4n23 - Revision history

OCA at 16:46, 20 September 2023

OCA — Wed, 20 Sep 2023 16:46:15 GMT

←Older revision		Revision as of 16:46, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4n23]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/CAS_virus CAS virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N23 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4n23]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/CAS_virus CAS virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N23 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n23 OCA], [https://pdbe.org/4n23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n23 RCSB], [https://www.ebi.ac.uk/pdbsum/4n23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n23 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n23 OCA], [https://pdbe.org/4n23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n23 RCSB], [https://www.ebi.ac.uk/pdbsum/4n23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n23 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:27, 28 December 2022

OCA — Wed, 28 Dec 2022 10:27:55 GMT

←Older revision		Revision as of 10:27, 28 December 2022
Line 1:		Line 1:

	==Crystal structure of the GP2 Core Domain from the California Academy of Science Virus, monoclinic symmetry==		==Crystal structure of the GP2 Core Domain from the California Academy of Science Virus, monoclinic symmetry==
-	<StructureSection load='4n23' size='340' side='right' caption='[[4n23]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4n23' size='340' side='right'caption='[[4n23]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n23]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cas_virus Cas~~ virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N23 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4N23 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4n23]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/CAS_virus CAS virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N23 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n21\|4n21]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n23 OCA], [https://pdbe.org/4n23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n23 RCSB], [https://www.ebi.ac.uk/pdbsum/4n23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n23 ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223561 CAS virus])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4n23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n23 OCA], [~~http~~://pdbe.org/4n23 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4n23 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4n23 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4n23 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/J7H5L9_9VIRU J7H5L9_9VIRU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4n23" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4n23" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glycoprotein GP 3D structures\|Glycoprotein GP 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cas~~ virus]]	+	[[Category: CAS virus]]
-	[[Category: ~~Almo, S C~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Dai, Z~~]]	+	[[Category: Almo SC]]
-	[[Category: ~~Koellhoffer, J F~~]]	+	[[Category: Dai Z]]
-	[[Category: ~~Lai, J R~~]]	+	[[Category: Koellhoffer JF]]
-	[[Category: ~~Malashkevich, V N~~]]	+	[[Category: Lai JR]]
-	[[Category: ~~Structural genomic~~]]	+	[[Category: Malashkevich VN]]
-	[[Category: Toro, R]]	+	[[Category: Toro R]]
-	~~[[Category: Marburg virus]]~~	+
-	~~[[Category: Nysgrc]]~~	+
-	~~[[Category: Post-fusion conformation]]~~	+
-	~~[[Category: Psi-biology]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 09:49, 11 August 2016

OCA — Thu, 11 Aug 2016 09:49:30 GMT

←Older revision		Revision as of 09:49, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the GP2 Core Domain from the California Academy of Science Virus, monoclinic symmetry==		==Crystal structure of the GP2 Core Domain from the California Academy of Science Virus, monoclinic symmetry==
	<StructureSection load='4n23' size='340' side='right' caption='[[4n23]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4n23' size='340' side='right' caption='[[4n23]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n21\|4n21]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n21\|4n21]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223561 CAS virus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223561 CAS virus])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n23 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n23 RCSB], [http://www.ebi.ac.uk/pdbsum/4n23 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n23 OCA], [http://pdbe.org/4n23 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n23 RCSB], [http://www.ebi.ac.uk/pdbsum/4n23 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n23 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4n23" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:09, 5 January 2015

OCA — Mon, 05 Jan 2015 09:09:11 GMT

←Older revision		Revision as of 09:09, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4n23\| PDB=4n23 \| SCENE= }}~~	+	==Crystal structure of the GP2 Core Domain from the California Academy of Science Virus, monoclinic symmetry==
-	===Crystal structure of the GP2 Core Domain from the California Academy of Science Virus, monoclinic symmetry===	+	<StructureSection load='4n23' size='340' side='right' caption='[[4n23]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24333483}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4n23]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Cas_virus Cas virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N23 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N23 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n21\|4n21]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223561 CAS virus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n23 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n23 RCSB], [http://www.ebi.ac.uk/pdbsum/4n23 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fusion of the viral and host cell membranes is a necessary first step for infection by enveloped viruses and is mediated by the envelope glycoprotein. The transmembrane subunits from the structurally defined "class I" glycoproteins adopt an alpha-helical "trimer-of-hairpins" conformation during the fusion pathway. Here, we present our studies on the envelope glycoprotein transmembrane subunit, GP2, of the CAS virus (CASV). CASV was recently identified from annulated tree boas (Corallus annulatus) with inclusion body disease and is implicated in the disease etiology. We have generated and characterized two protein constructs consisting of the predicted CASV GP2 core domain. The crystal structure of the CASV GP2 post-fusion conformation indicates a trimeric alpha-helical bundle that is highly similar to those of Ebola virus and Marburg virus GP2 despite CASV genome homology to arenaviruses. Denaturation studies demonstrate that the stability of CASV GP2 is pH dependent with higher stability at lower pH; we propose that this behavior is due to a network of interactions among acidic residues that would destabilize the alpha-helical bundle under conditions where the side chains are deprotonated. The pH-dependent stability of the post-fusion structure has been observed in Ebola virus and Marburg virus GP2, as well as other viruses that enter via the endosome. Infection experiments with CASV and the related Golden Gate virus support a mechanism of entry that requires endosomal acidification. Our results suggest that, despite being primarily arenavirus like, the transmembrane subunit of CASV is extremely similar to the filoviruses.

-	~~==About this Structure==~~	+	Structural Characterization of the Glycoprotein GP2 Core Domain from the CAS Virus, a Novel Arenavirus-Like Species.,Koellhoffer JF, Dai Z, Malashkevich VN, Stenglein MD, Liu Y, Toro R, Harrison J, Chandran K, Derisi JL, Almo SC, Lai JR J Mol Biol. 2013 Dec 12. pii: S0022-2836(13)00751-1. doi:, 10.1016/j.jmb.2013.12.009. PMID:24333483<ref>PMID:24333483</ref>
-	~~[[4n23]] is a 3 chain structure with sequence~~ from ~~[http://en~~.~~wikipedia~~.~~org/wiki/Cas_virus Cas virus]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.~~il/oca-bin/ocashort?id=4N23 OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024333483</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Cas virus]]		[[Category: Cas virus]]
-	[[Category: Almo, S C.]]	+	[[Category: Almo, S C]]
-	[[Category: Dai, Z.]]	+	[[Category: Dai, Z]]
-	[[Category: Koellhoffer, J F.]]	+	[[Category: Koellhoffer, J F]]
-	[[Category: Lai, J R.]]	+	[[Category: Lai, J R]]
-	[[Category: Malashkevich, V N.]]	+	[[Category: Malashkevich, V N]]
-	[[Category: ~~NYSGRC, New York~~ Structural ~~Genomics Research Consortium.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Toro, R.]]	+	[[Category: Toro, R]]
	[[Category: Marburg virus]]		[[Category: Marburg virus]]
-	[[Category: New york structural genomics research consortium]]
	[[Category: Nysgrc]]		[[Category: Nysgrc]]
	[[Category: Post-fusion conformation]]		[[Category: Post-fusion conformation]]
	[[Category: Psi-biology]]		[[Category: Psi-biology]]
-	[[Category: Structural genomic]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 08:10, 8 January 2014

OCA — Wed, 08 Jan 2014 08:10:16 GMT

←Older revision		Revision as of 08:10, 8 January 2014
Line 1:		Line 1:
	{{STRUCTURE_4n23\| PDB=4n23 \| SCENE= }}		{{STRUCTURE_4n23\| PDB=4n23 \| SCENE= }}
	===Crystal structure of the GP2 Core Domain from the California Academy of Science Virus, monoclinic symmetry===		===Crystal structure of the GP2 Core Domain from the California Academy of Science Virus, monoclinic symmetry===
		+	{{ABSTRACT_PUBMED_24333483}}

	==About this Structure==		==About this Structure==
-	[[4n23]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N23 OCA].	+	[[4n23]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Cas_virus Cas virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N23 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024333483</ref><references group="xtra"/><references/>
		+	[[Category: Cas virus]]
	[[Category: Almo, S C.]]		[[Category: Almo, S C.]]
	[[Category: Dai, Z.]]		[[Category: Dai, Z.]]

OCA at 10:36, 27 November 2013

OCA — Wed, 27 Nov 2013 10:36:50 GMT

←Older revision		Revision as of 10:36, 27 November 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4n23\| PDB=4n23 \| SCENE= }}
		+	===Crystal structure of the GP2 Core Domain from the California Academy of Science Virus, monoclinic symmetry===

-	~~The entry~~ 4n23 is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4n23]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N23 OCA].
-	~~Authors~~: ~~Malashkevich, V~~.N.~~, Koellhoffer, J~~.F., Dai, Z., ~~Toro, R~~., Lai, J.R., ~~Almo, S.C~~., New York Structural Genomics Research Consortium ~~(NYSGRC)~~	+	[[Category: Almo, S C.]]
-		+	[[Category: Dai, Z.]]
-	~~Description~~: ~~Crystal structure of the GP2 Core Domain from the California Academy of Science Virus~~, ~~monoclinic symmetry~~	+	[[Category: Koellhoffer, J F.]]
		+	[[Category: Lai, J R.]]
		+	[[Category: Malashkevich, V N.]]
		+	[[Category: NYSGRC, New York Structural Genomics Research Consortium.]]
		+	[[Category: Toro, R.]]
		+	[[Category: Marburg virus]]
		+	[[Category: New york structural genomics research consortium]]
		+	[[Category: Nysgrc]]
		+	[[Category: Post-fusion conformation]]
		+	[[Category: Psi-biology]]
		+	[[Category: Structural genomic]]
		+	[[Category: Viral protein]]

OCA: Protected "4n23" [edit=sysop:move=sysop]

OCA — Wed, 16 Oct 2013 07:31:55 GMT

Protected "4n23" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:31, 16 October 2013

OCA: New page: '''Unreleased structure''' The entry 4n23 is ON HOLD Authors: Malashkevich, V.N., Koellhoffer, J.F., Dai, Z., Toro, R., Lai, J.R., Almo, S.C., New York Structural Genomics Research Cons...

OCA — Wed, 16 Oct 2013 07:31:54 GMT

New page: '''Unreleased structure''' The entry 4n23 is ON HOLD Authors: Malashkevich, V.N., Koellhoffer, J.F., Dai, Z., Toro, R., Lai, J.R., Almo, S.C., New York Structural Genomics Research Cons...

New page

'''Unreleased structure'''

The entry 4n23 is ON HOLD

Authors: Malashkevich, V.N., Koellhoffer, J.F., Dai, Z., Toro, R., Lai, J.R., Almo, S.C., New York Structural Genomics Research Consortium (NYSGRC)

Description: Crystal structure of the GP2 Core Domain from the California Academy of Science Virus, monoclinic symmetry