4k1y - Revision history

OCA at 12:07, 1 March 2024

OCA — Fri, 01 Mar 2024 12:07:54 GMT

←Older revision		Revision as of 12:07, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k1y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K1Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k1y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K1Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1y OCA], [https://pdbe.org/4k1y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k1y RCSB], [https://www.ebi.ac.uk/pdbsum/4k1y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k1y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1y OCA], [https://pdbe.org/4k1y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k1y RCSB], [https://www.ebi.ac.uk/pdbsum/4k1y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k1y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/LECA_CANBL LECA_CANBL] D-mannose/D-glucose-binding lectin (PubMed:24865454). Has anti-inflammatory activity in animal models when applied intravenously (PubMed:24865454). Has antinociceptive activity in mice when applied intravenously (PubMed:19705102).<ref>PMID:19705102</ref> <ref>PMID:24865454</ref>		[https://www.uniprot.org/uniprot/LECA_CANBL LECA_CANBL] D-mannose/D-glucose-binding lectin (PubMed:24865454). Has anti-inflammatory activity in animal models when applied intravenously (PubMed:24865454). Has antinociceptive activity in mice when applied intravenously (PubMed:19705102).<ref>PMID:19705102</ref> <ref>PMID:24865454</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Plant lectins, especially those purified from species of the Leguminosae family, represent the best-studied group of carbohydrate-binding proteins. Lectins purified from seeds of the Diocleinae subtribe exhibit a high degree of sequence identity notwithstanding that they show very distinct biological activities. Two main factors have been related to this feature: variance in key residues influencing the carbohydrate-binding site geometry and differences in the pH-dependent oligomeric state profile. In this work, we have isolated a lectin from Canavalia boliviana (Cbol) and solved its x-ray crystal structure in the unbound form and in complex with the carbohydrates Man(alpha1-3)Man(alpha1-O)Me, Man(alpha1-4)Man(alpha1-O)Me and 5-bromo-4-chloro-3-indolyl-alpha-D-mannose. We evaluated its oligomerization profile at different pH values using Small Angle X-ray Scattering and compared it to that of Concanavalin A. Based on predicted pKa-shifts of amino acids in the subunit interfaces we devised a model for the dimer-tetramer equilibrium phenomena of these proteins. Additionally, we demonstrated Cbol anti-inflammatory properties and further characterized them using in vivo and in vitro models.
-
-	Structural Studies of an Anti-Inflammatory Lectin from Canavalia boliviana Seeds in Complex with Dimannosides.,Bezerra GA, Viertlmayr R, Moura TR, Delatorre P, Rocha BA, do Nascimento KS, Figueiredo JG, Bezerra IG, Teixeira CS, Simoes RC, Nagano CS, de Alencar NM, Gruber K, Cavada BS PLoS One. 2014 May 27;9(5):e97015. doi: 10.1371/journal.pone.0097015. eCollection, 2014. PMID:24865454<ref>PMID:24865454</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4k1y" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:36, 30 November 2022

OCA — Wed, 30 Nov 2022 11:36:50 GMT

←Older revision		Revision as of 11:36, 30 November 2022
Line 3:		Line 3:
	<StructureSection load='4k1y' size='340' side='right'caption='[[4k1y]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4k1y' size='340' side='right'caption='[[4k1y]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4k1y]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4K1Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4k1y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K1Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=~~MDM~~:~~METHYL-O3-(~~ALPHA-D-MANNOSE)-~~ALPHA-D~~-MANNOSE'>~~MDM~~</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1z\|4k1z]], [[4k20\|4k20]], [[4k21\|4k21]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1y OCA], [https://pdbe.org/4k1y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k1y RCSB], [https://www.ebi.ac.uk/pdbsum/4k1y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k1y ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4k1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1y OCA], [~~http~~://pdbe.org/4k1y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4k1y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4k1y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4k1y ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LECA_CANBL LECA_CANBL] D-mannose/D-glucose-binding lectin (PubMed:24865454). Has anti-inflammatory activity in animal models when applied intravenously (PubMed:24865454). Has antinociceptive activity in mice when applied intravenously (PubMed:19705102).<ref>PMID:19705102</ref> <ref>PMID:24865454</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 24:
	[[Category: Canavalia boliviana]]		[[Category: Canavalia boliviana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bezerra~~, G A~~]]	+	[[Category: Bezerra GA]]
-	[[Category: Cavada~~, B S~~]]	+	[[Category: Cavada BS]]
-	[[Category: Delatorre, P]]	+	[[Category: Delatorre P]]
-	[[Category: Gruber, K]]	+	[[Category: Gruber K]]
-	[[Category: Moura~~, T R~~]]	+	[[Category: Moura TR]]
-	[[Category: Rocha~~, B A.M~~]]	+	[[Category: Rocha BAM]]
-	[[Category: Viertlmayr, R]]	+	[[Category: Viertlmayr R]]
-	~~[[Category: Binding site]]~~	+
-	~~[[Category: Dimannoside]]~~	+
-	~~[[Category: Diocleinae lectin]]~~	+
-	~~[[Category: Mannose binding protein]]~~	+
-	~~[[Category: Oligomerization~~]]	+

OCA at 09:41, 3 April 2019

OCA — Wed, 03 Apr 2019 09:41:18 GMT

←Older revision		Revision as of 09:41, 3 April 2019
Line 1:		Line 1:

	==Crystal structure of Canavalia boliviana lectin in complex with Man1-3Man-OMe==		==Crystal structure of Canavalia boliviana lectin in complex with Man1-3Man-OMe==
-	<StructureSection load='4k1y' size='340' side='right' caption='[[4k1y]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4k1y' size='340' side='right'caption='[[4k1y]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k1y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K1Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k1y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K1Y FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Canavalia boliviana]]		[[Category: Canavalia boliviana]]
		+	[[Category: Large Structures]]
	[[Category: Bezerra, G A]]		[[Category: Bezerra, G A]]
	[[Category: Cavada, B S]]		[[Category: Cavada, B S]]

OCA at 19:26, 11 August 2016

OCA — Thu, 11 Aug 2016 19:26:09 GMT

←Older revision		Revision as of 19:26, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Canavalia boliviana lectin in complex with Man1-3Man-OMe==		==Crystal structure of Canavalia boliviana lectin in complex with Man1-3Man-OMe==
	<StructureSection load='4k1y' size='340' side='right' caption='[[4k1y]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4k1y' size='340' side='right' caption='[[4k1y]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MDM:METHYL-O3-(ALPHA-D-MANNOSE)-ALPHA-D-MANNOSE'>MDM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MDM:METHYL-O3-(ALPHA-D-MANNOSE)-ALPHA-D-MANNOSE'>MDM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1z\|4k1z]], [[4k20\|4k20]], [[4k21\|4k21]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1z\|4k1z]], [[4k20\|4k20]], [[4k21\|4k21]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k1y RCSB], [http://www.ebi.ac.uk/pdbsum/4k1y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1y OCA], [http://pdbe.org/4k1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k1y RCSB], [http://www.ebi.ac.uk/pdbsum/4k1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k1y ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4k1y" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:27, 4 January 2015

OCA — Sun, 04 Jan 2015 14:27:17 GMT

←Older revision		Revision as of 14:27, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k1y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K1Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k1y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K1Y FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MDM:METHYL-O3-(ALPHA-D-MANNOSE)-ALPHA-D-MANNOSE'>MDM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MDM:METHYL-O3-(ALPHA-D-MANNOSE)-ALPHA-D-MANNOSE'>MDM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1z\|4k1z]], [[4k20\|4k20]], [[4k21\|4k21]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1z\|4k1z]], [[4k20\|4k20]], [[4k21\|4k21]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k1y RCSB], [http://www.ebi.ac.uk/pdbsum/4k1y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k1y RCSB], [http://www.ebi.ac.uk/pdbsum/4k1y PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Canavalia boliviana]]		[[Category: Canavalia boliviana]]
-	[[Category: Bezerra, G A.]]	+	[[Category: Bezerra, G A]]
-	[[Category: Cavada, B S.]]	+	[[Category: Cavada, B S]]
-	[[Category: Delatorre, P.]]	+	[[Category: Delatorre, P]]
-	[[Category: Gruber, K.]]	+	[[Category: Gruber, K]]
-	[[Category: Moura, T R.]]	+	[[Category: Moura, T R]]
-	[[Category: Rocha, B A.M.]]	+	[[Category: Rocha, B A.M]]
-	[[Category: Viertlmayr, R.]]	+	[[Category: Viertlmayr, R]]
	[[Category: Binding site]]		[[Category: Binding site]]
	[[Category: Dimannoside]]		[[Category: Dimannoside]]

OCA at 07:10, 11 June 2014

OCA — Wed, 11 Jun 2014 07:10:35 GMT

←Older revision		Revision as of 07:10, 11 June 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4k1y\| PDB=4k1y \| SCENE= }}~~	+	==Crystal structure of Canavalia boliviana lectin in complex with Man1-3Man-OMe==
-	===Crystal structure of Canavalia boliviana lectin in complex with Man1-3Man-OMe===	+	<StructureSection load='4k1y' size='340' side='right' caption='[[4k1y]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4k1y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K1Y FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MDM:METHYL-O3-(ALPHA-D-MANNOSE)-ALPHA-D-MANNOSE'>MDM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1z\|4k1z]], [[4k20\|4k20]], [[4k21\|4k21]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k1y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k1y RCSB], [http://www.ebi.ac.uk/pdbsum/4k1y PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plant lectins, especially those purified from species of the Leguminosae family, represent the best-studied group of carbohydrate-binding proteins. Lectins purified from seeds of the Diocleinae subtribe exhibit a high degree of sequence identity notwithstanding that they show very distinct biological activities. Two main factors have been related to this feature: variance in key residues influencing the carbohydrate-binding site geometry and differences in the pH-dependent oligomeric state profile. In this work, we have isolated a lectin from Canavalia boliviana (Cbol) and solved its x-ray crystal structure in the unbound form and in complex with the carbohydrates Man(alpha1-3)Man(alpha1-O)Me, Man(alpha1-4)Man(alpha1-O)Me and 5-bromo-4-chloro-3-indolyl-alpha-D-mannose. We evaluated its oligomerization profile at different pH values using Small Angle X-ray Scattering and compared it to that of Concanavalin A. Based on predicted pKa-shifts of amino acids in the subunit interfaces we devised a model for the dimer-tetramer equilibrium phenomena of these proteins. Additionally, we demonstrated Cbol anti-inflammatory properties and further characterized them using in vivo and in vitro models.

-	~~==About this Structure==~~	+	Structural Studies of an Anti-Inflammatory Lectin from Canavalia boliviana Seeds in Complex with Dimannosides.,Bezerra GA, Viertlmayr R, Moura TR, Delatorre P, Rocha BA, do Nascimento KS, Figueiredo JG, Bezerra IG, Teixeira CS, Simoes RC, Nagano CS, de Alencar NM, Gruber K, Cavada BS PLoS One. 2014 May 27;9(5):e97015. doi: 10.1371/journal.pone.0097015. eCollection, 2014. PMID:24865454<ref>PMID:24865454</ref>
-	~~[[4k1y]] is a 4 chain structure with sequence~~ from ~~[http~~:~~//en~~.~~wikipedia~~.~~org/wiki~~/~~Canavalia_boliviana Canavalia boliviana]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id~~=~~4K1Y OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Canavalia boliviana]]		[[Category: Canavalia boliviana]]
	[[Category: Bezerra, G A.]]		[[Category: Bezerra, G A.]]

OCA at 06:42, 9 April 2014

OCA — Wed, 09 Apr 2014 06:42:05 GMT

←Older revision		Revision as of 06:42, 9 April 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4k1y\| PDB=4k1y \| SCENE= }}
		+	===Crystal structure of Canavalia boliviana lectin in complex with Man1-3Man-OMe===

-	~~The entry~~ 4k1y is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4k1y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K1Y OCA].
-	~~Authors~~: Bezerra, G.A., ~~Viertlmayr~~, R., Moura, T.R.~~, Delatorre, P.,~~ Rocha, B.A.M., ~~Cavada, B.S., Gruber, K~~.	+	[[Category: Canavalia boliviana]]
-		+	[[Category: Bezerra, G A.]]
-	~~Description~~: ~~Crystal structure of Canavalia boliviana~~ lectin ~~in complex with Man1-3Man-OMe~~	+	[[Category: Cavada, B S.]]
		+	[[Category: Delatorre, P.]]
		+	[[Category: Gruber, K.]]
		+	[[Category: Moura, T R.]]
		+	[[Category: Rocha, B A.M.]]
		+	[[Category: Viertlmayr, R.]]
		+	[[Category: Binding site]]
		+	[[Category: Dimannoside]]
		+	[[Category: Diocleinae lectin]]
		+	[[Category: Mannose binding protein]]
		+	[[Category: Oligomerization]]

OCA at 11:25, 19 June 2013

OCA — Wed, 19 Jun 2013 11:25:47 GMT

←Older revision		Revision as of 11:25, 19 June 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4k1y is ON HOLD	+	The entry 4k1y is ON HOLD until Paper Publication

	Authors: Bezerra, G.A., Viertlmayr, R., Moura, T.R., Delatorre, P., Rocha, B.A.M., Cavada, B.S., Gruber, K.		Authors: Bezerra, G.A., Viertlmayr, R., Moura, T.R., Delatorre, P., Rocha, B.A.M., Cavada, B.S., Gruber, K.

	Description: Crystal structure of Canavalia boliviana lectin in complex with Man1-3Man-OMe		Description: Crystal structure of Canavalia boliviana lectin in complex with Man1-3Man-OMe

OCA: Protected "4k1y" [edit=sysop:move=sysop]

OCA — Wed, 17 Apr 2013 20:06:14 GMT

Protected "4k1y" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:06, 17 April 2013

OCA: New page: '''Unreleased structure''' The entry 4k1y is ON HOLD Authors: Bezerra, G.A., Viertlmayr, R., Moura, T.R., Delatorre, P., Rocha, B.A.M., Cavada, B.S., Gruber, K. Description: Crystal st...

OCA — Wed, 17 Apr 2013 20:06:13 GMT

New page: '''Unreleased structure''' The entry 4k1y is ON HOLD Authors: Bezerra, G.A., Viertlmayr, R., Moura, T.R., Delatorre, P., Rocha, B.A.M., Cavada, B.S., Gruber, K. Description: Crystal st...

New page

'''Unreleased structure'''

The entry 4k1y is ON HOLD

Authors: Bezerra, G.A., Viertlmayr, R., Moura, T.R., Delatorre, P., Rocha, B.A.M., Cavada, B.S., Gruber, K.

Description: Crystal structure of Canavalia boliviana lectin in complex with Man1-3Man-OMe