4jls - Revision history

OCA at 12:04, 1 March 2024

OCA — Fri, 01 Mar 2024 12:04:51 GMT

←Older revision		Revision as of 12:04, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4jls]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._MDS42 Escherichia coli str. K-12 substr. MDS42]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JLS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4jls]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._MDS42 Escherichia coli str. K-12 substr. MDS42]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JLS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3ZE:{2-[(3S,4R)-3-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)-4-HYDROXYPYRROLIDIN-1-YL]-2-OXOETHYL}PHOSPHONIC+ACID'>3ZE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3ZE:{2-[(3S,4R)-3-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)-4-HYDROXYPYRROLIDIN-1-YL]-2-OXOETHYL}PHOSPHONIC+ACID'>3ZE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jls OCA], [https://pdbe.org/4jls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jls RCSB], [https://www.ebi.ac.uk/pdbsum/4jls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jls ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jls OCA], [https://pdbe.org/4jls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jls RCSB], [https://www.ebi.ac.uk/pdbsum/4jls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jls ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/XGPT_ECOLI XGPT_ECOLI] Acts on guanine, xanthine and to a lesser extent hypoxanthine.<ref>PMID:9100006</ref>		[https://www.uniprot.org/uniprot/XGPT_ECOLI XGPT_ECOLI] Acts on guanine, xanthine and to a lesser extent hypoxanthine.<ref>PMID:9100006</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Escherichia coli (Ec) cells possess two purine salvage enzymes: xanthine-guanine phosphoribosyltransferase (XGPRT) and hypoxanthine phosphoribosyltransferase (HPRT). EcXGPRT shares a common structural feature with other members of this family, a flexible loop that closes over the active site during catalysis. The replacement of six of these amino acids by alanine has no effect on the Km for the two substrates. However, the Ki for the nucleoside monophosphate increases by 27-fold, and the kcat is reduced by approximately 200-fold. Nucleoside phosphonates (NP) are good inhibitors of EcXGPRT and EcHPRT, with Ki values as low as 10 nM. In the absence of the flexible loop, these values increase by 5- to 30-fold, indicating the importance of the loop for high-affinity inhibition. Crystal structures of two NPs in complex with EcXGPRT explain the tight binding. Prodrugs of NPs with low Ki values for EcXGPRT or EcHPRT exhibit IC50 values between 5 and 23 muM against Mycobacterium tuberculosis in cell-based assays, suggesting that these compounds are therapeutic leads against pathogenic bacteria.
-
-	Inhibition of the Escherichia coli 6-oxopurine phosphoribosyltransferases by nucleoside phosphonates: potential for new antibacterial agents.,Keough DT, Hockova D, Rejman D, Spacek P, Vrbkova S, Krecmerova M, Eng WS, Jans H, West NP, Naesens LM, de Jersey J, Guddat LW J Med Chem. 2013 Sep 12;56(17):6967-84. doi: 10.1021/jm400779n. Epub 2013 Aug 27. PMID:23927482<ref>PMID:23927482</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4jls" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 11:21, 24 November 2022

OCA — Thu, 24 Nov 2022 11:21:03 GMT

←Older revision		Revision as of 11:21, 24 November 2022
Line 3:		Line 3:
	<StructureSection load='4jls' size='340' side='right'caption='[[4jls]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4jls' size='340' side='right'caption='[[4jls]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4jls]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Escherichia_coli_mds42~~ Escherichia coli ~~mds42~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JLS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4JLS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4jls]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._MDS42 Escherichia coli str. K-12 substr. MDS42]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JLS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3ZE:{2-[(3S,4R)-3-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)-4-HYDROXYPYRROLIDIN-1-YL]-2-OXOETHYL}PHOSPHONIC+ACID'>3ZE</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3ZE:{2-[(3S,4R)-3-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)-4-HYDROXYPYRROLIDIN-1-YL]-2-OXOETHYL}PHOSPHONIC+ACID'>3ZE</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4jit\|4jit]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jls OCA], [https://pdbe.org/4jls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jls RCSB], [https://www.ebi.ac.uk/pdbsum/4jls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jls ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gpt, ECMDS42_0227 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1110693 Escherichia coli MDS42])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Xanthine_phosphoribosyltransferase Xanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.22 2.4.2.22] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4jls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jls OCA], [~~http~~://pdbe.org/4jls PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4jls RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4jls PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4jls ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~H0Q6L9_ECOLI H0Q6L9_ECOLI]~~] Acts on guanine, xanthine and to a lesser extent hypoxanthine.~~[HAMAP-Rule~~:~~MF_01903][SAAS:SAAS00010659]~~	+	[https://www.uniprot.org/uniprot/XGPT_ECOLI XGPT_ECOLI] Acts on guanine, xanthine and to a lesser extent hypoxanthine.<ref>PMID:9100006</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 20:

	==See Also==		==See Also==
-	*[[Phosphoribosyltransferase\|Phosphoribosyltransferase]]	+	*[[Phosphoribosyltransferase 3D structures\|Phosphoribosyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Escherichia coli ~~mds42~~]]	+	[[Category: Escherichia coli str. K-12 substr. MDS42]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Xanthine phosphoribosyltransferase]]~~	+	[[Category: Eng WS]]
-	[[Category: Eng~~, W S~~]]	+	[[Category: Guddat LW]]
-	[[Category: Guddat~~, L W~~]]	+	[[Category: Hockova D]]
-	[[Category: Hockova, D]]	+	[[Category: Jans H]]
-	[[Category: Jans, H]]	+	[[Category: Keough DT]]
-	~~[[Category: Jersey, J de~~]]	+	[[Category: Krecmerova M]]
-	[[Category: Keough~~, D T~~]]	+	[[Category: Naesens LMJ]]
-	[[Category: Krecmerova, M]]	+	[[Category: Rejman D]]
-	[[Category: Naesens~~, L M.J~~]]	+	[[Category: Spacek P]]
-	[[Category: Rejman, D]]	+	[[Category: Vrbkova S]]
-	[[Category: Spacek, P]]	+	[[Category: West NP]]
-	[[Category: Vrbkova, S]]	+	[[Category: De Jersey J]]
-	[[Category: West~~, N P~~]]	+
-	[[Category: ~~Antibacterial]]~~	+
-	~~[[Category: Nucleoside phosphonate]]~~	+
-	~~[[Category: Purine salvage]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Xanthine-guanine phosphoribosyltransferase~~]]	+

OCA at 09:25, 3 April 2019

OCA — Wed, 03 Apr 2019 09:25:34 GMT

←Older revision		Revision as of 09:25, 3 April 2019
Line 1:		Line 1:

	==Crystal Structure of E. coli XGPRT in complex with (3R,4S)-4-(Guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylacetylphosphonic acid==		==Crystal Structure of E. coli XGPRT in complex with (3R,4S)-4-(Guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylacetylphosphonic acid==
-	<StructureSection load='4jls' size='340' side='right' caption='[[4jls]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='4jls' size='340' side='right'caption='[[4jls]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4jls]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JLS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JLS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4jls]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_mds42 Escherichia coli mds42]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JLS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JLS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3ZE:{2-[(3S,4R)-3-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)-4-HYDROXYPYRROLIDIN-1-YL]-2-OXOETHYL}PHOSPHONIC+ACID'>3ZE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3ZE:{2-[(3S,4R)-3-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)-4-HYDROXYPYRROLIDIN-1-YL]-2-OXOETHYL}PHOSPHONIC+ACID'>3ZE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4jit\|4jit]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4jit\|4jit]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gpt, ECMDS42_0227 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1110693 Escherichia coli MDS42])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Xanthine_phosphoribosyltransferase Xanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.22 2.4.2.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Xanthine_phosphoribosyltransferase Xanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.22 2.4.2.22] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jls OCA], [http://pdbe.org/4jls PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jls RCSB], [http://www.ebi.ac.uk/pdbsum/4jls PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jls ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jls OCA], [http://pdbe.org/4jls PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jls RCSB], [http://www.ebi.ac.uk/pdbsum/4jls PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jls ProSAT]</span></td></tr>
Line 27:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli mds42]]
		+	[[Category: Large Structures]]
	[[Category: Xanthine phosphoribosyltransferase]]		[[Category: Xanthine phosphoribosyltransferase]]
	[[Category: Eng, W S]]		[[Category: Eng, W S]]

OCA at 19:43, 4 August 2016

OCA — Thu, 04 Aug 2016 19:43:54 GMT

←Older revision		Revision as of 19:43, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of E. coli XGPRT in complex with (3R,4S)-4-(Guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylacetylphosphonic acid==		==Crystal Structure of E. coli XGPRT in complex with (3R,4S)-4-(Guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylacetylphosphonic acid==
	<StructureSection load='4jls' size='340' side='right' caption='[[4jls]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4jls' size='340' side='right' caption='[[4jls]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4jit\|4jit]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4jit\|4jit]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Xanthine_phosphoribosyltransferase Xanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.22 2.4.2.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Xanthine_phosphoribosyltransferase Xanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.22 2.4.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jls OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jls RCSB], [http://www.ebi.ac.uk/pdbsum/4jls PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jls OCA], [http://pdbe.org/4jls PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jls RCSB], [http://www.ebi.ac.uk/pdbsum/4jls PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jls ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4jls" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphoribosyltransferase\|Phosphoribosyltransferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:58, 24 December 2014

OCA — Wed, 24 Dec 2014 10:58:03 GMT

←Older revision		Revision as of 10:58, 24 December 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal Structure of E. coli XGPRT in complex with (3R,4S)-4-(Guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylacetylphosphonic acid==
		+	<StructureSection load='4jls' size='340' side='right' caption='[[4jls]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4jls]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JLS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JLS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3ZE:{2-[(3S,4R)-3-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)-4-HYDROXYPYRROLIDIN-1-YL]-2-OXOETHYL}PHOSPHONIC+ACID'>3ZE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4jit\|4jit]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Xanthine_phosphoribosyltransferase Xanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.22 2.4.2.22] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jls OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jls RCSB], [http://www.ebi.ac.uk/pdbsum/4jls PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/H0Q6L9_ECOLI H0Q6L9_ECOLI]] Acts on guanine, xanthine and to a lesser extent hypoxanthine.[HAMAP-Rule:MF_01903][SAAS:SAAS00010659]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Escherichia coli (Ec) cells possess two purine salvage enzymes: xanthine-guanine phosphoribosyltransferase (XGPRT) and hypoxanthine phosphoribosyltransferase (HPRT). EcXGPRT shares a common structural feature with other members of this family, a flexible loop that closes over the active site during catalysis. The replacement of six of these amino acids by alanine has no effect on the Km for the two substrates. However, the Ki for the nucleoside monophosphate increases by 27-fold, and the kcat is reduced by approximately 200-fold. Nucleoside phosphonates (NP) are good inhibitors of EcXGPRT and EcHPRT, with Ki values as low as 10 nM. In the absence of the flexible loop, these values increase by 5- to 30-fold, indicating the importance of the loop for high-affinity inhibition. Crystal structures of two NPs in complex with EcXGPRT explain the tight binding. Prodrugs of NPs with low Ki values for EcXGPRT or EcHPRT exhibit IC50 values between 5 and 23 muM against Mycobacterium tuberculosis in cell-based assays, suggesting that these compounds are therapeutic leads against pathogenic bacteria.

-	~~The entry 4jls is ON HOLD until sometime in~~ the ~~future~~	+	Inhibition of the Escherichia coli 6-oxopurine phosphoribosyltransferases by nucleoside phosphonates: potential for new antibacterial agents.,Keough DT, Hockova D, Rejman D, Spacek P, Vrbkova S, Krecmerova M, Eng WS, Jans H, West NP, Naesens LM, de Jersey J, Guddat LW J Med Chem. 2013 Sep 12;56(17):6967-84. doi: 10.1021/jm400779n. Epub 2013 Aug 27. PMID:23927482<ref>PMID:23927482</ref>

-	~~Authors: Keough~~, D.~~T., Hockova, D., Rejman, D., Spacek, P., Vrbkova,~~ S.~~, Krecmerova, M~~., Eng, W.S., Jans, H., ~~West~~, ~~N.P.~~, Naesens, L.M.J., ~~de Jersey~~, J., ~~Guddat, L.W.~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal Structure of E. coli XGPRT in complex with 3R~~,~~4S)-4-(Guanin-9-yl)-3-hydroxypyrrolidin-1-~~N-~~ylacetylphosphonic acid~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Xanthine phosphoribosyltransferase]]
		+	[[Category: Eng, W S]]
		+	[[Category: Guddat, L W]]
		+	[[Category: Hockova, D]]
		+	[[Category: Jans, H]]
		+	[[Category: Jersey, J de]]
		+	[[Category: Keough, D T]]
		+	[[Category: Krecmerova, M]]
		+	[[Category: Naesens, L M.J]]
		+	[[Category: Rejman, D]]
		+	[[Category: Spacek, P]]
		+	[[Category: Vrbkova, S]]
		+	[[Category: West, N P]]
		+	[[Category: Antibacterial]]
		+	[[Category: Nucleoside phosphonate]]
		+	[[Category: Purine salvage]]
		+	[[Category: Transferase]]
		+	[[Category: Xanthine-guanine phosphoribosyltransferase]]

OCA at 13:02, 3 July 2013

OCA — Wed, 03 Jul 2013 13:02:18 GMT

←Older revision		Revision as of 13:02, 3 July 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4jls is ON HOLD	+	The entry 4jls is ON HOLD until sometime in the future

	Authors: Keough, D.T., Hockova, D., Rejman, D., Spacek, P., Vrbkova, S., Krecmerova, M., Eng, W.S., Jans, H., West, N.P., Naesens, L.M.J., de Jersey, J., Guddat, L.W.		Authors: Keough, D.T., Hockova, D., Rejman, D., Spacek, P., Vrbkova, S., Krecmerova, M., Eng, W.S., Jans, H., West, N.P., Naesens, L.M.J., de Jersey, J., Guddat, L.W.

	Description: Crystal Structure of E. coli XGPRT in complex with 3R,4S)-4-(Guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylacetylphosphonic acid		Description: Crystal Structure of E. coli XGPRT in complex with 3R,4S)-4-(Guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylacetylphosphonic acid

OCA: Protected "4jls" [edit=sysop:move=sysop]

OCA — Wed, 20 Mar 2013 16:22:46 GMT

Protected "4jls" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:22, 20 March 2013

OCA: New page: '''Unreleased structure''' The entry 4jls is ON HOLD Authors: Keough, D.T., Hockova, D., Rejman, D., Spacek, P., Vrbkova, S., Krecmerova, M., Eng, W.S., Jans, H., West, N.P., Naesens, L...

OCA — Wed, 20 Mar 2013 16:22:45 GMT

New page: '''Unreleased structure''' The entry 4jls is ON HOLD Authors: Keough, D.T., Hockova, D., Rejman, D., Spacek, P., Vrbkova, S., Krecmerova, M., Eng, W.S., Jans, H., West, N.P., Naesens, L...

New page

'''Unreleased structure'''

The entry 4jls is ON HOLD

Authors: Keough, D.T., Hockova, D., Rejman, D., Spacek, P., Vrbkova, S., Krecmerova, M., Eng, W.S., Jans, H., West, N.P., Naesens, L.M.J., de Jersey, J., Guddat, L.W.

Description: Crystal Structure of E. coli XGPRT in complex with 3R,4S)-4-(Guanin-9-yl)-3-hydroxypyrrolidin-1-N-ylacetylphosphonic acid