4kvc - Revision history

OCA at 09:16, 7 December 2022

OCA — Wed, 07 Dec 2022 09:16:20 GMT

←Older revision		Revision as of 09:16, 7 December 2022
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	==2H2 Fab fragment of immature Dengue virus==		==2H2 Fab fragment of immature Dengue virus==
-	<StructureSection load='4kvc' size='340' side='right' caption='[[4kvc]], [[Resolution\|resolution]] 2.31Å' scene=''>	+	<StructureSection load='4kvc' size='340' side='right'caption='[[4kvc]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kvc]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KVC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4KVC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kvc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KVC FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Igh-1a, Igh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Gm16939 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kvc OCA], [https://pdbe.org/4kvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kvc RCSB], [https://www.ebi.ac.uk/pdbsum/4kvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kvc ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4kvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kvc OCA], [~~http~~://pdbe.org/4kvc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4kvc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4kvc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4kvc ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GCAA_MOUSE GCAA_MOUSE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Rossmann, M G~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Wang, Z~~]]	+	[[Category: Rossmann MG]]
-	[[Category: ~~Fab fragment]]~~	+	[[Category: Wang Z]]
-	~~[[Category: Immature dengue]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Pr peptide~~]]	+

OCA at 06:30, 5 August 2016

OCA — Fri, 05 Aug 2016 06:30:40 GMT

←Older revision		Revision as of 06:30, 5 August 2016
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		+
	==2H2 Fab fragment of immature Dengue virus==		==2H2 Fab fragment of immature Dengue virus==
	<StructureSection load='4kvc' size='340' side='right' caption='[[4kvc]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='4kvc' size='340' side='right' caption='[[4kvc]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kvc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KVC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KVC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kvc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KVC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KVC FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Igh-1a, Igh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~]), Gm16939 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Igh-1a, Igh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Gm16939 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kvc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kvc RCSB], [http://www.ebi.ac.uk/pdbsum/4kvc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kvc OCA], [http://pdbe.org/4kvc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kvc RCSB], [http://www.ebi.ac.uk/pdbsum/4kvc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kvc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4kvc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Rossmann, M G]]		[[Category: Rossmann, M G]]
	[[Category: Wang, Z]]		[[Category: Wang, Z]]

OCA at 15:04, 21 December 2014

OCA — Sun, 21 Dec 2014 15:04:05 GMT

←Older revision		Revision as of 15:04, 21 December 2014
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-	~~{{STRUCTURE_4kvc~~\| ~~PDB~~=4kvc \| ~~SCENE~~= }}	+	==2H2 Fab fragment of immature Dengue virus==
-	===2H2 Fab ~~fragment~~ of immature ~~Dengue~~ virus~~===~~	+	<StructureSection load='4kvc' size='340' side='right' caption='[[4kvc]], [[Resolution\|resolution]] 2.31Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23740974}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4kvc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KVC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KVC FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Igh-1a, Igh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), Gm16939 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kvc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kvc RCSB], [http://www.ebi.ac.uk/pdbsum/4kvc PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The 2H2 monoclonal antibody recognizes the precursor peptide on the immature dengue virus and might, therefore, be a useful tool for investigating the conformational change that occurs when the immature virus enters an acidic environment. During dengue virus maturation, the spiky, immature, non-infectious virions change their structure to smooth-surfaced particles in the slightly acid environment of the trans-Golgi network, thereby allowing cellular furin to cleave the precursor-membrane proteins. The dengue virions become fully infectious when they release the cleaved precursor peptide on reaching the neutral pH environment of the extracellular space. Here we report on the cryo-electron microscopy structures of the immature virus complexed with the 2H2 antigen binding fragments (Fab) at different concentrations and varied pH conditions. At neutral pH and high concentration of the Fab molecules, three Fab molecules bind to three precursor-membrane proteins on each spike of the immature virus. However, at a low concentration of the Fab molecules and at pH 7.0, only two Fab molecules bind to each spike. Changing to slightly acidic pH caused no detectable change of structure for the high Fab concentration sample, but caused severe structural damage to the low concentration sample. Therefore, the 2H2 Fab inhibits the maturation process of immature dengue virus when the Fab molecules are at high concentration, because the three Fab molecules on each spike hold the precursor-membrane molecules together, thereby inhibiting the normal conformational change that occurs during maturation.

-	~~==About this Structure==~~	+	Obstruction of Dengue Virus Maturation by Fab Fragments of the 2H2 Antibody.,Wang Z, Li L, Pennington JG, Sheng J, Yap ML, Plevka P, Meng G, Sun L, Jiang W, Rossmann MG J Virol. 2013 Jun 5. PMID:23740974<ref>PMID:23740974</ref>
-	~~[[4kvc]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4KVC OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023740974</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Rossmann, M G.]]	+	[[Category: Rossmann, M G]]
-	[[Category: Wang, Z.]]	+	[[Category: Wang, Z]]
	[[Category: Fab fragment]]		[[Category: Fab fragment]]
	[[Category: Immature dengue]]		[[Category: Immature dengue]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Pr peptide]]		[[Category: Pr peptide]]

OCA at 12:59, 24 July 2013

OCA — Wed, 24 Jul 2013 12:59:07 GMT

←Older revision		Revision as of 12:59, 24 July 2013
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4kvc\| PDB=4kvc \| SCENE= }}
		+	===2H2 Fab fragment of immature Dengue virus===
		+	{{ABSTRACT_PUBMED_23740974}}

-	~~The entry~~ 4kvc is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4kvc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KVC OCA].

-	~~Authors~~: ~~Wang, Z.,~~ Rossmann, M.G.	+	==Reference==
-		+	<ref group="xtra">PMID:023740974</ref><references group="xtra"/><references/>
-	~~Description~~: ~~2H2~~ Fab fragment ~~of immature Dengue virus~~	+	[[Category: Mus musculus]]
		+	[[Category: Rossmann, M G.]]
		+	[[Category: Wang, Z.]]
		+	[[Category: Fab fragment]]
		+	[[Category: Immature dengue]]
		+	[[Category: Immune system]]
		+	[[Category: Pr peptide]]

OCA at 15:02, 10 July 2013

OCA — Wed, 10 Jul 2013 15:02:57 GMT

←Older revision		Revision as of 15:02, 10 July 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4kvc is ON HOLD	+	The entry 4kvc is ON HOLD until Paper Publication

	Authors: Wang, Z., Rossmann, M.G.		Authors: Wang, Z., Rossmann, M.G.

	Description: 2H2 Fab fragment of immature Dengue virus		Description: 2H2 Fab fragment of immature Dengue virus

OCA: Protected "4kvc" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2013 12:05:37 GMT

Protected "4kvc" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:05, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4kvc is ON HOLD Authors: Wang, Z., Rossmann, M.G. Description: 2H2 Fab fragment of immature Dengue virus

OCA — Wed, 19 Jun 2013 12:05:36 GMT

New page: '''Unreleased structure''' The entry 4kvc is ON HOLD Authors: Wang, Z., Rossmann, M.G. Description: 2H2 Fab fragment of immature Dengue virus

New page

'''Unreleased structure'''

The entry 4kvc is ON HOLD

Authors: Wang, Z., Rossmann, M.G.

Description: 2H2 Fab fragment of immature Dengue virus