4meg - Revision history

OCA at 16:36, 20 September 2023

OCA — Wed, 20 Sep 2023 16:36:28 GMT

←Older revision		Revision as of 16:36, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4meg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MEG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MEG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4meg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MEG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MEG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4meg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4meg OCA], [https://pdbe.org/4meg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4meg RCSB], [https://www.ebi.ac.uk/pdbsum/4meg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4meg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4meg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4meg OCA], [https://pdbe.org/4meg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4meg RCSB], [https://www.ebi.ac.uk/pdbsum/4meg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4meg ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:12, 21 December 2022

OCA — Wed, 21 Dec 2022 11:12:01 GMT

←Older revision		Revision as of 11:12, 21 December 2022
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	<StructureSection load='4meg' size='340' side='right'caption='[[4meg]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='4meg' size='340' side='right'caption='[[4meg]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4meg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MEG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4MEG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4meg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MEG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MEG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4meh\|4meh]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4meg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4meg OCA], [https://pdbe.org/4meg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4meg RCSB], [https://www.ebi.ac.uk/pdbsum/4meg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4meg ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4meg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4meg OCA], [~~http~~://pdbe.org/4meg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4meg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4meg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4meg ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
-	*[[Ribozyme\|Ribozyme]]	+	*[[Ribozyme 3D structures\|Ribozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Amare, A R.~~Ferre-D]]	+	[[Category: Ferre-D'Amare AR]]
-	[[Category: Lau~~, M W.L]]~~	+	[[Category: Lau MWL]]
-	~~[[Category: Glms ribozyme fold]]~~	+
-	~~[[Category: Metalloribozyme]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Self-cleavage]]~~	+
-	~~[[Category: Transesterification~~]]	+

OCA at 09:00, 10 April 2019

OCA — Wed, 10 Apr 2019 09:00:52 GMT

←Older revision		Revision as of 09:00, 10 April 2019
Line 1:		Line 1:

	==In vitro evolved glmS ribozyme triple mutant, magnesium ion complex==		==In vitro evolved glmS ribozyme triple mutant, magnesium ion complex==
-	<StructureSection load='4meg' size='340' side='right' caption='[[4meg]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<StructureSection load='4meg' size='340' side='right'caption='[[4meg]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4meg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MEG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MEG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4meg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MEG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MEG FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Amare, A R.Ferre-D]]		[[Category: Amare, A R.Ferre-D]]
	[[Category: Lau, M W.L]]		[[Category: Lau, M W.L]]

OCA at 20:21, 11 August 2016

OCA — Thu, 11 Aug 2016 20:21:04 GMT

←Older revision		Revision as of 20:21, 11 August 2016
Line 1:		Line 1:
		+
	==In vitro evolved glmS ribozyme triple mutant, magnesium ion complex==		==In vitro evolved glmS ribozyme triple mutant, magnesium ion complex==
	<StructureSection load='4meg' size='340' side='right' caption='[[4meg]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='4meg' size='340' side='right' caption='[[4meg]], [[Resolution\|resolution]] 3.10Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4meh\|4meh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4meh\|4meh]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4meg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4meg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4meg RCSB], [http://www.ebi.ac.uk/pdbsum/4meg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4meg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4meg OCA], [http://pdbe.org/4meg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4meg RCSB], [http://www.ebi.ac.uk/pdbsum/4meg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4meg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4meg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 13:08, 5 January 2015

OCA — Mon, 05 Jan 2015 13:08:51 GMT

←Older revision		Revision as of 13:08, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4meg\| PDB=4meg \| SCENE= }}~~	+	==In vitro evolved glmS ribozyme triple mutant, magnesium ion complex==
-	===In vitro evolved glmS ribozyme triple mutant, magnesium ion complex===	+	<StructureSection load='4meg' size='340' side='right' caption='[[4meg]], [[Resolution\|resolution]] 3.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24096303}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4meg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MEG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MEG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4meh\|4meh]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4meg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4meg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4meg RCSB], [http://www.ebi.ac.uk/pdbsum/4meg PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Uniquely among known ribozymes, the glmS ribozyme-riboswitch requires a small-molecule coenzyme, glucosamine-6-phosphate (GlcN6P). Although consistent with its gene-regulatory function, the use of GlcN6P is unexpected because all of the other characterized self-cleaving ribozymes use RNA functional groups or divalent cations for catalysis. To determine what active site features make this ribozyme reliant on GlcN6P and to evaluate whether it might have evolved from a coenzyme-independent ancestor, we isolated a GlcN6P-independent variant through in vitro selection. Three active site mutations suffice to generate a highly reactive RNA that adopts the wild-type fold but uses divalent cations for catalysis and is insensitive to GlcN6P. Biochemical and crystallographic comparisons of wild-type and mutant ribozymes show that a handful of functional groups fine-tune the RNA to be either coenzyme or cation dependent. These results indicate that a few mutations can confer new biochemical activities on structured RNAs. Thus, families of structurally related ribozymes with divergent function may exist.

-	~~==About this Structure==~~	+	An in vitro evolved glmS ribozyme has the wild-type fold but loses coenzyme dependence.,Lau MW, Ferre-D'Amare AR Nat Chem Biol. 2013 Oct 6. doi: 10.1038/nchembio.1360. PMID:24096303<ref>PMID:24096303</ref>
-	~~[[4meg]] is a 2 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4MEG OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:024096303</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
-	[[Category: Amare, A R.Ferre-D.]]	+
-	[[Category: Lau, M W.L.]]	+	==See Also==
		+	*[[Ribozyme\|Ribozyme]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Amare, A R.Ferre-D]]
		+	[[Category: Lau, M W.L]]
	[[Category: Glms ribozyme fold]]		[[Category: Glms ribozyme fold]]
	[[Category: Metalloribozyme]]		[[Category: Metalloribozyme]]

OCA at 08:03, 16 October 2013

OCA — Wed, 16 Oct 2013 08:03:20 GMT

←Older revision		Revision as of 08:03, 16 October 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4meg\| PDB=4meg \| SCENE= }}
		+	===In vitro evolved glmS ribozyme triple mutant, magnesium ion complex===
		+	{{ABSTRACT_PUBMED_24096303}}

-	~~The entry~~ 4meg is ~~ON HOLD~~	+	==About this Structure==
		+	[[4meg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MEG OCA].

-	~~Authors~~: ~~Lau~~, M.~~W.L.,~~ Ferre-D~~'Amare~~, A.R.	+	==Reference==
-		+	<ref group="xtra">PMID:024096303</ref><references group="xtra"/><references/>
-	~~Description~~: ~~In vitro evolved glmS~~ ribozyme ~~triple mutant, magnesium ion complex~~	+	[[Category: Amare, A R.Ferre-D.]]
		+	[[Category: Lau, M W.L.]]
		+	[[Category: Glms ribozyme fold]]
		+	[[Category: Metalloribozyme]]
		+	[[Category: Rna]]
		+	[[Category: Self-cleavage]]
		+	[[Category: Transesterification]]

OCA at 06:48, 2 October 2013

OCA — Wed, 02 Oct 2013 06:48:31 GMT

←Older revision		Revision as of 06:48, 2 October 2013
Line 3:		Line 3:
	The entry 4meg is ON HOLD		The entry 4meg is ON HOLD

-	Authors: Lau, M.WL., Ferre-D'Amare, A.R.	+	Authors: Lau, M.W.L., Ferre-D'Amare, A.R.

	Description: In vitro evolved glmS ribozyme triple mutant, magnesium ion complex		Description: In vitro evolved glmS ribozyme triple mutant, magnesium ion complex

OCA: Protected "4meg" [edit=sysop:move=sysop]

OCA — Wed, 04 Sep 2013 10:03:25 GMT

Protected "4meg" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:03, 4 September 2013

OCA: New page: '''Unreleased structure''' The entry 4meg is ON HOLD Authors: Lau, M.WL., Ferre-D'Amare, A.R. Description: In vitro evolved glmS ribozyme triple mutant, magnesium ion complex

OCA — Wed, 04 Sep 2013 10:03:24 GMT

New page: '''Unreleased structure''' The entry 4meg is ON HOLD Authors: Lau, M.WL., Ferre-D'Amare, A.R. Description: In vitro evolved glmS ribozyme triple mutant, magnesium ion complex

New page

'''Unreleased structure'''

The entry 4meg is ON HOLD

Authors: Lau, M.WL., Ferre-D'Amare, A.R.

Description: In vitro evolved glmS ribozyme triple mutant, magnesium ion complex