4lv7 - Revision history

OCA at 16:26, 20 September 2023

OCA — Wed, 20 Sep 2023 16:26:45 GMT

←Older revision		Revision as of 16:26, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4lv7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LV7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4lv7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LV7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lv7 OCA], [https://pdbe.org/4lv7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lv7 RCSB], [https://www.ebi.ac.uk/pdbsum/4lv7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lv7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lv7 OCA], [https://pdbe.org/4lv7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lv7 RCSB], [https://www.ebi.ac.uk/pdbsum/4lv7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lv7 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:33, 21 December 2022

OCA — Wed, 21 Dec 2022 10:33:53 GMT

←Older revision		Revision as of 10:33, 21 December 2022
Line 3:		Line 3:
	<StructureSection load='4lv7' size='340' side='right'caption='[[4lv7]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4lv7' size='340' side='right'caption='[[4lv7]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4lv7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LV7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=4LV7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4lv7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LV7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3udz\|3udz]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lv7 OCA], [https://pdbe.org/4lv7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lv7 RCSB], [https://www.ebi.ac.uk/pdbsum/4lv7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lv7 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At5g42810, IPK1, MJB21.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-pentakisphosphate_2-kinase Inositol-pentakisphosphate 2-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.158 2.7.1.158] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=4lv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lv7 OCA], [~~http~~://pdbe.org/4lv7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4lv7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4lv7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4lv7 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/IPPK_ARATH IPPK_ARATH]] Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). Phytate is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Also phosphorylates Ins(1,3,4,6)P4 and Ins(1,4,5,6)P4 to produce Ins(1,2,3,4,6)P5 and Ins(1,2,4,5,6)P5.<ref>PMID:16107538</ref> <ref>PMID:16223361</ref>	+	[https://www.uniprot.org/uniprot/IPPK_ARATH IPPK_ARATH] Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). Phytate is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Also phosphorylates Ins(1,3,4,6)P4 and Ins(1,4,5,6)P4 to produce Ins(1,2,3,4,6)P5 and Ins(1,2,4,5,6)P5.<ref>PMID:16107538</ref> <ref>PMID:16223361</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath]]~~	+	[[Category: Arabidopsis thaliana]]
-	~~[[Category: Inositol-pentakisphosphate 2-kinase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gosein, V]]	+	[[Category: Gosein V]]
-	[[Category: Miller~~, G J]]~~	+	[[Category: Miller GJ]]
-	~~[[Category: Atipk1]]~~	+
-	~~[[Category: Inositol phosphorylation]]~~	+
-	~~[[Category: Ins5p 2-k]]~~	+
-	~~[[Category: Ip5 2-k]]~~	+
-	~~[[Category: Ipk]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 05:56, 14 October 2020

OCA — Wed, 14 Oct 2020 05:56:54 GMT

←Older revision		Revision as of 05:56, 14 October 2020
Line 1:		Line 1:

	==Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C==		==Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C==
-	<StructureSection load='4lv7' size='340' side='right' caption='[[4lv7]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='4lv7' size='340' side='right'caption='[[4lv7]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4lv7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LV7 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4LV7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4lv7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LV7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4LV7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3udz\|3udz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3udz\|3udz]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At5g42810, IPK1, MJB21.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At5g42810, IPK1, MJB21.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-pentakisphosphate_2-kinase Inositol-pentakisphosphate 2-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.158 2.7.1.158] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-pentakisphosphate_2-kinase Inositol-pentakisphosphate 2-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.158 2.7.1.158] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4lv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lv7 OCA], [http://pdbe.org/4lv7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lv7 RCSB], [http://www.ebi.ac.uk/pdbsum/4lv7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lv7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4lv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lv7 OCA], [http://pdbe.org/4lv7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lv7 RCSB], [http://www.ebi.ac.uk/pdbsum/4lv7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lv7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	[[Category: Arath]]		[[Category: Arath]]
	[[Category: Inositol-pentakisphosphate 2-kinase]]		[[Category: Inositol-pentakisphosphate 2-kinase]]
		+	[[Category: Large Structures]]
	[[Category: Gosein, V]]		[[Category: Gosein, V]]
	[[Category: Miller, G J]]		[[Category: Miller, G J]]

OCA at 06:08, 18 April 2018

OCA — Wed, 18 Apr 2018 06:08:41 GMT

←Older revision		Revision as of 06:08, 18 April 2018
Line 12:		Line 12:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/IPPK_ARATH IPPK_ARATH]] Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). Phytate is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Also phosphorylates Ins(1,3,4,6)P4 and Ins(1,4,5,6)P4 to produce Ins(1,2,3,4,6)P5 and Ins(1,2,4,5,6)P5.<ref>PMID:16107538</ref> <ref>PMID:16223361</ref>		[[http://www.uniprot.org/uniprot/IPPK_ARATH IPPK_ARATH]] Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). Phytate is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Also phosphorylates Ins(1,3,4,6)P4 and Ins(1,4,5,6)P4 to produce Ins(1,2,3,4,6)P5 and Ins(1,2,4,5,6)P5.<ref>PMID:16107538</ref> <ref>PMID:16223361</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IPK1) converts inositol 1,3,4,5,6-pentakisphosphate(IP5) to inositol hexakisphosphate (IP6). IPK1 shares structural similarity with protein kinases and is suspected to employ a similar mechanism of activation. Previous studies revealed roles for the 1- and 3-phosphates of IP5 in IPK1 activation and revealed that the N-lobe of IPK1 is unstable in the absence of inositol phosphate (IP). Here, we demonstrate the link between IPK1 substrate specificity and the stability of its N-lobe. Limited proteolysis of IPK1 revealed that N-lobe stability is dependent on the presence of the 1-phosphate of the substrate, whereas overall stability of IPK1 was increased in ternary complexes with nucleotide and IPs possessing 1- and 3-phosphates that engage the N-lobe of IPK1. Thus, the 1- and 3-phosphates possess dual roles in both IPK1 activation and IPK1 stability. To test whether kinase stability directly contributed to substrate selectivity of the kinase, we engineered IPK1 mutants with disulfide bonds that artificially stabilized the N-lobe in an IP-independent manner thereby mimicking its substrate-bound state in the absence of IP. IPK1 E82C/S142C exhibited a DTT-sensitive 5-fold increase in kcat for 3,4,5,6-inositol tetrakisphosphate (3,4,5,6-IP4) as compared with wild-type IPK1. The crystal structure of the IPK1 E82C/S142C mutant confirmed the presence of the disulfide bond and revealed a small shift in the N-lobe. Finally, we determined that IPK1 E82C/S142C is substantially more stable than wild-type IPK1 under nonreducing conditions, revealing that increased stability of IPK1 E82C/S142C correlates with changes in substrate specificity by allowing IPs lacking the stabilizing 1-phosphate to be used. Taken together, our results show that IPK1 substrate selection is linked to the ability of each potential substrate to stabilize IPK1.
		+
		+	Conformational stability of inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IPK1) dictates its substrate selectivity.,Gosein V, Miller GJ J Biol Chem. 2013 Dec 27;288(52):36788-95. doi: 10.1074/jbc.M113.512731. Epub, 2013 Oct 28. PMID:24165122<ref>PMID:24165122</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4lv7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:02, 4 August 2016

OCA — Thu, 04 Aug 2016 11:02:56 GMT

←Older revision		Revision as of 11:02, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C==		==Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C==
	<StructureSection load='4lv7' size='340' side='right' caption='[[4lv7]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4lv7' size='340' side='right' caption='[[4lv7]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4lv7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arabidopsis_thaliana Arabidopsis thaliana~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LV7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LV7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4lv7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LV7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LV7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3udz\|3udz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3udz\|3udz]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At5g42810, IPK1, MJB21.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ~~Arabidopsis thaliana~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At5g42810, IPK1, MJB21.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-pentakisphosphate_2-kinase Inositol-pentakisphosphate 2-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.158 2.7.1.158] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-pentakisphosphate_2-kinase Inositol-pentakisphosphate 2-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.158 2.7.1.158] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lv7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lv7 RCSB], [http://www.ebi.ac.uk/pdbsum/4lv7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lv7 OCA], [http://pdbe.org/4lv7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lv7 RCSB], [http://www.ebi.ac.uk/pdbsum/4lv7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lv7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arabidopsis thaliana~~]]	+	[[Category: Arath]]
	[[Category: Inositol-pentakisphosphate 2-kinase]]		[[Category: Inositol-pentakisphosphate 2-kinase]]
	[[Category: Gosein, V]]		[[Category: Gosein, V]]

OCA at 13:37, 24 December 2014

OCA — Wed, 24 Dec 2014 13:37:37 GMT

←Older revision		Revision as of 13:37, 24 December 2014
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-	~~{{STRUCTURE_4lv7\| PDB=4lv7 \| SCENE= }}~~	+	==Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C==
-	===Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C===	+	<StructureSection load='4lv7' size='340' side='right' caption='[[4lv7]], [[Resolution\|resolution]] 2.60Å' scene=''>
-		+	== Structural highlights ==
-	==Function==	+	<table><tr><td colspan='2'>[[4lv7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LV7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LV7 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3udz\|3udz]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At5g42810, IPK1, MJB21.19 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-pentakisphosphate_2-kinase Inositol-pentakisphosphate 2-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.158 2.7.1.158] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lv7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lv7 RCSB], [http://www.ebi.ac.uk/pdbsum/4lv7 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/IPPK_ARATH IPPK_ARATH]] Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). Phytate is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Also phosphorylates Ins(1,3,4,6)P4 and Ins(1,4,5,6)P4 to produce Ins(1,2,3,4,6)P5 and Ins(1,2,4,5,6)P5.<ref>PMID:16107538</ref> <ref>PMID:16223361</ref>		[[http://www.uniprot.org/uniprot/IPPK_ARATH IPPK_ARATH]] Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). Phytate is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Also phosphorylates Ins(1,3,4,6)P4 and Ins(1,4,5,6)P4 to produce Ins(1,2,3,4,6)P5 and Ins(1,2,4,5,6)P5.<ref>PMID:16107538</ref> <ref>PMID:16223361</ref>
-		+	== References ==
-	==~~About this Structure==~~	+	<references/>
-	[[4lv7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LV7 OCA].	+	__TOC__
-		+	</StructureSection>
-	~~==Reference~~==	+
-	<references ~~group="xtra"~~/><~~references~~/>	+
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Inositol-pentakisphosphate 2-kinase]]		[[Category: Inositol-pentakisphosphate 2-kinase]]
-	[[Category: Gosein, V.]]	+	[[Category: Gosein, V]]
-	[[Category: Miller, G J.]]	+	[[Category: Miller, G J]]
	[[Category: Atipk1]]		[[Category: Atipk1]]
	[[Category: Inositol phosphorylation]]		[[Category: Inositol phosphorylation]]

OCA at 07:51, 6 November 2013

OCA — Wed, 06 Nov 2013 07:51:34 GMT

←Older revision		Revision as of 07:51, 6 November 2013
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-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4lv7\| PDB=4lv7 \| SCENE= }}
		+	===Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C===

-	~~The entry 4lv7~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/IPPK_ARATH IPPK_ARATH]] Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). Phytate is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Also phosphorylates Ins(1,3,4,6)P4 and Ins(1,4,5,6)P4 to produce Ins(1,2,3,4,6)P5 and Ins(1,2,4,5,6)P5.<ref>PMID:16107538</ref> <ref>PMID:16223361</ref>

-	~~Authors~~: ~~Gosein, V~~.~~, Miller, G~~.J.	+	==About this Structure==
		+	[[4lv7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LV7 OCA].

-	~~Description~~: ~~Crystal structure of inositol 1,3,4,5,6~~-pentakisphosphate 2-kinase ~~E82C/S142C~~	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Arabidopsis thaliana]]
		+	[[Category: Inositol-pentakisphosphate 2-kinase]]
		+	[[Category: Gosein, V.]]
		+	[[Category: Miller, G J.]]
		+	[[Category: Atipk1]]
		+	[[Category: Inositol phosphorylation]]
		+	[[Category: Ins5p 2-k]]
		+	[[Category: Ip5 2-k]]
		+	[[Category: Ipk]]
		+	[[Category: Transferase]]

OCA at 06:47, 28 August 2013

OCA — Wed, 28 Aug 2013 06:47:57 GMT

←Older revision		Revision as of 06:47, 28 August 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4lv7 is ON HOLD	+	The entry 4lv7 is ON HOLD until Paper Publication

	Authors: Gosein, V., Miller, G.J.		Authors: Gosein, V., Miller, G.J.

	Description: Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C		Description: Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C

OCA: Protected "4lv7" [edit=sysop:move=sysop]

OCA — Thu, 01 Aug 2013 04:26:26 GMT

Protected "4lv7" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:26, 1 August 2013

OCA: New page: '''Unreleased structure''' The entry 4lv7 is ON HOLD Authors: Gosein, V., Miller, G.J. Description: Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C

OCA — Thu, 01 Aug 2013 04:26:25 GMT

New page: '''Unreleased structure''' The entry 4lv7 is ON HOLD Authors: Gosein, V., Miller, G.J. Description: Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C

New page

'''Unreleased structure'''

The entry 4lv7 is ON HOLD

Authors: Gosein, V., Miller, G.J.

Description: Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase E82C/S142C