4k9b - Revision history

OCA at 19:23, 29 May 2024

OCA — Wed, 29 May 2024 19:23:54 GMT

←Older revision		Revision as of 19:23, 29 May 2024
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[4k9b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K9B FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k9b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K9B FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.26Å</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.26Å</td></tr>
-	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k9b OCA], [https://pdbe.org/4k9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k9b RCSB], [https://www.ebi.ac.uk/pdbsum/4k9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k9b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k9b OCA], [https://pdbe.org/4k9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k9b RCSB], [https://www.ebi.ac.uk/pdbsum/4k9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k9b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CGAS_MOUSE CGAS_MOUSE] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.		[https://www.uniprot.org/uniprot/CGAS_MOUSE CGAS_MOUSE] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Recent studies identified cyclic GMP-AMP (cGAMP) as a metazoan second messenger triggering an interferon response. cGAMP is generated from GTP and ATP by cytoplasmic dsDNA sensor cGAMP synthase (cGAS). We combined structural, chemical, biochemical, and cellular assays to demonstrate that this second messenger contains G(2',5')pA and A(3',5')pG phosphodiester linkages, designated c[G(2',5')pA(3',5')p]. We show that, upon dsDNA binding, cGAS is activated through conformational transitions, resulting in formation of a catalytically competent and accessible nucleotide-binding pocket for generation of c[G(2',5')pA(3',5')p]. We demonstrate that cyclization occurs in a stepwise manner through initial generation of 5'-pppG(2',5')pA prior to cyclization to c[G(2',5')pA(3',5')p], with the latter positioned precisely in the catalytic pocket. Mutants of cGAS dsDNA-binding or catalytic pocket residues exhibit reduced or abrogated activity. Our studies have identified c[G(2',5')pA(3',5')p] as a founding member of a family of metazoan 2',5'-containing cyclic heterodinucleotide second messengers distinct from bacterial 3',5' cyclic dinucleotides.
		+
		+	Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase.,Gao P, Ascano M, Wu Y, Barchet W, Gaffney BL, Zillinger T, Serganov AA, Liu Y, Jones RA, Hartmann G, Tuschl T, Patel DJ Cell. 2013 May 23;153(5):1094-107. doi: 10.1016/j.cell.2013.04.046. Epub 2013 May, 3. PMID:23647843<ref>PMID:23647843</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4k9b" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Cyclic GMP-AMP synthase 3D synthase\|Cyclic GMP-AMP synthase 3D synthase]]		*[[Cyclic GMP-AMP synthase 3D synthase\|Cyclic GMP-AMP synthase 3D synthase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:09, 1 March 2024

OCA — Fri, 01 Mar 2024 12:09:38 GMT

←Older revision		Revision as of 12:09, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k9b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K9B FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k9b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K9B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.26Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k9b OCA], [https://pdbe.org/4k9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k9b RCSB], [https://www.ebi.ac.uk/pdbsum/4k9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k9b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k9b OCA], [https://pdbe.org/4k9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k9b RCSB], [https://www.ebi.ac.uk/pdbsum/4k9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k9b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CGAS_MOUSE CGAS_MOUSE] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.		[https://www.uniprot.org/uniprot/CGAS_MOUSE CGAS_MOUSE] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Recent studies identified cyclic GMP-AMP (cGAMP) as a metazoan second messenger triggering an interferon response. cGAMP is generated from GTP and ATP by cytoplasmic dsDNA sensor cGAMP synthase (cGAS). We combined structural, chemical, biochemical, and cellular assays to demonstrate that this second messenger contains G(2',5')pA and A(3',5')pG phosphodiester linkages, designated c[G(2',5')pA(3',5')p]. We show that, upon dsDNA binding, cGAS is activated through conformational transitions, resulting in formation of a catalytically competent and accessible nucleotide-binding pocket for generation of c[G(2',5')pA(3',5')p]. We demonstrate that cyclization occurs in a stepwise manner through initial generation of 5'-pppG(2',5')pA prior to cyclization to c[G(2',5')pA(3',5')p], with the latter positioned precisely in the catalytic pocket. Mutants of cGAS dsDNA-binding or catalytic pocket residues exhibit reduced or abrogated activity. Our studies have identified c[G(2',5')pA(3',5')p] as a founding member of a family of metazoan 2',5'-containing cyclic heterodinucleotide second messengers distinct from bacterial 3',5' cyclic dinucleotides.
-
-	Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase.,Gao P, Ascano M, Wu Y, Barchet W, Gaffney BL, Zillinger T, Serganov AA, Liu Y, Jones RA, Hartmann G, Tuschl T, Patel DJ Cell. 2013 May 23;153(5):1094-107. doi: 10.1016/j.cell.2013.04.046. Epub 2013 May, 3. PMID:23647843<ref>PMID:23647843</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4k9b" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Cyclic GMP-AMP synthase 3D synthase\|Cyclic GMP-AMP synthase 3D synthase]]		*[[Cyclic GMP-AMP synthase 3D synthase\|Cyclic GMP-AMP synthase 3D synthase]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:46, 30 November 2022

OCA — Wed, 30 Nov 2022 11:46:49 GMT

←Older revision		Revision as of 11:46, 30 November 2022
Line 1:		Line 1:

	==Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p]==		==Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p]==
-	<StructureSection load='4k9b' size='340' side='right' caption='[[4k9b]], [[Resolution\|resolution]] 2.26Å' scene=''>	+	<StructureSection load='4k9b' size='340' side='right'caption='[[4k9b]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4k9b]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4K9B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4k9b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K9B FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Mb21d1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k9b OCA], [https://pdbe.org/4k9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k9b RCSB], [https://www.ebi.ac.uk/pdbsum/4k9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k9b ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k9b OCA], [~~http~~://pdbe.org/4k9b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4k9b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4k9b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4k9b ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CGAS_MOUSE CGAS_MOUSE]] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.	+	[https://www.uniprot.org/uniprot/CGAS_MOUSE CGAS_MOUSE] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
-	*[[Cyclic GMP-AMP synthase\|Cyclic GMP-AMP synthase]]	+	*[[Cyclic GMP-AMP synthase 3D synthase\|Cyclic GMP-AMP synthase 3D synthase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Gao, P~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Patel, D J~~]]	+	[[Category: Gao P]]
-	[[Category: ~~Wu, Y~~]]	+	[[Category: Patel DJ]]
-	[[Category: ~~Nucleotidyltransferase fold]]~~	+	[[Category: Wu Y]]
-	~~[[Category: Transferase-dna complex~~]]	+

OCA at 13:45, 5 August 2016

OCA — Fri, 05 Aug 2016 13:45:50 GMT

←Older revision		Revision as of 13:45, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p]==		==Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p]==
	<StructureSection load='4k9b' size='340' side='right' caption='[[4k9b]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='4k9b' size='340' side='right' caption='[[4k9b]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4k9b]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K9B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4k9b]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K9B FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Mb21d1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Mb21d1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k9b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k9b RCSB], [http://www.ebi.ac.uk/pdbsum/4k9b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k9b OCA], [http://pdbe.org/4k9b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k9b RCSB], [http://www.ebi.ac.uk/pdbsum/4k9b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k9b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4k9b" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Gao, P]]		[[Category: Gao, P]]
	[[Category: Patel, D J]]		[[Category: Patel, D J]]

OCA at 19:18, 25 December 2014

OCA — Thu, 25 Dec 2014 19:18:15 GMT

←Older revision		Revision as of 19:18, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k9b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k9b RCSB], [http://www.ebi.ac.uk/pdbsum/4k9b PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k9b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k9b RCSB], [http://www.ebi.ac.uk/pdbsum/4k9b PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CGAS_MOUSE CGAS_MOUSE]] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 14:52, 21 December 2014

OCA — Sun, 21 Dec 2014 14:52:48 GMT

←Older revision		Revision as of 14:52, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4k9b\| PDB=4k9b \| SCENE= }}~~	+	==Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p]==
-	===Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p]===	+	<StructureSection load='4k9b' size='340' side='right' caption='[[4k9b]], [[Resolution\|resolution]] 2.26Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23647843}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4k9b]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K9B FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Mb21d1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k9b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k9b RCSB], [http://www.ebi.ac.uk/pdbsum/4k9b PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Recent studies identified cyclic GMP-AMP (cGAMP) as a metazoan second messenger triggering an interferon response. cGAMP is generated from GTP and ATP by cytoplasmic dsDNA sensor cGAMP synthase (cGAS). We combined structural, chemical, biochemical, and cellular assays to demonstrate that this second messenger contains G(2',5')pA and A(3',5')pG phosphodiester linkages, designated c[G(2',5')pA(3',5')p]. We show that, upon dsDNA binding, cGAS is activated through conformational transitions, resulting in formation of a catalytically competent and accessible nucleotide-binding pocket for generation of c[G(2',5')pA(3',5')p]. We demonstrate that cyclization occurs in a stepwise manner through initial generation of 5'-pppG(2',5')pA prior to cyclization to c[G(2',5')pA(3',5')p], with the latter positioned precisely in the catalytic pocket. Mutants of cGAS dsDNA-binding or catalytic pocket residues exhibit reduced or abrogated activity. Our studies have identified c[G(2',5')pA(3',5')p] as a founding member of a family of metazoan 2',5'-containing cyclic heterodinucleotide second messengers distinct from bacterial 3',5' cyclic dinucleotides.

-	~~==Function==~~	+	Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase.,Gao P, Ascano M, Wu Y, Barchet W, Gaffney BL, Zillinger T, Serganov AA, Liu Y, Jones RA, Hartmann G, Tuschl T, Patel DJ Cell. 2013 May 23;153(5):1094-107. doi: 10.1016/j.cell.2013.04.046. Epub 2013 May, 3. PMID:23647843<ref>PMID:23647843</ref>
-	[~~[http://www.uniprot.org/uniprot/CGAS_MOUSE CGAS_MOUSE~~]~~] Nucleotidyltransferase that catalyzes formation of~~ cyclic GMP-AMP ~~(cGAMP) from ATP and GTP and exhibits antiviral activity~~. ~~Has antiviral activity by acting as a key cytosolic DNA sensor~~, ~~the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly~~, ~~leading to activation and synthesis of cGAMP~~, ~~a second messenger that binds to and activates TMEM173/STING~~, ~~thereby triggering type~~-~~I interferon production~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4k9b]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:023647843</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Cyclic GMP-AMP synthase\|Cyclic GMP-AMP synthase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Gao, P.]]	+	[[Category: Gao, P]]
-	[[Category: Patel, D J.]]	+	[[Category: Patel, D J]]
-	[[Category: Wu, Y.]]	+	[[Category: Wu, Y]]
	[[Category: Nucleotidyltransferase fold]]		[[Category: Nucleotidyltransferase fold]]
	[[Category: Transferase-dna complex]]		[[Category: Transferase-dna complex]]

OCA at 05:04, 28 August 2013

OCA — Wed, 28 Aug 2013 05:04:44 GMT

←Older revision		Revision as of 05:04, 28 August 2013
Line 1:		Line 1:
	{{STRUCTURE_4k9b\| PDB=4k9b \| SCENE= }}		{{STRUCTURE_4k9b\| PDB=4k9b \| SCENE= }}
	===Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p]===		===Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p]===
		+	{{ABSTRACT_PUBMED_23647843}}

	==Function==		==Function==
Line 7:		Line 8:
	==About this Structure==		==About this Structure==
	[[4k9b]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA].		[[4k9b]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023647843</ref><references group="xtra"/><references/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Gao, P.]]		[[Category: Gao, P.]]

OCA at 07:34, 16 May 2013

OCA — Thu, 16 May 2013 07:34:14 GMT

←Older revision		Revision as of 07:34, 16 May 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4k9b\| PDB=4k9b \| SCENE= }}
		+	===Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p]===

-	~~The entry 4k9b is ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/CGAS_MOUSE CGAS_MOUSE]] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.

-	~~Authors~~: Gao, P.~~, Wu, Y.,~~ Patel, D.J.	+	==About this Structure==
-		+	[[4k9b]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K9B OCA].
-	~~Description~~: ~~Structure of Ternary Complex of cGAS with dsDNA and Bound c~~[~~G(2 ,5 )pA(3 ,5 )p~~]	+	[[Category: Mus musculus]]
		+	[[Category: Gao, P.]]
		+	[[Category: Patel, D J.]]
		+	[[Category: Wu, Y.]]
		+	[[Category: Nucleotidyltransferase fold]]
		+	[[Category: Transferase-dna complex]]

OCA at 07:19, 8 May 2013

OCA — Wed, 08 May 2013 07:19:00 GMT

←Older revision		Revision as of 07:19, 8 May 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4k9b is ON HOLD ~~until sometime in the future~~	+	The entry 4k9b is ON HOLD

	Authors: Gao, P., Wu, Y., Patel, D.J.		Authors: Gao, P., Wu, Y., Patel, D.J.

-	Description: ~~A new protein structure 7~~	+	Description: Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p]

OCA: Protected "4k9b" [edit=sysop:move=sysop]

OCA — Thu, 02 May 2013 07:12:58 GMT

Protected "4k9b" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:12, 2 May 2013