to pSer peptide==
	<StructureSection load='4jfz' size='340' side='right' caption='[[4jfz]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='4jfz' size='340' side='right' caption='[[4jfz]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4jfz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus coli" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4jfz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JFZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPI:PROPANOIC+ACID'>PPI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPI:PROPANOIC+ACID'>PPI</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4jfx\|4jfx]], [[4jfy\|4jfy]], [[4jg0\|4jg0]], [[4jg1\|4jg1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4jfx\|4jfx]], [[4jfy\|4jfy]], [[4jg0\|4jg0]], [[4jg1\|4jg1]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jfz RCSB], [http://www.ebi.ac.uk/pdbsum/4jfz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jfz OCA], [http://pdbe.org/4jfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jfz RCSB], [http://www.ebi.ac.uk/pdbsum/4jfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jfz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4jfz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus coli migula 1895~~]]	+	[[Category: Human]]
	[[Category: Degrado, W F]]		[[Category: Degrado, W F]]
	[[Category: Hannigan, B T]]		[[Category: Hannigan, B T]]

OCA at 13:46, 4 January 2015

OCA — Sun, 04 Jan 2015 13:46:23 GMT

←Older revision		Revision as of 13:46, 4 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4jfz\| PDB=4jfz \| SCENE= }}~~	+	==Structure of phosphoserine (pSAb) scaffold bound to pSer peptide==
-	===Structure of phosphoserine (pSAb) scaffold bound to pSer peptide===	+	<StructureSection load='4jfz' size='340' side='right' caption='[[4jfz]], [[Resolution\|resolution]] 1.75Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23955275}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4jfz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JFZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPI:PROPANOIC+ACID'>PPI</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4jfx\|4jfx]], [[4jfy\|4jfy]], [[4jg0\|4jg0]], [[4jg1\|4jg1]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jfz RCSB], [http://www.ebi.ac.uk/pdbsum/4jfz PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aberrant changes in post-translational modifications (PTMs) such as phosphate groups underlie a majority of human diseases. However, detection and quantification of PTMs for diagnostic or biomarker applications often require PTM-specific monoclonal antibodies (mAbs), which are challenging to generate using traditional antibody-selection methods. Here we outline a general strategy for producing synthetic, PTM-specific mAbs by engineering a motif-specific 'hot spot' into an antibody scaffold. Inspired by a natural phosphate-binding motif, we designed and selected mAb scaffolds with hot spots specific for phosphoserine, phosphothreonine or phosphotyrosine. Crystal structures of the phospho-specific mAbs revealed two distinct modes of phosphoresidue recognition. Our data suggest that each hot spot functions independently of the surrounding scaffold, as phage display antibody libraries using these scaffolds yielded >50 phospho- and target-specific mAbs against 70% of target peptides. Our motif-specific scaffold strategy may provide a general solution for rapid, robust development of anti-PTM mAbs for signaling, diagnostic and therapeutic applications.

-	~~==About this Structure==~~	+	Nature-inspired design of motif-specific antibody scaffolds.,Koerber JT, Thomsen ND, Hannigan BT, Degrado WF, Wells JA Nat Biotechnol. 2013 Aug 18. doi: 10.1038/nbt.2672. PMID:23955275<ref>PMID:23955275</ref>
-	~~[[4jfz]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4JFZ OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023955275</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
-	[[Category: Degrado, W F.]]	+	[[Category: Degrado, W F]]
-	[[Category: Hannigan, B T.]]	+	[[Category: Hannigan, B T]]
-	[[Category: Koerber, J T.]]	+	[[Category: Koerber, J T]]
-	[[Category: Thomsen, N D.]]	+	[[Category: Thomsen, N D]]
-	[[Category: Wells, J A.]]	+	[[Category: Wells, J A]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Immunoglobulin domain]]		[[Category: Immunoglobulin domain]]

OCA at 07:03, 26 March 2014

OCA — Wed, 26 Mar 2014 07:03:24 GMT

←Older revision		Revision as of 07:03, 26 March 2014
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	==About this Structure==		==About this Structure==
-	[[4jfz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JFZ OCA].	+	[[4jfz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JFZ OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:023955275</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:023955275</ref><references group="xtra"/><references/>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Degrado, W F.]]		[[Category: Degrado, W F.]]
	[[Category: Hannigan, B T.]]		[[Category: Hannigan, B T.]]

OCA at 07:33, 28 August 2013

OCA — Wed, 28 Aug 2013 07:33:44 GMT

←Older revision		Revision as of 07:33, 28 August 2013
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4jfz\| PDB=4jfz \| SCENE= }}
		+	===Structure of phosphoserine (pSAb) scaffold bound to pSer peptide===
		+	{{ABSTRACT_PUBMED_23955275}}

-	~~The entry~~ 4jfz is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4jfz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JFZ OCA].

-	~~Authors~~: ~~Koerber~~, J.~~T., Thomsen, N.D.,~~ Hannigan, B.T., ~~Degrado, W.F~~., Wells, J.A.	+	==Reference==
-		+	<ref group="xtra">PMID:023955275</ref><references group="xtra"/><references/>
-	~~Description~~: ~~protein complex2~~	+	[[Category: Escherichia coli]]
		+	[[Category: Degrado, W F.]]
		+	[[Category: Hannigan, B T.]]
		+	[[Category: Koerber, J T.]]
		+	[[Category: Thomsen, N D.]]
		+	[[Category: Wells, J A.]]
		+	[[Category: Immune system]]
		+	[[Category: Immunoglobulin domain]]

OCA at 16:19, 20 March 2013

OCA — Wed, 20 Mar 2013 16:19:00 GMT

←Older revision		Revision as of 16:19, 20 March 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4jfz is ON HOLD	+	The entry 4jfz is ON HOLD until Paper Publication

	Authors: Koerber, J.T., Thomsen, N.D., Hannigan, B.T., Degrado, W.F., Wells, J.A.		Authors: Koerber, J.T., Thomsen, N.D., Hannigan, B.T., Degrado, W.F., Wells, J.A.

	Description: protein complex2		Description: protein complex2

OCA: Protected "4jfz" [edit=sysop:move=sysop]

OCA — Wed, 06 Mar 2013 13:55:28 GMT

Protected "4jfz" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:55, 6 March 2013

OCA: New page: '''Unreleased structure''' The entry 4jfz is ON HOLD Authors: Koerber, J.T., Thomsen, N.D., Hannigan, B.T., Degrado, W.F., Wells, J.A. Description: protein complex2

OCA — Wed, 06 Mar 2013 13:55:27 GMT

New page: '''Unreleased structure''' The entry 4jfz is ON HOLD Authors: Koerber, J.T., Thomsen, N.D., Hannigan, B.T., Degrado, W.F., Wells, J.A. Description: protein complex2

New page

'''Unreleased structure'''

The entry 4jfz is ON HOLD

Authors: Koerber, J.T., Thomsen, N.D., Hannigan, B.T., Degrado, W.F., Wells, J.A.

Description: protein complex2