4ktp - Revision history

OCA at 08:54, 20 March 2024

OCA — Wed, 20 Mar 2024 08:54:51 GMT

←Older revision		Revision as of 08:54, 20 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ktp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_selenitireducens_MLS10 Bacillus selenitireducens MLS10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KTP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ktp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_selenitireducens_MLS10 Bacillus selenitireducens MLS10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KTP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [https://pdbe.org/4ktp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [https://www.ebi.ac.uk/pdbsum/4ktp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ktp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [https://pdbe.org/4ktp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [https://www.ebi.ac.uk/pdbsum/4ktp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ktp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GGP_BACIE GGP_BACIE] Catalyzes both the (1) reversible phosphorolysis of 2-O-alpha-D-glucopyranosyl-sn-glycerol (GG) from beta-D-glucose 1-phosphate (betaGlc1P) and glycerol and (2) the hydrolysis of betaGlc1P. the betaGlc1P hydrolysis is a glucosyl-transfer reaction to an acceptor water molecule that produces an anomer-inverted alpha-glucose, not a phosphatase-type reaction. In the absence of glycerol produces alpha-D-glucopyranose and phosphate from beta-D-glucopyranose 1-phosphate.<ref>PMID:24466148</ref> <ref>PMID:24828502</ref>		[https://www.uniprot.org/uniprot/GGP_BACIE GGP_BACIE] Catalyzes both the (1) reversible phosphorolysis of 2-O-alpha-D-glucopyranosyl-sn-glycerol (GG) from beta-D-glucose 1-phosphate (betaGlc1P) and glycerol and (2) the hydrolysis of betaGlc1P. the betaGlc1P hydrolysis is a glucosyl-transfer reaction to an acceptor water molecule that produces an anomer-inverted alpha-glucose, not a phosphatase-type reaction. In the absence of glycerol produces alpha-D-glucopyranose and phosphate from beta-D-glucopyranose 1-phosphate.<ref>PMID:24466148</ref> <ref>PMID:24828502</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	2-O-alpha-Glucosylglycerol phosphorylase (GGP) from Bacillus selenitireducens catalyzes both the reversible phosphorolysis of 2-O-alpha-glucosylglycerol (GG) and the hydrolysis of beta-D-glucose 1-phosphate (betaGlc1P). GGP belongs to the glycoside hydrolase (GH) family 65 and can efficiently and specifically produce GG. However, its structural basis has remained unclear. In this study, the crystal structures of GGP complexed with glucose and with the glucose analogue isofagomine and glycerol were determined. Subsite -1 of GGP is similar to those of other GH65 enzymes, maltose phosphorylase and kojibiose phosphorylase, whereas subsite +1 is largely different and is well designed for GG recognition. An automated docking analysis was performed to complement these crystal structures, betaGlc1P being docked at an appropriate position. To investigate the importance of residues at subsite +1 in the bifunctionality of GGP, we constructed mutants at these residues. Y327F and K587A did not show detectable activities for either reverse phosphorolysis or betaGlc1P hydrolysis. Y572F also showed significantly reduced activities for both of these reactions. In contrast, W381F showed significantly reduced reverse phosphorolytic activity but retained betaGlc1P hydrolysis. The mode of substrate recognition and the reaction mechanisms of GGP were proposed based on these analyses. Specifically, an extensive hydrogen bond network formed by Tyr-327, Tyr-572, Lys-587, and water molecules contributes to fixing the acceptor molecule in both reverse phosphorolysis (glycerol) and betaGlc1P hydrolysis (water) for a glycosyl transfer reaction. This study will contribute to the development of a large-scale production system of GG by facilitating the rational engineering of GGP.
-
-	Structural basis for reversible phosphorolysis and hydrolysis reactions of 2-O-alpha-Glucosylglycerol phosphorylase.,Touhara KK, Nihira T, Kitaoka M, Nakai H, Fushinobu S J Biol Chem. 2014 May 14. pii: jbc.M114.573212. PMID:24828502<ref>PMID:24828502</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4ktp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:13, 7 December 2022

OCA — Wed, 07 Dec 2022 09:13:10 GMT

←Older revision		Revision as of 09:13, 7 December 2022
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	<StructureSection load='4ktp' size='340' side='right'caption='[[4ktp]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4ktp' size='340' side='right'caption='[[4ktp]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ktp]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacie Bacie~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KTP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4KTP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ktp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_selenitireducens_MLS10 Bacillus selenitireducens MLS10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KTP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ktr\|4ktr]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [https://pdbe.org/4ktp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [https://www.ebi.ac.uk/pdbsum/4ktp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ktp ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Bsel_2816 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=439292 BACIE])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [~~http~~://pdbe.org/4ktp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ktp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ktp ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GGP_BACIE GGP_BACIE] Catalyzes both the (1) reversible phosphorolysis of 2-O-alpha-D-glucopyranosyl-sn-glycerol (GG) from beta-D-glucose 1-phosphate (betaGlc1P) and glycerol and (2) the hydrolysis of betaGlc1P. the betaGlc1P hydrolysis is a glucosyl-transfer reaction to an acceptor water molecule that produces an anomer-inverted alpha-glucose, not a phosphatase-type reaction. In the absence of glycerol produces alpha-D-glucopyranose and phosphate from beta-D-glucopyranose 1-phosphate.<ref>PMID:24466148</ref> <ref>PMID:24828502</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacie]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fushinobu, S]]	+	[[Category: Fushinobu S]]
-	[[Category: Kitaoka, M]]	+	[[Category: Kitaoka M]]
-	[[Category: Nakai, H]]	+	[[Category: Nakai H]]
-	[[Category: Nihira, T]]	+	[[Category: Nihira T]]
-	[[Category: Touhara~~, K K]]~~	+	[[Category: Touhara KK]]
-	~~[[Category: Phosphorylase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:44, 3 April 2019

OCA — Wed, 03 Apr 2019 09:44:43 GMT

←Older revision		Revision as of 09:44, 3 April 2019
Line 1:		Line 1:

	==Crystal structure of 2-O-alpha-glucosylglycerol phosphorylase in complex with glucose==		==Crystal structure of 2-O-alpha-glucosylglycerol phosphorylase in complex with glucose==
-	<StructureSection load='4ktp' size='340' side='right' caption='[[4ktp]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='4ktp' size='340' side='right'caption='[[4ktp]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ktp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KTP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ktp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacie Bacie]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KTP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ktr\|4ktr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ktr\|4ktr]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Bsel_2816 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=439292 BACIE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [http://pdbe.org/4ktp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [http://www.ebi.ac.uk/pdbsum/4ktp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ktp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [http://pdbe.org/4ktp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [http://www.ebi.ac.uk/pdbsum/4ktp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ktp ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacie]]
		+	[[Category: Large Structures]]
	[[Category: Fushinobu, S]]		[[Category: Fushinobu, S]]
	[[Category: Kitaoka, M]]		[[Category: Kitaoka, M]]

OCA at 06:26, 5 August 2016

OCA — Fri, 05 Aug 2016 06:26:39 GMT

←Older revision		Revision as of 06:26, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of 2-O-alpha-glucosylglycerol phosphorylase in complex with glucose==		==Crystal structure of 2-O-alpha-glucosylglycerol phosphorylase in complex with glucose==
	<StructureSection load='4ktp' size='340' side='right' caption='[[4ktp]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4ktp' size='340' side='right' caption='[[4ktp]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ktr\|4ktr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ktr\|4ktr]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [http://www.ebi.ac.uk/pdbsum/4ktp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [http://pdbe.org/4ktp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [http://www.ebi.ac.uk/pdbsum/4ktp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ktp ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ktp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:22, 24 December 2014

OCA — Wed, 24 Dec 2014 11:22:41 GMT

←Older revision		Revision as of 11:22, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ktp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KTP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ktp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KTP FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ktr\|4ktr]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ktr\|4ktr]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [http://www.ebi.ac.uk/pdbsum/4ktp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [http://www.ebi.ac.uk/pdbsum/4ktp PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Structural basis for reversible phosphorolysis and hydrolysis reactions of 2-O-alpha-Glucosylglycerol phosphorylase.,Touhara KK, Nihira T, Kitaoka M, Nakai H, Fushinobu S J Biol Chem. 2014 May 14. pii: jbc.M114.573212. PMID:24828502<ref>PMID:24828502</ref>		Structural basis for reversible phosphorolysis and hydrolysis reactions of 2-O-alpha-Glucosylglycerol phosphorylase.,Touhara KK, Nihira T, Kitaoka M, Nakai H, Fushinobu S J Biol Chem. 2014 May 14. pii: jbc.M114.573212. PMID:24828502<ref>PMID:24828502</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Fushinobu, S.]]	+	[[Category: Fushinobu, S]]
-	[[Category: Kitaoka, M.]]	+	[[Category: Kitaoka, M]]
-	[[Category: Nakai, H.]]	+	[[Category: Nakai, H]]
-	[[Category: Nihira, T.]]	+	[[Category: Nihira, T]]
-	[[Category: Touhara, K K.]]	+	[[Category: Touhara, K K]]
	[[Category: Phosphorylase]]		[[Category: Phosphorylase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 07:13, 28 May 2014

OCA — Wed, 28 May 2014 07:13:53 GMT

←Older revision		Revision as of 07:13, 28 May 2014
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	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [http://www.ebi.ac.uk/pdbsum/4ktp PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [http://www.ebi.ac.uk/pdbsum/4ktp PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	2-O-alpha-Glucosylglycerol phosphorylase (GGP) from Bacillus selenitireducens catalyzes both the reversible phosphorolysis of 2-O-alpha-glucosylglycerol (GG) and the hydrolysis of beta-D-glucose 1-phosphate (betaGlc1P). GGP belongs to the glycoside hydrolase (GH) family 65 and can efficiently and specifically produce GG. However, its structural basis has remained unclear. In this study, the crystal structures of GGP complexed with glucose and with the glucose analogue isofagomine and glycerol were determined. Subsite -1 of GGP is similar to those of other GH65 enzymes, maltose phosphorylase and kojibiose phosphorylase, whereas subsite +1 is largely different and is well designed for GG recognition. An automated docking analysis was performed to complement these crystal structures, betaGlc1P being docked at an appropriate position. To investigate the importance of residues at subsite +1 in the bifunctionality of GGP, we constructed mutants at these residues. Y327F and K587A did not show detectable activities for either reverse phosphorolysis or betaGlc1P hydrolysis. Y572F also showed significantly reduced activities for both of these reactions. In contrast, W381F showed significantly reduced reverse phosphorolytic activity but retained betaGlc1P hydrolysis. The mode of substrate recognition and the reaction mechanisms of GGP were proposed based on these analyses. Specifically, an extensive hydrogen bond network formed by Tyr-327, Tyr-572, Lys-587, and water molecules contributes to fixing the acceptor molecule in both reverse phosphorolysis (glycerol) and betaGlc1P hydrolysis (water) for a glycosyl transfer reaction. This study will contribute to the development of a large-scale production system of GG by facilitating the rational engineering of GGP.
		+
		+	Structural basis for reversible phosphorolysis and hydrolysis reactions of 2-O-alpha-Glucosylglycerol phosphorylase.,Touhara KK, Nihira T, Kitaoka M, Nakai H, Fushinobu S J Biol Chem. 2014 May 14. pii: jbc.M114.573212. PMID:24828502<ref>PMID:24828502</ref>
		+
		+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:19, 21 May 2014

OCA — Wed, 21 May 2014 09:19:52 GMT

←Older revision		Revision as of 09:19, 21 May 2014
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-	'''~~Unreleased structure~~'''	+	==Crystal structure of 2-O-alpha-glucosylglycerol phosphorylase in complex with glucose==
-		+	<StructureSection load='4ktp' size='340' side='right' caption='[[4ktp]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~The entry~~ 4ktp is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4ktp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KTP FirstGlance]. <br>
-	~~Authors~~: ~~Touhara~~, K.K., ~~Nihira~~, T., Kitaoka, M., Nakai, H., ~~Fushinobu~~, S.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ktr\|4ktr]]</td></tr>
-	~~Description~~: ~~Crystal structure of a bacterial phosphorylase in complex with glucose~~	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ktp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ktp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ktp RCSB], [http://www.ebi.ac.uk/pdbsum/4ktp PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fushinobu, S.]]
		+	[[Category: Kitaoka, M.]]
		+	[[Category: Nakai, H.]]
		+	[[Category: Nihira, T.]]
		+	[[Category: Touhara, K K.]]
		+	[[Category: Phosphorylase]]
		+	[[Category: Transferase]]

OCA: Protected "4ktp" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2013 12:01:35 GMT

Protected "4ktp" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:01, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4ktp is ON HOLD until Paper Publication Authors: Touhara, K.K., Nihira, T., Kitaoka, M., Nakai, H., Fushinobu, S. Description: Crystal structure of...

OCA — Wed, 19 Jun 2013 12:01:34 GMT

New page: '''Unreleased structure''' The entry 4ktp is ON HOLD until Paper Publication Authors: Touhara, K.K., Nihira, T., Kitaoka, M., Nakai, H., Fushinobu, S. Description: Crystal structure of...

New page

'''Unreleased structure'''

The entry 4ktp is ON HOLD until Paper Publication

Authors: Touhara, K.K., Nihira, T., Kitaoka, M., Nakai, H., Fushinobu, S.

Description: Crystal structure of a bacterial phosphorylase in complex with glucose