4mix - Revision history

OCA at 09:40, 28 December 2022

OCA — Wed, 28 Dec 2022 09:40:34 GMT

←Older revision		Revision as of 09:40, 28 December 2022
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	==PaToxG Glycosyltransferase==		==PaToxG Glycosyltransferase==
-	<StructureSection load='4mix' size='340' side='right' caption='[[4mix]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='4mix' size='340' side='right'caption='[[4mix]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4mix]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Phoaa Phoaa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4MIX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4mix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_asymbiotica_subsp._asymbiotica_ATCC_43949 Photorhabdus asymbiotica subsp. asymbiotica ATCC 43949]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MIX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=~~UD1~~:~~URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE~~'>~~UD1~~</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mix OCA], [https://pdbe.org/4mix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mix RCSB], [https://www.ebi.ac.uk/pdbsum/4mix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mix ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAU_02230 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=553480 PHOAA])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4mix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mix OCA], [~~http~~://pdbe.org/4mix PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4mix RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4mix PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4mix ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PATOX_PHOAA PATOX_PHOAA] Toxin that acts on host cells by modifying Rho proteins by tyrosine GlcNAcylation and heterotrimeric G alpha proteins by deamidation. Catalyzes the mono-O-GlcNAcylation of small GTPases of the Rho family (RhoA, RhoB, RhoC, Rac1, Rac2, Rac3, Cdc42) in eukaryotic host cells at the conserved tyrosine residue located in the switch I region (Tyr-32/34), using UDP-N-acetylglucosamine (UDP-GlcNAc) as the sugar donor; other GTPases of the Rho, Ras or Rab families are not substrates. Tyrosine glycosylation inhibits Rho activation and prevents interaction with downstream effectors, resulting in actin disassembly, inhibition of phagocytosis, cell rounding, and toxicity toward insects and mammalian cells. Also catalyzes the deamidation of the catalytic glutamine in heterotrimeric G alpha proteins (Gi, Gq/11), which blocks GTP hydrolysis and arrests the G proteins in a permanent active state leading to activation of Rho GTPases. Thus, PaTox hijacks host GTPase signaling in a bidirectional manner by deamidation-induced activation and glycosylation-induced inactivation of GTPases.<ref>PMID:24141704</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Phoaa~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Bogdanovic, X~~]]	+	[[Category: Photorhabdus asymbiotica subsp. asymbiotica ATCC 43949]]
-	[[Category: ~~Hunte, C~~]]	+	[[Category: Bogdanovic X]]
-	[[Category: ~~Wirth,~~ C]]	+	[[Category: Hunte C]]
-	[[Category: ~~Glycosyltransferase]]~~	+	[[Category: Wirth C]]
-	~~[[Category: Nucleotide-binding domain a/b/a]]~~	+
-	~~[[Category: Rho-protein]]~~	+
-	~~[[Category: Rossmann-like]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Tyrosine glycosylation]]~~	+
-	~~[[Category: Udp-glcnac~~]]	+

OCA at 18:30, 5 August 2016

OCA — Fri, 05 Aug 2016 18:30:31 GMT

←Older revision		Revision as of 18:30, 5 August 2016
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		+
	==PaToxG Glycosyltransferase==		==PaToxG Glycosyltransferase==
	<StructureSection load='4mix' size='340' side='right' caption='[[4mix]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4mix' size='340' side='right' caption='[[4mix]], [[Resolution\|resolution]] 1.80Å' scene=''>
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	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAU_02230 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=553480 PHOAA])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAU_02230 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=553480 PHOAA])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mix RCSB], [http://www.ebi.ac.uk/pdbsum/4mix PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mix OCA], [http://pdbe.org/4mix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mix RCSB], [http://www.ebi.ac.uk/pdbsum/4mix PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mix ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4mix" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:38, 21 December 2014

OCA — Sun, 21 Dec 2014 14:38:24 GMT

←Older revision		Revision as of 14:38, 21 December 2014
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-	~~{{STRUCTURE_4mix~~\| ~~PDB~~=4mix \| ~~SCENE~~= }}	+	==PaToxG Glycosyltransferase==
-	===~~PaToxG Glycosyltransferase~~===	+	<StructureSection load='4mix' size='340' side='right' caption='[[4mix]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24141704}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4mix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Phoaa Phoaa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MIX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAU_02230 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=553480 PHOAA])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mix RCSB], [http://www.ebi.ac.uk/pdbsum/4mix PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Entomopathogenic Photorhabdus asymbiotica is an emerging pathogen in humans. Here, we identified a P. asymbiotica protein toxin (PaTox), which contains a glycosyltransferase and a deamidase domain. PaTox mono-O-glycosylates Y32 (or Y34) of eukaryotic Rho GTPases by using UDP-N-acetylglucosamine (UDP-GlcNAc). Tyrosine glycosylation inhibits Rho activation and prevents interaction with downstream effectors, resulting in actin disassembly, inhibition of phagocytosis and toxicity toward insects and mammalian cells. The crystal structure of the PaTox glycosyltransferase domain in complex with UDP-GlcNAc determined at 1.8-A resolution represents a canonical GT-A fold and is the smallest glycosyltransferase toxin known. 1H-NMR analysis identifies PaTox as a retaining glycosyltransferase. The glutamine-deamidase domain of PaTox blocks GTP hydrolysis of heterotrimeric Galphaq/11 and Galphai proteins, thereby activating RhoA. Thus, PaTox hijacks host GTPase signaling in a bidirectional manner by deamidation-induced activation and glycosylation-induced inactivation of GTPases.

-	~~==About this Structure==~~	+	A bacterial toxin catalyzing tyrosine glycosylation of Rho and deamidation of G and G proteins.,Jank T, Bogdanovic X, Wirth C, Haaf E, Spoerner M, Bohmer KE, Steinemann M, Orth JH, Kalbitzer HR, Warscheid B, Hunte C, Aktories K Nat Struct Mol Biol. 2013 Oct 20. doi: 10.1038/nsmb.2688. PMID:24141704<ref>PMID:24141704</ref>
-	~~[[4mix]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Phoaa Phoaa]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4MIX OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024141704</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Phoaa]]		[[Category: Phoaa]]
-	[[Category: Bogdanovic, X.]]	+	[[Category: Bogdanovic, X]]
-	[[Category: Hunte, C.]]	+	[[Category: Hunte, C]]
-	[[Category: Wirth, C.]]	+	[[Category: Wirth, C]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Nucleotide-binding domain a/b/a]]		[[Category: Nucleotide-binding domain a/b/a]]

OCA at 11:57, 20 November 2013

OCA — Wed, 20 Nov 2013 11:57:00 GMT

←Older revision		Revision as of 11:57, 20 November 2013
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	==About this Structure==		==About this Structure==
-	[[4mix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Xenopus_itombwensis Xenopus itombwensis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIX OCA].	+	[[4mix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Phoaa Phoaa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIX OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:024141704</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:024141704</ref><references group="xtra"/><references/>
-	[[Category: ~~Xenopus itombwensis~~]]	+	[[Category: Phoaa]]
	[[Category: Bogdanovic, X.]]		[[Category: Bogdanovic, X.]]
	[[Category: Hunte, C.]]		[[Category: Hunte, C.]]

OCA at 06:26, 6 November 2013

OCA — Wed, 06 Nov 2013 06:26:47 GMT

←Older revision		Revision as of 06:26, 6 November 2013
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	{{STRUCTURE_4mix\| PDB=4mix \| SCENE= }}		{{STRUCTURE_4mix\| PDB=4mix \| SCENE= }}
	===PaToxG Glycosyltransferase===		===PaToxG Glycosyltransferase===
		+	{{ABSTRACT_PUBMED_24141704}}

	==About this Structure==		==About this Structure==
	[[4mix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Xenopus_itombwensis Xenopus itombwensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIX OCA].		[[4mix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Xenopus_itombwensis Xenopus itombwensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIX OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024141704</ref><references group="xtra"/><references/>
	[[Category: Xenopus itombwensis]]		[[Category: Xenopus itombwensis]]
	[[Category: Bogdanovic, X.]]		[[Category: Bogdanovic, X.]]

OCA at 08:11, 16 October 2013

OCA — Wed, 16 Oct 2013 08:11:32 GMT

←Older revision		Revision as of 08:11, 16 October 2013
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4mix\| PDB=4mix \| SCENE= }}
		+	===PaToxG Glycosyltransferase===

-	~~The entry~~ 4mix is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4mix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Xenopus_itombwensis Xenopus itombwensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MIX OCA].
-	~~Authors~~: Bogdanovic, X.~~, Wirth~~, C.~~, Hunte~~, C.	+	[[Category: Xenopus itombwensis]]
-		+	[[Category: Bogdanovic, X.]]
-	~~Description~~: ~~PaToxG~~ Glycosyltransferase	+	[[Category: Hunte, C.]]
		+	[[Category: Wirth, C.]]
		+	[[Category: Glycosyltransferase]]
		+	[[Category: Nucleotide-binding domain a/b/a]]
		+	[[Category: Rho-protein]]
		+	[[Category: Rossmann-like]]
		+	[[Category: Transferase]]
		+	[[Category: Tyrosine glycosylation]]
		+	[[Category: Udp-glcnac]]

OCA at 07:37, 29 September 2013

OCA — Sun, 29 Sep 2013 07:37:24 GMT

←Older revision		Revision as of 07:37, 29 September 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4mix is ON HOLD	+	The entry 4mix is ON HOLD until Paper Publication

	Authors: Bogdanovic, X., Wirth, C., Hunte, C.		Authors: Bogdanovic, X., Wirth, C., Hunte, C.

	Description: PaToxG Glycosyltransferase		Description: PaToxG Glycosyltransferase

OCA: Protected "4mix" [edit=sysop:move=sysop]

OCA — Thu, 12 Sep 2013 04:52:37 GMT

Protected "4mix" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:52, 12 September 2013

OCA: New page: '''Unreleased structure''' The entry 4mix is ON HOLD Authors: Bogdanovic, X., Wirth, C., Hunte, C. Description: PaToxG Glycosyltransferase

OCA — Thu, 12 Sep 2013 04:52:37 GMT

New page: '''Unreleased structure''' The entry 4mix is ON HOLD Authors: Bogdanovic, X., Wirth, C., Hunte, C. Description: PaToxG Glycosyltransferase

New page

'''Unreleased structure'''

The entry 4mix is ON HOLD

Authors: Bogdanovic, X., Wirth, C., Hunte, C.

Description: PaToxG Glycosyltransferase