4m6g - Revision history

OCA at 16:32, 20 September 2023

OCA — Wed, 20 Sep 2023 16:32:36 GMT

←Older revision		Revision as of 16:32, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4m6g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M6G FirstGlance]. <br>		<table><tr><td colspan='2'>[[4m6g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M6G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=ZGL:D-ALPHA-GLUTAMINE'>ZGL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.104Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=ZGL:D-ALPHA-GLUTAMINE'>ZGL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6g OCA], [https://pdbe.org/4m6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m6g RCSB], [https://www.ebi.ac.uk/pdbsum/4m6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6g OCA], [https://pdbe.org/4m6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m6g RCSB], [https://www.ebi.ac.uk/pdbsum/4m6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6g ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:59, 21 December 2022

OCA — Wed, 21 Dec 2022 10:59:01 GMT

←Older revision		Revision as of 10:59, 21 December 2022
Line 1:		Line 1:

	==Structure of the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product==		==Structure of the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product==
-	<StructureSection load='4m6g' size='340' side='right' caption='[[4m6g]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='4m6g' size='340' side='right'caption='[[4m6g]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4m6g]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4M6G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4m6g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M6G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=ZGL:D-ALPHA-GLUTAMINE'>ZGL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=ZGL:D-ALPHA-GLUTAMINE'>ZGL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m6h\|4m6h]], [[4m6i\|4m6i]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6g OCA], [https://pdbe.org/4m6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m6g RCSB], [https://www.ebi.ac.uk/pdbsum/4m6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6g ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv3717 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4m6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6g OCA], [~~http~~://pdbe.org/4m6g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4m6g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4m6g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6g ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~PEPAM_MYCTU PEPAM_MYCTU~~]] Cell-wall hydrolase that hydrolyzes the amide bond between N-acetylmuramic acid and L-alanine in cell-wall glycopeptides (PubMed:24311595, PubMed:24019530). Is able to hydrolyze the cell walls of several bacterial species (i.e. Paenibacillus sp., B.avium, E.coli DH5alpha, E.aerogenes, L.acidophilus, B.thuringiensis, B.pumilus, B.subtilis and E.coli W3110), thereby showing that it is a cell-wall hydrolase with broad-spectrum activity (PubMed:24311595). May have a role in peptidoglycan fragment recycling (PubMed:24019530).<ref>PMID:24019530</ref> <ref>PMID:24311595</ref>	+	[https://www.uniprot.org/uniprot/O69684_MYCTO O69684_MYCTO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Myctu~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~N-acetylmuramoyl-L-alanine amidase~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Alber, T]]	+	[[Category: Alber T]]
-	[[Category: Huizar~~, J P~~]]	+	[[Category: Huizar JP]]
-	[[Category: Mavrici, D]]	+	[[Category: Mavrici D]]
-	[[Category: Prigozhin~~, D M]]~~	+	[[Category: Prigozhin DM]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Vansell HJ]]
-	[[Category: Vansell~~, H J]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: National institute of allergy and infectious disease]]~~	+
-	~~[[Category: Niaid]]~~	+
-	~~[[Category: Tbsgc]]~~	+
-	~~[[Category: Zn binding~~]]	+

OCA at 05:42, 16 May 2018

OCA — Wed, 16 May 2018 05:42:41 GMT

←Older revision		Revision as of 05:42, 16 May 2018
Line 3:		Line 3:
	<StructureSection load='4m6g' size='340' side='right' caption='[[4m6g]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='4m6g' size='340' side='right' caption='[[4m6g]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4m6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M6G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4m6g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M6G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:~~ZINC+ION~~'>ZN</scene><~~/td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=ZGL:D-ALPHA-GLUTAMINE'>ZGL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~NonStdRes'><td class~~=~~"sblockLbl"><b>[[Non~~-~~Standard_Residue\|NonStd Res:]]</b~~></~~td><td class="sblockDat"~~><scene name='pdbligand=~~ZGL~~:~~D-ALPHA-GLUTAMINE~~'>~~ZGL~~</scene></td></tr>	+
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m6h\|4m6h]], [[4m6i\|4m6i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m6h\|4m6h]], [[4m6i\|4m6i]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">~~MT3820,~~ Rv3717 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv3717 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6g OCA], [http://pdbe.org/4m6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m6g RCSB], [http://www.ebi.ac.uk/pdbsum/4m6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6g OCA], [http://pdbe.org/4m6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m6g RCSB], [http://www.ebi.ac.uk/pdbsum/4m6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6g ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PEPAM_MYCTU PEPAM_MYCTU]] Cell-wall hydrolase that hydrolyzes the amide bond between N-acetylmuramic acid and L-alanine in cell-wall glycopeptides (PubMed:24311595, PubMed:24019530). Is able to hydrolyze the cell walls of several bacterial species (i.e. Paenibacillus sp., B.avium, E.coli DH5alpha, E.aerogenes, L.acidophilus, B.thuringiensis, B.pumilus, B.subtilis and E.coli W3110), thereby showing that it is a cell-wall hydrolase with broad-spectrum activity (PubMed:24311595). May have a role in peptidoglycan fragment recycling (PubMed:24019530).<ref>PMID:24019530</ref> <ref>PMID:24311595</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Myctu]]		[[Category: Myctu]]
		+	[[Category: N-acetylmuramoyl-L-alanine amidase]]
	[[Category: Alber, T]]		[[Category: Alber, T]]
	[[Category: Huizar, J P]]		[[Category: Huizar, J P]]

OCA at 14:16, 11 August 2016

OCA — Thu, 11 Aug 2016 14:16:29 GMT

←Older revision		Revision as of 14:16, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product==		==Structure of the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product==
	<StructureSection load='4m6g' size='340' side='right' caption='[[4m6g]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='4m6g' size='340' side='right' caption='[[4m6g]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4m6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_sp._h37rv Mycobacterium sp. h37rv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M6G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4m6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M6G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZGL:D-ALPHA-GLUTAMINE'>ZGL</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZGL:D-ALPHA-GLUTAMINE'>ZGL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m6h\|4m6h]], [[4m6i\|4m6i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m6h\|4m6h]], [[4m6i\|4m6i]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT3820, Rv3717 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 ~~Mycobacterium sp. H37Rv~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT3820, Rv3717 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m6g RCSB], [http://www.ebi.ac.uk/pdbsum/4m6g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6g OCA], [http://pdbe.org/4m6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m6g RCSB], [http://www.ebi.ac.uk/pdbsum/4m6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6g ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4m6g" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mycobacterium sp. h37rv~~]]	+	[[Category: Myctu]]
	[[Category: Alber, T]]		[[Category: Alber, T]]
	[[Category: Huizar, J P]]		[[Category: Huizar, J P]]

OCA at 08:56, 25 January 2015

OCA — Sun, 25 Jan 2015 08:56:38 GMT

←Older revision		Revision as of 08:56, 25 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4m6g\| PDB=4m6g \| SCENE= }}~~	+	==Structure of the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product==
-	===Structure of the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product===	+	<StructureSection load='4m6g' size='340' side='right' caption='[[4m6g]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24019530}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4m6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_sp._h37rv Mycobacterium sp. h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M6G FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZGL:D-ALPHA-GLUTAMINE'>ZGL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m6h\|4m6h]], [[4m6i\|4m6i]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT3820, Rv3717 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 Mycobacterium sp. H37Rv])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m6g RCSB], [http://www.ebi.ac.uk/pdbsum/4m6g PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Peptidoglycan hydrolases are key enzymes in bacterial cell wall homeostasis. Understanding the substrate specificity and biochemical activity of peptidoglycan hydrolases in Mycobacterium tuberculosis is of special interest as it can aid in the development of new cell wall targeting therapeutics. In this study, we report biochemical and structural characterization of the mycobacterial N-acetylmuramyl L-alanine amidase Rv3717. The crystal structure of Rv3717 in complex with a dipeptide product shows that, compared to previously characterized peptidoglycan amidases, the enzyme contains an extra disulfide-bonded beta-hairpin adjacent to the active site. The structure of two intermediates in assembly reveal that Zn2+ binding rearranges active site residues, and disulfide formation promotes folding of the beta-hairpin. Although Zn2+ is required for hydrolysis of muramyl dipeptide, disulfide oxidation is not required for activity on this substrate. The orientation of the product in the active site suggests a role for a conserved glutamate (E200) in catalysis; mutation of this residue abolishes activity. The product binds at the head of a closed tunnel, and the enzyme showed no activity on polymerized peptidoglycan. These results point to a potential role for Rv3717 in peptidoglycan fragment recycling.

-	~~==About this Structure==~~	+	Structural and biochemical analyses of Mycobacterium tuberculosis N-acetylmuramyl L-alanine amidase Rv3717 point to a role in peptidoglycan fragment recycling.,Prigozhin DM, Mavrici D, Huizar JP, Vansell HJ, Alber T J Biol Chem. 2013 Sep 9. PMID:24019530<ref>PMID:24019530</ref>
-	~~[[4m6g]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_sp~~.~~_h37rv Mycobacterium sp. h37rv]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024019530</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Mycobacterium sp. h37rv]]		[[Category: Mycobacterium sp. h37rv]]
-	[[Category: Alber, T.]]	+	[[Category: Alber, T]]
-	[[Category: Huizar, J P.]]	+	[[Category: Huizar, J P]]
-	[[Category: Mavrici, D.]]	+	[[Category: Mavrici, D]]
-	[[Category: Prigozhin, D M.]]	+	[[Category: Prigozhin, D M]]
-	[[Category: ~~TBSGC, TB~~ Structural ~~Genomics Consortium.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Vansell, H J.]]	+	[[Category: Vansell, H J]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: National institute of allergy and infectious disease]]		[[Category: National institute of allergy and infectious disease]]
	[[Category: Niaid]]		[[Category: Niaid]]
-	[[Category: Structural genomic]]
-	[[Category: Tb structural genomics consortium]]
	[[Category: Tbsgc]]		[[Category: Tbsgc]]
	[[Category: Zn binding]]		[[Category: Zn binding]]

OCA at 11:46, 20 November 2013

OCA — Wed, 20 Nov 2013 11:46:53 GMT

←Older revision		Revision as of 11:46, 20 November 2013
Line 4:		Line 4:

	==About this Structure==		==About this Structure==
-	[[4m6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis_h37rv~~ Mycobacterium ~~tuberculosis~~ h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA].	+	[[4m6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_sp._h37rv Mycobacterium sp. h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:024019530</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:024019530</ref><references group="xtra"/><references/>
-	[[Category: Mycobacterium ~~tuberculosis~~ h37rv]]	+	[[Category: Mycobacterium sp. h37rv]]
	[[Category: Alber, T.]]		[[Category: Alber, T.]]
	[[Category: Huizar, J P.]]		[[Category: Huizar, J P.]]

OCA at 06:49, 29 September 2013

OCA — Sun, 29 Sep 2013 06:49:21 GMT

←Older revision		Revision as of 06:49, 29 September 2013
Line 1:		Line 1:
	{{STRUCTURE_4m6g\| PDB=4m6g \| SCENE= }}		{{STRUCTURE_4m6g\| PDB=4m6g \| SCENE= }}
	===Structure of the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product===		===Structure of the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product===
		+	{{ABSTRACT_PUBMED_24019530}}

	==About this Structure==		==About this Structure==
	[[4m6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA].		[[4m6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024019530</ref><references group="xtra"/><references/>
	[[Category: Mycobacterium tuberculosis h37rv]]		[[Category: Mycobacterium tuberculosis h37rv]]
	[[Category: Alber, T.]]		[[Category: Alber, T.]]

OCA at 04:23, 20 September 2013

OCA — Fri, 20 Sep 2013 04:23:29 GMT

←Older revision		Revision as of 04:23, 20 September 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4m6g\| PDB=4m6g \| SCENE= }}
		+	===Structure of the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product===

-	~~The entry~~ 4m6g is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4m6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6G OCA].
-	~~Authors~~: ~~Prigozhin, D~~.M.~~, Mavrici, D~~., Huizar, J.P., ~~Vansell, H.J~~., ~~Alber, T~~., TB Structural Genomics Consortium ~~(TBSGC)~~	+	[[Category: Mycobacterium tuberculosis h37rv]]
-		+	[[Category: Alber, T.]]
-	~~Description~~: ~~Structure~~ of ~~the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product~~	+	[[Category: Huizar, J P.]]
		+	[[Category: Mavrici, D.]]
		+	[[Category: Prigozhin, D M.]]
		+	[[Category: TBSGC, TB Structural Genomics Consortium.]]
		+	[[Category: Vansell, H J.]]
		+	[[Category: Hydrolase]]
		+	[[Category: National institute of allergy and infectious disease]]
		+	[[Category: Niaid]]
		+	[[Category: Structural genomic]]
		+	[[Category: Tb structural genomics consortium]]
		+	[[Category: Tbsgc]]
		+	[[Category: Zn binding]]

OCA: Protected "4m6g" [edit=sysop:move=sysop]

OCA — Wed, 21 Aug 2013 07:56:25 GMT

Protected "4m6g" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:56, 21 August 2013

OCA: New page: '''Unreleased structure''' The entry 4m6g is ON HOLD Authors: Prigozhin, D.M., Mavrici, D., Huizar, J.P., Vansell, H.J., Alber, T., TB Structural Genomics Consortium (TBSGC) Descriptio...

OCA — Wed, 21 Aug 2013 07:56:24 GMT

New page: '''Unreleased structure''' The entry 4m6g is ON HOLD Authors: Prigozhin, D.M., Mavrici, D., Huizar, J.P., Vansell, H.J., Alber, T., TB Structural Genomics Consortium (TBSGC) Descriptio...

New page

'''Unreleased structure'''

The entry 4m6g is ON HOLD

Authors: Prigozhin, D.M., Mavrici, D., Huizar, J.P., Vansell, H.J., Alber, T., TB Structural Genomics Consortium (TBSGC)

Description: Structure of the Mycobacterium tuberculosis peptidoglycan amidase Rv3717 in complex with L-Alanine-iso-D-Glutamine reaction product