4m6r - Revision history

OCA at 08:55, 20 March 2024

OCA — Wed, 20 Mar 2024 08:55:59 GMT

←Older revision		Revision as of 08:55, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4m6r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M6R FirstGlance]. <br>		<table><tr><td colspan='2'>[[4m6r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M6R FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6r OCA], [https://pdbe.org/4m6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m6r RCSB], [https://www.ebi.ac.uk/pdbsum/4m6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6r ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6r OCA], [https://pdbe.org/4m6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m6r RCSB], [https://www.ebi.ac.uk/pdbsum/4m6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6r ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/MTNB_HUMAN MTNB_HUMAN] Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.<ref>PMID:15262985</ref> <ref>PMID:23285211</ref> <ref>PMID:22837397</ref>		[https://www.uniprot.org/uniprot/MTNB_HUMAN MTNB_HUMAN] Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.<ref>PMID:15262985</ref> <ref>PMID:23285211</ref> <ref>PMID:22837397</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	APIP, Apaf-1 interacting protein, has been known to inhibit two main types of programmed cell death, apoptosis and pyroptosis, and was recently found to be associated with cancers and inflammatory diseases. Distinct from its inhibitory role in cell death, APIP was also shown to act as a 5-methylthioribulose-1-phosphate dehydratase, or MtnB, in the methionine salvage pathway. Here we report the structural and enzymatic characterization of human APIP as an MtnB enzyme with a Km of 9.32 muM and a Vmax of 1.39 mumol min(-1) mg(-1). The crystal structure was determined at 2.0-A resolution, revealing an overall fold similar to members of the zinc-dependent class II aldolase family. APIP/MtnB exists as a tetramer in solution and exhibits an assembly with C4 symmetry in the crystal lattice. The pocket-shaped active site is located at the end of a long cleft between two adjacent subunits. We propose an enzymatic reaction mechanism involving Glu139* as a catalytic acid/base, as supported by enzymatic assay, substrate-docking study, and sequence conservation analysis. We explored the relationship between two distinct functions of APIP/MtnB, cell death inhibition, and methionine salvage, by measuring the ability of enzymatic mutants to inhibit cell death, and determined that APIP/MtnB functions as a cell death inhibitor independently of its MtnB enzyme activity for apoptosis induced by either hypoxia or etoposide, but dependently for caspase-1-induced pyroptosis. Our results establish the structural and biochemical groundwork for future mechanistic studies of the role of APIP/MtnB in modulating cell death and inflammation and in the development of related diseases.
-
-	Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme.,Kang W, Hong SH, Lee HM, Kim NY, Lim YC, Le le TM, Lim B, Kim HC, Kim TY, Ashida H, Yokota A, Hah SS, Chun KH, Jung YK, Yang JK Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):E54-61. doi: 10.1073/pnas.1308768111., Epub 2013 Dec 23. PMID:24367089<ref>PMID:24367089</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4m6r" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:59, 21 December 2022

OCA — Wed, 21 Dec 2022 10:59:52 GMT

←Older revision		Revision as of 10:59, 21 December 2022
Line 1:		Line 1:

	==Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme==		==Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme==
-	<StructureSection load='4m6r' size='340' side='right' caption='[[4m6r]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4m6r' size='340' side='right'caption='[[4m6r]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4m6r]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6R OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4M6R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4m6r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M6R FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APIP, CGI-29 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6r OCA], [https://pdbe.org/4m6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m6r RCSB], [https://www.ebi.ac.uk/pdbsum/4m6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6r ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylthioribulose_1-phosphate_dehydratase Methylthioribulose 1-phosphate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.109 4.2.1.109] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4m6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6r OCA], [~~http~~://pdbe.org/4m6r PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4m6r RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4m6r PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6r ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MTNB_HUMAN MTNB_HUMAN]] Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.<ref>PMID:15262985</ref> <ref>PMID:23285211</ref> <ref>PMID:22837397</ref>	+	[https://www.uniprot.org/uniprot/MTNB_HUMAN MTNB_HUMAN] Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.<ref>PMID:15262985</ref> <ref>PMID:23285211</ref> <ref>PMID:22837397</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Methylthioribulose 1-phosphate dehydratase~~]]	+	[[Category: Large Structures]]
-	[[Category: Ashida, H]]	+	[[Category: Ashida H]]
-	[[Category: Chun~~, K H~~]]	+	[[Category: Chun KH]]
-	[[Category: Hah~~, S S~~]]	+	[[Category: Hah SS]]
-	[[Category: Hong~~, S H~~]]	+	[[Category: Hong SH]]
-	[[Category: Jung~~, Y K~~]]	+	[[Category: Jung YK]]
-	[[Category: Kang, W]]	+	[[Category: Kang W]]
-	[[Category: Kim~~, H C~~]]	+	[[Category: Kim HC]]
-	[[Category: Kim~~, N Y~~]]	+	[[Category: Kim NY]]
-	[[Category: Kim~~, T Y~~]]	+	[[Category: Kim TY]]
-	[[Category: Le~~, L T.M~~]]	+	[[Category: Le LTM]]
-	[[Category: Lee~~, H M~~]]	+	[[Category: Lee HM]]
-	[[Category: Lim, B]]	+	[[Category: Lim B]]
-	[[Category: Lim~~, Y C~~]]	+	[[Category: Lim YC]]
-	[[Category: Yang~~, J K~~]]	+	[[Category: Yang JK]]
-	[[Category: Yokota, A]]	+	[[Category: Yokota A]]
-	~~[[Category: Apip]]~~	+
-	~~[[Category: Class ii aldolase family]]~~	+
-	~~[[Category: Dehydratase]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Mtnb~~]]	+

OCA at 18:32, 5 August 2016

OCA — Fri, 05 Aug 2016 18:32:08 GMT

←Older revision		Revision as of 18:32, 5 August 2016
Line 1:		Line 1:
		+
	==Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme==		==Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme==
	<StructureSection load='4m6r' size='340' side='right' caption='[[4m6r]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4m6r' size='340' side='right' caption='[[4m6r]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APIP, CGI-29 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APIP, CGI-29 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylthioribulose_1-phosphate_dehydratase Methylthioribulose 1-phosphate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.109 4.2.1.109] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylthioribulose_1-phosphate_dehydratase Methylthioribulose 1-phosphate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.109 4.2.1.109] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m6r RCSB], [http://www.ebi.ac.uk/pdbsum/4m6r PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6r OCA], [http://pdbe.org/4m6r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m6r RCSB], [http://www.ebi.ac.uk/pdbsum/4m6r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6r ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4m6r" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:59, 25 December 2014

OCA — Thu, 25 Dec 2014 20:59:25 GMT

←Older revision		Revision as of 20:59, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m6r RCSB], [http://www.ebi.ac.uk/pdbsum/4m6r PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m6r RCSB], [http://www.ebi.ac.uk/pdbsum/4m6r PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MTNB_HUMAN MTNB_HUMAN]] Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.<ref>PMID:15262985</ref> <ref>PMID:23285211</ref> <ref>PMID:22837397</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 16:34, 21 December 2014

OCA — Sun, 21 Dec 2014 16:34:44 GMT

←Older revision		Revision as of 16:34, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4m6r\| PDB=4m6r \| SCENE= }}~~	+	==Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme==
-	===Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme===	+	<StructureSection load='4m6r' size='340' side='right' caption='[[4m6r]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24367089}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4m6r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M6R FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APIP, CGI-29 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylthioribulose_1-phosphate_dehydratase Methylthioribulose 1-phosphate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.109 4.2.1.109] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m6r RCSB], [http://www.ebi.ac.uk/pdbsum/4m6r PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	APIP, Apaf-1 interacting protein, has been known to inhibit two main types of programmed cell death, apoptosis and pyroptosis, and was recently found to be associated with cancers and inflammatory diseases. Distinct from its inhibitory role in cell death, APIP was also shown to act as a 5-methylthioribulose-1-phosphate dehydratase, or MtnB, in the methionine salvage pathway. Here we report the structural and enzymatic characterization of human APIP as an MtnB enzyme with a Km of 9.32 muM and a Vmax of 1.39 mumol min(-1) mg(-1). The crystal structure was determined at 2.0-A resolution, revealing an overall fold similar to members of the zinc-dependent class II aldolase family. APIP/MtnB exists as a tetramer in solution and exhibits an assembly with C4 symmetry in the crystal lattice. The pocket-shaped active site is located at the end of a long cleft between two adjacent subunits. We propose an enzymatic reaction mechanism involving Glu139* as a catalytic acid/base, as supported by enzymatic assay, substrate-docking study, and sequence conservation analysis. We explored the relationship between two distinct functions of APIP/MtnB, cell death inhibition, and methionine salvage, by measuring the ability of enzymatic mutants to inhibit cell death, and determined that APIP/MtnB functions as a cell death inhibitor independently of its MtnB enzyme activity for apoptosis induced by either hypoxia or etoposide, but dependently for caspase-1-induced pyroptosis. Our results establish the structural and biochemical groundwork for future mechanistic studies of the role of APIP/MtnB in modulating cell death and inflammation and in the development of related diseases.

-	~~==Function==~~	+	Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme.,Kang W, Hong SH, Lee HM, Kim NY, Lim YC, Le le TM, Lim B, Kim HC, Kim TY, Ashida H, Yokota A, Hah SS, Chun KH, Jung YK, Yang JK Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):E54-61. doi: 10.1073/pnas.1308768111., Epub 2013 Dec 23. PMID:24367089<ref>PMID:24367089</ref>
-	~~[[http://www.uniprot.org/uniprot/MTNB_HUMAN MTNB_HUMAN]] Catalyzes~~ the ~~dehydration~~ of ~~methylthioribulose-1-phosphate (MTRu-1-P) into 2~~,~~3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the~~ methionine salvage ~~pathway~~, ~~which plays a key role in cancer~~, ~~apoptosis~~, ~~microbial proliferation and inflammation~~. ~~May inhibit the CASP1-related inflammatory response~~ (~~pyroptosis~~)~~, the CASP9~~-~~dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death~~.~~<ref>PMID~~:~~15262985<~~/~~ref> <ref>~~PMID:~~23285211</ref>~~ <ref>PMID:~~22837397~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4m6r]] is~~ a ~~4 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4M6R OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:024367089</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
	[[Category: Methylthioribulose 1-phosphate dehydratase]]		[[Category: Methylthioribulose 1-phosphate dehydratase]]
-	[[Category: Ashida, H.]]	+	[[Category: Ashida, H]]
-	[[Category: Chun, K H.]]	+	[[Category: Chun, K H]]
-	[[Category: Hah, S S.]]	+	[[Category: Hah, S S]]
-	[[Category: Hong, S H.]]	+	[[Category: Hong, S H]]
-	[[Category: Jung, Y K.]]	+	[[Category: Jung, Y K]]
-	[[Category: Kang, W.]]	+	[[Category: Kang, W]]
-	[[Category: Kim, H C.]]	+	[[Category: Kim, H C]]
-	[[Category: Kim, N Y.]]	+	[[Category: Kim, N Y]]
-	[[Category: Kim, T Y.]]	+	[[Category: Kim, T Y]]
-	[[Category: Le, L T.M.]]	+	[[Category: Le, L T.M]]
-	[[Category: Lee, H M.]]	+	[[Category: Lee, H M]]
-	[[Category: Lim, B.]]	+	[[Category: Lim, B]]
-	[[Category: Lim, Y C.]]	+	[[Category: Lim, Y C]]
-	[[Category: Yang, J K.]]	+	[[Category: Yang, J K]]
-	[[Category: Yokota, A.]]	+	[[Category: Yokota, A]]
	[[Category: Apip]]		[[Category: Apip]]
	[[Category: Class ii aldolase family]]		[[Category: Class ii aldolase family]]

OCA at 07:41, 29 January 2014

OCA — Wed, 29 Jan 2014 07:41:15 GMT

←Older revision		Revision as of 07:41, 29 January 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4m6r\| PDB=4m6r \| SCENE= }}
		+	===Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme===
		+	{{ABSTRACT_PUBMED_24367089}}

-	~~The entry 4m6r is ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/MTNB_HUMAN MTNB_HUMAN]] Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.<ref>PMID:15262985</ref> <ref>PMID:23285211</ref> <ref>PMID:22837397</ref>

-	~~Authors~~: ~~Kang, W~~.~~, Hong, S~~.~~H., Lee, H.M., Kim, N.Y., Lim, Y.C., Le, L.T.M., Kim, H.C., Kim, T.Y., Ashida, H., Yokota, A., Hah, S.S., Chun, K.H., Jung, Y~~.-~~K., Yang, J.K~~.	+	==About this Structure==
		+	[[4m6r]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6R OCA].

-	~~Description~~: ~~Structural and biochemical basis for the inhibition of cell death by APIP~~, ~~a methionine salvage enzyme~~	+	==Reference==
		+	<ref group="xtra">PMID:024367089</ref><references group="xtra"/><references/>
		+	[[Category: Methylthioribulose 1-phosphate dehydratase]]
		+	[[Category: Ashida, H.]]
		+	[[Category: Chun, K H.]]
		+	[[Category: Hah, S S.]]
		+	[[Category: Hong, S H.]]
		+	[[Category: Jung, Y K.]]
		+	[[Category: Kang, W.]]
		+	[[Category: Kim, H C.]]
		+	[[Category: Kim, N Y.]]
		+	[[Category: Kim, T Y.]]
		+	[[Category: Le, L T.M.]]
		+	[[Category: Lee, H M.]]
		+	[[Category: Lim, B.]]
		+	[[Category: Lim, Y C.]]
		+	[[Category: Yang, J K.]]
		+	[[Category: Yokota, A.]]
		+	[[Category: Apip]]
		+	[[Category: Class ii aldolase family]]
		+	[[Category: Dehydratase]]
		+	[[Category: Lyase]]
		+	[[Category: Mtnb]]

OCA at 06:46, 2 October 2013

OCA — Wed, 02 Oct 2013 06:46:42 GMT

←Older revision		Revision as of 06:46, 2 October 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4m6r is ON HOLD	+	The entry 4m6r is ON HOLD until Paper Publication

	Authors: Kang, W., Hong, S.H., Lee, H.M., Kim, N.Y., Lim, Y.C., Le, L.T.M., Kim, H.C., Kim, T.Y., Ashida, H., Yokota, A., Hah, S.S., Chun, K.H., Jung, Y.-K., Yang, J.K.		Authors: Kang, W., Hong, S.H., Lee, H.M., Kim, N.Y., Lim, Y.C., Le, L.T.M., Kim, H.C., Kim, T.Y., Ashida, H., Yokota, A., Hah, S.S., Chun, K.H., Jung, Y.-K., Yang, J.K.

	Description: Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme		Description: Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme

OCA: Protected "4m6r" [edit=sysop:move=sysop]

OCA — Wed, 21 Aug 2013 07:56:48 GMT

Protected "4m6r" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:56, 21 August 2013

OCA: New page: '''Unreleased structure''' The entry 4m6r is ON HOLD Authors: Kang, W., Hong, S.H., Lee, H.M., Kim, N.Y., Lim, Y.C., Le, L.T.M., Kim, H.C., Kim, T.Y., Ashida, H., Yokota, A., Hah, S.S.,...

OCA — Wed, 21 Aug 2013 07:56:47 GMT

New page: '''Unreleased structure''' The entry 4m6r is ON HOLD Authors: Kang, W., Hong, S.H., Lee, H.M., Kim, N.Y., Lim, Y.C., Le, L.T.M., Kim, H.C., Kim, T.Y., Ashida, H., Yokota, A., Hah, S.S.,...

New page

'''Unreleased structure'''

The entry 4m6r is ON HOLD

Authors: Kang, W., Hong, S.H., Lee, H.M., Kim, N.Y., Lim, Y.C., Le, L.T.M., Kim, H.C., Kim, T.Y., Ashida, H., Yokota, A., Hah, S.S., Chun, K.H., Jung, Y.-K., Yang, J.K.

Description: Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme