4n13 - Revision history

OCA at 16:45, 20 September 2023

OCA — Wed, 20 Sep 2023 16:45:49 GMT

←Older revision		Revision as of 16:45, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4n13]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi_B31 Borreliella burgdorferi B31]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N13 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4n13]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi_B31 Borreliella burgdorferi B31]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N13 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n13 OCA], [https://pdbe.org/4n13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n13 RCSB], [https://www.ebi.ac.uk/pdbsum/4n13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n13 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n13 OCA], [https://pdbe.org/4n13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n13 RCSB], [https://www.ebi.ac.uk/pdbsum/4n13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n13 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:25, 28 December 2022

OCA — Wed, 28 Dec 2022 10:25:26 GMT

←Older revision		Revision as of 10:25, 28 December 2022
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	==Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi==		==Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi==
-	<StructureSection load='4n13' size='340' side='right' caption='[[4n13]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='4n13' size='340' side='right'caption='[[4n13]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n13]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Borbu Borbu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N13 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4N13 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4n13]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi_B31 Borreliella burgdorferi B31]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N13 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BB_0215 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224326 BORBU])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n13 OCA], [https://pdbe.org/4n13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n13 RCSB], [https://www.ebi.ac.uk/pdbsum/4n13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n13 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4n13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n13 OCA], [~~http~~://pdbe.org/4n13 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4n13 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4n13 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4n13 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PSTS_BORBU PSTS_BORBU] Binds inorganic phosphate with a Kd of 1.2 uM (PubMed:24318969). Part of the ABC transporter complex PstSACB involved in phosphate import (Probable).<ref>PMID:24318969</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[ABC transporter\|ABC transporter]]	+	*[[ABC transporter 3D structures\|ABC transporter 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Borbu~~]]	+	[[Category: Borreliella burgdorferi B31]]
-	[[Category: ~~Brautigam, C A~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Deka, R K~~]]	+	[[Category: Brautigam CA]]
-	[[Category: ~~Norgard, M V~~]]	+	[[Category: Deka RK]]
-	[[Category: ~~Abc transporter]]~~	+	[[Category: Norgard MV]]
-	~~[[Category: Class ii ligand-binding protein]]~~	+
-	~~[[Category: Ligand-binding protein]]~~	+
-	~~[[Category: Phosphate-binding protein]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 16:03, 4 August 2016

OCA — Thu, 04 Aug 2016 16:03:44 GMT

←Older revision		Revision as of 16:03, 4 August 2016
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		+
	==Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi==		==Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi==
	<StructureSection load='4n13' size='340' side='right' caption='[[4n13]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='4n13' size='340' side='right' caption='[[4n13]], [[Resolution\|resolution]] 1.30Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BB_0215 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224326 BORBU])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BB_0215 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224326 BORBU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n13 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n13 RCSB], [http://www.ebi.ac.uk/pdbsum/4n13 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n13 OCA], [http://pdbe.org/4n13 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n13 RCSB], [http://www.ebi.ac.uk/pdbsum/4n13 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n13 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4n13" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ABC transporter\|ABC transporter]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:17, 21 December 2014

OCA — Sun, 21 Dec 2014 18:17:00 GMT

←Older revision		Revision as of 18:17, 21 December 2014
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-	~~{{STRUCTURE_4n13\| PDB=4n13 \| SCENE= }}~~	+	==Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi==
-	===Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi===	+	<StructureSection load='4n13' size='340' side='right' caption='[[4n13]], [[Resolution\|resolution]] 1.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24318969}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4n13]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borbu Borbu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N13 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N13 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BB_0215 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224326 BORBU])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n13 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n13 RCSB], [http://www.ebi.ac.uk/pdbsum/4n13 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Lyme disease agent Borrelia burgdorferi, which is transmitted via a tick vector, is dependent on its tick and mammalian hosts for a number of essential nutrients. Like other bacterial diderms, it must transport these biochemicals from the extracellular milieu across two membranes, ultimately to the B. burgdorferi cytoplasm. In the current study, we established that a gene cluster comprising genes bb0215 through bb0218 is cotranscribed and is therefore an operon. Sequence analysis of these proteins suggested that they are the components of an ABC-type transporter responsible for translocating phosphate anions from the B. burgdorferi periplasm to the cytoplasm. Biophysical experiments established that the putative ligand-binding protein of this system, BbPstS (BB0215), binds to phosphate in solution. We determined the high-resolution (1.3 A) crystal structure of the protein in the absence of phosphate, revealing that the protein's fold is similar to other phosphate-binding proteins, and residues that are implicated in phosphate binding in other such proteins are conserved in BbPstS. Taken together, the gene products of bb0215-0218 function as a phosphate transporter for B. burgdorferi.

-	~~==About this Structure==~~	+	Sequence, biophysical, and structural analyses of the PstS lipoprotein (BB0215) from Borrelia burgdorferi reveal a likely binding component of an ABC-type phosphate transporter.,Brautigam CA, Ouyang Z, Deka RK, Norgard MV Protein Sci. 2013 Dec 8. doi: 10.1002/pro.2406. PMID:24318969<ref>PMID:24318969</ref>
-	~~[[4n13]] is~~ a ~~1 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4N13 OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024318969</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
-	[[Category: Brautigam, C A.]]	+	== References ==
-	[[Category: Deka, R K.]]	+	<references/>
-	[[Category: Norgard, M V.]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Borbu]]
		+	[[Category: Brautigam, C A]]
		+	[[Category: Deka, R K]]
		+	[[Category: Norgard, M V]]
	[[Category: Abc transporter]]		[[Category: Abc transporter]]
	[[Category: Class ii ligand-binding protein]]		[[Category: Class ii ligand-binding protein]]

OCA at 06:58, 22 January 2014

OCA — Wed, 22 Jan 2014 06:58:01 GMT

←Older revision		Revision as of 06:58, 22 January 2014
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4n13\| PDB=4n13 \| SCENE= }}
		+	===Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi===
		+	{{ABSTRACT_PUBMED_24318969}}

-	~~The entry~~ 4n13 is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4n13]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N13 OCA].

-	~~Authors~~: Brautigam, C.A., Deka, R.K., Norgard, M.V.	+	==Reference==
-		+	<ref group="xtra">PMID:024318969</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi~~	+	[[Category: Brautigam, C A.]]
		+	[[Category: Deka, R K.]]
		+	[[Category: Norgard, M V.]]
		+	[[Category: Abc transporter]]
		+	[[Category: Class ii ligand-binding protein]]
		+	[[Category: Ligand-binding protein]]
		+	[[Category: Phosphate-binding protein]]
		+	[[Category: Transport protein]]

OCA at 06:13, 23 October 2013

OCA — Wed, 23 Oct 2013 06:13:17 GMT

←Older revision		Revision as of 06:13, 23 October 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4n13 is ON HOLD	+	The entry 4n13 is ON HOLD until Paper Publication

	Authors: Brautigam, C.A., Deka, R.K., Norgard, M.V.		Authors: Brautigam, C.A., Deka, R.K., Norgard, M.V.

	Description: Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi		Description: Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi

OCA: Protected "4n13" [edit=sysop:move=sysop]

OCA — Thu, 10 Oct 2013 05:53:37 GMT

Protected "4n13" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:53, 10 October 2013

OCA: New page: '''Unreleased structure''' The entry 4n13 is ON HOLD Authors: Brautigam, C.A., Deka, R.K., Norgard, M.V. Description: Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi

OCA — Thu, 10 Oct 2013 05:53:36 GMT

New page: '''Unreleased structure''' The entry 4n13 is ON HOLD Authors: Brautigam, C.A., Deka, R.K., Norgard, M.V. Description: Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi

New page

'''Unreleased structure'''

The entry 4n13 is ON HOLD

Authors: Brautigam, C.A., Deka, R.K., Norgard, M.V.

Description: Crystal Structure of PstS (BB_0215) from Borrelia burgdorferi