4k4d - Revision history

OCA at 15:53, 20 September 2023

OCA — Wed, 20 Sep 2023 15:53:54 GMT

←Older revision		Revision as of 15:53, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k4d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K4D FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k4d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K4D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HFQ:2,4-DIHYDROXYPHENACYL+COENZYME+A'>HFQ</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.17Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HFQ:2,4-DIHYDROXYPHENACYL+COENZYME+A'>HFQ</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4d OCA], [https://pdbe.org/4k4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k4d RCSB], [https://www.ebi.ac.uk/pdbsum/4k4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4d OCA], [https://pdbe.org/4k4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k4d RCSB], [https://www.ebi.ac.uk/pdbsum/4k4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4d ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:39, 30 November 2022

OCA — Wed, 30 Nov 2022 11:39:42 GMT

←Older revision		Revision as of 11:39, 30 November 2022
Line 3:		Line 3:
	<StructureSection load='4k4d' size='340' side='right'caption='[[4k4d]], [[Resolution\|resolution]] 2.17Å' scene=''>		<StructureSection load='4k4d' size='340' side='right'caption='[[4k4d]], [[Resolution\|resolution]] 2.17Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4k4d]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4K4D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4k4d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K4D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HFQ:2,4-DIHYDROXYPHENACYL+COENZYME+A'>HFQ</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HFQ:2,4-DIHYDROXYPHENACYL+COENZYME+A'>HFQ</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vh9\|1vh9]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4b\|4k4b]], [[4k4c\|4k4c]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4d OCA], [https://pdbe.org/4k4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k4d RCSB], [https://www.ebi.ac.uk/pdbsum/4k4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4d ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0597, entH, JW0589, ybdB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4d OCA], [~~http~~://pdbe.org/4k4d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4k4d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4k4d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4d ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ENTH_ECOLI ENTH_ECOLI]] Required for optimal enterobactin synthesis. Acts as a proofreading enzyme that prevents EntB misacylation by hydrolyzing the thioester bound existing between EntB and wrongly charged molecules. Displays esterase activity toward a wide range of substrates, including acyl-CoAs and aryl-CoAs.<ref>PMID:15808744</ref> <ref>PMID:17675380</ref> <ref>PMID:19119850</ref> <ref>PMID:19193103</ref>	+	[https://www.uniprot.org/uniprot/ENTH_ECOLI ENTH_ECOLI] Required for optimal enterobactin synthesis. Acts as a proofreading enzyme that prevents EntB misacylation by hydrolyzing the thioester bound existing between EntB and wrongly charged molecules. Displays esterase activity toward a wide range of substrates, including acyl-CoAs and aryl-CoAs.<ref>PMID:15808744</ref> <ref>PMID:17675380</ref> <ref>PMID:19119850</ref> <ref>PMID:19193103</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Allen~~, K N~~]]	+	[[Category: Allen KN]]
-	[[Category: Dunaway-Mariano, D]]	+	[[Category: Dunaway-Mariano D]]
-	[[Category: Farelli~~, J D~~]]	+	[[Category: Farelli JD]]
-	[[Category: Ru, W]]	+	[[Category: Ru W]]
-	~~[[Category: Hotdog fold]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Thioeatsrase~~]]	+

OCA at 08:48, 3 April 2019

OCA — Wed, 03 Apr 2019 08:48:46 GMT

←Older revision		Revision as of 08:48, 3 April 2019
Line 1:		Line 1:

	==X-ray crystal structure of E. coli YbdB complexed with 2,4-dihydroxyphenacyl-CoA==		==X-ray crystal structure of E. coli YbdB complexed with 2,4-dihydroxyphenacyl-CoA==
-	<StructureSection load='4k4d' size='340' side='right' caption='[[4k4d]], [[Resolution\|resolution]] 2.17Å' scene=''>	+	<StructureSection load='4k4d' size='340' side='right'caption='[[4k4d]], [[Resolution\|resolution]] 2.17Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4k4d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K4D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4k4d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K4D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HFQ:2,4-DIHYDROXYPHENACYL+COENZYME+A'>HFQ</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HFQ:2,4-DIHYDROXYPHENACYL+COENZYME+A'>HFQ</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vh9\|1vh9]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4b\|4k4b]], [[4k4c\|4k4c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vh9\|1vh9]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4b\|4k4b]], [[4k4c\|4k4c]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0597, entH, JW0589, ybdB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4d OCA], [http://pdbe.org/4k4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k4d RCSB], [http://www.ebi.ac.uk/pdbsum/4k4d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4d OCA], [http://pdbe.org/4k4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k4d RCSB], [http://www.ebi.ac.uk/pdbsum/4k4d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4d ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Allen, K N]]		[[Category: Allen, K N]]
	[[Category: Dunaway-Mariano, D]]		[[Category: Dunaway-Mariano, D]]

OCA at 12:19, 4 August 2016

OCA — Thu, 04 Aug 2016 12:19:49 GMT

←Older revision		Revision as of 12:19, 4 August 2016
Line 1:		Line 1:
		+
	==X-ray crystal structure of E. coli YbdB complexed with 2,4-dihydroxyphenacyl-CoA==		==X-ray crystal structure of E. coli YbdB complexed with 2,4-dihydroxyphenacyl-CoA==
	<StructureSection load='4k4d' size='340' side='right' caption='[[4k4d]], [[Resolution\|resolution]] 2.17Å' scene=''>		<StructureSection load='4k4d' size='340' side='right' caption='[[4k4d]], [[Resolution\|resolution]] 2.17Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HFQ:2,4-DIHYDROXYPHENACYL+COENZYME+A'>HFQ</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HFQ:2,4-DIHYDROXYPHENACYL+COENZYME+A'>HFQ</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vh9\|1vh9]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4b\|4k4b]], [[4k4c\|4k4c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vh9\|1vh9]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4b\|4k4b]], [[4k4c\|4k4c]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k4d RCSB], [http://www.ebi.ac.uk/pdbsum/4k4d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4d OCA], [http://pdbe.org/4k4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k4d RCSB], [http://www.ebi.ac.uk/pdbsum/4k4d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k4d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4k4d" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:26, 24 December 2014

OCA — Wed, 24 Dec 2014 14:26:07 GMT

←Older revision		Revision as of 14:26, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k4d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K4D FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k4d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K4D FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HFQ:2,4-DIHYDROXYPHENACYL+COENZYME+A'>HFQ</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HFQ:2,4-DIHYDROXYPHENACYL+COENZYME+A'>HFQ</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vh9\|1vh9]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4b\|4k4b]], [[4k4c\|4k4c]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vh9\|1vh9]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4b\|4k4b]], [[4k4c\|4k4c]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k4d RCSB], [http://www.ebi.ac.uk/pdbsum/4k4d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k4d RCSB], [http://www.ebi.ac.uk/pdbsum/4k4d PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ENTH_ECOLI ENTH_ECOLI]] Required for optimal enterobactin synthesis. Acts as a proofreading enzyme that prevents EntB misacylation by hydrolyzing the thioester bound existing between EntB and wrongly charged molecules. Displays esterase activity toward a wide range of substrates, including acyl-CoAs and aryl-CoAs.<ref>PMID:15808744</ref> <ref>PMID:17675380</ref> <ref>PMID:19119850</ref> <ref>PMID:19193103</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Allen, K N.]]	+	[[Category: Allen, K N]]
-	[[Category: Dunaway-Mariano, D.]]	+	[[Category: Dunaway-Mariano, D]]
-	[[Category: Farelli, J D.]]	+	[[Category: Farelli, J D]]
-	[[Category: Ru, W.]]	+	[[Category: Ru, W]]
	[[Category: Hotdog fold]]		[[Category: Hotdog fold]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Thioeatsrase]]		[[Category: Thioeatsrase]]

OCA at 07:43, 30 July 2014

OCA — Wed, 30 Jul 2014 07:43:07 GMT

←Older revision		Revision as of 07:43, 30 July 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==X-ray crystal structure of E. coli YbdB complexed with 2,4-dihydroxyphenacyl-CoA==
		+	<StructureSection load='4k4d' size='340' side='right' caption='[[4k4d]], [[Resolution\|resolution]] 2.17Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4k4d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K4D FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HFQ:2,4-DIHYDROXYPHENACYL+COENZYME+A'>HFQ</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vh9\|1vh9]], [[4k49\|4k49]], [[4k4a\|4k4a]], [[4k4b\|4k4b]], [[4k4c\|4k4c]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k4d RCSB], [http://www.ebi.ac.uk/pdbsum/4k4d PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Herein, the structural determinants for substrate recognition and catalysis in two hotdog-fold thioesterase paralogs, YbdB and YdiI from Escherichia coli, are identified and analyzed to provide insight into the evolution of biological function in the hotdog-fold enzyme superfamily. The X-ray crystal structures of YbdB and YdiI, in complex with inert substrate analogs, determined in this study revealed the locations of the respective thioester substrate binding sites and the identity of the residues positioned for substrate binding and catalysis. The importance of each of these residues was assessed through amino acid replacements followed by steady-state kinetic analyses of the corresponding site-directed mutants. Transient kinetic and solvent (18)O-labeling studies were then carried out to provide insight into the role of Glu63 posited to function as the nucleophile or general base in catalysis. Finally, the structure-function-mechanism profiles of the two paralogs, along with that of a more distant homolog, were compared to identify conserved elements of substrate recognition and catalysis, which define the core traits of the hotdog-fold thioesterase family, as well as structural features that are unique to each thioesterase. Founded on the insight gained from this analysis, we conclude that the promiscuity revealed by in vitro substrate activity determinations, and posited to facilitate the evolution of new biological function, is the product of intrinsic plasticity in substrate binding as well as in the catalytic mechanism.

-	~~The entry 4k4d is ON HOLD until Sep 22 2015~~	+	Structure and Catalysis in the Escherichia coli Hotdog-fold Thioesterase Paralogs YdiI and YbdB.,Wu R, Latham JA, Chen D, Farelli J, Zhao H, Matthews K, Allen KN, Dunaway-Mariano D Biochemistry. 2014 Jul 29;53(29):4788-805. doi: 10.1021/bi500334v. Epub 2014 Jul , 18. PMID:25010423<ref>PMID:25010423</ref>

-	~~Authors: Ru~~, W.~~, Farelli, J~~.D., Dunaway-Mariano, D., ~~Allen~~, ~~K.N~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~X-ray crystal structure of E. coli YbdB complexed with 2,4-dihydroxyphenacyl-CoA~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Allen, K N.]]
		+	[[Category: Dunaway-Mariano, D.]]
		+	[[Category: Farelli, J D.]]
		+	[[Category: Ru, W.]]
		+	[[Category: Hotdog fold]]
		+	[[Category: Hydrolase]]
		+	[[Category: Thioeatsrase]]

OCA at 07:07, 23 April 2014

OCA — Wed, 23 Apr 2014 07:07:55 GMT

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-	The entry 4k4d is ON HOLD until ~~Paper Publication~~	+	The entry 4k4d is ON HOLD until Sep 22 2015

	Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.		Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.

	Description: X-ray crystal structure of E. coli YbdB complexed with 2,4-dihydroxyphenacyl-CoA		Description: X-ray crystal structure of E. coli YbdB complexed with 2,4-dihydroxyphenacyl-CoA

OCA at 08:47, 30 June 2013

OCA — Sun, 30 Jun 2013 08:47:13 GMT

←Older revision		Revision as of 08:47, 30 June 2013
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-	The entry 4k4d is ON HOLD	+	The entry 4k4d is ON HOLD until Paper Publication

	Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.		Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.

	Description: X-ray crystal structure of E. coli YbdB complexed with 2,4-dihydroxyphenacyl-CoA		Description: X-ray crystal structure of E. coli YbdB complexed with 2,4-dihydroxyphenacyl-CoA

OCA: Protected "4k4d" [edit=sysop:move=sysop]

OCA — Wed, 24 Apr 2013 10:58:43 GMT

Protected "4k4d" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:58, 24 April 2013

OCA: New page: '''Unreleased structure''' The entry 4k4d is ON HOLD Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N. Description: X-ray crystal structure of E. coli YbdB complexed wit...

OCA — Wed, 24 Apr 2013 10:58:42 GMT

New page: '''Unreleased structure''' The entry 4k4d is ON HOLD Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N. Description: X-ray crystal structure of E. coli YbdB complexed wit...

New page

'''Unreleased structure'''

The entry 4k4d is ON HOLD

Authors: Ru, W., Farelli, J.D., Dunaway-Mariano, D., Allen, K.N.

Description: X-ray crystal structure of E. coli YbdB complexed with 2,4-dihydroxyphenacyl-CoA