4kxj - Revision history

OCA at 08:54, 20 March 2024

OCA — Wed, 20 Mar 2024 08:54:55 GMT

←Older revision		Revision as of 08:54, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4kxj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_coronavirus_OC43 Human coronavirus OC43]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KXJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4kxj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_coronavirus_OC43 Human coronavirus OC43]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KXJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P34:N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE'>P34</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P34:N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE'>P34</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxj OCA], [https://pdbe.org/4kxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kxj RCSB], [https://www.ebi.ac.uk/pdbsum/4kxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxj OCA], [https://pdbe.org/4kxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kxj RCSB], [https://www.ebi.ac.uk/pdbsum/4kxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/NCAP_CVHOC NCAP_CVHOC] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.[HAMAP-Rule:MF_04096]		[https://www.uniprot.org/uniprot/NCAP_CVHOC NCAP_CVHOC] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.[HAMAP-Rule:MF_04096]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Coronaviruses (CoVs) cause numerous diseases, including Middle East respiratory syndrome and severe acute respiratory syndrome, generating significant health-related and economic consequences. CoVs encode the nucleocapsid (N) protein, a major structural protein that plays multiple roles in the virus replication cycle and forms a ribonucleoprotein complex with the viral RNA through the N protein's N-terminal domain (N-NTD). Using human CoV-OC43 (HCoV-OC43) as a model for CoV, we present the 3D structure of HCoV-OC43 N-NTD complexed with ribonucleoside 5'-monophosphates to identify a distinct ribonucleotide-binding pocket. By targeting this pocket, we identified and developed a new coronavirus N protein inhibitor, N-(6-oxo-5,6-dihydrophenanthridin-2-yl)(N,N-dimethylamino)acetamide hydrochloride (PJ34), using virtual screening; this inhibitor reduced the N protein's RNA-binding affinity and hindered viral replication. We also determined the crystal structure of the N-NTD-PJ34 complex. On the basis of these findings, we propose guidelines for developing new N protein-based antiviral agents that target CoVs.
-
-	Structural basis for the identification of the N-terminal domain of coronavirus nucleocapsid protein as an antiviral target.,Lin SY, Liu CL, Chang YM, Zhao J, Perlman S, Hou MH J Med Chem. 2014 Mar 27;57(6):2247-57. doi: 10.1021/jm500089r. Epub 2014 Mar 12. PMID:24564608<ref>PMID:24564608</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4kxj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]		*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:20, 7 December 2022

OCA — Wed, 07 Dec 2022 09:20:11 GMT

←Older revision		Revision as of 09:20, 7 December 2022
Line 3:		Line 3:
	<StructureSection load='4kxj' size='340' side='right'caption='[[4kxj]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='4kxj' size='340' side='right'caption='[[4kxj]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kxj]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cvhoc Cvhoc~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4KXJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kxj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_coronavirus_OC43 Human coronavirus OC43]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KXJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P34:N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE'>P34</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P34:N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE'>P34</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31631 CVHOC])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxj OCA], [https://pdbe.org/4kxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kxj RCSB], [https://www.ebi.ac.uk/pdbsum/4kxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxj ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4kxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxj OCA], [~~http~~://pdbe.org/4kxj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4kxj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4kxj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxj ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~Q6SA23_CVHOC Q6SA23_CVHOC~~]~~] Major structural component of virions that associates with genomic~~ RNA ~~to form~~ a ~~long, flexible,~~ helical ~~nucleocapsid~~ (~~By similarity~~).[~~PIRNR~~:~~PIRNR003888~~]	+	[https://www.uniprot.org/uniprot/NCAP_CVHOC NCAP_CVHOC] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.[HAMAP-Rule:MF_04096]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
-	*[[Nucleoprotein\|Nucleoprotein]]	+	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cvhoc~~]]	+	[[Category: Human coronavirus OC43]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hou~~, M H~~]]	+	[[Category: Hou MH]]
-	[[Category: Lin~~, S Y]]~~	+	[[Category: Lin SY]]
-	~~[[Category: Hcov-oc43 n-ntd]]~~	+
-	~~[[Category: N-protein ligand]]~~	+
-	~~[[Category: Rna binding protein~~]]	+

OCA at 09:41, 3 April 2019

OCA — Wed, 03 Apr 2019 09:41:05 GMT

←Older revision		Revision as of 09:41, 3 April 2019
Line 1:		Line 1:

	==Crystal structure of HCoV-OC43 N-NTD complexed with PJ34==		==Crystal structure of HCoV-OC43 N-NTD complexed with PJ34==
-	<StructureSection load='4kxj' size='340' side='right' caption='[[4kxj]], [[Resolution\|resolution]] 2.65Å' scene=''>	+	<StructureSection load='4kxj' size='340' side='right'caption='[[4kxj]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kxj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KXJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kxj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvhoc Cvhoc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KXJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P34:N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE'>P34</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P34:N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE'>P34</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31631 CVHOC])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxj OCA], [http://pdbe.org/4kxj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kxj RCSB], [http://www.ebi.ac.uk/pdbsum/4kxj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxj OCA], [http://pdbe.org/4kxj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kxj RCSB], [http://www.ebi.ac.uk/pdbsum/4kxj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxj ProSAT]</span></td></tr>
	</table>		</table>
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cvhoc]]
		+	[[Category: Large Structures]]
	[[Category: Hou, M H]]		[[Category: Hou, M H]]
	[[Category: Lin, S Y]]		[[Category: Lin, S Y]]

OCA at 23:24, 5 August 2016

OCA — Fri, 05 Aug 2016 23:24:15 GMT

←Older revision		Revision as of 23:24, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of HCoV-OC43 N-NTD complexed with PJ34==		==Crystal structure of HCoV-OC43 N-NTD complexed with PJ34==
	<StructureSection load='4kxj' size='340' side='right' caption='[[4kxj]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='4kxj' size='340' side='right' caption='[[4kxj]], [[Resolution\|resolution]] 2.65Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4kxj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KXJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4kxj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KXJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P34:N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE'>P34</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P34:N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE'>P34</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kxj RCSB], [http://www.ebi.ac.uk/pdbsum/4kxj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxj OCA], [http://pdbe.org/4kxj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kxj RCSB], [http://www.ebi.ac.uk/pdbsum/4kxj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4kxj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nucleoprotein\|Nucleoprotein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:45, 25 December 2014

OCA — Thu, 25 Dec 2014 06:45:56 GMT

←Older revision		Revision as of 06:45, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4kxj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KXJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4kxj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KXJ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P34:N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE'>P34</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P34:N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE'>P34</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kxj RCSB], [http://www.ebi.ac.uk/pdbsum/4kxj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kxj RCSB], [http://www.ebi.ac.uk/pdbsum/4kxj PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q6SA23_CVHOC Q6SA23_CVHOC]] Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid (By similarity).[PIRNR:PIRNR003888]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 12:		Line 14:
	Structural basis for the identification of the N-terminal domain of coronavirus nucleocapsid protein as an antiviral target.,Lin SY, Liu CL, Chang YM, Zhao J, Perlman S, Hou MH J Med Chem. 2014 Mar 27;57(6):2247-57. doi: 10.1021/jm500089r. Epub 2014 Mar 12. PMID:24564608<ref>PMID:24564608</ref>		Structural basis for the identification of the N-terminal domain of coronavirus nucleocapsid protein as an antiviral target.,Lin SY, Liu CL, Chang YM, Zhao J, Perlman S, Hou MH J Med Chem. 2014 Mar 27;57(6):2247-57. doi: 10.1021/jm500089r. Epub 2014 Mar 12. PMID:24564608<ref>PMID:24564608</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 18:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Hou, M H.]]	+	[[Category: Hou, M H]]
-	[[Category: Lin, S Y.]]	+	[[Category: Lin, S Y]]
	[[Category: Hcov-oc43 n-ntd]]		[[Category: Hcov-oc43 n-ntd]]
	[[Category: N-protein ligand]]		[[Category: N-protein ligand]]
	[[Category: Rna binding protein]]		[[Category: Rna binding protein]]

OCA at 09:42, 28 May 2014

OCA — Wed, 28 May 2014 09:42:58 GMT

←Older revision		Revision as of 09:42, 28 May 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of HCoV-OC43 N-NTD complexed with PJ34==
		+	<StructureSection load='4kxj' size='340' side='right' caption='[[4kxj]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4kxj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KXJ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P34:N~2~,N~2~-DIMETHYL-N~1~-(6-OXO-5,6-DIHYDROPHENANTHRIDIN-2-YL)GLYCINAMIDE'>P34</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kxj RCSB], [http://www.ebi.ac.uk/pdbsum/4kxj PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Coronaviruses (CoVs) cause numerous diseases, including Middle East respiratory syndrome and severe acute respiratory syndrome, generating significant health-related and economic consequences. CoVs encode the nucleocapsid (N) protein, a major structural protein that plays multiple roles in the virus replication cycle and forms a ribonucleoprotein complex with the viral RNA through the N protein's N-terminal domain (N-NTD). Using human CoV-OC43 (HCoV-OC43) as a model for CoV, we present the 3D structure of HCoV-OC43 N-NTD complexed with ribonucleoside 5'-monophosphates to identify a distinct ribonucleotide-binding pocket. By targeting this pocket, we identified and developed a new coronavirus N protein inhibitor, N-(6-oxo-5,6-dihydrophenanthridin-2-yl)(N,N-dimethylamino)acetamide hydrochloride (PJ34), using virtual screening; this inhibitor reduced the N protein's RNA-binding affinity and hindered viral replication. We also determined the crystal structure of the N-NTD-PJ34 complex. On the basis of these findings, we propose guidelines for developing new N protein-based antiviral agents that target CoVs.

-	~~The entry 4kxj is ON HOLD until Paper Publication~~	+	Structural basis for the identification of the N-terminal domain of coronavirus nucleocapsid protein as an antiviral target.,Lin SY, Liu CL, Chang YM, Zhao J, Perlman S, Hou MH J Med Chem. 2014 Mar 27;57(6):2247-57. doi: 10.1021/jm500089r. Epub 2014 Mar 12. PMID:24564608<ref>PMID:24564608</ref>

-	~~Authors: Lin~~, S.Y., Hou, M.H.	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of HCoV~~-~~OC43~~ N-~~NTD complexed with PJ34~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hou, M H.]]
		+	[[Category: Lin, S Y.]]
		+	[[Category: Hcov-oc43 n-ntd]]
		+	[[Category: N-protein ligand]]
		+	[[Category: Rna binding protein]]

OCA at 08:56, 8 January 2014

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	Authors: Lin, S.Y., Hou, M.H.		Authors: Lin, S.Y., Hou, M.H.

	Description: Crystal structure of HCoV-OC43 N-NTD complexed with PJ34		Description: Crystal structure of HCoV-OC43 N-NTD complexed with PJ34

OCA: Protected "4kxj" [edit=sysop:move=sysop]

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←Older revision

Revision as of 12:11, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4kxj is ON HOLD Authors: Lin, S.Y., Hou, M.H. Description: Crystal structure of HCoV-OC43 N-NTD complexed with PJ34

OCA — Wed, 19 Jun 2013 12:11:22 GMT

New page: '''Unreleased structure''' The entry 4kxj is ON HOLD Authors: Lin, S.Y., Hou, M.H. Description: Crystal structure of HCoV-OC43 N-NTD complexed with PJ34

New page

'''Unreleased structure'''

The entry 4kxj is ON HOLD

Authors: Lin, S.Y., Hou, M.H.

Description: Crystal structure of HCoV-OC43 N-NTD complexed with PJ34