4lhb - Revision history

OCA at 16:19, 20 September 2023

OCA — Wed, 20 Sep 2023 16:19:12 GMT

←Older revision		Revision as of 16:19, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4lhb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LHB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4lhb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LHB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhb OCA], [https://pdbe.org/4lhb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lhb RCSB], [https://www.ebi.ac.uk/pdbsum/4lhb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lhb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhb OCA], [https://pdbe.org/4lhb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lhb RCSB], [https://www.ebi.ac.uk/pdbsum/4lhb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lhb ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:01, 14 December 2022

OCA — Wed, 14 Dec 2022 11:01:44 GMT

←Older revision		Revision as of 11:01, 14 December 2022
Line 1:		Line 1:

	==Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus==		==Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus==
-	<StructureSection load='4lhb' size='340' side='right' caption='[[4lhb]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4lhb' size='340' side='right'caption='[[4lhb]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4lhb]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pyrfu Pyrfu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4LHB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4lhb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LHB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">moaB, PF0372 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhb OCA], [https://pdbe.org/4lhb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lhb RCSB], [https://www.ebi.ac.uk/pdbsum/4lhb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lhb ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Molybdopterin_adenylyltransferase Molybdopterin adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.75 2.7.7.75] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4lhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhb OCA], [~~http~~://pdbe.org/4lhb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4lhb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4lhb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4lhb ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MOAB_PYRFU MOAB_PYRFU]] Catalyzes the adenylation of molybdopterin as part of the biosynthesis of the molybdenum-cofactor.	+	[https://www.uniprot.org/uniprot/MOAB_PYRFU MOAB_PYRFU] Catalyzes the adenylation of molybdopterin as part of the biosynthesis of the molybdenum-cofactor.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Molybdopterin adenylyltransferase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Pyrfu~~]]	+	[[Category: Pyrococcus furiosus DSM 3638]]
-	[[Category: Havarushka, N]]	+	[[Category: Havarushka N]]
-	[[Category: Schwarz, G]]	+	[[Category: Schwarz G]]
-	~~[[Category: Adenylylation of molybdopterin]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 17:00, 5 August 2016

OCA — Fri, 05 Aug 2016 17:00:17 GMT

←Older revision		Revision as of 17:00, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus==		==Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus==
	<StructureSection load='4lhb' size='340' side='right' caption='[[4lhb]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4lhb' size='340' side='right' caption='[[4lhb]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">moaB, PF0372 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">moaB, PF0372 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Molybdopterin_adenylyltransferase Molybdopterin adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.75 2.7.7.75] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Molybdopterin_adenylyltransferase Molybdopterin adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.75 2.7.7.75] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lhb RCSB], [http://www.ebi.ac.uk/pdbsum/4lhb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhb OCA], [http://pdbe.org/4lhb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lhb RCSB], [http://www.ebi.ac.uk/pdbsum/4lhb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lhb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4lhb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:14, 25 December 2014

OCA — Thu, 25 Dec 2014 04:14:25 GMT

←Older revision		Revision as of 04:14, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4lhb\| PDB=4lhb \| SCENE= }}~~	+	==Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus==
-	===Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus===	+	<StructureSection load='4lhb' size='340' side='right' caption='[[4lhb]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24465852}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4lhb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LHB FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">moaB, PF0372 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Molybdopterin_adenylyltransferase Molybdopterin adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.75 2.7.7.75] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lhb RCSB], [http://www.ebi.ac.uk/pdbsum/4lhb PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/MOAB_PYRFU MOAB_PYRFU]] Catalyzes the adenylation of molybdopterin as part of the biosynthesis of the molybdenum-cofactor.		[[http://www.uniprot.org/uniprot/MOAB_PYRFU MOAB_PYRFU]] Catalyzes the adenylation of molybdopterin as part of the biosynthesis of the molybdenum-cofactor.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Molybdenum and tungsten cofactors share a similar pterin-based scaffold, which hosts an ene-dithiolate function being essential for the coordination of either molybdenum or tungsten. The biosynthesis of both cofactors involves a multistep pathway, which ends with the activation of the metal binding pterin (MPT) by adenylylation before the respective metal is incorporated. In the hyperthermophilic organism Pyrococcus furiosus, the hexameric protein MoaB (PfuMoaB) has been shown to catalyse MPT-adenylylation. Here we determined the crystal structure of PfuMoaB at 2.5 A resolution and identified key residues of alpha3-helix mediating hexamer formation. Given that PfuMoaB homologues from mesophilic organisms form trimers, we investigated the impact on PfuMoaB hexamerization on thermal stability and activity. Using structure-guided mutagenesis, we successfully disrupted the hexamer interface in PfuMoaB. The resulting PfuMoaB-H3 variant formed monomers, dimers and trimers as determined by size exclusion chromatography. Circular dichroism spectroscopy as well as chemical cross-linking coupled to mass spectrometry confirmed a wild-type-like fold of the protomers as well as inter-subunits contacts. The melting temperature of PfuMoaB-H3 was found to be reduced by more than 15 degrees C as determined by differential scanning calorimetry, thus demonstrating hexamerization as key determinant for PfuMoaB thermal stability. Remarkably, while a loss of activity at temperatures higher than 50 degrees C was observed in the PfuMoaB-H3 variant, at lower temperatures, we determined a significantly increased catalytic activity. The latter suggests a gain in conformational flexibility caused by the disruption of the hexamerization interface.

-	~~==About this Structure==~~	+	Structural Basis of Thermal Stability of the Tungsten Cofactor Synthesis Protein MoaB from Pyrococcus furiosus.,Havarushka N, Fischer-Schrader K, Lamkemeyer T, Schwarz G PLoS One. 2014 Jan 20;9(1):e86030. doi: 10.1371/journal.pone.0086030. eCollection, 2014 Jan 20. PMID:24465852<ref>PMID:24465852</ref>
-	~~[[4lhb]] is a 3 chain structure with sequence~~ from ~~[http://en~~.~~wikipedia~~.~~org/wiki/Pyrfu Pyrfu]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4LHB OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024465852</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Molybdopterin adenylyltransferase]]		[[Category: Molybdopterin adenylyltransferase]]
	[[Category: Pyrfu]]		[[Category: Pyrfu]]
-	[[Category: Havarushka, N.]]	+	[[Category: Havarushka, N]]
-	[[Category: Schwarz, G.]]	+	[[Category: Schwarz, G]]
	[[Category: Adenylylation of molybdopterin]]		[[Category: Adenylylation of molybdopterin]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 14:36, 3 March 2014

OCA — Mon, 03 Mar 2014 14:36:21 GMT

←Older revision		Revision as of 14:36, 3 March 2014
Line 1:		Line 1:
	{{STRUCTURE_4lhb\| PDB=4lhb \| SCENE= }}		{{STRUCTURE_4lhb\| PDB=4lhb \| SCENE= }}
	===Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus===		===Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus===
		+	{{ABSTRACT_PUBMED_24465852}}

	==Function==		==Function==
Line 6:		Line 7:

	==About this Structure==		==About this Structure==
-	[[4lhb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHB OCA].	+	[[4lhb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHB OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024465852</ref><references group="xtra"/><references/>
	[[Category: Molybdopterin adenylyltransferase]]		[[Category: Molybdopterin adenylyltransferase]]
-	[[Category: ~~Pyrococcus furiosus dsm 3638~~]]	+	[[Category: Pyrfu]]
	[[Category: Havarushka, N.]]		[[Category: Havarushka, N.]]
	[[Category: Schwarz, G.]]		[[Category: Schwarz, G.]]
	[[Category: Adenylylation of molybdopterin]]		[[Category: Adenylylation of molybdopterin]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 08:01, 14 August 2013

OCA — Wed, 14 Aug 2013 08:01:17 GMT

←Older revision		Revision as of 08:01, 14 August 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4lhb\| PDB=4lhb \| SCENE= }}
		+	===Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus===

-	~~The entry 4lhb is ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/MOAB_PYRFU MOAB_PYRFU]] Catalyzes the adenylation of molybdopterin as part of the biosynthesis of the molybdenum-cofactor.

-	~~Authors~~: Havarushka, N., Schwarz, G.	+	==About this Structure==
-		+	[[4lhb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHB OCA].
-	~~Description~~: ~~Crystal structure~~ of ~~tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus~~	+	[[Category: Molybdopterin adenylyltransferase]]
		+	[[Category: Pyrococcus furiosus dsm 3638]]
		+	[[Category: Havarushka, N.]]
		+	[[Category: Schwarz, G.]]
		+	[[Category: Adenylylation of molybdopterin]]
		+	[[Category: Transferase]]

OCA at 04:46, 18 July 2013

OCA — Thu, 18 Jul 2013 04:46:26 GMT

←Older revision		Revision as of 04:46, 18 July 2013
Line 3:		Line 3:
	The entry 4lhb is ON HOLD		The entry 4lhb is ON HOLD

-	Authors: Havarushka N., Schwarz G.	+	Authors: Havarushka, N., Schwarz, G.

	Description: Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus		Description: Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus

OCA: Protected "4lhb" [edit=sysop:move=sysop]

OCA — Wed, 10 Jul 2013 15:10:25 GMT

Protected "4lhb" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:10, 10 July 2013

OCA: New page: '''Unreleased structure''' The entry 4lhb is ON HOLD Authors: Havarushka N., Schwarz G. Description: Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus fu...

OCA — Wed, 10 Jul 2013 15:10:24 GMT

New page: '''Unreleased structure''' The entry 4lhb is ON HOLD Authors: Havarushka N., Schwarz G. Description: Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus fu...

New page

'''Unreleased structure'''

The entry 4lhb is ON HOLD

Authors: Havarushka N., Schwarz G.

Description: Crystal structure of tungsten cofactor synthesizing protein MoaB from Pyrococcus furiosus