4l8i - Revision history

OCA at 12:18, 1 March 2024

OCA — Fri, 01 Mar 2024 12:18:06 GMT

←Older revision		Revision as of 12:18, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4l8i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L8I FirstGlance]. <br>		<table><tr><td colspan='2'>[[4l8i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L8I FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8i OCA], [https://pdbe.org/4l8i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l8i RCSB], [https://www.ebi.ac.uk/pdbsum/4l8i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8i ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8i OCA], [https://pdbe.org/4l8i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l8i RCSB], [https://www.ebi.ac.uk/pdbsum/4l8i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8i ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Vaccines prevent infectious disease largely by inducing protective neutralizing antibodies against vulnerable epitopes. Several major pathogens have resisted traditional vaccine development, although vulnerable epitopes targeted by neutralizing antibodies have been identified for several such cases. Hence, new vaccine design methods to induce epitope-specific neutralizing antibodies are needed. Here we show, with a neutralization epitope from respiratory syncytial virus, that computational protein design can generate small, thermally and conformationally stable protein scaffolds that accurately mimic the viral epitope structure and induce potent neutralizing antibodies. These scaffolds represent promising leads for the research and development of a human respiratory syncytial virus vaccine needed to protect infants, young children and the elderly. More generally, the results provide proof of principle for epitope-focused and scaffold-based vaccine design, and encourage the evaluation and further development of these strategies for a variety of other vaccine targets, including antigenically highly variable pathogens such as human immunodeficiency virus and influenza.
-
-	Proof of principle for epitope-focused vaccine design.,Correia BE, Bates JT, Loomis RJ, Baneyx G, Carrico C, Jardine JG, Rupert P, Correnti C, Kalyuzhniy O, Vittal V, Connell MJ, Stevens E, Schroeter A, Chen M, Macpherson S, Serra AM, Adachi Y, Holmes MA, Li Y, Klevit RE, Graham BS, Wyatt RT, Baker D, Strong RK, Crowe JE, Johnson PR, Schief WR Nature. 2014 Feb 5. doi: 10.1038/nature12966. PMID:24499818<ref>PMID:24499818</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4l8i" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:44, 14 December 2022

OCA — Wed, 14 Dec 2022 10:44:20 GMT

←Older revision		Revision as of 10:44, 14 December 2022
Line 3:		Line 3:
	<StructureSection load='4l8i' size='340' side='right'caption='[[4l8i]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4l8i' size='340' side='right'caption='[[4l8i]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4l8i]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4L8I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4l8i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L8I FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4l8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8i OCA], [~~http~~://pdbe.org/4l8i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4l8i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4l8i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8i ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8i OCA], [https://pdbe.org/4l8i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l8i RCSB], [https://www.ebi.ac.uk/pdbsum/4l8i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8i ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Correnti, C]]	+	[[Category: Correnti C]]
-	[[Category: Holmes~~, M A~~]]	+	[[Category: Holmes MA]]
-	[[Category: Jardine, J]]	+	[[Category: Jardine J]]
-	[[Category: Schief~~, W R~~]]	+	[[Category: Schief WR]]
-	[[Category: Strong~~, R K]]~~	+	[[Category: Strong RK]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Three-helix bundle~~]]	+

OCA at 05:30, 13 February 2020

OCA — Thu, 13 Feb 2020 05:30:38 GMT

←Older revision		Revision as of 05:30, 13 February 2020
Line 1:		Line 1:

	==Crystal structure of RSV epitope scaffold FFL_005==		==Crystal structure of RSV epitope scaffold FFL_005==
-	<StructureSection load='4l8i' size='340' side='right' caption='[[4l8i]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4l8i' size='340' side='right'caption='[[4l8i]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4l8i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L8I FirstGlance]. <br>		<table><tr><td colspan='2'>[[4l8i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L8I FirstGlance]. <br>
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Synthetic construct sequences]]		[[Category: Synthetic construct sequences]]
	[[Category: Correnti, C]]		[[Category: Correnti, C]]

OCA at 14:54, 11 August 2016

OCA — Thu, 11 Aug 2016 14:54:16 GMT

←Older revision		Revision as of 14:54, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of RSV epitope scaffold FFL_005==		==Crystal structure of RSV epitope scaffold FFL_005==
	<StructureSection load='4l8i' size='340' side='right' caption='[[4l8i]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4l8i' size='340' side='right' caption='[[4l8i]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4l8i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L8I FirstGlance]. <br>		<table><tr><td colspan='2'>[[4l8i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L8I FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l8i RCSB], [http://www.ebi.ac.uk/pdbsum/4l8i PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8i OCA], [http://pdbe.org/4l8i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4l8i RCSB], [http://www.ebi.ac.uk/pdbsum/4l8i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8i ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 13:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4l8i" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:05, 5 January 2015

OCA — Mon, 05 Jan 2015 09:05:35 GMT

←Older revision		Revision as of 09:05, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4l8i\| PDB=4l8i \| SCENE= }}~~	+	==Crystal structure of RSV epitope scaffold FFL_005==
-	===Crystal structure of RSV epitope scaffold FFL_005===	+	<StructureSection load='4l8i' size='340' side='right' caption='[[4l8i]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24499818}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4l8i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L8I FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l8i RCSB], [http://www.ebi.ac.uk/pdbsum/4l8i PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Vaccines prevent infectious disease largely by inducing protective neutralizing antibodies against vulnerable epitopes. Several major pathogens have resisted traditional vaccine development, although vulnerable epitopes targeted by neutralizing antibodies have been identified for several such cases. Hence, new vaccine design methods to induce epitope-specific neutralizing antibodies are needed. Here we show, with a neutralization epitope from respiratory syncytial virus, that computational protein design can generate small, thermally and conformationally stable protein scaffolds that accurately mimic the viral epitope structure and induce potent neutralizing antibodies. These scaffolds represent promising leads for the research and development of a human respiratory syncytial virus vaccine needed to protect infants, young children and the elderly. More generally, the results provide proof of principle for epitope-focused and scaffold-based vaccine design, and encourage the evaluation and further development of these strategies for a variety of other vaccine targets, including antigenically highly variable pathogens such as human immunodeficiency virus and influenza.

-	~~==About this Structure==~~	+	Proof of principle for epitope-focused vaccine design.,Correia BE, Bates JT, Loomis RJ, Baneyx G, Carrico C, Jardine JG, Rupert P, Correnti C, Kalyuzhniy O, Vittal V, Connell MJ, Stevens E, Schroeter A, Chen M, Macpherson S, Serra AM, Adachi Y, Holmes MA, Li Y, Klevit RE, Graham BS, Wyatt RT, Baker D, Strong RK, Crowe JE, Johnson PR, Schief WR Nature. 2014 Feb 5. doi: 10.1038/nature12966. PMID:24499818<ref>PMID:24499818</ref>
-	~~[[4l8i]] is a 2 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4L8I OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024499818</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
-	[[Category: Correnti, C.]]	+	== References ==
-	[[Category: Holmes, M A.]]	+	<references/>
-	[[Category: Jardine, J.]]	+	__TOC__
-	[[Category: Schief, W R.]]	+	</StructureSection>
-	[[Category: Strong, R K.]]	+	[[Category: Synthetic construct sequences]]
		+	[[Category: Correnti, C]]
		+	[[Category: Holmes, M A]]
		+	[[Category: Jardine, J]]
		+	[[Category: Schief, W R]]
		+	[[Category: Strong, R K]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Three-helix bundle]]		[[Category: Three-helix bundle]]

OCA at 09:03, 19 March 2014

OCA — Wed, 19 Mar 2014 09:03:11 GMT

←Older revision		Revision as of 09:03, 19 March 2014
Line 1:		Line 1:
	{{STRUCTURE_4l8i\| PDB=4l8i \| SCENE= }}		{{STRUCTURE_4l8i\| PDB=4l8i \| SCENE= }}
	===Crystal structure of RSV epitope scaffold FFL_005===		===Crystal structure of RSV epitope scaffold FFL_005===
		+	{{ABSTRACT_PUBMED_24499818}}

	==About this Structure==		==About this Structure==
	[[4l8i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA].		[[4l8i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024499818</ref><references group="xtra"/><references/>
	[[Category: Correnti, C.]]		[[Category: Correnti, C.]]
	[[Category: Holmes, M A.]]		[[Category: Holmes, M A.]]

OCA at 05:18, 13 February 2014

OCA — Thu, 13 Feb 2014 05:18:25 GMT

←Older revision		Revision as of 05:18, 13 February 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4l8i\| PDB=4l8i \| SCENE= }}
		+	===Crystal structure of RSV epitope scaffold FFL_005===

-	~~The entry~~ 4l8i is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4l8i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8I OCA].
-	~~Authors~~: ~~Jardine, J~~., Correnti, C., Holmes, M.A., ~~Strong, R~~.~~K.,~~ Schief, W.R.	+	[[Category: Correnti, C.]]
-		+	[[Category: Holmes, M A.]]
-	~~Description~~: ~~Crystal structure of RSV epitope scaffold FFL_005~~	+	[[Category: Jardine, J.]]
		+	[[Category: Schief, W R.]]
		+	[[Category: Strong, R K.]]
		+	[[Category: Immune system]]
		+	[[Category: Three-helix bundle]]

OCA at 13:21, 3 July 2013

OCA — Wed, 03 Jul 2013 13:21:30 GMT

←Older revision		Revision as of 13:21, 3 July 2013
Line 3:		Line 3:
	The entry 4l8i is ON HOLD		The entry 4l8i is ON HOLD

-	Authors: Jardine, J, Correnti, C, Holmes, M.A., Strong, R.K., Schief, W.R.	+	Authors: Jardine, J., Correnti, C., Holmes, M.A., Strong, R.K., Schief, W.R.

	Description: Crystal structure of RSV epitope scaffold FFL_005		Description: Crystal structure of RSV epitope scaffold FFL_005

OCA: Protected "4l8i" [edit=sysop:move=sysop]

OCA — Sun, 30 Jun 2013 09:03:10 GMT

Protected "4l8i" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:03, 30 June 2013

OCA: New page: '''Unreleased structure''' The entry 4l8i is ON HOLD Authors: Jardine, J, Correnti, C, Holmes, M.A., Strong, R.K., Schief, W.R. Description: Crystal structure of RSV epitope scaffold F...

OCA — Sun, 30 Jun 2013 09:03:09 GMT

New page: '''Unreleased structure''' The entry 4l8i is ON HOLD Authors: Jardine, J, Correnti, C, Holmes, M.A., Strong, R.K., Schief, W.R. Description: Crystal structure of RSV epitope scaffold F...

New page

'''Unreleased structure'''

The entry 4l8i is ON HOLD

Authors: Jardine, J, Correnti, C, Holmes, M.A., Strong, R.K., Schief, W.R.

Description: Crystal structure of RSV epitope scaffold FFL_005