4kzt - Revision history

OCA at 16:08, 20 September 2023

OCA — Wed, 20 Sep 2023 16:08:02 GMT

←Older revision		Revision as of 16:08, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4kzt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Maricaulis_maris_MCS10 Maricaulis maris MCS10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KZT FirstGlance]. <br>		<table><tr><td colspan='2'>[[4kzt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Maricaulis_maris_MCS10 Maricaulis maris MCS10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KZT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kzt OCA], [https://pdbe.org/4kzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kzt RCSB], [https://www.ebi.ac.uk/pdbsum/4kzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kzt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kzt OCA], [https://pdbe.org/4kzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kzt RCSB], [https://www.ebi.ac.uk/pdbsum/4kzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kzt ProSAT]</span></td></tr>
	</table>		</table>

OCA at 09:23, 7 December 2022

OCA — Wed, 07 Dec 2022 09:23:03 GMT

←Older revision		Revision as of 09:23, 7 December 2022
Line 1:		Line 1:

	==Structure mmNAGS bound with L-arginine==		==Structure mmNAGS bound with L-arginine==
-	<StructureSection load='4kzt' size='340' side='right' caption='[[4kzt]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='4kzt' size='340' side='right'caption='[[4kzt]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kzt]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Marmm Marmm~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KZT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4KZT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kzt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Maricaulis_maris_MCS10 Maricaulis maris MCS10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KZT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s6h\|3s6h]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kzt OCA], [https://pdbe.org/4kzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kzt RCSB], [https://www.ebi.ac.uk/pdbsum/4kzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kzt ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=394221 MARMM])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4kzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kzt OCA], [~~http~~://pdbe.org/4kzt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4kzt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4kzt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4kzt ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q0ASS9_MARMM Q0ASS9_MARMM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Marmm~~]]	+	[[Category: Large Structures]]
-	[[Category: Allewell~~, N M~~]]	+	[[Category: Maricaulis maris MCS10]]
-	[[Category: Jin, Z]]	+	[[Category: Allewell NM]]
-	[[Category: Shi, D]]	+	[[Category: Jin Z]]
-	[[Category: Tuchman, M]]	+	[[Category: Shi D]]
-	[[Category: Zhao, G]]	+	[[Category: Tuchman M]]
-	~~[[Category: Kinase]]~~	+	[[Category: Zhao G]]
-	~~[[Category: Synthetase]]~~	+
-	~~[[Category: Transferase-transferase inhibitor complex~~]]	+

OCA at 12:13, 11 August 2016

OCA — Thu, 11 Aug 2016 12:13:36 GMT

←Older revision		Revision as of 12:13, 11 August 2016
Line 1:		Line 1:
		+
	==Structure mmNAGS bound with L-arginine==		==Structure mmNAGS bound with L-arginine==
	<StructureSection load='4kzt' size='340' side='right' caption='[[4kzt]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='4kzt' size='340' side='right' caption='[[4kzt]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s6h\|3s6h]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s6h\|3s6h]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=394221 MARMM])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=394221 MARMM])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kzt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kzt RCSB], [http://www.ebi.ac.uk/pdbsum/4kzt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kzt OCA], [http://pdbe.org/4kzt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kzt RCSB], [http://www.ebi.ac.uk/pdbsum/4kzt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kzt ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4kzt" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:22, 4 January 2015

OCA — Sun, 04 Jan 2015 14:22:22 GMT

←Older revision		Revision as of 14:22, 4 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4kzt\| PDB~~=4kzt \| ~~SCENE~~= }}	+	==Structure mmNAGS bound with L-arginine==
-	===~~Structure mmNAGS bound~~ with L-~~arginine~~===	+	<StructureSection load='4kzt' size='340' side='right' caption='[[4kzt]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23850694}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4kzt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Marmm Marmm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KZT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s6h\|3s6h]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">argA/B, Mmar10_0365 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=394221 MARMM])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kzt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kzt RCSB], [http://www.ebi.ac.uk/pdbsum/4kzt PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Maricaulis maris N-acetylglutamate synthase/kinase (mmNAGS/K) catalyzes the first two steps in L-arginine biosynthesis and has a high degree of sequence and structural homology to human N-acetylglutamate synthase, a regulator of the urea cycle. The synthase activity of both mmNAGS/K and human NAGS are regulated by L-arginine, although L-arginine is an allosteric inhibitor of mmNAGS/K, but an activator of human NAGS. To investigate the mechanism of allosteric inhibition of mmNAGS/K by L-arginine, we have determined the structure of the mmNAGS/K complexed with L-arginine at 2.8 A resolution. In contrast to the structure of mmNAGS/K in the absence of L-arginine where there are conformational differences between the four subunits in the asymmetric unit, all four subunits in the L-arginine liganded structure have very similar conformations. In this conformation, the AcCoA binding site in the N-acetyltransferase (NAT) domain is blocked by a loop from the amino acid kinase (AAK) domain, as a result of a domain rotation that occurs when L-arginine binds. This structural change provides an explanation for the allosteric inhibition of mmNAGS/K and related enzymes by L-arginine. The allosterically regulated mechanism for mmNAGS/K differs significantly from that for Neisseria gonorrhoeae NAGS (ngNAGS). To define the active site, several residues near the putative active site were mutated and their activities determined. These experiments identify roles for Lys356, Arg386, Asn391 and Tyr397 in the catalytic mechanism.

-	~~==About this~~ Structure==	+	Structure of N-acetyl-L-glutamate synthase/kinase from Maricaulis maris with the allosteric inhibitor L-arginine bound.,Zhao G, Haskins N, Jin Z, M Allewell N, Tuchman M, Shi D Biochem Biophys Res Commun. 2013 Aug 9;437(4):585-90. doi:, 10.1016/j.bbrc.2013.07.003. Epub 2013 Jul 10. PMID:23850694<ref>PMID:23850694</ref>
-	~~[[4kzt]] is a~~ 4 ~~chain structure~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.~~il/oca-bin/ocashort?id=4KZT OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023850694</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
-	[[Category: Allewell, N M.]]	+	== References ==
-	[[Category: Jin, Z.]]	+	<references/>
-	[[Category: Shi, D.]]	+	__TOC__
-	[[Category: Tuchman, M.]]	+	</StructureSection>
-	[[Category: Zhao, G.]]	+	[[Category: Marmm]]
		+	[[Category: Allewell, N M]]
		+	[[Category: Jin, Z]]
		+	[[Category: Shi, D]]
		+	[[Category: Tuchman, M]]
		+	[[Category: Zhao, G]]
	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Synthetase]]		[[Category: Synthetase]]
	[[Category: Transferase-transferase inhibitor complex]]		[[Category: Transferase-transferase inhibitor complex]]

OCA at 09:38, 16 April 2014

OCA — Wed, 16 Apr 2014 09:38:07 GMT

←Older revision		Revision as of 09:38, 16 April 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4kzt\| PDB=4kzt \| SCENE= }}
		+	===Structure mmNAGS bound with L-arginine===
		+	{{ABSTRACT_PUBMED_23850694}}

-	~~The entry~~ 4kzt is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4kzt]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KZT OCA].

-	~~Authors~~: ~~Zhao~~, G., Jin, Z., ~~Allewell, N~~.~~M.,~~ Tuchman, M., ~~Shi, D~~.	+	==Reference==
-		+	<ref group="xtra">PMID:023850694</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Structure mmNAGS bound with L~~-~~arginine~~	+	[[Category: Allewell, N M.]]
		+	[[Category: Jin, Z.]]
		+	[[Category: Shi, D.]]
		+	[[Category: Tuchman, M.]]
		+	[[Category: Zhao, G.]]
		+	[[Category: Kinase]]
		+	[[Category: Synthetase]]
		+	[[Category: Transferase-transferase inhibitor complex]]

OCA at 12:23, 24 July 2013

OCA — Wed, 24 Jul 2013 12:23:16 GMT

←Older revision		Revision as of 12:23, 24 July 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4kzt is ON HOLD	+	The entry 4kzt is ON HOLD until Paper Publication

-	Authors: Zhao, G, Jin, Z., Allewell, N.M., Tuchman, M., Shi, D.	+	Authors: Zhao, G., Jin, Z., Allewell, N.M., Tuchman, M., Shi, D.

	Description: Structure mmNAGS bound with L-arginine		Description: Structure mmNAGS bound with L-arginine

OCA: Protected "4kzt" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2013 12:17:22 GMT

Protected "4kzt" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:17, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4kzt is ON HOLD Authors: Zhao, G, Jin, Z., Allewell, N.M., Tuchman, M., Shi, D. Description: Structure mmNAGS bound with L-arginine

OCA — Wed, 19 Jun 2013 12:17:21 GMT

New page: '''Unreleased structure''' The entry 4kzt is ON HOLD Authors: Zhao, G, Jin, Z., Allewell, N.M., Tuchman, M., Shi, D. Description: Structure mmNAGS bound with L-arginine

New page

'''Unreleased structure'''

The entry 4kzt is ON HOLD

Authors: Zhao, G, Jin, Z., Allewell, N.M., Tuchman, M., Shi, D.

Description: Structure mmNAGS bound with L-arginine