4k5b - Revision history

OCA at 08:54, 20 March 2024

OCA — Wed, 20 Mar 2024 08:54:26 GMT

←Older revision		Revision as of 08:54, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k5b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K5B FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k5b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K5B FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5b OCA], [https://pdbe.org/4k5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k5b RCSB], [https://www.ebi.ac.uk/pdbsum/4k5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k5b ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5b OCA], [https://pdbe.org/4k5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k5b RCSB], [https://www.ebi.ac.uk/pdbsum/4k5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k5b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/B2CL2_BOVIN B2CL2_BOVIN] Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX (By similarity).		[https://www.uniprot.org/uniprot/B2CL2_BOVIN B2CL2_BOVIN] Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX (By similarity).
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BCL-W is a member of the BCL-2 family of anti-apoptotic proteins. A key event in the regulation of apoptosis is the heterodimerization between anti-apoptotic and pro-apoptotic family members, which involves a conserved surface-exposed groove on the anti-apoptotic proteins. Crystal structures of the ligand binding-competent conformation exist for all anti-apoptotic family members, with the exception of BCL-W, due to the flexibility of the BCL-W groove region. Existing structures had suggested major deviations of the BCL-W groove region from the otherwise structurally highly related remaining anti-apoptotic family members. To capture its ligand binding-competent conformation by counteracting the conformational flexibility of the BCL-W groove, we had selected high-affinity groove-binding designed ankyrin repeat proteins (DARPins) using ribosome-display. We now determined two high-resolution crystal structures of human BCL-W in complex with different DARPins at a resolution of 1.5 and 1.85A, in which the structure of BCL-W is virtually identical, and in both structures BCL-W adopts a conformation extremely similar to the ligand-free conformation of its closest relative BCL-XL. However, distinct differences to all previous BCL-W structures are evident, notably in the ligand-binding region. We provide the first structural explanation for the conformational flexibility of the BCL-W groove region in comparison to other BCL-2 family members. Due to the importance of the anti-apoptotic BCL-2 family as drug targets, the presented crystal structure of ligand binding-competent BCL-W may serve as a valuable basis for structure-based drug design in the future and provides a missing piece for the structural characterization of this protein family.
-
-	Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W.,Schilling J, Schoppe J, Sauer E, Pluckthun A J Mol Biol. 2014 Apr 17. pii: S0022-2836(14)00194-6. doi:, 10.1016/j.jmb.2014.04.010. PMID:24747052<ref>PMID:24747052</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4k5b" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[B-cell lymphoma proteins 3D structures\|B-cell lymphoma proteins 3D structures]]		*[[B-cell lymphoma proteins 3D structures\|B-cell lymphoma proteins 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:40, 30 November 2022

OCA — Wed, 30 Nov 2022 11:40:42 GMT

←Older revision		Revision as of 11:40, 30 November 2022
Line 1:		Line 1:

	==Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W==		==Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W==
-	<StructureSection load='4k5b' size='340' side='right' caption='[[4k5b]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='4k5b' size='340' side='right'caption='[[4k5b]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4k5b]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus coli" migula 1895~~] and [~~http~~://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4K5B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4k5b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K5B FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k5a\|4k5a]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5b OCA], [https://pdbe.org/4k5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k5b RCSB], [https://www.ebi.ac.uk/pdbsum/4k5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k5b ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCL2L2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4k5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5b OCA], [~~http~~://pdbe.org/4k5b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4k5b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4k5b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4k5b ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/B2CL2_BOVIN B2CL2_BOVIN]] Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX (By similarity).	+	[https://www.uniprot.org/uniprot/B2CL2_BOVIN B2CL2_BOVIN] Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 4k5b" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4k5b" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[B-cell lymphoma proteins 3D structures\|B-cell lymphoma proteins 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Bos taurus]]
-	[[Category: ~~Bovin~~]]	+	[[Category: Escherichia coli]]
-	[[Category: Plueckthun, A]]	+	[[Category: Large Structures]]
-	[[Category: Sauer, E]]	+	[[Category: Plueckthun A]]
-	[[Category: Schilling, J]]	+	[[Category: Sauer E]]
-	[[Category: Schoeppe, J]]	+	[[Category: Schilling J]]
-	~~[[Category: Anti-apoptotic bcl-2 family]]~~	+	[[Category: Schoeppe J]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Bcl-w]]~~	+
-	~~[[Category: Crystal structure]]~~	+
-	~~[[Category: Darpin]]~~	+
-	~~[[Category: Ligand binding-competent conformation~~]]	+

OCA at 18:57, 5 August 2016

OCA — Fri, 05 Aug 2016 18:57:29 GMT

←Older revision		Revision as of 18:57, 5 August 2016
Line 1:		Line 1:
		+
	==Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W==		==Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W==
	<StructureSection load='4k5b' size='340' side='right' caption='[[4k5b]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='4k5b' size='340' side='right' caption='[[4k5b]], [[Resolution\|resolution]] 1.85Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k5a\|4k5a]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k5a\|4k5a]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCL2L2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCL2L2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k5b RCSB], [http://www.ebi.ac.uk/pdbsum/4k5b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5b OCA], [http://pdbe.org/4k5b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k5b RCSB], [http://www.ebi.ac.uk/pdbsum/4k5b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k5b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4k5b" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:01, 25 December 2014

OCA — Thu, 25 Dec 2014 05:01:12 GMT

←Older revision		Revision as of 05:01, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k5b]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K5B FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k5b]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K5B FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k5a\|4k5a]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k5a\|4k5a]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCL2L2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCL2L2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k5b RCSB], [http://www.ebi.ac.uk/pdbsum/4k5b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k5b RCSB], [http://www.ebi.ac.uk/pdbsum/4k5b PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/B2CL2_BOVIN B2CL2_BOVIN]] Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Bovin]]		[[Category: Bovin]]
-	[[Category: Plueckthun, A.]]	+	[[Category: Plueckthun, A]]
-	[[Category: Sauer, E.]]	+	[[Category: Sauer, E]]
-	[[Category: Schilling, J.]]	+	[[Category: Schilling, J]]
-	[[Category: Schoeppe, J.]]	+	[[Category: Schoeppe, J]]
	[[Category: Anti-apoptotic bcl-2 family]]		[[Category: Anti-apoptotic bcl-2 family]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]

OCA at 06:30, 11 June 2014

OCA — Wed, 11 Jun 2014 06:30:39 GMT

←Older revision		Revision as of 06:30, 11 June 2014
Line 2:		Line 2:
	<StructureSection load='4k5b' size='340' side='right' caption='[[4k5b]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='4k5b' size='340' side='right' caption='[[4k5b]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4k5b]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5B OCA]. <br>	+	<table><tr><td colspan='2'>[[4k5b]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K5B FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k5a\|4k5a]]</td></tr>		</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k5a\|4k5a]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCL2L2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>		<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCL2L2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k5b RCSB], [http://www.ebi.ac.uk/pdbsum/4k5b PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k5b RCSB], [http://www.ebi.ac.uk/pdbsum/4k5b PDBsum]</span></td></tr>
	<table>		<table>
Line 14:		Line 13:
	Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W.,Schilling J, Schoppe J, Sauer E, Pluckthun A J Mol Biol. 2014 Apr 17. pii: S0022-2836(14)00194-6. doi:, 10.1016/j.jmb.2014.04.010. PMID:24747052<ref>PMID:24747052</ref>		Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W.,Schilling J, Schoppe J, Sauer E, Pluckthun A J Mol Biol. 2014 Apr 17. pii: S0022-2836(14)00194-6. doi:, 10.1016/j.jmb.2014.04.010. PMID:24747052<ref>PMID:24747052</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 06:50, 7 May 2014

OCA — Wed, 07 May 2014 06:50:14 GMT

←Older revision		Revision as of 06:50, 7 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4k5b\| PDB=4k5b \| SCENE= }}~~	+	==Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W==
-	===Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W===	+	<StructureSection load='4k5b' size='340' side='right' caption='[[4k5b]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4k5b]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5B OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k5a\|4k5a]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCL2L2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k5b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k5b RCSB], [http://www.ebi.ac.uk/pdbsum/4k5b PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BCL-W is a member of the BCL-2 family of anti-apoptotic proteins. A key event in the regulation of apoptosis is the heterodimerization between anti-apoptotic and pro-apoptotic family members, which involves a conserved surface-exposed groove on the anti-apoptotic proteins. Crystal structures of the ligand binding-competent conformation exist for all anti-apoptotic family members, with the exception of BCL-W, due to the flexibility of the BCL-W groove region. Existing structures had suggested major deviations of the BCL-W groove region from the otherwise structurally highly related remaining anti-apoptotic family members. To capture its ligand binding-competent conformation by counteracting the conformational flexibility of the BCL-W groove, we had selected high-affinity groove-binding designed ankyrin repeat proteins (DARPins) using ribosome-display. We now determined two high-resolution crystal structures of human BCL-W in complex with different DARPins at a resolution of 1.5 and 1.85A, in which the structure of BCL-W is virtually identical, and in both structures BCL-W adopts a conformation extremely similar to the ligand-free conformation of its closest relative BCL-XL. However, distinct differences to all previous BCL-W structures are evident, notably in the ligand-binding region. We provide the first structural explanation for the conformational flexibility of the BCL-W groove region in comparison to other BCL-2 family members. Due to the importance of the anti-apoptotic BCL-2 family as drug targets, the presented crystal structure of ligand binding-competent BCL-W may serve as a valuable basis for structure-based drug design in the future and provides a missing piece for the structural characterization of this protein family.

-	~~==Function==~~	+	Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W.,Schilling J, Schoppe J, Sauer E, Pluckthun A J Mol Biol. 2014 Apr 17. pii: S0022-2836(14)00194-6. doi:, 10.1016/j.jmb.2014.04.010. PMID:24747052<ref>PMID:24747052</ref>
-	~~[[http://www~~.~~uniprot~~.~~org/uniprot/B2CL2_BOVIN B2CL2_BOVIN]] Promotes cell survival~~. ~~Blocks dexamethasone~~-~~induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX~~ (~~By similarity~~).	+

-	==~~About this Structure~~==	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	[[~~4k5b~~]] ~~is a 4 chain structure. Full crystallographic information is available from~~ [~~http~~:~~//oca.weizmann.ac.il/oca-bin/ocashort?id=4K5B OCA~~].	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Bovin]]
	[[Category: Plueckthun, A.]]		[[Category: Plueckthun, A.]]
	[[Category: Sauer, E.]]		[[Category: Sauer, E.]]

OCA at 10:02, 16 April 2014

OCA — Wed, 16 Apr 2014 10:02:08 GMT

←Older revision		Revision as of 10:02, 16 April 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4k5b\| PDB=4k5b \| SCENE= }}
		+	===Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W===

-	~~The entry 4k5b is ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/B2CL2_BOVIN B2CL2_BOVIN]] Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX (By similarity).

-	~~Authors~~: ~~Schilling, J~~.~~, Schoeppe, J~~., Sauer, E., ~~Plueckthun~~, A.	+	==About this Structure==
-		+	[[4k5b]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K5B OCA].
-	~~Description~~: ~~First crystal structure of anti~~-apoptotic ~~BCL~~-~~W in its closed~~ conformation	+	[[Category: Plueckthun, A.]]
		+	[[Category: Sauer, E.]]
		+	[[Category: Schilling, J.]]
		+	[[Category: Schoeppe, J.]]
		+	[[Category: Anti-apoptotic bcl-2 family]]
		+	[[Category: Apoptosis]]
		+	[[Category: Bcl-w]]
		+	[[Category: Crystal structure]]
		+	[[Category: Darpin]]
		+	[[Category: Ligand binding-competent conformation]]

OCA at 07:17, 8 May 2013

OCA — Wed, 08 May 2013 07:17:47 GMT

←Older revision		Revision as of 07:17, 8 May 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4k5b is ON HOLD	+	The entry 4k5b is ON HOLD until Paper Publication

-	Authors: Schilling, J., Schoeppe, J., Sauer, E.	+	Authors: Schilling, J., Schoeppe, J., Sauer, E., Plueckthun, A.

	Description: First crystal structure of anti-apoptotic BCL-W in its closed conformation		Description: First crystal structure of anti-apoptotic BCL-W in its closed conformation

OCA: Protected "4k5b" [edit=sysop:move=sysop]

OCA — Wed, 24 Apr 2013 10:59:40 GMT

Protected "4k5b" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:59, 24 April 2013

OCA: New page: '''Unreleased structure''' The entry 4k5b is ON HOLD Authors: Schilling, J., Schoeppe, J., Sauer, E. Description: First crystal structure of anti-apoptotic BCL-W in its closed conforma...

OCA — Wed, 24 Apr 2013 10:59:39 GMT

New page: '''Unreleased structure''' The entry 4k5b is ON HOLD Authors: Schilling, J., Schoeppe, J., Sauer, E. Description: First crystal structure of anti-apoptotic BCL-W in its closed conforma...

New page

'''Unreleased structure'''

The entry 4k5b is ON HOLD

Authors: Schilling, J., Schoeppe, J., Sauer, E.

Description: First crystal structure of anti-apoptotic BCL-W in its closed conformation