4ker - Revision history

OCA at 11:54, 30 November 2022

OCA — Wed, 30 Nov 2022 11:54:52 GMT

←Older revision		Revision as of 11:54, 30 November 2022
Line 1:		Line 1:

	==Crystal structure of SsoPox W263V==		==Crystal structure of SsoPox W263V==
-	<StructureSection load='4ker' size='340' side='right' caption='[[4ker]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='4ker' size='340' side='right'caption='[[4ker]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ker]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_35091 Atcc 35091~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KER OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4KER FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ker]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KER FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, ~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ker FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ker OCA], [https://pdbe.org/4ker PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ker RCSB], [https://www.ebi.ac.uk/pdbsum/4ker PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ker ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vc5\|2vc5]]~~, ~~[[2vc7\|2vc7]], [[3uf9\|3uf9]], [[4kes\|4kes]], [[4ket\|4ket]], [[4keu\|4keu]], [[4kev\|4kev]], [[4kez\|4kez]], [[4kf1\|4kf1]]~~<~~/td></tr>~~	+
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">php, php SSO2522, SSO2522~~ (~~[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091]~~)~~</td></tr>~~	+
-	~~<tr id='activity~~'>~~<td class="sblockLbl"><b>Activity:~~</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryldialkylphosphatase Aryldialkylphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.1 3.1.8.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ker FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ker OCA], [~~http~~://pdbe.org/4ker PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ker RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ker PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ker ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~PHP_SULSO PHP_SULSO]~~] Has a low paraoxonase activity. Also active, but with a lower activity, against other ~~oregano~~-phosphorus insecticides such as Dursban, Coumaphos, pNP-butanoate or parathion.<ref>PMID:15909078</ref>	+	[https://www.uniprot.org/uniprot/PHP_SACS2 PHP_SACS2] Has a low paraoxonase activity. Also active, but with a lower activity, against other organo-phosphorus insecticides such as Dursban, Coumaphos, pNP-butanoate or parathion.<ref>PMID:15909078</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 20:

	==See Also==		==See Also==
-	*[[Phosphotriesterase\|Phosphotriesterase]]	+	*[[Phosphotriesterase 3D structures\|Phosphotriesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aryldialkylphosphatase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Atcc 35091~~]]	+	[[Category: Saccharolobus solfataricus]]
-	[[Category: Chabriere, E]]	+	[[Category: Chabriere E]]
-	[[Category: Elias, M]]	+	[[Category: Elias M]]
-	[[Category: Gotthard, G]]	+	[[Category: Gotthard G]]
-	[[Category: Hiblot, J]]	+	[[Category: Hiblot J]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lactonase~~]]	+

OCA at 22:47, 5 August 2016

OCA — Fri, 05 Aug 2016 22:47:30 GMT

←Older revision		Revision as of 22:47, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of SsoPox W263V==		==Crystal structure of SsoPox W263V==
	<StructureSection load='4ker' size='340' side='right' caption='[[4ker]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4ker' size='340' side='right' caption='[[4ker]], [[Resolution\|resolution]] 2.60Å' scene=''>
Line 8:		Line 9:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">php, php SSO2522, SSO2522 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">php, php SSO2522, SSO2522 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryldialkylphosphatase Aryldialkylphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.1 3.1.8.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryldialkylphosphatase Aryldialkylphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.1 3.1.8.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ker FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ker OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ker RCSB], [http://www.ebi.ac.uk/pdbsum/4ker PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ker FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ker OCA], [http://pdbe.org/4ker PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ker RCSB], [http://www.ebi.ac.uk/pdbsum/4ker PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ker ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 21:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ker" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphotriesterase\|Phosphotriesterase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:31, 25 December 2014

OCA — Thu, 25 Dec 2014 06:31:46 GMT

←Older revision		Revision as of 06:31, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4ker\| PDB~~=4ker \| ~~SCENE~~= }}	+	==Crystal structure of SsoPox W263V==
-	===~~Crystal~~ structure ~~of SsoPox W263V~~===	+	<StructureSection load='4ker' size='340' side='right' caption='[[4ker]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24086491}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4ker]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KER OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KER FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vc5\|2vc5]], [[2vc7\|2vc7]], [[3uf9\|3uf9]], [[4kes\|4kes]], [[4ket\|4ket]], [[4keu\|4keu]], [[4kev\|4kev]], [[4kez\|4kez]], [[4kf1\|4kf1]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">php, php SSO2522, SSO2522 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryldialkylphosphatase Aryldialkylphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.1 3.1.8.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ker FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ker OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ker RCSB], [http://www.ebi.ac.uk/pdbsum/4ker PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/PHP_SULSO PHP_SULSO]] Has a low paraoxonase activity. Also active, but with a lower activity, against other oregano-phosphorus insecticides such as Dursban, Coumaphos, pNP-butanoate or parathion.<ref>PMID:15909078</ref>		[[http://www.uniprot.org/uniprot/PHP_SULSO PHP_SULSO]] Has a low paraoxonase activity. Also active, but with a lower activity, against other oregano-phosphorus insecticides such as Dursban, Coumaphos, pNP-butanoate or parathion.<ref>PMID:15909078</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enzymes are proficient catalysts that enable fast rates of Michaelis-complex formation, the chemical step and products release. These different steps may require different conformational states of the active site that have distinct binding properties. Moreover, the conformational flexibility of the active site mediates alternative, promiscuous functions. Here we focused on the lactonase SsoPox from Sulfolobus solfataricus. SsoPox is a native lactonase endowed with promiscuous phosphotriesterase activity. We identified a position in the active site loop (W263) that governs its flexibility, and thereby affects the substrate specificity of the enzyme. We isolated two different sets of substitutions at position 263 that induce two distinct conformational sampling of the active loop and characterized the structural and kinetic effects of these substitutions. These sets of mutations selectively and distinctly mediate the improvement of the promiscuous phosphotriesterase and oxo-lactonase activities of SsoPox by increasing active-site loop flexibility. These observations corroborate the idea that conformational diversity governs enzymatic promiscuity and is a key feature of protein evolvability.

-	~~==About this Structure==~~	+	Differential Active Site Loop Conformations Mediate Promiscuous Activities in the Lactonase SsoPox.,Hiblot J, Gotthard G, Elias M, Chabriere E PLoS One. 2013 Sep 23;8(9):e75272. doi: 10.1371/journal.pone.0075272. PMID:24086491<ref>PMID:24086491</ref>
-	~~[[4ker]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Atcc_35091 Atcc 35091]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4KER OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024086491</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Aryldialkylphosphatase]]		[[Category: Aryldialkylphosphatase]]
	[[Category: Atcc 35091]]		[[Category: Atcc 35091]]
-	[[Category: Chabriere, E.]]	+	[[Category: Chabriere, E]]
-	[[Category: Elias, M.]]	+	[[Category: Elias, M]]
-	[[Category: Gotthard, G.]]	+	[[Category: Gotthard, G]]
-	[[Category: Hiblot, J.]]	+	[[Category: Hiblot, J]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Lactonase]]		[[Category: Lactonase]]

OCA at 12:23, 20 November 2013

OCA — Wed, 20 Nov 2013 12:23:24 GMT

←Older revision		Revision as of 12:23, 20 November 2013
Line 1:		Line 1:
	{{STRUCTURE_4ker\| PDB=4ker \| SCENE= }}		{{STRUCTURE_4ker\| PDB=4ker \| SCENE= }}
	===Crystal structure of SsoPox W263V===		===Crystal structure of SsoPox W263V===
		+	{{ABSTRACT_PUBMED_24086491}}

	==Function==		==Function==
Line 6:		Line 7:

	==About this Structure==		==About this Structure==
-	[[4ker]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus Sulfolobus solfataricus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KER OCA].	+	[[4ker]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KER OCA].

	==Reference==		==Reference==
-	<references group="xtra"/><references/>	+	<ref group="xtra">PMID:024086491</ref><references group="xtra"/><references/>
	[[Category: Aryldialkylphosphatase]]		[[Category: Aryldialkylphosphatase]]
-	[[Category: ~~Sulfolobus solfataricus~~]]	+	[[Category: Atcc 35091]]
	[[Category: Chabriere, E.]]		[[Category: Chabriere, E.]]
	[[Category: Elias, M.]]		[[Category: Elias, M.]]

OCA at 07:35, 2 October 2013

OCA — Wed, 02 Oct 2013 07:35:36 GMT

←Older revision		Revision as of 07:35, 2 October 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4ker\| PDB=4ker \| SCENE= }}
		+	===Crystal structure of SsoPox W263V===

-	~~The entry 4ker is ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/PHP_SULSO PHP_SULSO]] Has a low paraoxonase activity. Also active, but with a lower activity, against other oregano-phosphorus insecticides such as Dursban, Coumaphos, pNP-butanoate or parathion.<ref>PMID:15909078</ref>

-	~~Authors~~: ~~Gotthard, G~~.~~, Hiblot, J~~.~~, Chabriere, E~~.~~, Elias, M~~.	+	==About this Structure==
		+	[[4ker]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KER OCA].

-	~~Description~~: ~~Crystal structure of SsoPox W263V~~	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Aryldialkylphosphatase]]
		+	[[Category: Sulfolobus solfataricus]]
		+	[[Category: Chabriere, E.]]
		+	[[Category: Elias, M.]]
		+	[[Category: Gotthard, G.]]
		+	[[Category: Hiblot, J.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lactonase]]

OCA at 13:10, 3 July 2013

OCA — Wed, 03 Jul 2013 13:10:58 GMT

←Older revision		Revision as of 13:10, 3 July 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ker is ON HOLD	+	The entry 4ker is ON HOLD until Paper Publication

	Authors: Gotthard, G., Hiblot, J., Chabriere, E., Elias, M.		Authors: Gotthard, G., Hiblot, J., Chabriere, E., Elias, M.

	Description: Crystal structure of SsoPox W263V		Description: Crystal structure of SsoPox W263V

OCA: Protected "4ker" [edit=sysop:move=sysop]

OCA — Wed, 08 May 2013 07:21:03 GMT

Protected "4ker" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:21, 8 May 2013

OCA: New page: '''Unreleased structure''' The entry 4ker is ON HOLD Authors: Gotthard, G., Hiblot, J., Chabriere, E., Elias, M. Description: Crystal structure of SsoPox W263V

OCA — Wed, 08 May 2013 07:21:02 GMT

New page: '''Unreleased structure''' The entry 4ker is ON HOLD Authors: Gotthard, G., Hiblot, J., Chabriere, E., Elias, M. Description: Crystal structure of SsoPox W263V

New page

'''Unreleased structure'''

The entry 4ker is ON HOLD

Authors: Gotthard, G., Hiblot, J., Chabriere, E., Elias, M.

Description: Crystal structure of SsoPox W263V