4kka - Revision history

OCA at 08:55, 7 December 2022

OCA — Wed, 07 Dec 2022 08:55:58 GMT

←Older revision		Revision as of 08:55, 7 December 2022
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	==Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide==		==Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide==
-	<StructureSection load='4kka' size='340' side='right' caption='[[4kka]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='4kka' size='340' side='right'caption='[[4kka]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kka]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~] and [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KKA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4KKA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kka]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KKA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KKA FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kjw\|4kjw]], [[4kk5\|4kk5]], [[4kk6\|4kk6]], [[4kk8\|4kk8]], [[4kk9\|4kk9]], [[4kkb\|4kkb]], [[4kkc\|4kkc]], [[4kjp\|4kjp]], [[4kjq\|4kjq]]</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kka FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kka OCA], [https://pdbe.org/4kka PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kka RCSB], [https://www.ebi.ac.uk/pdbsum/4kka PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kka ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0155, clcA, eriC, JW5012, yadQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4kka FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kka OCA], [~~http~~://pdbe.org/4kka PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4kka RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4kka PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4kka ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI]] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>	+	[https://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lim, H H~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Miller, C~~]]	+	[[Category: Lim H-H]]
-	[[Category: ~~Membrane transporter]]~~	+	[[Category: Miller C]]
-	~~[[Category: Transport protein~~]]	+

OCA at 13:31, 4 August 2016

OCA — Thu, 04 Aug 2016 13:31:39 GMT

←Older revision		Revision as of 13:31, 4 August 2016
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		+
	==Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide==		==Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide==
	<StructureSection load='4kka' size='340' side='right' caption='[[4kka]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4kka' size='340' side='right' caption='[[4kka]], [[Resolution\|resolution]] 3.00Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kjw\|4kjw]], [[4kk5\|4kk5]], [[4kk6\|4kk6]], [[4kk8\|4kk8]], [[4kk9\|4kk9]], [[4kkb\|4kkb]], [[4kkc\|4kkc]], [[4kjp\|4kjp]], [[4kjq\|4kjq]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kjw\|4kjw]], [[4kk5\|4kk5]], [[4kk6\|4kk6]], [[4kk8\|4kk8]], [[4kk9\|4kk9]], [[4kkb\|4kkb]], [[4kkc\|4kkc]], [[4kjp\|4kjp]], [[4kjq\|4kjq]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0155, clcA, eriC, JW5012, yadQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0155, clcA, eriC, JW5012, yadQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kka FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kka OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kka RCSB], [http://www.ebi.ac.uk/pdbsum/4kka PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kka FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kka OCA], [http://pdbe.org/4kka PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kka RCSB], [http://www.ebi.ac.uk/pdbsum/4kka PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kka ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4kka" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:04, 24 December 2014

OCA — Wed, 24 Dec 2014 15:04:51 GMT

←Older revision		Revision as of 15:04, 24 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4kka\| PDB=4kka \| SCENE= }}~~	+	==Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide==
-	===Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide===	+	<StructureSection load='4kka' size='340' side='right' caption='[[4kka]], [[Resolution\|resolution]] 3.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24036509}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4kka]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KKA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KKA FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kjw\|4kjw]], [[4kk5\|4kk5]], [[4kk6\|4kk6]], [[4kk8\|4kk8]], [[4kk9\|4kk9]], [[4kkb\|4kkb]], [[4kkc\|4kkc]], [[4kjp\|4kjp]], [[4kjq\|4kjq]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0155, clcA, eriC, JW5012, yadQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kka FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kka OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kka RCSB], [http://www.ebi.ac.uk/pdbsum/4kka PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI]] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>		[[http://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI]] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cl-/H+ antiporters of the CLC superfamily transport anions across biological membranes in varied physiological contexts. These proteins are weakly selective among anions commonly studied, including Cl-, Br-, I-, NO3- and SCN-, but they seem to be very selective against F-. The recent discovery of a new CLC clade of F-/H+ antiporters, which are highly selective for F- over Cl-, led us to investigate the mechanism of Cl--over-F- selectivity by a CLC Cl-/H+ antiporter, CLC-ec1. By subjecting purified CLC-ec1 to anion transport measurements, electrophysiological recording, equilibrium ligand-binding studies and X-ray crystallography, we show that F- binds in the Cl- transport pathway with affinity similar to Cl- but stalls the transport cycle. Examination of various mutant antiporters implies a 'lock-down' mechanism of F- inhibition, in which F-, by virtue of its unique hydrogen-bonding chemistry, greatly retards a proton-linked conformational change essential for the transport cycle of CLC-ec1.

-	~~==About this Structure==~~	+	Fluoride-dependent interruption of the transport cycle of a CLC Cl/H antiporter.,Lim HH, Stockbridge RB, Miller C Nat Chem Biol. 2013 Sep 15. doi: 10.1038/nchembio.1336. PMID:24036509<ref>PMID:24036509</ref>
-	~~[[4kka]] is~~ a ~~6 chain structure with sequence from [http:/~~/en.~~wikipedia~~.~~org/wiki/Ecoli Ecoli] and [http~~:/~~/en~~.~~wikipedia~~.~~org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4KKA OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024036509</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
-	[[Category: Lim, H H.]]	+	[[Category: Lim, H H]]
-	[[Category: Miller, C.]]	+	[[Category: Miller, C]]
	[[Category: Membrane transporter]]		[[Category: Membrane transporter]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 06:37, 13 November 2013

OCA — Wed, 13 Nov 2013 06:37:21 GMT

←Older revision		Revision as of 06:37, 13 November 2013
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	==About this Structure==		==About this Structure==
-	[[4kka]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~] and [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KKA OCA].	+	[[4kka]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KKA OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:024036509</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:024036509</ref><references group="xtra"/><references/>
-	[[Category: ~~Escherichia coli k-12~~]]	+	[[Category: Ecoli]]
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Lim, H H.]]		[[Category: Lim, H H.]]
	[[Category: Miller, C.]]		[[Category: Miller, C.]]
	[[Category: Membrane transporter]]		[[Category: Membrane transporter]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 05:08, 10 October 2013

OCA — Thu, 10 Oct 2013 05:08:08 GMT

←Older revision		Revision as of 05:08, 10 October 2013
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	{{STRUCTURE_4kka\| PDB=4kka \| SCENE= }}		{{STRUCTURE_4kka\| PDB=4kka \| SCENE= }}
	===Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide===		===Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide===
		+	{{ABSTRACT_PUBMED_24036509}}

	==Function==		==Function==
Line 9:		Line 10:

	==Reference==		==Reference==
-	<references group="xtra"/><references/>	+	<ref group="xtra">PMID:024036509</ref><references group="xtra"/><references/>
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 08:35, 21 August 2013

OCA — Wed, 21 Aug 2013 08:35:32 GMT

←Older revision		Revision as of 08:35, 21 August 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4kka\| PDB=4kka \| SCENE= }}
		+	===Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide===

-	~~The entry 4kka~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI]] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>

-	~~Authors~~: ~~Lim, H~~.-H.~~, Miller, C~~.	+	==About this Structure==
		+	[[4kka]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KKA OCA].

-	~~Description~~: ~~Structure of the E148A mutant of CLC~~-~~ec1 deltaNC construct in 100mM fluoride and 20mM Bromide~~	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Escherichia coli k-12]]
		+	[[Category: Mus musculus]]
		+	[[Category: Lim, H H.]]
		+	[[Category: Miller, C.]]
		+	[[Category: Membrane transporter]]
		+	[[Category: Transport protein]]

OCA at 15:00, 10 July 2013

OCA — Wed, 10 Jul 2013 15:00:58 GMT

←Older revision		Revision as of 15:00, 10 July 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4kka is ON HOLD	+	The entry 4kka is ON HOLD until Paper Publication

	Authors: Lim, H.-H., Miller, C.		Authors: Lim, H.-H., Miller, C.

	Description: Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide		Description: Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide

OCA: Protected "4kka" [edit=sysop:move=sysop]

OCA — Thu, 16 May 2013 06:57:41 GMT

Protected "4kka" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:57, 16 May 2013

OCA: New page: '''Unreleased structure''' The entry 4kka is ON HOLD Authors: Lim, H.-H., Miller, C. Description: Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM ...

OCA — Thu, 16 May 2013 06:57:40 GMT

New page: '''Unreleased structure''' The entry 4kka is ON HOLD Authors: Lim, H.-H., Miller, C. Description: Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM ...

New page

'''Unreleased structure'''

The entry 4kka is ON HOLD

Authors: Lim, H.-H., Miller, C.

Description: Structure of the E148A mutant of CLC-ec1 deltaNC construct in 100mM fluoride and 20mM Bromide