4ml8 - Revision history

OCA at 09:46, 28 December 2022

OCA — Wed, 28 Dec 2022 09:46:57 GMT

←Older revision		Revision as of 09:46, 28 December 2022
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	<StructureSection load='4ml8' size='340' side='right'caption='[[4ml8]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4ml8' size='340' side='right'caption='[[4ml8]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ml8]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Maize Maize~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ML8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ML8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ml8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ML8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ML8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mla\|4mla]], [[1s1d\|1s1d]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ml8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ml8 OCA], [https://pdbe.org/4ml8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ml8 RCSB], [https://www.ebi.ac.uk/pdbsum/4ml8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ml8 ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cko2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ml8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ml8 OCA], [~~http~~://pdbe.org/4ml8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ml8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ml8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ml8 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q709Q5_MAIZE Q709Q5_MAIZE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Maize~~]]	+	[[Category: Zea mays]]
-	[[Category: Briozzo, P]]	+	[[Category: Briozzo P]]
-	[[Category: Koncitikova, R]]	+	[[Category: Koncitikova R]]
-	[[Category: Kopecny, D]]	+	[[Category: Kopecny D]]
-	[[Category: Morera, S]]	+	[[Category: Morera S]]
-	~~[[Category: Cytokinin binding]]~~	+
-	~~[[Category: Cytokinin oxidase/dehydrogenase]]~~	+
-	~~[[Category: Fad binding protein]]~~	+
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 08:49, 10 April 2019

OCA — Wed, 10 Apr 2019 08:49:06 GMT

←Older revision		Revision as of 08:49, 10 April 2019
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	==Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)==		==Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)==
-	<StructureSection load='4ml8' size='340' side='right' caption='[[4ml8]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='4ml8' size='340' side='right'caption='[[4ml8]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ml8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ML8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ML8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ml8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ML8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ML8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mla\|4mla]], [[1s1d\|1s1d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mla\|4mla]], [[1s1d\|1s1d]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ml8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ml8 OCA], [http://pdbe.org/4ml8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ml8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ml8 PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cko2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ml8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ml8 OCA], [http://pdbe.org/4ml8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ml8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ml8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ml8 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Maize]]
	[[Category: Briozzo, P]]		[[Category: Briozzo, P]]
	[[Category: Koncitikova, R]]		[[Category: Koncitikova, R]]

OCA at 19:07, 10 May 2016

OCA — Tue, 10 May 2016 19:07:37 GMT

←Older revision		Revision as of 19:07, 10 May 2016
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		+
	==Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)==		==Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)==
	<StructureSection load='4ml8' size='340' side='right' caption='[[4ml8]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4ml8' size='340' side='right' caption='[[4ml8]], [[Resolution\|resolution]] 2.70Å' scene=''>
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ml8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ml8 OCA], [http://pdbe.org/4ml8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ml8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ml8 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ml8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ml8 OCA], [http://pdbe.org/4ml8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ml8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ml8 PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytokinins are hormones that regulate plant development and their environmental responses. Their levels are mainly controlled by the cytokinin oxidase/dehydrogenase (CKO), which oxidatively cleaves cytokinins using redox-active electron acceptors. CKO belongs to the group of flavoproteins with an 8alpha-N1-histidyl FAD covalent linkage. Here, we investigated the role of seven active site residues: H105, D169, E288, V378, E381, P427 and L492, in substrate binding and catalysis of the CKO1 from maize (Zea mays, ZmCKO1) combining site-directed mutagenesis with kinetics and X-ray crystallography. Here, we identify E381 as a key residue for enzyme specificity that restricts substrate binding as well as quinone electron acceptor binding. We show that D169 is important for catalysis and that H105 covalently linked to FAD maintains the enzyme's structural integrity, stability and high rates with electron acceptors. The L492A mutation significantly modulates the cleavage of aromatic cytokinins and zeatin isomers. The high resolution X-ray structures of ZmCKO1 and the E381S variant in complex with N6-(2-isopentenyl)adenosine (iPR) reveal the binding mode of cytokinin ribosides. Those of ZmCKO2 and ZmCKO4a contain a mobile domain, which might contribute to binding of the N9 substituted cytokinins. This article is protected by copyright. All rights reserved.
		+
		+	Kinetic and structural investigation of the cytokinin oxidase/dehydrogenase active site.,Kopecny D, Koncitikova R, Popelka H, Briozzo P, Vigouroux A, Kopecna M, Zalabak D, Sebela M, Skopalova J, Frebort I, Morera S FEBS J. 2015 Oct 31. doi: 10.1111/febs.13581. PMID:26519657<ref>PMID:26519657</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4ml8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 20:57, 30 November 2015

OCA — Mon, 30 Nov 2015 20:57:09 GMT

←Older revision		Revision as of 20:57, 30 November 2015
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mla\|4mla]], [[1s1d\|1s1d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mla\|4mla]], [[1s1d\|1s1d]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ml8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ml8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ml8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ml8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ml8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ml8 OCA], [http://pdbe.org/4ml8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ml8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ml8 PDBsum]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 11:53, 11 March 2015

OCA — Wed, 11 Mar 2015 11:53:11 GMT

←Older revision		Revision as of 11:53, 11 March 2015
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-	'''~~Unreleased structure~~'''	+	==Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)==
-		+	<StructureSection load='4ml8' size='340' side='right' caption='[[4ml8]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~The entry~~ 4ml8 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4ml8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ML8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ML8 FirstGlance]. <br>
-	~~Authors~~: ~~Morera, S~~.~~, Kopecny, D~~.~~, Briozzo, P~~.~~, Koncitikova, R~~.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mla\|4mla]], [[1s1d\|1s1d]]</td></tr>
-	~~Description~~: ~~Structure of maize cytokinin oxidase~~/~~dehydrogenase 2~~ (~~ZmCKO2~~)	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ml8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ml8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ml8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ml8 PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Morera~~, S]]	+	__TOC__
		+	</StructureSection>
	[[Category: Briozzo, P]]		[[Category: Briozzo, P]]
-	[[Category: Kopecny, D]]
	[[Category: Koncitikova, R]]		[[Category: Koncitikova, R]]
		+	[[Category: Kopecny, D]]
		+	[[Category: Morera, S]]
		+	[[Category: Cytokinin binding]]
		+	[[Category: Cytokinin oxidase/dehydrogenase]]
		+	[[Category: Fad binding protein]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Oxidoreductase]]

OCA at 14:05, 24 December 2014

OCA — Wed, 24 Dec 2014 14:05:37 GMT

←Older revision		Revision as of 14:05, 24 December 2014
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	Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)		Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)
		+	[[Category: Unreleased Structures]]
		+	[[Category: Morera, S]]
		+	[[Category: Briozzo, P]]
		+	[[Category: Kopecny, D]]
		+	[[Category: Koncitikova, R]]

OCA at 06:50, 2 October 2013

OCA — Wed, 02 Oct 2013 06:50:17 GMT

←Older revision		Revision as of 06:50, 2 October 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ml8 is ON HOLD	+	The entry 4ml8 is ON HOLD until Paper Publication

	Authors: Morera, S., Kopecny, D., Briozzo, P., Koncitikova, R.		Authors: Morera, S., Kopecny, D., Briozzo, P., Koncitikova, R.

	Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)		Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)

OCA: Protected "4ml8" [edit=sysop:move=sysop]

OCA — Fri, 20 Sep 2013 03:40:37 GMT

Protected "4ml8" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:40, 20 September 2013

OCA: New page: '''Unreleased structure''' The entry 4ml8 is ON HOLD Authors: Morera, S., Kopecny, D., Briozzo, P., Koncitikova, R. Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (Z...

OCA — Fri, 20 Sep 2013 03:40:36 GMT

New page: '''Unreleased structure''' The entry 4ml8 is ON HOLD Authors: Morera, S., Kopecny, D., Briozzo, P., Koncitikova, R. Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (Z...

New page

'''Unreleased structure'''

The entry 4ml8 is ON HOLD

Authors: Morera, S., Kopecny, D., Briozzo, P., Koncitikova, R.

Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)