4l6o - Revision history

OCA at 12:17, 1 March 2024

OCA — Fri, 01 Mar 2024 12:17:53 GMT

←Older revision		Revision as of 12:17, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4l6o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_SC5314 Candida albicans SC5314]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L6O FirstGlance]. <br>		<table><tr><td colspan='2'>[[4l6o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_SC5314 Candida albicans SC5314]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L6O FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.88Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6o OCA], [https://pdbe.org/4l6o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l6o RCSB], [https://www.ebi.ac.uk/pdbsum/4l6o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l6o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6o OCA], [https://pdbe.org/4l6o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l6o RCSB], [https://www.ebi.ac.uk/pdbsum/4l6o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l6o ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/METE_CANAL METE_CANAL] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]		[https://www.uniprot.org/uniprot/METE_CANAL METE_CANAL] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The cobalamin-independent methionine synthase from Candida albicans, known as Met6p, is a 90-kDa enzyme that consists of two (betaalpha)8 barrels. The active site is located between the two domains and has binding sites for a zinc ion and substrates l-homocysteine and 5-methyl-tetrahydrofolate-glutamate3. Met6p catalyzes transfer of the methyl group of 5-methyl-tetrahydrofolate-glutamate3 to the l-homocysteine thiolate to generate methionine. Met6p is essential for fungal growth, and we currently pursue it as an antifungal drug design target. Here we report the binding of l-homocysteine, methionine, and several folate analogs. We show that binding of l-homocysteine or methionine results in conformational rearrangements at the amino acid binding pocket, moving the catalytic zinc into position to activate the thiol group. We also map the folate binding pocket and identify specific binding residues, like Asn126, whose mutation eliminates catalytic activity. We also report the development of a robust fluorescence-based activity assay suitable for high-throughput screening. We use this assay and an X-ray structure to characterize methotrexate as a weak inhibitor of fungal Met6p.

-	Structural analysis of a fungal methionine synthase with substrates and inhibitors.,Ubhi D, Kago G, Monzingo AF, Robertus JD J Mol Biol. 2014 Apr 17;426(8):1839-47. doi: 10.1016/j.jmb.2014.02.006. Epub 2014, Feb 11. PMID:24524835<ref>PMID:24524835</ref>	+	==See Also==
-		+	*[[Methionine synthase 3D structures\|Methionine synthase 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 4l6o" style~~=~~"background-color:#fffaf0;"></div>~~	+
-	~~== References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:35, 7 December 2022

OCA — Wed, 07 Dec 2022 09:35:12 GMT

←Older revision		Revision as of 09:35, 7 December 2022
Line 1:		Line 1:

	==Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine==		==Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine==
-	<StructureSection load='4l6o' size='340' side='right' caption='[[4l6o]], [[Resolution\|resolution]] 1.88Å' scene=''>	+	<StructureSection load='4l6o' size='340' side='right'caption='[[4l6o]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4l6o]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Canal Canal~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4L6O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4l6o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_SC5314 Candida albicans SC5314]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L6O FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l5z\|4l5z]], [[4l61\|4l61]], [[4l64\|4l64]], [[4l65\|4l65]], [[4l6h\|4l6h]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6o OCA], [https://pdbe.org/4l6o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l6o RCSB], [https://www.ebi.ac.uk/pdbsum/4l6o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l6o ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CaO19.10083, CaO19.2551, MET6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237561 CANAL])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4l6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6o OCA], [~~http~~://pdbe.org/4l6o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4l6o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4l6o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4l6o ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/METE_CANAL METE_CANAL]] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]	+	[https://www.uniprot.org/uniprot/METE_CANAL METE_CANAL] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase~~]]	+	[[Category: Candida albicans SC5314]]
-	[[Category: ~~Canal~~]]	+	[[Category: Large Structures]]
-	[[Category: Robertus~~, J D~~]]	+	[[Category: Robertus JD]]
-	[[Category: Ubhi, D]]	+	[[Category: Ubhi D]]
-	~~[[Category: Cobalamin-independent]]~~	+
-	~~[[Category: Dual tim barrel]]~~	+
-	~~[[Category: Fungal]]~~	+
-	~~[[Category: Methionine synthase]]~~	+
-	~~[[Category: Surface entropy reduction]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 18:02, 4 August 2016

OCA — Thu, 04 Aug 2016 18:02:50 GMT

←Older revision		Revision as of 18:02, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine==		==Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine==
	<StructureSection load='4l6o' size='340' side='right' caption='[[4l6o]], [[Resolution\|resolution]] 1.88Å' scene=''>		<StructureSection load='4l6o' size='340' side='right' caption='[[4l6o]], [[Resolution\|resolution]] 1.88Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CaO19.10083, CaO19.2551, MET6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237561 CANAL])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CaO19.10083, CaO19.2551, MET6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237561 CANAL])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l6o RCSB], [http://www.ebi.ac.uk/pdbsum/4l6o PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6o OCA], [http://pdbe.org/4l6o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4l6o RCSB], [http://www.ebi.ac.uk/pdbsum/4l6o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4l6o ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4l6o" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:42, 25 December 2014

OCA — Thu, 25 Dec 2014 11:42:23 GMT

←Older revision		Revision as of 11:42, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4l6o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canal Canal]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L6O FirstGlance]. <br>		<table><tr><td colspan='2'>[[4l6o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canal Canal]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L6O FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l5z\|4l5z]], [[4l61\|4l61]], [[4l64\|4l64]], [[4l65\|4l65]], [[4l6h\|4l6h]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l5z\|4l5z]], [[4l61\|4l61]], [[4l64\|4l64]], [[4l65\|4l65]], [[4l6h\|4l6h]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CaO19.10083, CaO19.2551, MET6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237561 CANAL])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CaO19.10083, CaO19.2551, MET6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237561 CANAL])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l6o RCSB], [http://www.ebi.ac.uk/pdbsum/4l6o PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l6o RCSB], [http://www.ebi.ac.uk/pdbsum/4l6o PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/METE_CANAL METE_CANAL]] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 17:
	Structural analysis of a fungal methionine synthase with substrates and inhibitors.,Ubhi D, Kago G, Monzingo AF, Robertus JD J Mol Biol. 2014 Apr 17;426(8):1839-47. doi: 10.1016/j.jmb.2014.02.006. Epub 2014, Feb 11. PMID:24524835<ref>PMID:24524835</ref>		Structural analysis of a fungal methionine synthase with substrates and inhibitors.,Ubhi D, Kago G, Monzingo AF, Robertus JD J Mol Biol. 2014 Apr 17;426(8):1839-47. doi: 10.1016/j.jmb.2014.02.006. Epub 2014, Feb 11. PMID:24524835<ref>PMID:24524835</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 23:		Line 25:
	[[Category: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase]]		[[Category: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase]]
	[[Category: Canal]]		[[Category: Canal]]
-	[[Category: Robertus, J D.]]	+	[[Category: Robertus, J D]]
-	[[Category: Ubhi, D.]]	+	[[Category: Ubhi, D]]
	[[Category: Cobalamin-independent]]		[[Category: Cobalamin-independent]]
	[[Category: Dual tim barrel]]		[[Category: Dual tim barrel]]

OCA at 12:15, 18 May 2014

OCA — Sun, 18 May 2014 12:15:55 GMT

←Older revision		Revision as of 12:15, 18 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4l6o\| PDB=4l6o \| SCENE= }}~~	+	==Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine==
-	===Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine===	+	<StructureSection load='4l6o' size='340' side='right' caption='[[4l6o]], [[Resolution\|resolution]] 1.88Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24524835}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4l6o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canal Canal]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L6O FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l5z\|4l5z]], [[4l61\|4l61]], [[4l64\|4l64]], [[4l65\|4l65]], [[4l6h\|4l6h]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CaO19.10083, CaO19.2551, MET6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237561 CANAL])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l6o RCSB], [http://www.ebi.ac.uk/pdbsum/4l6o PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cobalamin-independent methionine synthase from Candida albicans, known as Met6p, is a 90-kDa enzyme that consists of two (betaalpha)8 barrels. The active site is located between the two domains and has binding sites for a zinc ion and substrates l-homocysteine and 5-methyl-tetrahydrofolate-glutamate3. Met6p catalyzes transfer of the methyl group of 5-methyl-tetrahydrofolate-glutamate3 to the l-homocysteine thiolate to generate methionine. Met6p is essential for fungal growth, and we currently pursue it as an antifungal drug design target. Here we report the binding of l-homocysteine, methionine, and several folate analogs. We show that binding of l-homocysteine or methionine results in conformational rearrangements at the amino acid binding pocket, moving the catalytic zinc into position to activate the thiol group. We also map the folate binding pocket and identify specific binding residues, like Asn126, whose mutation eliminates catalytic activity. We also report the development of a robust fluorescence-based activity assay suitable for high-throughput screening. We use this assay and an X-ray structure to characterize methotrexate as a weak inhibitor of fungal Met6p.

-	~~==Function==~~	+	Structural analysis of a fungal methionine synthase with substrates and inhibitors.,Ubhi D, Kago G, Monzingo AF, Robertus JD J Mol Biol. 2014 Apr 17;426(8):1839-47. doi: 10.1016/j.jmb.2014.02.006. Epub 2014, Feb 11. PMID:24524835<ref>PMID:24524835</ref>
-	~~[[http://www.uniprot.org/uniprot/METE_CANAL METE_CANAL]] Catalyzes the transfer~~ of a ~~methyl group from 5-methyltetrahydrofolate to homocysteine resulting in~~ methionine ~~formation~~.~~[HAMAP~~-~~Rule~~:~~MF_00172]~~	+

-	~~==About this Structure==~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4l6o]] is~~ a ~~1 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4L6O OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:024524835</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase]]		[[Category: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase]]
		+	[[Category: Canal]]
	[[Category: Robertus, J D.]]		[[Category: Robertus, J D.]]
	[[Category: Ubhi, D.]]		[[Category: Ubhi, D.]]

OCA at 04:49, 9 April 2014

OCA — Wed, 09 Apr 2014 04:49:01 GMT

←Older revision		Revision as of 04:49, 9 April 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4l6o\| PDB=4l6o \| SCENE= }}
		+	===Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine===
		+	{{ABSTRACT_PUBMED_24524835}}

-	~~The entry 4l6o is ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/METE_CANAL METE_CANAL]] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]

-	~~Authors~~: ~~Ubhi, D~~.~~, Robertus, J~~.D.	+	==About this Structure==
		+	[[4l6o]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L6O OCA].

-	~~Description~~: ~~Crystal structure of the Candida albicans~~ Methionine ~~Synthase in complex with Glutamine~~	+	==Reference==
		+	<ref group="xtra">PMID:024524835</ref><references group="xtra"/><references/>
		+	[[Category: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase]]
		+	[[Category: Robertus, J D.]]
		+	[[Category: Ubhi, D.]]
		+	[[Category: Cobalamin-independent]]
		+	[[Category: Dual tim barrel]]
		+	[[Category: Fungal]]
		+	[[Category: Methionine synthase]]
		+	[[Category: Surface entropy reduction]]
		+	[[Category: Transferase]]

OCA at 05:53, 23 October 2013

OCA — Wed, 23 Oct 2013 05:53:40 GMT

←Older revision		Revision as of 05:53, 23 October 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4l6o is ON HOLD	+	The entry 4l6o is ON HOLD until Paper Publication

	Authors: Ubhi, D., Robertus, J.D.		Authors: Ubhi, D., Robertus, J.D.

	Description: Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine		Description: Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine

OCA at 04:14, 1 August 2013

OCA — Thu, 01 Aug 2013 04:14:38 GMT

←Older revision		Revision as of 04:14, 1 August 2013
Line 3:		Line 3:
	The entry 4l6o is ON HOLD		The entry 4l6o is ON HOLD

-	Authors: Ubhi, D, Robertus, J. D	+	Authors: Ubhi, D., Robertus, J.D.

	Description: Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine		Description: Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine

OCA: Protected "4l6o" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2013 12:36:37 GMT

Protected "4l6o" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:36, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4l6o is ON HOLD Authors: Ubhi, D, Robertus, J. D Description: Crystal structure of the Candida albicans Methionine Synthase in complex with Glutami...

OCA — Wed, 19 Jun 2013 12:36:35 GMT

New page: '''Unreleased structure''' The entry 4l6o is ON HOLD Authors: Ubhi, D, Robertus, J. D Description: Crystal structure of the Candida albicans Methionine Synthase in complex with Glutami...

New page

'''Unreleased structure'''

The entry 4l6o is ON HOLD

Authors: Ubhi, D, Robertus, J. D

Description: Crystal structure of the Candida albicans Methionine Synthase in complex with Glutamine