4la6 - Revision history

OCA at 16:15, 20 September 2023

OCA — Wed, 20 Sep 2023 16:15:23 GMT

←Older revision		Revision as of 16:15, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4la6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LA6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4la6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LA6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LA6:(2E)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>LA6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.001Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LA6:(2E)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>LA6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4la6 OCA], [https://pdbe.org/4la6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4la6 RCSB], [https://www.ebi.ac.uk/pdbsum/4la6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4la6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4la6 OCA], [https://pdbe.org/4la6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4la6 RCSB], [https://www.ebi.ac.uk/pdbsum/4la6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4la6 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:47, 14 December 2022

OCA — Wed, 14 Dec 2022 10:47:39 GMT

←Older revision		Revision as of 10:47, 14 December 2022
Line 1:		Line 1:

	==Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluoroneryl diphosphate==		==Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluoroneryl diphosphate==
-	<StructureSection load='4la6' size='340' side='right' caption='[[4la6]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4la6' size='340' side='right'caption='[[4la6]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4la6]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strco Strco~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LA6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4LA6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4la6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LA6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LA6:(2E)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>LA6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LA6:(2E)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>LA6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v1v\|3v1v]], [[3v1x\|3v1x]], [[4la5\|4la5]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4la6 OCA], [https://pdbe.org/4la6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4la6 RCSB], [https://www.ebi.ac.uk/pdbsum/4la6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4la6 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-methylisoborneol_synthase 2-methylisoborneol synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.118 4.2.3.118] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4la6 OCA], [~~http~~://pdbe.org/4la6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4la6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4la6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4la6 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MIBS_STRCO MIBS_STRCO]] Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the intermediacy of 2-methyllinalyl diphosphate. Is also able to catalyze the cyclization of geranyl diphosphate (GPP), albeit with much lower efficiency, leading to the formation of a complex mixture of cyclic monoterpenes, consisting of alpha-pinene (6%), beta-pinene (23%), limonene (32%), gamma-terpinene (29%), and delta-terpinene (10%).	+	[https://www.uniprot.org/uniprot/MIBS_STRCO MIBS_STRCO] Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the intermediacy of 2-methyllinalyl diphosphate. Is also able to catalyze the cyclization of geranyl diphosphate (GPP), albeit with much lower efficiency, leading to the formation of a complex mixture of cyclic monoterpenes, consisting of alpha-pinene (6%), beta-pinene (23%), limonene (32%), gamma-terpinene (29%), and delta-terpinene (10%).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~2-methylisoborneol synthase]]~~	+	[[Category: Large Structures]]
-	~~[[Category: Strco~~]]	+	[[Category: Christianson DW]]
-	[[Category: Christianson~~, D W~~]]	+	[[Category: Koksal M]]
-	[[Category: Koksal, M]]	+
-	~~[[Category: Biosynthesis]]~~	+
-	~~[[Category: Isoprenoid synthase fold]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Terpenoid biosynthesis~~]]	+

OCA at 04:17, 5 August 2016

OCA — Fri, 05 Aug 2016 04:17:41 GMT

←Older revision		Revision as of 04:17, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluoroneryl diphosphate==		==Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluoroneryl diphosphate==
	<StructureSection load='4la6' size='340' side='right' caption='[[4la6]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4la6' size='340' side='right' caption='[[4la6]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-methylisoborneol_synthase 2-methylisoborneol synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.118 4.2.3.118] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-methylisoborneol_synthase 2-methylisoborneol synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.118 4.2.3.118] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4la6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4la6 RCSB], [http://www.ebi.ac.uk/pdbsum/4la6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4la6 OCA], [http://pdbe.org/4la6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4la6 RCSB], [http://www.ebi.ac.uk/pdbsum/4la6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4la6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4la6" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:53, 24 December 2014

OCA — Wed, 24 Dec 2014 22:53:38 GMT

←Older revision		Revision as of 22:53, 24 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4la6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4la6 RCSB], [http://www.ebi.ac.uk/pdbsum/4la6 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4la6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4la6 RCSB], [http://www.ebi.ac.uk/pdbsum/4la6 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MIBS_STRCO MIBS_STRCO]] Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the intermediacy of 2-methyllinalyl diphosphate. Is also able to catalyze the cyclization of geranyl diphosphate (GPP), albeit with much lower efficiency, leading to the formation of a complex mixture of cyclic monoterpenes, consisting of alpha-pinene (6%), beta-pinene (23%), limonene (32%), gamma-terpinene (29%), and delta-terpinene (10%).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:08, 21 December 2014

OCA — Sun, 21 Dec 2014 18:08:38 GMT

←Older revision		Revision as of 18:08, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4la6~~\| ~~PDB~~=4la6 \| ~~SCENE~~= }}	+	==Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluoroneryl diphosphate==
-	===~~Crystal~~ structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluoroneryl diphosphate~~===~~	+	<StructureSection load='4la6' size='340' side='right' caption='[[4la6]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23844678}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4la6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LA6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LA6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LA6:(2E)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>LA6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v1v\|3v1v]], [[3v1x\|3v1x]], [[4la5\|4la5]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-methylisoborneol_synthase 2-methylisoborneol synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.118 4.2.3.118] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4la6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4la6 RCSB], [http://www.ebi.ac.uk/pdbsum/4la6 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of 2-methylisoborneol synthase (MIBS) from Streptomyces coelicolor A3(2) has been determined in its unliganded state and in complex with two Mg2+ ions and 2-fluoroneryl diphosphate at 1.85 and 2.00 A resolution, respectively. Under normal circumstances, MIBS catalyzes the cyclization of the naturally occurring, noncanonical 11-carbon isoprenoid substrate, 2-methylgeranyl diphosphate, which first undergoes an ionization-isomerization-ionization sequence through the tertiary diphosphate intermediate 2-methyllinalyl diphosphate to enable subsequent cyclization chemistry. MIBS does not exhibit catalytic activity with 2-fluorogeranyl diphosphate, and we recently reported the crystal structure of MIBS complexed with this unreactive substrate analogue [ Koksal, M., Chou, W. K. W., Cane, D. E., Christianson, D. W. (2012) Biochemistry 51 , 3011-3020 ]. However, cocrystallization of MIBS with the fluorinated analogue of the tertiary allylic diphosphate intermediate, 2-fluorolinalyl diphosphate, reveals unexpected reactivity for the intermediate analogue and yields the crystal structure of the complex with the primary allylic diphosphate, 2-fluoroneryl diphosphate. Comparison with the structure of the unliganded enzyme reveals that the crystalline enzyme active site remains partially open, presumably due to the binding of only two Mg2+ ions. Assays in solution indicate that MIBS catalyzes the generation of (1R)-(+)-camphor from the substrate 2-fluorolinalyl diphosphate, suggesting that both 2-fluorolinalyl diphosphate and 2-methyllinalyl diphosphate follow the identical cyclization mechanism leading to 2-substituted isoborneol products; however, the initially generated 2-fluoroisoborneol cyclization product is unstable and undergoes elimination of hydrogen fluoride to yield (1R)-(+)-camphor.

-	~~==Function==~~	+	Unexpected Reactivity of 2-Fluorolinalyl Diphosphate in the Active Site of Crystalline 2-Methylisoborneol Synthase.,Koksal M, Chou WK, Cane DE, Christianson DW Biochemistry. 2013 Jul 22. PMID:23844678<ref>PMID:23844678</ref>
-	~~[[http://www.uniprot.org/uniprot/MIBS_STRCO MIBS_STRCO]] Catalyzes the cyclization~~ of 2-~~methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB), which likely involves~~ the ~~intermediacy~~ of 2-~~methyllinalyl diphosphate~~. ~~Is also able to catalyze the cyclization of geranyl diphosphate (GPP)~~, ~~albeit with much lower efficiency~~, ~~leading to the formation of a complex mixture of cyclic monoterpenes~~, ~~consisting of alpha-pinene (6%)~~, ~~beta-pinene (23%), limonene (32%), gamma-terpinene (29%), and delta-terpinene (10%)~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4la6]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Strco Strco]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4LA6 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023844678</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: 2-methylisoborneol synthase]]		[[Category: 2-methylisoborneol synthase]]
	[[Category: Strco]]		[[Category: Strco]]
-	[[Category: Christianson, D W.]]	+	[[Category: Christianson, D W]]
-	[[Category: Koksal, M.]]	+	[[Category: Koksal, M]]
	[[Category: Biosynthesis]]		[[Category: Biosynthesis]]
	[[Category: Isoprenoid synthase fold]]		[[Category: Isoprenoid synthase fold]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Terpenoid biosynthesis]]		[[Category: Terpenoid biosynthesis]]

OCA at 08:38, 25 December 2013

OCA — Wed, 25 Dec 2013 08:38:41 GMT

←Older revision		Revision as of 08:38, 25 December 2013
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[4la6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_coelicolor_a3(2) Streptomyces coelicolor a3(2)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LA6 OCA].	+	[[4la6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LA6 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:023844678</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:023844678</ref><references group="xtra"/><references/>
	[[Category: 2-methylisoborneol synthase]]		[[Category: 2-methylisoborneol synthase]]
		+	[[Category: Strco]]
	[[Category: Christianson, D W.]]		[[Category: Christianson, D W.]]
	[[Category: Koksal, M.]]		[[Category: Koksal, M.]]

OCA at 05:26, 1 August 2013

OCA — Thu, 01 Aug 2013 05:26:29 GMT

←Older revision		Revision as of 05:26, 1 August 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4la6\| PDB=4la6 \| SCENE= }}
		+	===Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluoroneryl diphosphate===
		+	{{ABSTRACT_PUBMED_23844678}}

-	~~The entry 4la6 is ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/MIBS_STRCO MIBS_STRCO]] Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the intermediacy of 2-methyllinalyl diphosphate. Is also able to catalyze the cyclization of geranyl diphosphate (GPP), albeit with much lower efficiency, leading to the formation of a complex mixture of cyclic monoterpenes, consisting of alpha-pinene (6%), beta-pinene (23%), limonene (32%), gamma-terpinene (29%), and delta-terpinene (10%).

-	~~Authors~~: ~~Koksal, M~~.~~, Christianson, D~~.W.	+	==About this Structure==
		+	[[4la6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor_a3(2) Streptomyces coelicolor a3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LA6 OCA].

-	~~Description~~: ~~Crystal structure of~~ 2-methylisoborneol synthase ~~from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluoroneryl diphosphate~~	+	==Reference==
		+	<ref group="xtra">PMID:023844678</ref><references group="xtra"/><references/>
		+	[[Category: 2-methylisoborneol synthase]]
		+	[[Category: Christianson, D W.]]
		+	[[Category: Koksal, M.]]
		+	[[Category: Biosynthesis]]
		+	[[Category: Isoprenoid synthase fold]]
		+	[[Category: Lyase]]
		+	[[Category: Terpenoid biosynthesis]]

OCA: Protected "4la6" [edit=sysop:move=sysop]

OCA — Sun, 30 Jun 2013 09:10:21 GMT

Protected "4la6" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:10, 30 June 2013

OCA: New page: '''Unreleased structure''' The entry 4la6 is ON HOLD Authors: Koksal, M., Christianson, D.W. Description: Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor ...

OCA — Sun, 30 Jun 2013 09:10:20 GMT

New page: '''Unreleased structure''' The entry 4la6 is ON HOLD Authors: Koksal, M., Christianson, D.W. Description: Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor ...

New page

'''Unreleased structure'''

The entry 4la6 is ON HOLD

Authors: Koksal, M., Christianson, D.W.

Description: Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluoroneryl diphosphate