4mja - Revision history

OCA at 16:38, 20 September 2023

OCA — Wed, 20 Sep 2023 16:38:24 GMT

←Older revision		Revision as of 16:38, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mja]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803_substr._Kazusa Synechocystis sp. PCC 6803 substr. Kazusa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MJA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mja]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803_substr._Kazusa Synechocystis sp. PCC 6803 substr. Kazusa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MJA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mja OCA], [https://pdbe.org/4mja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mja RCSB], [https://www.ebi.ac.uk/pdbsum/4mja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mja ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mja OCA], [https://pdbe.org/4mja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mja RCSB], [https://www.ebi.ac.uk/pdbsum/4mja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mja ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 09:41, 28 December 2022

OCA — Wed, 28 Dec 2022 09:41:01 GMT

←Older revision		Revision as of 09:41, 28 December 2022
Line 1:		Line 1:

	==Synechocystis sp. PCC 6803 glutaredoxin A-A75I==		==Synechocystis sp. PCC 6803 glutaredoxin A-A75I==
-	<StructureSection load='4mja' size='340' side='right' caption='[[4mja]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4mja' size='340' side='right'caption='[[4mja]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4mja]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Syny3 Syny3~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4MJA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4mja]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803_substr._Kazusa Synechocystis sp. PCC 6803 substr. Kazusa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MJA FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qmx\|3qmx]], [[4mjb\|4mjb]], [[4mjc\|4mjc]], [[4mje\|4mje]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mja OCA], [https://pdbe.org/4mja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mja RCSB], [https://www.ebi.ac.uk/pdbsum/4mja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mja ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ssr2061 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4mja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mja OCA], [~~http~~://pdbe.org/4mja PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4mja RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4mja PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4mja ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GLRX2_SYNY3 GLRX2_SYNY3]] Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins (By similarity).	+	[https://www.uniprot.org/uniprot/GLRX2_SYNY3 GLRX2_SYNY3] Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Syny3~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Knaff, D B~~]]	+	[[Category: Synechocystis sp. PCC 6803 substr. Kazusa]]
-	[[Category: ~~Sutton, R B~~]]	+	[[Category: Knaff DB]]
-	[[Category: ~~Electron transport]]~~	+	[[Category: Sutton RB]]
-	~~[[Category: Glutaredoxin]]~~	+
-	~~[[Category: Oxidation/reduction]]~~	+
-	~~[[Category: Thioredoxin fold~~]]	+

OCA at 19:56, 5 August 2016

OCA — Fri, 05 Aug 2016 19:56:20 GMT

←Older revision		Revision as of 19:56, 5 August 2016
Line 1:		Line 1:
		+
	==Synechocystis sp. PCC 6803 glutaredoxin A-A75I==		==Synechocystis sp. PCC 6803 glutaredoxin A-A75I==
	<StructureSection load='4mja' size='340' side='right' caption='[[4mja]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4mja' size='340' side='right' caption='[[4mja]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qmx\|3qmx]], [[4mjb\|4mjb]], [[4mjc\|4mjc]], [[4mje\|4mje]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qmx\|3qmx]], [[4mjb\|4mjb]], [[4mjc\|4mjc]], [[4mje\|4mje]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ssr2061 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ssr2061 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mja OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mja RCSB], [http://www.ebi.ac.uk/pdbsum/4mja PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mja OCA], [http://pdbe.org/4mja PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mja RCSB], [http://www.ebi.ac.uk/pdbsum/4mja PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mja ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4mja" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:24, 25 December 2014

OCA — Thu, 25 Dec 2014 05:24:19 GMT

←Older revision		Revision as of 05:24, 25 December 2014
Line 2:		Line 2:
	<StructureSection load='4mja' size='340' side='right' caption='[[4mja]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4mja' size='340' side='right' caption='[[4mja]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4mja]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]. <br>	+	<table><tr><td colspan='2'>[[4mja]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MJA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qmx\|3qmx]], [[4mjb\|4mjb]], [[4mjc\|4mjc]], [[4mje\|4mje]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qmx\|3qmx]], [[4mjb\|4mjb]], [[4mjc\|4mjc]], [[4mje\|4mje]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ssr2061 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ssr2061 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr>
-	<tr~~><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class~~='~~plainlinks~~'~~>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mja OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mja RCSB], [http://www.ebi.ac.uk/pdbsum/4mja PDBsum]</span></td></tr>
-	~~<tr~~><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mja OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mja RCSB], [http://www.ebi.ac.uk/pdbsum/4mja PDBsum]</span></td></tr>	+	</table>
-	<table>	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GLRX2_SYNY3 GLRX2_SYNY3]] Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 15:
	Utility of Synechocystis sp. PCC 6803 glutaredoxin A as a platform to study high-resolution mutagenesis of proteins.,Knaff DB, Sutton RB Front Plant Sci. 2013 Nov 15;4:461. doi: 10.3389/fpls.2013.00461. eCollection, 2013. PMID:24298277<ref>PMID:24298277</ref>		Utility of Synechocystis sp. PCC 6803 glutaredoxin A as a platform to study high-resolution mutagenesis of proteins.,Knaff DB, Sutton RB Front Plant Sci. 2013 Nov 15;4:461. doi: 10.3389/fpls.2013.00461. eCollection, 2013. PMID:24298277<ref>PMID:24298277</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Syny3]]		[[Category: Syny3]]
-	[[Category: Knaff, D B.]]	+	[[Category: Knaff, D B]]
-	[[Category: Sutton, R B.]]	+	[[Category: Sutton, R B]]
	[[Category: Electron transport]]		[[Category: Electron transport]]
	[[Category: Glutaredoxin]]		[[Category: Glutaredoxin]]
	[[Category: Oxidation/reduction]]		[[Category: Oxidation/reduction]]
	[[Category: Thioredoxin fold]]		[[Category: Thioredoxin fold]]

OCA at 09:40, 1 May 2014

OCA — Thu, 01 May 2014 09:40:31 GMT

←Older revision		Revision as of 09:40, 1 May 2014
Line 2:		Line 2:
	<StructureSection load='4mja' size='340' side='right' caption='[[4mja]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4mja' size='340' side='right' caption='[[4mja]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	[[4mja]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]. <br>	+	<table><tr><td colspan='2'>[[4mja]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]. <br>
-	<b>[[Related_structure\|Related:]]</b> [[3qmx\|3qmx]], [[4mjb\|4mjb]], [[4mjc\|4mjc]], [[4mje\|4mje]]<br>	+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qmx\|3qmx]], [[4mjb\|4mjb]], [[4mjc\|4mjc]], [[4mje\|4mje]]</td></tr>
-	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>	+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ssr2061 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr>
-	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mja OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mja RCSB], [http://www.ebi.ac.uk/pdbsum/4mja PDBsum]</span><br>	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mja OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mja RCSB], [http://www.ebi.ac.uk/pdbsum/4mja PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Glutaredoxin from the cyanobacterium Synechocystis sp. PCC 6803 is a small protein, containing only 88 amino acids, that participates in a large number of redox reactions, serving both as an electron donor for enzyme-catalyzed reductions and as a regulator of diverse metabolic pathways. The crystal structures of glutaredoxins from several species have been solved, including the glutaredoxin A isoform from the cyanobacterium Synechocystis sp. PCC 6803. We have utilized the small size of Synechocystis glutaredoxin A and its propensity to form protein crystals that diffract to high resolution to explore a long-standing question in biochemistry; i.e., what are the effects of mutations on protein structure and function? Taking advantage of these properties, we have initiated a long-term educational project that would examine the structural and biochemical changes in glutaredoxin as a function of single-point mutational replacements. Here, we report some of the mutational effects that we have observed to date.		Glutaredoxin from the cyanobacterium Synechocystis sp. PCC 6803 is a small protein, containing only 88 amino acids, that participates in a large number of redox reactions, serving both as an electron donor for enzyme-catalyzed reductions and as a regulator of diverse metabolic pathways. The crystal structures of glutaredoxins from several species have been solved, including the glutaredoxin A isoform from the cyanobacterium Synechocystis sp. PCC 6803. We have utilized the small size of Synechocystis glutaredoxin A and its propensity to form protein crystals that diffract to high resolution to explore a long-standing question in biochemistry; i.e., what are the effects of mutations on protein structure and function? Taking advantage of these properties, we have initiated a long-term educational project that would examine the structural and biochemical changes in glutaredoxin as a function of single-point mutational replacements. Here, we report some of the mutational effects that we have observed to date.
Line 12:		Line 15:

	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:14, 30 April 2014

OCA — Wed, 30 Apr 2014 10:14:21 GMT

←Older revision		Revision as of 10:14, 30 April 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	[[4mja]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]. <br>		[[4mja]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]. <br>
-	<b>Related:</b> [[3qmx\|3qmx]], [[4mjb\|4mjb]], [[4mjc\|4mjc]], [[4mje\|4mje]]<br>	+	<b>[[Related_structure\|Related:]]</b> [[3qmx\|3qmx]], [[4mjb\|4mjb]], [[4mjc\|4mjc]], [[4mje\|4mje]]<br>
	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>		<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mja OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mja RCSB], [http://www.ebi.ac.uk/pdbsum/4mja PDBsum]</span><br>
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Glutaredoxin from the cyanobacterium Synechocystis sp. PCC 6803 is a small protein, containing only 88 amino acids, that participates in a large number of redox reactions, serving both as an electron donor for enzyme-catalyzed reductions and as a regulator of diverse metabolic pathways. The crystal structures of glutaredoxins from several species have been solved, including the glutaredoxin A isoform from the cyanobacterium Synechocystis sp. PCC 6803. We have utilized the small size of Synechocystis glutaredoxin A and its propensity to form protein crystals that diffract to high resolution to explore a long-standing question in biochemistry; i.e., what are the effects of mutations on protein structure and function? Taking advantage of these properties, we have initiated a long-term educational project that would examine the structural and biochemical changes in glutaredoxin as a function of single-point mutational replacements. Here, we report some of the mutational effects that we have observed to date.		Glutaredoxin from the cyanobacterium Synechocystis sp. PCC 6803 is a small protein, containing only 88 amino acids, that participates in a large number of redox reactions, serving both as an electron donor for enzyme-catalyzed reductions and as a regulator of diverse metabolic pathways. The crystal structures of glutaredoxins from several species have been solved, including the glutaredoxin A isoform from the cyanobacterium Synechocystis sp. PCC 6803. We have utilized the small size of Synechocystis glutaredoxin A and its propensity to form protein crystals that diffract to high resolution to explore a long-standing question in biochemistry; i.e., what are the effects of mutations on protein structure and function? Taking advantage of these properties, we have initiated a long-term educational project that would examine the structural and biochemical changes in glutaredoxin as a function of single-point mutational replacements. Here, we report some of the mutational effects that we have observed to date.

OCA at 05:19, 30 April 2014

OCA — Wed, 30 Apr 2014 05:19:01 GMT

←Older revision		Revision as of 05:19, 30 April 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4mja~~\| ~~PDB~~=4mja \| ~~SCENE~~= }}	+	==Synechocystis sp. PCC 6803 glutaredoxin A-A75I==
-	===Synechocystis sp. PCC 6803 glutaredoxin A-~~A75I===~~	+	<StructureSection load='4mja' size='340' side='right' caption='[[4mja]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24298277}}~~	+	== Structural highlights ==
		+	[[4mja]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]. <br>
		+	<b>Related:</b> [[3qmx\|3qmx]], [[4mjb\|4mjb]], [[4mjc\|4mjc]], [[4mje\|4mje]]<br>
		+	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	== Publication Abstract from PubMed ==
		+	Glutaredoxin from the cyanobacterium Synechocystis sp. PCC 6803 is a small protein, containing only 88 amino acids, that participates in a large number of redox reactions, serving both as an electron donor for enzyme-catalyzed reductions and as a regulator of diverse metabolic pathways. The crystal structures of glutaredoxins from several species have been solved, including the glutaredoxin A isoform from the cyanobacterium Synechocystis sp. PCC 6803. We have utilized the small size of Synechocystis glutaredoxin A and its propensity to form protein crystals that diffract to high resolution to explore a long-standing question in biochemistry; i.e., what are the effects of mutations on protein structure and function? Taking advantage of these properties, we have initiated a long-term educational project that would examine the structural and biochemical changes in glutaredoxin as a function of single-point mutational replacements. Here, we report some of the mutational effects that we have observed to date.

-	~~==Function==~~	+	Utility of Synechocystis sp. PCC 6803 glutaredoxin A as a platform to study high-resolution mutagenesis of proteins.,Knaff DB, Sutton RB Front Plant Sci. 2013 Nov 15;4:461. doi: 10.3389/fpls.2013.00461. eCollection, 2013. PMID:24298277<ref>PMID:24298277</ref>
-	~~[[http://www~~.~~uniprot.org/uniprot/GLRX2_SYNY3 GLRX2_SYNY3]] Has~~ a ~~glutathione~~-~~disulfide oxidoreductase activity in the presence~~ of ~~NADPH and glutathione reductase. Reduces low molecular weight disulfides and~~ proteins ~~(By similarity)~~.	+

-	~~==About this Structure==~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4mja]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA]~~.	+	== References ==
-		+	<references/>
-	==~~Reference~~==	+	__TOC__
-	~~<ref group="xtra">PMID:024298277</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</StructureSection>
	[[Category: Syny3]]		[[Category: Syny3]]
	[[Category: Knaff, D B.]]		[[Category: Knaff, D B.]]

OCA at 08:48, 5 February 2014

OCA — Wed, 05 Feb 2014 08:48:40 GMT

←Older revision		Revision as of 08:48, 5 February 2014
Line 1:		Line 1:
	{{STRUCTURE_4mja\| PDB=4mja \| SCENE= }}		{{STRUCTURE_4mja\| PDB=4mja \| SCENE= }}
	===Synechocystis sp. PCC 6803 glutaredoxin A-A75I===		===Synechocystis sp. PCC 6803 glutaredoxin A-A75I===
		+	{{ABSTRACT_PUBMED_24298277}}

	==Function==		==Function==
Line 6:		Line 7:

	==About this Structure==		==About this Structure==
-	[[4mja]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA].	+	[[4mja]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024298277</ref><references group="xtra"/><references/>
		+	[[Category: Syny3]]
	[[Category: Knaff, D B.]]		[[Category: Knaff, D B.]]
	[[Category: Sutton, R B.]]		[[Category: Sutton, R B.]]

OCA at 07:33, 4 December 2013

OCA — Wed, 04 Dec 2013 07:33:24 GMT

←Older revision		Revision as of 07:33, 4 December 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4mja\| PDB=4mja \| SCENE= }}
		+	===Synechocystis sp. PCC 6803 glutaredoxin A-A75I===

-	~~The entry 4mja is ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/GLRX2_SYNY3 GLRX2_SYNY3]] Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins (By similarity).

-	~~Authors~~: ~~Sutton, R~~.B., Knaff, D.B.	+	==About this Structure==
-		+	[[4mja]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MJA OCA].
-	~~Description~~: ~~Synechocystis sp. PCC 6803 glutaredoxin A A75I~~	+	[[Category: Knaff, D B.]]
		+	[[Category: Sutton, R B.]]
		+	[[Category: Electron transport]]
		+	[[Category: Glutaredoxin]]
		+	[[Category: Oxidation/reduction]]
		+	[[Category: Thioredoxin fold]]

OCA: Protected "4mja" [edit=sysop:move=sysop]

OCA — Thu, 12 Sep 2013 04:53:05 GMT

Protected "4mja" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:53, 12 September 2013