4mat - Revision history

OCA at 16:35, 20 September 2023

OCA — Wed, 20 Sep 2023 16:35:14 GMT

←Older revision		Revision as of 16:35, 20 September 2023
Line 3:		Line 3:
	<StructureSection load='4mat' size='340' side='right'caption='[[4mat]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4mat' size='340' side='right'caption='[[4mat]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4mat]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4MAT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4mat]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MAT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=NA:SODIUM+ION'>NA</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Methionyl_aminopeptidase Methionyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.18 3.4.11.18]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4mat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mat OCA], [~~http~~://pdbe.org/4mat PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4mat RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4mat PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4mat ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mat OCA], [https://pdbe.org/4mat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mat RCSB], [https://www.ebi.ac.uk/pdbsum/4mat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mat ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~AMPM_ECOLI AMPM_ECOLI]~~] Removes the N-terminal methionine from nascent proteins.	+	[https://www.uniprot.org/uniprot/MAP1_ECOLI MAP1_ECOLI] Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.[HAMAP-Rule:MF_01974]<ref>PMID:20521764</ref> <ref>PMID:3027045</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Methionyl aminopeptidase]]~~	+	[[Category: Lim S]]
-	[[Category: Lim, S]]	+	[[Category: Lowther WT]]
-	[[Category: Lowther~~, W T~~]]	+	[[Category: Madden DT]]
-	[[Category: Madden~~, D T~~]]	+	[[Category: Matthews BW]]
-	[[Category: Matthews~~, B W~~]]	+	[[Category: Orville AM]]
-	[[Category: Orville~~, A M~~]]	+	[[Category: Rich DH]]
-	[[Category: Rich~~, D H]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Site-directed mutant~~]]	+

OCA at 15:58, 20 November 2019

OCA — Wed, 20 Nov 2019 15:58:06 GMT

←Older revision		Revision as of 15:58, 20 November 2019
Line 1:		Line 1:

	==E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT==		==E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT==
-	<StructureSection load='4mat' size='340' side='right' caption='[[4mat]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4mat' size='340' side='right'caption='[[4mat]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mat]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MAT FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mat]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MAT FirstGlance]. <br>
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Aminopeptidase\|Aminopeptidase]]	+	*[[Aminopeptidase 3D structures\|Aminopeptidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Methionyl aminopeptidase]]		[[Category: Methionyl aminopeptidase]]
	[[Category: Lim, S]]		[[Category: Lim, S]]

OCA at 06:46, 14 March 2018

OCA — Wed, 14 Mar 2018 06:46:51 GMT

←Older revision		Revision as of 06:46, 14 March 2018
Line 1:		Line 1:
		+
	==E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT==		==E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT==
	<StructureSection load='4mat' size='340' side='right' caption='[[4mat]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4mat' size='340' side='right' caption='[[4mat]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionyl_aminopeptidase Methionyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.18 3.4.11.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionyl_aminopeptidase Methionyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.18 3.4.11.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mat OCA], [http://pdbe.org/4mat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mat RCSB], [http://www.ebi.ac.uk/pdbsum/4mat PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mat OCA], [http://pdbe.org/4mat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mat RCSB], [http://www.ebi.ac.uk/pdbsum/4mat PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mat ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ma/4mat_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ma/4mat_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:52, 8 February 2016

OCA — Mon, 08 Feb 2016 06:52:54 GMT

←Older revision		Revision as of 06:52, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='4mat' size='340' side='right' caption='[[4mat]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4mat' size='340' side='right' caption='[[4mat]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4mat]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MAT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4mat]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MAT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionyl_aminopeptidase Methionyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.18 3.4.11.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionyl_aminopeptidase Methionyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.18 3.4.11.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mat RCSB], [http://www.ebi.ac.uk/pdbsum/4mat PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mat OCA], [http://pdbe.org/4mat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mat RCSB], [http://www.ebi.ac.uk/pdbsum/4mat PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4mat ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4mat" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Methionyl aminopeptidase]]		[[Category: Methionyl aminopeptidase]]
	[[Category: Lim, S]]		[[Category: Lim, S]]

OCA at 21:27, 24 December 2014

OCA — Wed, 24 Dec 2014 21:27:19 GMT

←Older revision		Revision as of 21:27, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mat RCSB], [http://www.ebi.ac.uk/pdbsum/4mat PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mat RCSB], [http://www.ebi.ac.uk/pdbsum/4mat PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AMPM_ECOLI AMPM_ECOLI]] Removes the N-terminal methionine from nascent proteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 17:03, 10 December 2014

OCA — Wed, 10 Dec 2014 17:03:08 GMT

←Older revision		Revision as of 17:03, 10 December 2014
Line 1:		Line 1:
-	[[~~Image~~:4mat.~~png\|left~~\|200px]]	+	==E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT==
		+	<StructureSection load='4mat' size='340' side='right' caption='[[4mat]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4mat]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MAT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionyl_aminopeptidase Methionyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.18 3.4.11.18] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mat RCSB], [http://www.ebi.ac.uk/pdbsum/4mat PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ma/4mat_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	By improving the expression and purification of Escherichia coli methionine aminopeptidase (eMetAP) and using slightly different crystallization conditions, the resolution of the parent structure was extended from 2.4 to 1.9 A resolution. This has permitted visualization of the coordination geometry and solvent structure of the active-site dinuclear metal center. One solvent molecule (likely a mu-hydroxide) bridges the trigonal bipyramidal (Co1) and octahedral (Co2) cobalt ions. A second solvent (possibly a hydroxide ion) is bound terminally to Co2. A monovalent cation binding site was also identified about 13 A away from the metal center at an interface between the two subdomains of the protein. The first structure of a substrate-like inhibitor, (3R)-amino-(2S)-hydroxyheptanoyl-L-Ala-L-Leu-L-Val-L-Phe-OMe, bound to a methionine aminopeptidase, has also been determined. This inhibitor coordinates the metal center through four interactions as follows: (i) ligation of the N-terminal (3R)-nitrogen to Co2, (ii, iii) bridging coordination of the (2S)-hydroxyl group, and (iv) terminal ligation to Co1 by the keto oxygen of the pseudo-peptide linkage. Inhibitor binding occurs with the displacement of two solvent ligands and the expansion of the coordination sphere of Co1. In addition to the tetradentate, bis-chelate metal coordination, the substrate analogue forms hydrogen bonds with His79 and His178, two conserved residues within the active site of all MetAPs. To evaluate their importance in catalysis His79 and His178 were replaced with alanine. Both substitutions, but especially that of His79, reduce activity. The structure of the His79Ala apoenzyme and the comparison of its electronic absorption spectra with other variants suggest that the loss in activity is not due to a conformational change or a defective metal center. Two different reaction mechanisms are proposed and are compared to those of related enzymes. These results also suggest that inhibitors analogous to that reported here may be useful in preventing angiogenesis in cancer and in the treatment of microbial and fungal infections.

-	~~{{STRUCTURE_4mat\| PDB=4mat \| SCENE= }}~~	+	Escherichia coli methionine aminopeptidase: implications of crystallographic analyses of the native, mutant, and inhibited enzymes for the mechanism of catalysis.,Lowther WT, Orville AM, Madden DT, Lim S, Rich DH, Matthews BW Biochemistry. 1999 Jun 15;38(24):7678-88. PMID:10387007<ref>PMID:10387007</ref>

-	~~===E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10387007}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[4mat]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA].~~	+

	==See Also==		==See Also==
	*[[Aminopeptidase\|Aminopeptidase]]		*[[Aminopeptidase\|Aminopeptidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010387007<~~/~~ref~~><~~ref group="xtra">PMID:015211524</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Methionyl aminopeptidase]]		[[Category: Methionyl aminopeptidase]]
-	[[Category: Lim, S.]]	+	[[Category: Lim, S]]
-	[[Category: Lowther, W T.]]	+	[[Category: Lowther, W T]]
-	[[Category: Madden, D T.]]	+	[[Category: Madden, D T]]
-	[[Category: Matthews, B W.]]	+	[[Category: Matthews, B W]]
-	[[Category: Orville, A M.]]	+	[[Category: Orville, A M]]
-	[[Category: Rich, D H.]]	+	[[Category: Rich, D H]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Site-directed mutant]]		[[Category: Site-directed mutant]]

OCA at 09:18, 20 October 2012

OCA — Sat, 20 Oct 2012 09:18:23 GMT

←Older revision		Revision as of 09:18, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[4mat]] is a 1 chain structure ~~of [[Aminopeptidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA].	+	[[4mat]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA].

	==See Also==		==See Also==

OCA: Protected "4mat" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 22:23:54 GMT

Protected "4mat" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:23, 25 July 2012

OCA at 22:23, 25 July 2012

OCA — Wed, 25 Jul 2012 22:23:53 GMT

←Older revision		Revision as of 22:23, 25 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:4mat.png\|left\|200px]]		[[Image:4mat.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_4mat", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_4mat\| PDB=4mat \| SCENE= }}		{{STRUCTURE_4mat\| PDB=4mat \| SCENE= }}

	===E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT===		===E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10387007}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10387007 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_10387007}}		{{ABSTRACT_PUBMED_10387007}}

	==About this Structure==		==About this Structure==
-	~~4MAT~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA].	+	[[4mat]] is a 1 chain structure of [[Aminopeptidase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA].
		+
		+	==See Also==
		+	*[[Aminopeptidase\|Aminopeptidase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10387007~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010387007</ref><ref group="xtra">PMID:015211524</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Methionyl aminopeptidase]]		[[Category: Methionyl aminopeptidase]]
Line 32:		Line 23:
	[[Category: Orville, A M.]]		[[Category: Orville, A M.]]
	[[Category: Rich, D H.]]		[[Category: Rich, D H.]]
		+	[[Category: Hydrolase]]
	[[Category: Site-directed mutant]]		[[Category: Site-directed mutant]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 19:58:17 2009''

OCA at 17:58, 16 February 2009

OCA — Mon, 16 Feb 2009 17:58:19 GMT

←Older revision		Revision as of 17:58, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	4MAT is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA].	+	4MAT is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAT OCA].

	==Reference==		==Reference==
-	Escherichia coli methionine aminopeptidase: implications of crystallographic analyses of the native, mutant, and inhibited enzymes for the mechanism of catalysis., Lowther WT, Orville AM, Madden DT, Lim S, Rich DH, Matthews BW, Biochemistry. 1999 Jun 15;38(24):7678-88. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/10387007 10387007]~~	+	<ref group="xtra">PMID:10387007</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Methionyl aminopeptidase]]		[[Category: Methionyl aminopeptidase]]
-	[[Category: Single protein]]
	[[Category: Lim, S.]]		[[Category: Lim, S.]]
	[[Category: Lowther, W T.]]		[[Category: Lowther, W T.]]
Line 35:		Line 34:
	[[Category: Site-directed mutant]]		[[Category: Site-directed mutant]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Jul 3 13~~:43:~~51 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 19:58:17 2009''