4k4b - Revision history

OCA at 15:53, 20 September 2023

OCA — Wed, 20 Sep 2023 15:53:50 GMT

←Older revision		Revision as of 15:53, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k4b]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K4B FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k4b]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K4B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [https://pdbe.org/4k4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [https://www.ebi.ac.uk/pdbsum/4k4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [https://pdbe.org/4k4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [https://www.ebi.ac.uk/pdbsum/4k4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4b ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:39, 30 November 2022

OCA — Wed, 30 Nov 2022 11:39:36 GMT

←Older revision		Revision as of 11:39, 30 November 2022
Line 3:		Line 3:
	<StructureSection load='4k4b' size='340' side='right'caption='[[4k4b]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4k4b' size='340' side='right'caption='[[4k4b]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4k4b]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4K4B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4k4b]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K4B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sbk\|1sbk]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4c\|4k4c]], [[4k4d\|4k4d]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [https://pdbe.org/4k4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [https://www.ebi.ac.uk/pdbsum/4k4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4b ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1686, JW1676, ydiI, YdiL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [~~http~~://pdbe.org/4k4b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4k4b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4b ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~YDII_ECOLI YDII_ECOLI~~]~~] Displays esterase~~ activity toward ~~palmitoyl~~-CoA and ~~pNP~~-~~butyrate~~.	+	[https://www.uniprot.org/uniprot/MENI_ECOLI MENI_ECOLI] Catalyzes the hydrolysis of 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA) to 1,4-dihydroxy-2-naphthoate (DHNA). Also shows significant activity toward a wide range of acyl-CoA thioesters, and minimal activity toward benzoyl-holoEntB.<ref>PMID:15808744</ref> <ref>PMID:23564174</ref> <ref>PMID:24992697</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Allen~~, K N~~]]	+	[[Category: Allen KN]]
-	[[Category: Dunaway-Mariano, D]]	+	[[Category: Dunaway-Mariano D]]
-	[[Category: Farelli~~, J D~~]]	+	[[Category: Farelli JD]]
-	[[Category: Ru, W]]	+	[[Category: Ru W]]
-	~~[[Category: Hotdog fold]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Thioesterase~~]]	+

OCA at 09:46, 3 April 2019

OCA — Wed, 03 Apr 2019 09:46:16 GMT

←Older revision		Revision as of 09:46, 3 April 2019
Line 1:		Line 1:

	==X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA==		==X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA==
-	<StructureSection load='4k4b' size='340' side='right' caption='[[4k4b]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='4k4b' size='340' side='right'caption='[[4k4b]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4k4b]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K4B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4k4b]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K4B FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sbk\|1sbk]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4c\|4k4c]], [[4k4d\|4k4d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sbk\|1sbk]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4c\|4k4c]], [[4k4d\|4k4d]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1686, JW1676, ydiI, YdiL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [http://pdbe.org/4k4b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [http://www.ebi.ac.uk/pdbsum/4k4b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [http://pdbe.org/4k4b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [http://www.ebi.ac.uk/pdbsum/4k4b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4b ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Allen, K N]]		[[Category: Allen, K N]]
	[[Category: Dunaway-Mariano, D]]		[[Category: Dunaway-Mariano, D]]

OCA at 19:59, 4 August 2016

OCA — Thu, 04 Aug 2016 19:59:39 GMT

←Older revision		Revision as of 19:59, 4 August 2016
Line 1:		Line 1:
		+
	==X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA==		==X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA==
	<StructureSection load='4k4b' size='340' side='right' caption='[[4k4b]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4k4b' size='340' side='right' caption='[[4k4b]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sbk\|1sbk]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4c\|4k4c]], [[4k4d\|4k4d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sbk\|1sbk]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4c\|4k4c]], [[4k4d\|4k4d]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [http://www.ebi.ac.uk/pdbsum/4k4b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [http://pdbe.org/4k4b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [http://www.ebi.ac.uk/pdbsum/4k4b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4k4b" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:04, 24 December 2014

OCA — Wed, 24 Dec 2014 19:04:59 GMT

←Older revision		Revision as of 19:04, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k4b]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K4B FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k4b]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K4B FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sbk\|1sbk]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4c\|4k4c]], [[4k4d\|4k4d]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sbk\|1sbk]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4c\|4k4c]], [[4k4d\|4k4d]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [http://www.ebi.ac.uk/pdbsum/4k4b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [http://www.ebi.ac.uk/pdbsum/4k4b PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/YDII_ECOLI YDII_ECOLI]] Displays esterase activity toward palmitoyl-CoA and pNP-butyrate.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Allen, K N.]]	+	[[Category: Allen, K N]]
-	[[Category: Dunaway-Mariano, D.]]	+	[[Category: Dunaway-Mariano, D]]
-	[[Category: Farelli, J D.]]	+	[[Category: Farelli, J D]]
-	[[Category: Ru, W.]]	+	[[Category: Ru, W]]
	[[Category: Hotdog fold]]		[[Category: Hotdog fold]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Thioesterase]]		[[Category: Thioesterase]]

OCA at 07:41, 30 July 2014

OCA — Wed, 30 Jul 2014 07:41:39 GMT

←Older revision		Revision as of 07:41, 30 July 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA==
		+	<StructureSection load='4k4b' size='340' side='right' caption='[[4k4b]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4k4b]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K4B FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UOQ:UNDECA-2-ONE+COENZYME+A'>UOQ</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1sbk\|1sbk]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4c\|4k4c]], [[4k4d\|4k4d]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k4b RCSB], [http://www.ebi.ac.uk/pdbsum/4k4b PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Herein, the structural determinants for substrate recognition and catalysis in two hotdog-fold thioesterase paralogs, YbdB and YdiI from Escherichia coli, are identified and analyzed to provide insight into the evolution of biological function in the hotdog-fold enzyme superfamily. The X-ray crystal structures of YbdB and YdiI, in complex with inert substrate analogs, determined in this study revealed the locations of the respective thioester substrate binding sites and the identity of the residues positioned for substrate binding and catalysis. The importance of each of these residues was assessed through amino acid replacements followed by steady-state kinetic analyses of the corresponding site-directed mutants. Transient kinetic and solvent (18)O-labeling studies were then carried out to provide insight into the role of Glu63 posited to function as the nucleophile or general base in catalysis. Finally, the structure-function-mechanism profiles of the two paralogs, along with that of a more distant homolog, were compared to identify conserved elements of substrate recognition and catalysis, which define the core traits of the hotdog-fold thioesterase family, as well as structural features that are unique to each thioesterase. Founded on the insight gained from this analysis, we conclude that the promiscuity revealed by in vitro substrate activity determinations, and posited to facilitate the evolution of new biological function, is the product of intrinsic plasticity in substrate binding as well as in the catalytic mechanism.

-	~~The entry 4k4b is ON HOLD until Sep 22 2015~~	+	Structure and Catalysis in the Escherichia coli Hotdog-fold Thioesterase Paralogs YdiI and YbdB.,Wu R, Latham JA, Chen D, Farelli J, Zhao H, Matthews K, Allen KN, Dunaway-Mariano D Biochemistry. 2014 Jul 29;53(29):4788-805. doi: 10.1021/bi500334v. Epub 2014 Jul , 18. PMID:25010423<ref>PMID:25010423</ref>

-	~~Authors: Ru~~, W.~~, Farelli, J~~.D., Dunaway-Mariano, D., ~~Allen~~, ~~K.N~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Allen, K N.]]
		+	[[Category: Dunaway-Mariano, D.]]
		+	[[Category: Farelli, J D.]]
		+	[[Category: Ru, W.]]
		+	[[Category: Hotdog fold]]
		+	[[Category: Hydrolase]]
		+	[[Category: Thioesterase]]

OCA at 07:07, 23 April 2014

OCA — Wed, 23 Apr 2014 07:07:51 GMT

←Older revision		Revision as of 07:07, 23 April 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4k4b is ON HOLD until ~~Jul 01~~ 2015	+	The entry 4k4b is ON HOLD until Sep 22 2015

	Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.		Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.

	Description: X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA		Description: X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA

OCA at 08:49, 16 April 2014

OCA — Wed, 16 Apr 2014 08:49:31 GMT

←Older revision		Revision as of 08:49, 16 April 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4k4b is ON HOLD until ~~sometime in the future~~	+	The entry 4k4b is ON HOLD until Jul 01 2015

	Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.		Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.

	Description: X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA		Description: X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA

OCA at 14:58, 10 July 2013

OCA — Wed, 10 Jul 2013 14:58:24 GMT

←Older revision		Revision as of 14:58, 10 July 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4k4b is ON HOLD until ~~Paper Publication~~	+	The entry 4k4b is ON HOLD until sometime in the future

	Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.		Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.

	Description: X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA		Description: X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA

OCA at 08:47, 30 June 2013

OCA — Sun, 30 Jun 2013 08:47:07 GMT

←Older revision		Revision as of 08:47, 30 June 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4k4b is ON HOLD	+	The entry 4k4b is ON HOLD until Paper Publication

	Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.		Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.

	Description: X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA		Description: X-ray crystal structure of E. coli YdiI complexed with undeca-2-one-CoA