4mo4 - Revision history

OCA at 16:40, 20 September 2023

OCA — Wed, 20 Sep 2023 16:40:01 GMT

←Older revision		Revision as of 16:40, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mo4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MO4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mo4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MO4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.67Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mo4 OCA], [https://pdbe.org/4mo4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mo4 RCSB], [https://www.ebi.ac.uk/pdbsum/4mo4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mo4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mo4 OCA], [https://pdbe.org/4mo4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mo4 RCSB], [https://www.ebi.ac.uk/pdbsum/4mo4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mo4 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 09:57, 28 December 2022

OCA — Wed, 28 Dec 2022 09:57:04 GMT

←Older revision		Revision as of 09:57, 28 December 2022
Line 1:		Line 1:

	==Crystal structure of AnmK bound to AMPPCP==		==Crystal structure of AnmK bound to AMPPCP==
-	<StructureSection load='4mo4' size='340' side='right' caption='[[4mo4]], [[Resolution\|resolution]] 1.67Å' scene=''>	+	<StructureSection load='4mo4' size='340' side='right'caption='[[4mo4]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4mo4]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MO4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4MO4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4mo4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MO4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mo5\|4mo5]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mo4 OCA], [https://pdbe.org/4mo4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mo4 RCSB], [https://www.ebi.ac.uk/pdbsum/4mo4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mo4 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">anmK, PA0666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anhydro-N-acetylmuramic_acid_kinase Anhydro-N-acetylmuramic acid kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.170 2.7.1.170] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4mo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mo4 OCA], [~~http~~://pdbe.org/4mo4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4mo4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4mo4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4mo4 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ANMK_PSEAE ANMK_PSEAE]] Catalyzes the specific phosphorylation of 1,6-anhydro-N-acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling (By similarity).	+	[https://www.uniprot.org/uniprot/ANMK_PSEAE ANMK_PSEAE] Catalyzes the specific phosphorylation of 1,6-anhydro-N-acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Anhydro-N-acetylmuramic acid kinase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Bacik, J P~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Mark, B L~~]]	+	[[Category: Bacik JP]]
-	[[Category: ~~Atp-binding]]~~	+	[[Category: Mark BL]]
-	~~[[Category: Atpase domain]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 12:41, 5 August 2016

OCA — Fri, 05 Aug 2016 12:41:26 GMT

←Older revision		Revision as of 12:41, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of AnmK bound to AMPPCP==		==Crystal structure of AnmK bound to AMPPCP==
	<StructureSection load='4mo4' size='340' side='right' caption='[[4mo4]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='4mo4' size='340' side='right' caption='[[4mo4]], [[Resolution\|resolution]] 1.67Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">anmK, PA0666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">anmK, PA0666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anhydro-N-acetylmuramic_acid_kinase Anhydro-N-acetylmuramic acid kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.170 2.7.1.170] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anhydro-N-acetylmuramic_acid_kinase Anhydro-N-acetylmuramic acid kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.170 2.7.1.170] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mo4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mo4 RCSB], [http://www.ebi.ac.uk/pdbsum/4mo4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mo4 OCA], [http://pdbe.org/4mo4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mo4 RCSB], [http://www.ebi.ac.uk/pdbsum/4mo4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mo4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4mo4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:32, 25 December 2014

OCA — Thu, 25 Dec 2014 02:32:33 GMT

←Older revision		Revision as of 02:32, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mo4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MO4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MO4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mo4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MO4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MO4 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mo5\|4mo5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mo5\|4mo5]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">anmK, PA0666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">anmK, PA0666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anhydro-N-acetylmuramic_acid_kinase Anhydro-N-acetylmuramic acid kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.170 2.7.1.170] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anhydro-N-acetylmuramic_acid_kinase Anhydro-N-acetylmuramic acid kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.170 2.7.1.170] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mo4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mo4 RCSB], [http://www.ebi.ac.uk/pdbsum/4mo4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mo4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mo4 RCSB], [http://www.ebi.ac.uk/pdbsum/4mo4 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ANMK_PSEAE ANMK_PSEAE]] Catalyzes the specific phosphorylation of 1,6-anhydro-N-acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 17:
	Conformational itinerary of Pseudomonas aeruginosa 1,6-Anhydro-N-acetylmuramic acid kinase during its catalytic cycle.,Bacik JP, Tavassoli M, Patel TR, McKenna SA, Vocadlo DJ, Khajehpour M, Mark BL J Biol Chem. 2013 Dec 20. PMID:24362022<ref>PMID:24362022</ref>		Conformational itinerary of Pseudomonas aeruginosa 1,6-Anhydro-N-acetylmuramic acid kinase during its catalytic cycle.,Bacik JP, Tavassoli M, Patel TR, McKenna SA, Vocadlo DJ, Khajehpour M, Mark BL J Biol Chem. 2013 Dec 20. PMID:24362022<ref>PMID:24362022</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Anhydro-N-acetylmuramic acid kinase]]		[[Category: Anhydro-N-acetylmuramic acid kinase]]
-	[[Category: Bacik, J P.]]	+	[[Category: Bacik, J P]]
-	[[Category: Mark, B L.]]	+	[[Category: Mark, B L]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Atpase domain]]		[[Category: Atpase domain]]
	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 13:35, 18 May 2014

OCA — Sun, 18 May 2014 13:35:31 GMT

←Older revision		Revision as of 13:35, 18 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4mo4\| PDB~~=4mo4 \| ~~SCENE~~= }}	+	==Crystal structure of AnmK bound to AMPPCP==
-	===~~Crystal~~ structure of AnmK bound to AMPPCP~~===~~	+	<StructureSection load='4mo4' size='340' side='right' caption='[[4mo4]], [[Resolution\|resolution]] 1.67Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24362022}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4mo4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MO4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MO4 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mo5\|4mo5]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">anmK, PA0666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anhydro-N-acetylmuramic_acid_kinase Anhydro-N-acetylmuramic acid kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.170 2.7.1.170] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mo4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mo4 RCSB], [http://www.ebi.ac.uk/pdbsum/4mo4 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Anhydro-sugar kinases are unique from other sugar kinases in that they must cleave the 1,6-anhydro ring of their sugar substrate in order to phosphorylate it using ATP. Here we show that the peptidoglycan recycling enzyme 1,6-Anhydro-N-acetylmuramic acid kinase (AnmK) from Pseudomonas aeruginosa undergoes large conformational changes during its catalytic cycle, with its two domains rotating apart by up to 32 degrees around two hinge regions to expose an active site cleft into which the substrates 1,6-anhydroMurNAc and ATP can bind. X-ray structures of the open state bound to a nonhydrolyzable ATP analogue (AMPPCP) and 1,6-anhydroMurNAc provides detailed insight into a ternary complex that forms preceding an operative Michaelis complex. Structural analysis of the hinge regions demonstrates a role for nucleotide binding and possible cross-talk between the bound ligands to modulate the opening and closing of AnmK. Though AnmK was found to exhibit similar binding affinities for ATP, ADP and AMPPCP according to fluorescence spectroscopy, SAXS analyses revealed that AnmK adopts an open conformation in solution in the absence of ligand, and that it remains in this open state after binding AMPPCP, as we had observed for our crystal structure of this complex. In contrast, the enzyme favoured a closed conformation when bound to ADP in solution, consistent with a previous crystal structure of this complex. Together, our findings show that the open conformation of AnmK facilitates binding of both the sugar and nucleotide substrates, and that large structural rearrangements must occur upon closure of the enzyme to correctly align the substrates and residues of the enzyme for catalysis.

-	~~==Function==~~	+	Conformational itinerary of Pseudomonas aeruginosa 1,6-Anhydro-N-acetylmuramic acid kinase during its catalytic cycle.,Bacik JP, Tavassoli M, Patel TR, McKenna SA, Vocadlo DJ, Khajehpour M, Mark BL J Biol Chem. 2013 Dec 20. PMID:24362022<ref>PMID:24362022</ref>
-	~~[[http://www.uniprot.org/uniprot/ANMK_PSEAE ANMK_PSEAE]] Catalyzes the specific phosphorylation~~ of 1,6-~~anhydro~~-N-acetylmuramic acid ~~(anhMurNAc) with the simultaneous cleavage of the 1~~,~~6-anhydro ring~~, ~~generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein~~, ~~and thus plays a role in cell wall recycling (By similarity)~~.	+

-	~~==About this Structure==~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4mo4]] is~~ a ~~4 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4MO4 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:024362022</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Anhydro-N-acetylmuramic acid kinase]]		[[Category: Anhydro-N-acetylmuramic acid kinase]]
	[[Category: Bacik, J P.]]		[[Category: Bacik, J P.]]

OCA at 06:15, 22 January 2014

OCA — Wed, 22 Jan 2014 06:15:09 GMT

←Older revision		Revision as of 06:15, 22 January 2014
Line 1:		Line 1:
	{{STRUCTURE_4mo4\| PDB=4mo4 \| SCENE= }}		{{STRUCTURE_4mo4\| PDB=4mo4 \| SCENE= }}
	===Crystal structure of AnmK bound to AMPPCP===		===Crystal structure of AnmK bound to AMPPCP===
		+	{{ABSTRACT_PUBMED_24362022}}

	==Function==		==Function==
Line 7:		Line 8:
	==About this Structure==		==About this Structure==
	[[4mo4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MO4 OCA].		[[4mo4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MO4 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024362022</ref><references group="xtra"/><references/>
	[[Category: Anhydro-N-acetylmuramic acid kinase]]		[[Category: Anhydro-N-acetylmuramic acid kinase]]
	[[Category: Bacik, J P.]]		[[Category: Bacik, J P.]]

OCA at 12:14, 1 January 2014

OCA — Wed, 01 Jan 2014 12:14:54 GMT

←Older revision		Revision as of 12:14, 1 January 2014
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-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4mo4\| PDB=4mo4 \| SCENE= }}
		+	===Crystal structure of AnmK bound to AMPPCP===

-	~~The entry 4mo4 is ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/ANMK_PSEAE ANMK_PSEAE]] Catalyzes the specific phosphorylation of 1,6-anhydro-N-acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling (By similarity).

-	~~Authors~~: Bacik, J.P., Mark, B.L.	+	==About this Structure==
-		+	[[4mo4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MO4 OCA].
-	~~Description~~: ~~Crystal structure of AnmK bound to AMPPCP~~	+	[[Category: Anhydro-N-acetylmuramic acid kinase]]
		+	[[Category: Bacik, J P.]]
		+	[[Category: Mark, B L.]]
		+	[[Category: Atp-binding]]
		+	[[Category: Atpase domain]]
		+	[[Category: Kinase]]
		+	[[Category: Transferase]]

OCA at 06:43, 4 December 2013

OCA — Wed, 04 Dec 2013 06:43:55 GMT

←Older revision		Revision as of 06:43, 4 December 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4mo4 is ON HOLD	+	The entry 4mo4 is ON HOLD until Paper Publication

	Authors: Bacik, J.P., Mark, B.L.		Authors: Bacik, J.P., Mark, B.L.

	Description: Crystal structure of AnmK bound to AMPPCP		Description: Crystal structure of AnmK bound to AMPPCP

OCA: Protected "4mo4" [edit=sysop:move=sysop]

OCA — Fri, 20 Sep 2013 03:44:14 GMT

Protected "4mo4" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:44, 20 September 2013

OCA: New page: '''Unreleased structure''' The entry 4mo4 is ON HOLD Authors: Bacik, J.P., Mark, B.L. Description: Crystal structure of AnmK bound to AMPPCP

OCA — Fri, 20 Sep 2013 03:44:13 GMT

New page: '''Unreleased structure''' The entry 4mo4 is ON HOLD Authors: Bacik, J.P., Mark, B.L. Description: Crystal structure of AnmK bound to AMPPCP

New page

'''Unreleased structure'''

The entry 4mo4 is ON HOLD

Authors: Bacik, J.P., Mark, B.L.

Description: Crystal structure of AnmK bound to AMPPCP