4m8j - Revision history

OCA at 16:34, 20 September 2023

OCA — Wed, 20 Sep 2023 16:34:10 GMT

←Older revision		Revision as of 16:34, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4m8j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_mirabilis_ATCC_29906 Proteus mirabilis ATCC 29906]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M8J FirstGlance]. <br>		<table><tr><td colspan='2'>[[4m8j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_mirabilis_ATCC_29906 Proteus mirabilis ATCC 29906]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M8J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.294Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8j OCA], [https://pdbe.org/4m8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m8j RCSB], [https://www.ebi.ac.uk/pdbsum/4m8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m8j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8j OCA], [https://pdbe.org/4m8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m8j RCSB], [https://www.ebi.ac.uk/pdbsum/4m8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m8j ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:02, 21 December 2022

OCA — Wed, 21 Dec 2022 11:02:26 GMT

←Older revision		Revision as of 11:02, 21 December 2022
Line 1:		Line 1:

	==Crystal structure of CaiT R262E bound to gamma-butyrobetaine==		==Crystal structure of CaiT R262E bound to gamma-butyrobetaine==
-	<StructureSection load='4m8j' size='340' side='right' caption='[[4m8j]], [[Resolution\|resolution]] 3.29Å' scene=''>	+	<StructureSection load='4m8j' size='340' side='right'caption='[[4m8j]], [[Resolution\|resolution]] 3.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4m8j]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Proteus_mirabilis_atcc_29906~~ Proteus mirabilis ~~atcc~~ 29906]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4M8J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4m8j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_mirabilis_ATCC_29906 Proteus mirabilis ATCC 29906]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M8J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">caiT, HMPREF0693_2800 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=525369 Proteus mirabilis ATCC 29906])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8j OCA], [https://pdbe.org/4m8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m8j RCSB], [https://www.ebi.ac.uk/pdbsum/4m8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m8j ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4m8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8j OCA], [~~http~~://pdbe.org/4m8j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4m8j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4m8j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4m8j ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~C2LLR0_PROMI C2LLR0_PROMI]~~] Catalyzes the exchange of L-carnitine for gamma-butyrobetaine ~~and related betaines (By similarity)~~.[HAMAP-Rule:MF_01049]	+	[https://www.uniprot.org/uniprot/CAIT_PROMH CAIT_PROMH] Catalyzes the exchange of L-carnitine for gamma-butyrobetaine.[HAMAP-Rule:MF_01049]<ref>PMID:20829798</ref> <ref>PMID:24101465</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Proteus mirabilis atcc 29906~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kalayil, S~~]]	+	[[Category: Proteus mirabilis ATCC 29906]]
-	[[Category: ~~Cait]]~~	+	[[Category: Kalayil S]]
-	~~[[Category: Carnitine/gamma-butyrobetaine antiporter]]~~	+
-	~~[[Category: Leut fold]]~~	+
-	~~[[Category: Plasma membrane]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 12:39, 4 August 2016

OCA — Thu, 04 Aug 2016 12:39:05 GMT

←Older revision		Revision as of 12:39, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of CaiT R262E bound to gamma-butyrobetaine==		==Crystal structure of CaiT R262E bound to gamma-butyrobetaine==
	<StructureSection load='4m8j' size='340' side='right' caption='[[4m8j]], [[Resolution\|resolution]] 3.29Å' scene=''>		<StructureSection load='4m8j' size='340' side='right' caption='[[4m8j]], [[Resolution\|resolution]] 3.29Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">caiT, HMPREF0693_2800 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=525369 Proteus mirabilis ATCC 29906])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">caiT, HMPREF0693_2800 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=525369 Proteus mirabilis ATCC 29906])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m8j RCSB], [http://www.ebi.ac.uk/pdbsum/4m8j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8j OCA], [http://pdbe.org/4m8j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m8j RCSB], [http://www.ebi.ac.uk/pdbsum/4m8j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m8j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4m8j" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:36, 24 December 2014

OCA — Wed, 24 Dec 2014 14:36:41 GMT

←Older revision		Revision as of 14:36, 24 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4m8j\| PDB=4m8j \| SCENE= }}~~	+	==Crystal structure of CaiT R262E bound to gamma-butyrobetaine==
-	===Crystal structure of CaiT R262E bound to gamma-butyrobetaine===	+	<StructureSection load='4m8j' size='340' side='right' caption='[[4m8j]], [[Resolution\|resolution]] 3.29Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24101465}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4m8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Proteus_mirabilis_atcc_29906 Proteus mirabilis atcc 29906]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M8J FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NM2:3-CARBOXY-N,N,N-TRIMETHYLPROPAN-1-AMINIUM'>NM2</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">caiT, HMPREF0693_2800 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=525369 Proteus mirabilis ATCC 29906])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m8j RCSB], [http://www.ebi.ac.uk/pdbsum/4m8j PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/C2LLR0_PROMI C2LLR0_PROMI]] Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines (By similarity).[HAMAP-Rule:MF_01049]		[[http://www.uniprot.org/uniprot/C2LLR0_PROMI C2LLR0_PROMI]] Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines (By similarity).[HAMAP-Rule:MF_01049]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Most secondary-active transporters transport their substrates using an electrochemical ion gradient. In contrast, the carnitine transporter (CaiT) is an ion-independent, l-carnitine/gamma-butyrobetaine antiporter belonging to the betaine/carnitine/choline transporter family of secondary transporters. Recently determined crystal structures of CaiT from Escherichia coli and Proteus mirabilis revealed an inverted five-transmembrane-helix repeat similar to that in the amino acid/Na+ symporter LeuT. The ion independence of CaiT makes it unique in this family. Here we show that mutations of arginine 262 (R262) make CaiT Na+-dependent. The transport activity of R262 mutants increased by 30-40% in the presence of a membrane potential, indicating substrate/Na+ cotransport. Structural and biochemical characterization revealed that R262 plays a crucial role in substrate binding by stabilizing the partly unwound TM1' helix. Modeling CaiT from P. mirabilis in the outward-open and closed states on the corresponding structures of the related symporter BetP reveals alternating orientations of the buried R262 sidechain, which mimic sodium binding and unbinding in the Na+-coupled substrate symporters. We propose that a similar mechanism is operative in other Na+/H+-independent transporters, in which a positively charged amino acid replaces the cotransported cation. The oscillation of the R262 sidechain in CaiT indicates how a positive charge triggers the change between outward-open and inward-open conformations as a unifying critical step in LeuT-type transporters.

-	~~==About this Structure==~~	+	Arginine oscillation explains Na+ independence in the substrate/product antiporter CaiT.,Kalayil S, Schulze S, Kuhlbrandt W Proc Natl Acad Sci U S A. 2013 Oct 7. PMID:24101465<ref>PMID:24101465</ref>
-	~~[[4m8j]] is a 1 chain structure with sequence from [http:~~/~~/en~~.~~wikipedia~~.~~org/wiki/Proteus_mirabilis_atcc_29906 Proteus mirabilis atcc 29906]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4M8J OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024101465</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Proteus mirabilis atcc 29906]]		[[Category: Proteus mirabilis atcc 29906]]
-	[[Category: Kalayil, S.]]	+	[[Category: Kalayil, S]]
	[[Category: Cait]]		[[Category: Cait]]
	[[Category: Carnitine/gamma-butyrobetaine antiporter]]		[[Category: Carnitine/gamma-butyrobetaine antiporter]]

OCA at 05:26, 23 October 2013

OCA — Wed, 23 Oct 2013 05:26:48 GMT

←Older revision		Revision as of 05:26, 23 October 2013
Line 1:		Line 1:
	{{STRUCTURE_4m8j\| PDB=4m8j \| SCENE= }}		{{STRUCTURE_4m8j\| PDB=4m8j \| SCENE= }}
	===Crystal structure of CaiT R262E bound to gamma-butyrobetaine===		===Crystal structure of CaiT R262E bound to gamma-butyrobetaine===
		+	{{ABSTRACT_PUBMED_24101465}}

	==Function==		==Function==
Line 7:		Line 8:
	==About this Structure==		==About this Structure==
	[[4m8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Proteus_mirabilis_atcc_29906 Proteus mirabilis atcc 29906]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8J OCA].		[[4m8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Proteus_mirabilis_atcc_29906 Proteus mirabilis atcc 29906]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8J OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024101465</ref><references group="xtra"/><references/>
	[[Category: Proteus mirabilis atcc 29906]]		[[Category: Proteus mirabilis atcc 29906]]
	[[Category: Kalayil, S.]]		[[Category: Kalayil, S.]]

OCA at 06:41, 10 October 2013

OCA — Thu, 10 Oct 2013 06:41:20 GMT

←Older revision		Revision as of 06:41, 10 October 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4m8j\| PDB=4m8j \| SCENE= }}
		+	===Crystal structure of CaiT R262E bound to gamma-butyrobetaine===

-	~~The entry 4m8j is ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/C2LLR0_PROMI C2LLR0_PROMI]] Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines (By similarity).[HAMAP-Rule:MF_01049]

-	~~Authors~~: Kalayil, S.	+	==About this Structure==
-		+	[[4m8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Proteus_mirabilis_atcc_29906 Proteus mirabilis atcc 29906]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M8J OCA].
-	~~Description~~: ~~Crystal structure of CaiT R262E bound to~~ gamma-butyrobetaine	+	[[Category: Proteus mirabilis atcc 29906]]
		+	[[Category: Kalayil, S.]]
		+	[[Category: Cait]]
		+	[[Category: Carnitine/gamma-butyrobetaine antiporter]]
		+	[[Category: Leut fold]]
		+	[[Category: Plasma membrane]]
		+	[[Category: Transport protein]]

OCA at 06:47, 2 October 2013

OCA — Wed, 02 Oct 2013 06:47:06 GMT

←Older revision		Revision as of 06:47, 2 October 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4m8j is ON HOLD	+	The entry 4m8j is ON HOLD until Paper Publication

	Authors: Kalayil, S.		Authors: Kalayil, S.

	Description: Crystal structure of CaiT R262E bound to gamma-butyrobetaine		Description: Crystal structure of CaiT R262E bound to gamma-butyrobetaine

OCA: Protected "4m8j" [edit=sysop:move=sysop]

OCA — Wed, 21 Aug 2013 07:58:54 GMT

Protected "4m8j" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:58, 21 August 2013

OCA: New page: '''Unreleased structure''' The entry 4m8j is ON HOLD Authors: Kalayil, S. Description: Crystal structure of CaiT R262E bound to gamma-butyrobetaine

OCA — Wed, 21 Aug 2013 07:58:53 GMT

New page: '''Unreleased structure''' The entry 4m8j is ON HOLD Authors: Kalayil, S. Description: Crystal structure of CaiT R262E bound to gamma-butyrobetaine

New page

'''Unreleased structure'''

The entry 4m8j is ON HOLD

Authors: Kalayil, S.

Description: Crystal structure of CaiT R262E bound to gamma-butyrobetaine