4l8f - Revision history

OCA at 14:33, 8 November 2023

OCA — Wed, 08 Nov 2023 14:33:09 GMT

←Older revision		Revision as of 14:33, 8 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4l8f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L8F FirstGlance]. <br>		<table><tr><td colspan='2'>[[4l8f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L8F FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MTX:METHOTREXATE'>MTX</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.97Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MTX:METHOTREXATE'>MTX</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8f OCA], [https://pdbe.org/4l8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l8f RCSB], [https://www.ebi.ac.uk/pdbsum/4l8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8f OCA], [https://pdbe.org/4l8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l8f RCSB], [https://www.ebi.ac.uk/pdbsum/4l8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8f ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:44, 14 December 2022

OCA — Wed, 14 Dec 2022 10:44:14 GMT

←Older revision		Revision as of 10:44, 14 December 2022
Line 3:		Line 3:
	<StructureSection load='4l8f' size='340' side='right'caption='[[4l8f]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='4l8f' size='340' side='right'caption='[[4l8f]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4l8f]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Brachidanio_rerio Brachidanio~~ rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4L8F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4l8f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L8F FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTX:METHOTREXATE'>MTX</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MTX:METHOTREXATE'>MTX</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l7q\|4l7q]], [[4l8w\|4l8w]], [[4l8y\|4l8y]], [[4l95\|4l95]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8f OCA], [https://pdbe.org/4l8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l8f RCSB], [https://www.ebi.ac.uk/pdbsum/4l8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8f ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ggh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gamma-glutamyl_hydrolase Gamma-glutamyl hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.9 3.4.19.9] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8f OCA], [~~http~~://pdbe.org/4l8f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4l8f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4l8f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8f ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6NY42_DANRE Q6NY42_DANRE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Brachidanio~~ rerio]]	+	[[Category: Danio rerio]]
-	~~[[Category: Gamma-glutamyl hydrolase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen, C J]]	+	[[Category: Chen C-J]]
-	[[Category: Chuankhayan, P]]	+	[[Category: Chuankhayan P]]
-	[[Category: Fu, T F]]	+	[[Category: Fu T-F]]
-	[[Category: Kao, T T]]	+	[[Category: Kao T-T]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Sandwiched-like domain~~]]	+

OCA at 09:34, 3 April 2019

OCA — Wed, 03 Apr 2019 09:34:20 GMT

←Older revision		Revision as of 09:34, 3 April 2019
Line 1:		Line 1:

	==Crystal structure of gamma-glutamyl hydrolase (C108A) complex with MTX==		==Crystal structure of gamma-glutamyl hydrolase (C108A) complex with MTX==
-	<StructureSection load='4l8f' size='340' side='right' caption='[[4l8f]], [[Resolution\|resolution]] 1.97Å' scene=''>	+	<StructureSection load='4l8f' size='340' side='right'caption='[[4l8f]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4l8f]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L8F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4l8f]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L8F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTX:METHOTREXATE'>MTX</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTX:METHOTREXATE'>MTX</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l7q\|4l7q]], [[4l8w\|4l8w]], [[4l8y\|4l8y]], [[4l95\|4l95]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l7q\|4l7q]], [[4l8w\|4l8w]], [[4l8y\|4l8y]], [[4l95\|4l95]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ggh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gamma-glutamyl_hydrolase Gamma-glutamyl hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.9 3.4.19.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gamma-glutamyl_hydrolase Gamma-glutamyl hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.9 3.4.19.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8f OCA], [http://pdbe.org/4l8f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4l8f RCSB], [http://www.ebi.ac.uk/pdbsum/4l8f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8f OCA], [http://pdbe.org/4l8f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4l8f RCSB], [http://www.ebi.ac.uk/pdbsum/4l8f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8f ProSAT]</span></td></tr>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Brachidanio rerio]]
	[[Category: Gamma-glutamyl hydrolase]]		[[Category: Gamma-glutamyl hydrolase]]
		+	[[Category: Large Structures]]
	[[Category: Chen, C J]]		[[Category: Chen, C J]]
	[[Category: Chuankhayan, P]]		[[Category: Chuankhayan, P]]

OCA at 18:25, 11 August 2016

OCA — Thu, 11 Aug 2016 18:25:14 GMT

←Older revision		Revision as of 18:25, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of gamma-glutamyl hydrolase (C108A) complex with MTX==		==Crystal structure of gamma-glutamyl hydrolase (C108A) complex with MTX==
	<StructureSection load='4l8f' size='340' side='right' caption='[[4l8f]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='4l8f' size='340' side='right' caption='[[4l8f]], [[Resolution\|resolution]] 1.97Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l7q\|4l7q]], [[4l8w\|4l8w]], [[4l8y\|4l8y]], [[4l95\|4l95]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l7q\|4l7q]], [[4l8w\|4l8w]], [[4l8y\|4l8y]], [[4l95\|4l95]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gamma-glutamyl_hydrolase Gamma-glutamyl hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.9 3.4.19.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gamma-glutamyl_hydrolase Gamma-glutamyl hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.9 3.4.19.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l8f RCSB], [http://www.ebi.ac.uk/pdbsum/4l8f PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8f OCA], [http://pdbe.org/4l8f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4l8f RCSB], [http://www.ebi.ac.uk/pdbsum/4l8f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8f ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4l8f" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:03, 5 January 2015

OCA — Mon, 05 Jan 2015 09:03:41 GMT

←Older revision		Revision as of 09:03, 5 January 2015
Line 2:		Line 2:
	<StructureSection load='4l8f' size='340' side='right' caption='[[4l8f]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='4l8f' size='340' side='right' caption='[[4l8f]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4l8f]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8F OCA]. <br>	+	<table><tr><td colspan='2'>[[4l8f]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L8F FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTX:METHOTREXATE'>MTX</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTX:METHOTREXATE'>MTX</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l7q\|4l7q]], [[4l8w\|4l8w]], [[4l8y\|4l8y]], [[4l95\|4l95]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l7q\|4l7q]], [[4l8w\|4l8w]], [[4l8y\|4l8y]], [[4l95\|4l95]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Glucokinase Glucokinase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.~~2 2~~.7.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gamma-glutamyl_hydrolase Gamma-glutamyl hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.9 3.4.19.9] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l8f RCSB], [http://www.ebi.ac.uk/pdbsum/4l8f PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l8f RCSB], [http://www.ebi.ac.uk/pdbsum/4l8f PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Structural insights into the hydrolysis and polymorphism of methotrexate polyglutamate by zebrafish gamma-glutamyl hydrolase.,Chuankhayan P, Kao TT, Lin CC, Guan HH, Nakagawa A, Fu TF, Chen CJ J Med Chem. 2013 Oct 10;56(19):7625-35. doi: 10.1021/jm401013e. Epub 2013 Sep 27. PMID:24028568<ref>PMID:24028568</ref>		Structural insights into the hydrolysis and polymorphism of methotrexate polyglutamate by zebrafish gamma-glutamyl hydrolase.,Chuankhayan P, Kao TT, Lin CC, Guan HH, Nakagawa A, Fu TF, Chen CJ J Med Chem. 2013 Oct 10;56(19):7625-35. doi: 10.1021/jm401013e. Epub 2013 Sep 27. PMID:24028568<ref>PMID:24028568</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Gamma-glutamyl hydrolase]]		[[Category: Gamma-glutamyl hydrolase]]
-	[[Category: Chen, C J.]]	+	[[Category: Chen, C J]]
-	[[Category: Chuankhayan, P.]]	+	[[Category: Chuankhayan, P]]
-	[[Category: Fu, T F.]]	+	[[Category: Fu, T F]]
-	[[Category: Kao, T T~~.]]~~	+	[[Category: Kao, T T]]
-	~~[[Category: Gamma-glutamyl hydrolase~~]]	+
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Sandwiched-like domain]]		[[Category: Sandwiched-like domain]]

OCA at 07:16, 14 May 2014

OCA — Wed, 14 May 2014 07:16:43 GMT

←Older revision		Revision as of 07:16, 14 May 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of gamma-glutamyl hydrolase (C108A) complex with MTX==
		+	<StructureSection load='4l8f' size='340' side='right' caption='[[4l8f]], [[Resolution\|resolution]] 1.97Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4l8f]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8F OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTX:METHOTREXATE'>MTX</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4l7q\|4l7q]], [[4l8w\|4l8w]], [[4l8y\|4l8y]], [[4l95\|4l95]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l8f RCSB], [http://www.ebi.ac.uk/pdbsum/4l8f PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	gamma-Glutamyl hydrolases (gammaGH) catalyze the hydrolysis of gamma-linked glutamate residues from the polyglutamyl of folates and antifolates, such as methotrexate (MTX), a widely used anticancer drug. We describe the first crystal structures of the endopeptidase-type gammaGH (zgammaGH) from zebrafish and the mutant complexes with MTX(Glu)5 and hydrolyzed MTX(Glu)1, revealing the complete set of key residues involved in hydrolysis as well as the substrate-binding subsites (-1 to +2). The side chain of Phe20 and the 6-methylpterin ring of MTX(Glu)5 invoke pi-pi interactions to promote distinct concerted conformational alterations involving approximately 90 degrees rotations in the complexes with the zgammaGH-C108A and zgammaGH-H218N mutant proteins. The structural geometries of the MTX(Glu)5 and hydrolyzed MTX(Glu)1 in the mutant complexes differ significantly from those of the previously known MTX(Glu)1, providing polymorphic information. Together with the structural comparison and the activity analysis, these results shed light on the catalytic mechanism and substrate recognition of zgammaGH and other gamma-glutamyl hydrolases.

-	~~The entry 4l8f is ON HOLD until Paper Publication~~	+	Structural insights into the hydrolysis and polymorphism of methotrexate polyglutamate by zebrafish gamma-glutamyl hydrolase.,Chuankhayan P, Kao TT, Lin CC, Guan HH, Nakagawa A, Fu TF, Chen CJ J Med Chem. 2013 Oct 10;56(19):7625-35. doi: 10.1021/jm401013e. Epub 2013 Sep 27. PMID:24028568<ref>PMID:24028568</ref>

-	~~Authors: Chuankhayan~~, P.~~, Kao, T~~.-T., Chen, C.-J., Fu, T.-F.	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of gamma~~-glutamyl hydrolase ~~(C108A) complex with MTX~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gamma-glutamyl hydrolase]]
		+	[[Category: Chen, C J.]]
		+	[[Category: Chuankhayan, P.]]
		+	[[Category: Fu, T F.]]
		+	[[Category: Kao, T T.]]
		+	[[Category: Gamma-glutamyl hydrolase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Sandwiched-like domain]]

OCA at 13:21, 3 July 2013

OCA — Wed, 03 Jul 2013 13:21:23 GMT

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-	The entry 4l8f is ON HOLD	+	The entry 4l8f is ON HOLD until Paper Publication

	Authors: Chuankhayan, P., Kao, T.-T., Chen, C.-J., Fu, T.-F.		Authors: Chuankhayan, P., Kao, T.-T., Chen, C.-J., Fu, T.-F.

	Description: Crystal structure of gamma-glutamyl hydrolase (C108A) complex with MTX		Description: Crystal structure of gamma-glutamyl hydrolase (C108A) complex with MTX

OCA: Protected "4l8f" [edit=sysop:move=sysop]

OCA — Sun, 30 Jun 2013 09:02:57 GMT

Protected "4l8f" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:02, 30 June 2013

OCA: New page: '''Unreleased structure''' The entry 4l8f is ON HOLD Authors: Chuankhayan, P., Kao, T.-T., Chen, C.-J., Fu, T.-F. Description: Crystal structure of gamma-glutamyl hydrolase (C108A) com...

OCA — Sun, 30 Jun 2013 09:02:54 GMT

New page: '''Unreleased structure''' The entry 4l8f is ON HOLD Authors: Chuankhayan, P., Kao, T.-T., Chen, C.-J., Fu, T.-F. Description: Crystal structure of gamma-glutamyl hydrolase (C108A) com...

New page

'''Unreleased structure'''

The entry 4l8f is ON HOLD

Authors: Chuankhayan, P., Kao, T.-T., Chen, C.-J., Fu, T.-F.

Description: Crystal structure of gamma-glutamyl hydrolase (C108A) complex with MTX