4jjz - Revision history

OCA at 12:04, 1 March 2024

OCA — Fri, 01 Mar 2024 12:04:19 GMT

←Older revision		Revision as of 12:04, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4jjz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorella_thermoacetica_ATCC_39073 Moorella thermoacetica ATCC 39073]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rbo 3rbo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JJZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4jjz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorella_thermoacetica_ATCC_39073 Moorella thermoacetica ATCC 39073]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rbo 3rbo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JJZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TOE:2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL'>TOE</scene>, <scene name='pdbligand=XPO:FORMYL+PHOSPHATE'>XPO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TOE:2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL'>TOE</scene>, <scene name='pdbligand=XPO:FORMYL+PHOSPHATE'>XPO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jjz OCA], [https://pdbe.org/4jjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jjz RCSB], [https://www.ebi.ac.uk/pdbsum/4jjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jjz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jjz OCA], [https://pdbe.org/4jjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jjz RCSB], [https://www.ebi.ac.uk/pdbsum/4jjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jjz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/FTHS_MOOTA FTHS_MOOTA]		[https://www.uniprot.org/uniprot/FTHS_MOOTA FTHS_MOOTA]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	N(10) -formyltetrahydrofolate synthetase (FTHFS) is a folate enzyme that catalyzes the formylation of tetrahydrofolate (THF) in an ATP dependent manner. Structures of FTHFS from the thermophilic homoacetogen, Moorella thermoacetica, complexed with 1) a catalytic intermediate - formylphosphate (XPO) and product - ADP; 2) with an inhibitory substrate analog - folate; 3) with XPO and an inhibitory THF analog, ZD9331, were used to analyze the enzyme mechanism. Nucleophilic attack of the formate ion on the gamma phosphate of ATP leads to the formation of XPO and the first product ADP. A channel that leads to the putative formate binding pocket allows for the binding of ATP and formate in random order. Formate binding is due to interactions with the gamma-phosphate moiety of ATP and additionally to two hydrogen bonds from the backbone nitrogen of Ala276 and the side chain of Arg97. Upon ADP dissociation, XPO reorients and moves to the position previously occupied by the beta-phosphate of ATP. Conformational changes that occur due to the XPO presence apparently allow for the recruitment of the third substrate, THF, with its pterin moiety positioned between Phe384 and Trp412. This position overlaps with that of the bound nucleoside, which is consistent with a catalytic mechanism hypothesis that FTHFS works via a sequential ping-pong mechanism. More specifically, a random bi uni uni bi ping-pong ter ter mechanism is proposed. Additionally, the native structure originally reported at a 2.5 A resolution was redetermined at a 2.2 A resolution.
-
-	Mechanism of N(10) -formyltetrahydrofolate synthetase derived from complexes with intermediates and inhibitors.,Celeste LR, Chai G, Bielak M, Minor W, Lovelace LL, Lebioda L Protein Sci. 2011 Nov 22. doi: 10.1002/pro.2005. PMID:22109967<ref>PMID:22109967</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4jjz" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:17, 24 November 2022

OCA — Thu, 24 Nov 2022 11:17:27 GMT

←Older revision		Revision as of 11:17, 24 November 2022
Line 3:		Line 3:
	<StructureSection load='4jjz' size='340' side='right'caption='[[4jjz]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4jjz' size='340' side='right'caption='[[4jjz]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4jjz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Moota Moota~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rbo 3rbo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=4JJZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4jjz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorella_thermoacetica_ATCC_39073 Moorella thermoacetica ATCC 39073]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rbo 3rbo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JJZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TOE:2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL'>TOE</scene>, <scene name='pdbligand=XPO:FORMYL+PHOSPHATE'>XPO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TOE:2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL'>TOE</scene>, <scene name='pdbligand=XPO:FORMYL+PHOSPHATE'>XPO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzx\|3pzx]], [[4jim\|4jim]], [[3rbo\|3rbo]], [[3sin\|3sin]], [[3qb6\|3qb6]], [[4jjk\|4jjk]], [[4jki\|4jki]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jjz OCA], [https://pdbe.org/4jjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jjz RCSB], [https://www.ebi.ac.uk/pdbsum/4jjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jjz ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fhs, moorella, Moth_0109 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264732 MOOTA])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=4jjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jjz OCA], [~~http~~://pdbe.org/4jjz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4jjz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4jjz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4jjz ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FTHS_MOOTA FTHS_MOOTA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Formate--tetrahydrofolate ligase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Moota~~]]	+	[[Category: Moorella thermoacetica ATCC 39073]]
-	[[Category: Celeste~~, L R~~]]	+	[[Category: Celeste LR]]
-	[[Category: Lebioda, L]]	+	[[Category: Lebioda L]]
-	[[Category: Lovelace~~, L L]]~~	+	[[Category: Lovelace LL]]
-	~~[[Category: Ligase~~]]	+

OCA at 09:43, 9 September 2020

OCA — Wed, 09 Sep 2020 09:43:42 GMT

←Older revision		Revision as of 09:43, 9 September 2020
Line 1:		Line 1:

-	==Crystal ~~Strucutre~~ of N10-Formyltetrahydrofolate Synthetase with ADP and Formylphosphate==	+	==Crystal Structure of N10-Formyltetrahydrofolate Synthetase with ADP and Formylphosphate==
-	<StructureSection load='4jjz' size='340' side='right' caption='[[4jjz]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4jjz' size='340' side='right'caption='[[4jjz]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4jjz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Moota Moota]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rbo 3rbo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4JJZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4jjz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Moota Moota]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rbo 3rbo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4JJZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TOE:2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL'>TOE</scene>, <scene name='pdbligand=XPO:FORMYL+PHOSPHATE'>XPO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TOE:2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL'>TOE</scene>, <scene name='pdbligand=XPO:FORMYL+PHOSPHATE'>XPO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzx\|3pzx]], [[4jim\|4jim]], [[3rbo\|3rbo]], [[3sin\|3sin]], [[3qb6\|3qb6]], [[4jjk\|4jjk]], [[4jki\|4jki]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzx\|3pzx]], [[4jim\|4jim]], [[3rbo\|3rbo]], [[3sin\|3sin]], [[3qb6\|3qb6]], [[4jjk\|4jjk]], [[4jki\|4jki]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fhs, moorella, Moth_0109 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264732 MOOTA])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fhs, moorella, Moth_0109 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264732 MOOTA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4jjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jjz OCA], [http://pdbe.org/4jjz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jjz RCSB], [http://www.ebi.ac.uk/pdbsum/4jjz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jjz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4jjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jjz OCA], [http://pdbe.org/4jjz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jjz RCSB], [http://www.ebi.ac.uk/pdbsum/4jjz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jjz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Formate--tetrahydrofolate ligase]]		[[Category: Formate--tetrahydrofolate ligase]]
		+	[[Category: Large Structures]]
	[[Category: Moota]]		[[Category: Moota]]
	[[Category: Celeste, L R]]		[[Category: Celeste, L R]]

OCA: Protected "4jjz" [edit=sysop:move=sysop]

OCA — Wed, 15 Nov 2017 09:11:22 GMT

Protected "4jjz" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:11, 15 November 2017

OCA: New page: ==Crystal Strucutre of N10-Formyltetrahydrofolate Synthetase with ADP and Formylphosphate== == Structural highlights ==
<table><tr><td colspan='2'>[[4jjz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Moota Moota]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rbo 3rbo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JJZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JJZ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TOE:2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL'>TOE</scene>, <scene name='pdbligand=XPO:FORMYL+PHOSPHATE'>XPO</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pzx|3pzx]], [[4jim|4jim]], [[3rbo|3rbo]], [[3sin|3sin]], [[3qb6|3qb6]], [[4jjk|4jjk]], [[4jki|4jki]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fhs, moorella, Moth_0109 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264732 MOOTA])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jjz OCA], [http://pdbe.org/4jjz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jjz RCSB], [http://www.ebi.ac.uk/pdbsum/4jjz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jjz ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
N(10) -formyltetrahydrofolate synthetase (FTHFS) is a folate enzyme that catalyzes the formylation of tetrahydrofolate (THF) in an ATP dependent manner. Structures of FTHFS from the thermophilic homoacetogen, Moorella thermoacetica, complexed with 1) a catalytic intermediate - formylphosphate (XPO) and product - ADP; 2) with an inhibitory substrate analog - folate; 3) with XPO and an inhibitory THF analog, ZD9331, were used to analyze the enzyme mechanism. Nucleophilic attack of the formate ion on the gamma phosphate of ATP leads to the formation of XPO and the first product ADP. A channel that leads to the putative formate binding pocket allows for the binding of ATP and formate in random order. Formate binding is due to interactions with the gamma-phosphate moiety of ATP and additionally to two hydrogen bonds from the backbone nitrogen of Ala276 and the side chain of Arg97. Upon ADP dissociation, XPO reorients and moves to the position previously occupied by the beta-phosphate of ATP. Conformational changes that occur due to the XPO presence apparently allow for the recruitment of the third substrate, THF, with its pterin moiety positioned between Phe384 and Trp412. This position overlaps with that of the bound nucleoside, which is consistent with a catalytic mechanism hypothesis that FTHFS works via a sequential ping-pong mechanism. More specifically, a random bi uni uni bi ping-pong ter ter mechanism is proposed. Additionally, the native structure originally reported at a 2.5 A resolution was redetermined at a 2.2 A resolution.

Mechanism of N(10) -formyltetrahydrofolate synthetase derived from complexes with intermediates and inhibitors.,Celeste LR, Chai G, Bielak M, Minor W, Lovelace LL, Lebioda L Protein Sci. 2011 Nov 22. doi: 10.1002/pro.2005. PMID:22109967<ref>PMID:22109967</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4jjz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Formate--tetrahydrofolate ligase]]
[[Category: Moota]]
[[Category: Celeste, L R]]
[[Category: Lebioda, L]]
[[Category: Lovelace, L L]]
[[Category: Ligase]]