4kxo - Revision history

OCA at 16:06, 20 September 2023

OCA — Wed, 20 Sep 2023 16:06:48 GMT

←Older revision		Revision as of 16:06, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4kxo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KXO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4kxo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KXO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxo OCA], [https://pdbe.org/4kxo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kxo RCSB], [https://www.ebi.ac.uk/pdbsum/4kxo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxo ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxo OCA], [https://pdbe.org/4kxo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kxo RCSB], [https://www.ebi.ac.uk/pdbsum/4kxo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 09:20, 7 December 2022

OCA — Wed, 07 Dec 2022 09:20:20 GMT

←Older revision		Revision as of 09:20, 7 December 2022
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	==Crystal Structure of BBBB at pH 10.0 with MPD as the cryoprotectant==		==Crystal Structure of BBBB at pH 10.0 with MPD as the cryoprotectant==
-	<StructureSection load='4kxo' size='340' side='right' caption='[[4kxo]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4kxo' size='340' side='right'caption='[[4kxo]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kxo]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4KXO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kxo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KXO FirstGlance]. <br>
-	</td></tr>~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxo OCA], [https://pdbe.org/4kxo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kxo RCSB], [https://www.ebi.ac.uk/pdbsum/4kxo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxo ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4kxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxo OCA], [~~http~~://pdbe.org/4kxo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4kxo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4kxo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxo ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BGAT_HUMAN BGAT_HUMAN] This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4kxo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4kxo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Fucosylgalactoside 3-alpha-galactosyltransferase~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Human~~]]	+	[[Category: Large Structures]]
-	[[Category: Alfaro~~, J A~~]]	+	[[Category: Alfaro JA]]
-	[[Category: Blackler~~, R J~~]]	+	[[Category: Blackler RJ]]
-	[[Category: Borisova~~, S N~~]]	+	[[Category: Borisova SN]]
-	[[Category: Evans~~, S V~~]]	+	[[Category: Evans SV]]
-	[[Category: Johal~~, A R~~]]	+	[[Category: Johal AR]]
-	[[Category: Schuman, B]]	+	[[Category: Schuman B]]
-	~~[[Category: Abo rossmann fold]]~~	+
-	~~[[Category: Blood group antigen]]~~	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Manganese]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Retaining glycosyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 15:24, 11 August 2016

OCA — Thu, 11 Aug 2016 15:24:04 GMT

←Older revision		Revision as of 15:24, 11 August 2016
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		+
	==Crystal Structure of BBBB at pH 10.0 with MPD as the cryoprotectant==		==Crystal Structure of BBBB at pH 10.0 with MPD as the cryoprotectant==
	<StructureSection load='4kxo' size='340' side='right' caption='[[4kxo]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4kxo' size='340' side='right' caption='[[4kxo]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kxo RCSB], [http://www.ebi.ac.uk/pdbsum/4kxo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxo OCA], [http://pdbe.org/4kxo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kxo RCSB], [http://www.ebi.ac.uk/pdbsum/4kxo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kxo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4kxo" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:58, 4 January 2015

OCA — Sun, 04 Jan 2015 13:58:47 GMT

←Older revision		Revision as of 13:58, 4 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4kxo\| PDB=4kxo \| SCENE= }}~~	+	==Crystal Structure of BBBB at pH 10.0 with MPD as the cryoprotectant==
-	===Crystal Structure of BBBB at pH 10.0 with MPD as the cryoprotectant===	+	<StructureSection load='4kxo' size='340' side='right' caption='[[4kxo]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24265507}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4kxo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KXO FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ABO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fucosylgalactoside_3-alpha-galactosyltransferase Fucosylgalactoside 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.37 2.4.1.37] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kxo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kxo RCSB], [http://www.ebi.ac.uk/pdbsum/4kxo PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The homologous human ABO(H) A and B blood group glycosyltransferases GTA and GTB have two mobile polypeptide loops surrounding their active sites that serve to allow substrate access and product egress and to recognize and sequester substrates for catalysis. Previous studies have established that these enzymes can move from the 'open' state to the 'semi-closed' then 'closed' states in response to addition of substrate. The contribution of electrostatic interactions to these conformational changes has now been demonstrated by the determination at various pH of the structures of GTA, GTB, and the chimeric enzyme ABBA. Near neutral pH, GTA displays the closed state in which both mobile loops order around the active site, whereas ABBA and GTB display the open state. At low pH the apparent protonation of the DXD motif in GTA leads to expulsion of the donor analog to yield the open state, while at high pH both ABBA and GTB form the semi-closed state in which the first mobile loop becomes an ordered alpha-helix. Step-wise deprotonation of GTB in increments of 0.5 between pH 6.5 and 10.0 shows that helix ordering is gradual, which indicates that the formation of the semi-closed state is dependent on electrostatic forces consistent with the binding of substrate. Spectropolarimetric studies of the corresponding stand-alone peptide in solution reveal no tendency towards helix formation from pH 7.0 to 10.0, which shows that pH-dependent stability is a product of the larger protein environment and underlines the importance of substrate in active site ordering.

-	~~==About this Structure==~~	+	pH-induced conformational changes in human ABO(H) blood group glycosyltransferases confirm the importance of electrostatic interactions in formation of the semi-closed state.,Johal AR, Blackler RJ, Alfaro JA, Schuman B, Borisova S, Evans SV Glycobiology. 2013 Nov 20. PMID:24265507<ref>PMID:24265507</ref>
-	~~[[4kxo]] is a 1 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4KXO OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024265507</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]		[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]
-	[[Category: Alfaro, J A.]]	+	[[Category: Human]]
-	[[Category: Blackler, R J.]]	+	[[Category: Alfaro, J A]]
-	[[Category: Borisova, S N.]]	+	[[Category: Blackler, R J]]
-	[[Category: Evans, S V.]]	+	[[Category: Borisova, S N]]
-	[[Category: Johal, A R.]]	+	[[Category: Evans, S V]]
-	[[Category: Schuman, B.]]	+	[[Category: Johal, A R]]
		+	[[Category: Schuman, B]]
	[[Category: Abo rossmann fold]]		[[Category: Abo rossmann fold]]
	[[Category: Blood group antigen]]		[[Category: Blood group antigen]]

OCA at 06:31, 9 April 2014

OCA — Wed, 09 Apr 2014 06:31:47 GMT

←Older revision		Revision as of 06:31, 9 April 2014
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4kxo\| PDB=4kxo \| SCENE= }}
		+	===Crystal Structure of BBBB at pH 10.0 with MPD as the cryoprotectant===
		+	{{ABSTRACT_PUBMED_24265507}}

-	~~The entry~~ 4kxo is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4kxo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KXO OCA].

-	~~Authors~~: ~~Johal~~, A.~~R.,~~ Blackler, R.J.~~, Alfaro, J.A., Schuman, B.,~~ Borisova, S.N., Evans, S.V.	+	==Reference==
-		+	<ref group="xtra">PMID:024265507</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal Structure of BBBB at pH 10~~.~~0 with MPD as the cryoprotectant~~	+	[[Category: Fucosylgalactoside 3-alpha-galactosyltransferase]]
		+	[[Category: Alfaro, J A.]]
		+	[[Category: Blackler, R J.]]
		+	[[Category: Borisova, S N.]]
		+	[[Category: Evans, S V.]]
		+	[[Category: Johal, A R.]]
		+	[[Category: Schuman, B.]]
		+	[[Category: Abo rossmann fold]]
		+	[[Category: Blood group antigen]]
		+	[[Category: Glycoprotein]]
		+	[[Category: Manganese]]
		+	[[Category: Metal-binding]]
		+	[[Category: Retaining glycosyltransferase]]
		+	[[Category: Transferase]]

OCA: Protected "4kxo" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2013 12:11:45 GMT

Protected "4kxo" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:11, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4kxo is ON HOLD until Paper Publication Authors: Johal, A.R., Blackler, R.J., Alfaro, J.A., Schuman, B., Borisova, S.N., Evans, S.V. Description: C...

OCA — Wed, 19 Jun 2013 12:11:44 GMT

New page: '''Unreleased structure''' The entry 4kxo is ON HOLD until Paper Publication Authors: Johal, A.R., Blackler, R.J., Alfaro, J.A., Schuman, B., Borisova, S.N., Evans, S.V. Description: C...

New page

'''Unreleased structure'''

The entry 4kxo is ON HOLD until Paper Publication

Authors: Johal, A.R., Blackler, R.J., Alfaro, J.A., Schuman, B., Borisova, S.N., Evans, S.V.

Description: Crystal Structure of BBBB at pH 10.0 with MPD as the cryoprotectant