4n30 - Revision history

OCA at 10:29, 28 December 2022

OCA — Wed, 28 Dec 2022 10:29:01 GMT

←Older revision		Revision as of 10:29, 28 December 2022
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	==Crystal structure of Pseudomonas aeruginosa DsbA2==		==Crystal structure of Pseudomonas aeruginosa DsbA2==
-	<StructureSection load='4n30' size='340' side='right' caption='[[4n30]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='4n30' size='340' side='right'caption='[[4n30]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n30]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N30 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4N30 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4n30]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N30 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N30 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dsbG, EXA31, RL020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n30 OCA], [https://pdbe.org/4n30 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n30 RCSB], [https://www.ebi.ac.uk/pdbsum/4n30 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n30 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4n30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n30 OCA], [~~http~~://pdbe.org/4n30 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4n30 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4n30 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4n30 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q7WY37_PSEAI Q7WY37_PSEAI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Messens, J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Tamu, V D~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Wahni, K~~]]	+	[[Category: Messens J]]
-	[[Category: ~~Oxidoreductase~~]]	+	[[Category: Tamu VD]]
-	[[Category: ~~Thioredoxin fold~~]]	+	[[Category: Wahni K]]

OCA at 10:04, 5 August 2016

OCA — Fri, 05 Aug 2016 10:04:11 GMT

←Older revision		Revision as of 10:04, 5 August 2016
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		+
	==Crystal structure of Pseudomonas aeruginosa DsbA2==		==Crystal structure of Pseudomonas aeruginosa DsbA2==
	<StructureSection load='4n30' size='340' side='right' caption='[[4n30]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='4n30' size='340' side='right' caption='[[4n30]], [[Resolution\|resolution]] 1.30Å' scene=''>
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	<table><tr><td colspan='2'>[[4n30]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N30 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N30 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4n30]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N30 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N30 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dsbG, EXA31, RL020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dsbG, EXA31, RL020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n30 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n30 RCSB], [http://www.ebi.ac.uk/pdbsum/4n30 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n30 OCA], [http://pdbe.org/4n30 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n30 RCSB], [http://www.ebi.ac.uk/pdbsum/4n30 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n30 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4n30" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:35, 21 December 2014

OCA — Sun, 21 Dec 2014 18:35:56 GMT

←Older revision		Revision as of 18:35, 21 December 2014
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-	~~{{STRUCTURE_4n30\| PDB~~=4n30 \| ~~SCENE~~= }}	+	==Crystal structure of Pseudomonas aeruginosa DsbA2==
-	===~~Crystal~~ structure ~~of Pseudomonas aeruginosa DsbA2~~===	+	<StructureSection load='4n30' size='340' side='right' caption='[[4n30]], [[Resolution\|resolution]] 1.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24327342}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4n30]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N30 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N30 FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dsbG, EXA31, RL020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n30 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n30 RCSB], [http://www.ebi.ac.uk/pdbsum/4n30 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Disulfide bond formation is required for the folding of many bacterial virulence factors. However, whereas the Escherichia coli disulfide bond-forming system is well characterized, not much is known on the pathways that oxidatively fold proteins in pathogenic bacteria. Here, we report the detailed unraveling of the pathway that introduces disulfide bonds in the periplasm of the human pathogen Pseudomonas aeruginosa. The genome of P. aeruginosa uniquely encodes two DsbA proteins (P. aeruginosa DsbA1 [PaDsbA1] and PaDsbA2) and two DsbB proteins (PaDsbB1 and PaDsbB2). We found that PaDsbA1, the primary donor of disulfide bonds to secreted proteins, is maintained oxidized in vivo by both PaDsbB1 and PaDsbB2. In vitro reconstitution of the pathway confirms that both PaDsbB1 and PaDsbB2 shuttle electrons from PaDsbA1 to membrane-bound quinones. Accordingly, deletion of both P. aeruginosa dsbB1 (PadsbB1) and PadsbB2 is required to prevent the folding of several P. aeruginosa virulence factors and to lead to a significant decrease in pathogenicity. Using a high-throughput proteomic approach, we also analyzed the impact of PadsbA1 deletion on the global periplasmic proteome of P. aeruginosa, which allowed us to identify more than 20 new potential substrates of this major oxidoreductase. Finally, we report the biochemical and structural characterization of PaDsbA2, a highly oxidizing oxidoreductase, which seems to be expressed under specific conditions. By fully dissecting the machinery that introduces disulfide bonds in P. aeruginosa, our work opens the way to the design of novel antibacterial molecules able to disarm this pathogen by preventing the proper assembly of its arsenal of virulence factors. IMPORTANCE: The human pathogen Pseudomonas aeruginosa causes life-threatening infections in immunodepressed and cystic fibrosis patients. The emergence of P. aeruginosa strains resistant to all of the available antibacterial agents calls for the urgent development of new antibiotics active against this bacterium. The pathogenic power of P. aeruginosa is mediated by an arsenal of extracellular virulence factors, most of which are stabilized by disulfide bonds. Thus, targeting the machinery that introduces disulfide bonds appears to be a promising strategy to combat P. aeruginosa. Here, we unraveled the oxidative protein folding system of P. aeruginosa in full detail. The system uniquely consists of two membrane proteins that generate disulfide bonds de novo to deliver them to P. aeruginosa DsbA1 (PaDsbA1), a soluble oxidoreductase. PaDsbA1 in turn donates disulfide bonds to secreted proteins, including virulence factors. Disruption of the disulfide bond formation machinery dramatically decreases P. aeruginosa virulence, confirming that disulfide formation systems are valid targets for the design of antimicrobial drugs.

-	~~==About this Structure==~~	+	Dissecting the machinery that introduces disulfide bonds in Pseudomonas aeruginosa.,Arts IS, Ball G, Leverrier P, Garvis S, Nicolaes V, Vertommen D, Ize B, Tamu Dufe V, Messens J, Voulhoux R, Collet JF MBio. 2013 Dec 10;4(6):e00912-13. doi: 10.1128/mBio.00912-13. PMID:24327342<ref>PMID:24327342</ref>
-	~~[[4n30]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/"bacillus_aeruginosus"_~~(~~schroeter_1872~~)~~_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4N30 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024327342</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
-	[[Category: Messens, J.]]	+	== References ==
-	[[Category: Tamu, V D.]]	+	<references/>
-	[[Category: Wahni, K.]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Messens, J]]
		+	[[Category: Tamu, V D]]
		+	[[Category: Wahni, K]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Thioredoxin fold]]		[[Category: Thioredoxin fold]]

OCA at 08:19, 8 January 2014

OCA — Wed, 08 Jan 2014 08:19:06 GMT

←Older revision		Revision as of 08:19, 8 January 2014
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	{{STRUCTURE_4n30\| PDB=4n30 \| SCENE= }}		{{STRUCTURE_4n30\| PDB=4n30 \| SCENE= }}
	===Crystal structure of Pseudomonas aeruginosa DsbA2===		===Crystal structure of Pseudomonas aeruginosa DsbA2===
		+	{{ABSTRACT_PUBMED_24327342}}

	==About this Structure==		==About this Structure==
-	[[4n30]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N30 OCA].	+	[[4n30]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N30 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024327342</ref><references group="xtra"/><references/>
	[[Category: Messens, J.]]		[[Category: Messens, J.]]
	[[Category: Tamu, V D.]]		[[Category: Tamu, V D.]]

OCA at 07:34, 4 December 2013

OCA — Wed, 04 Dec 2013 07:34:21 GMT

←Older revision		Revision as of 07:34, 4 December 2013
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-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4n30\| PDB=4n30 \| SCENE= }}
		+	===Crystal structure of Pseudomonas aeruginosa DsbA2===

-	~~The entry~~ 4n30 is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4n30]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N30 OCA].
-	~~Authors~~: ~~Tamu, V~~.D., Messens, J., Wahni, K.	+	[[Category: Messens, J.]]
-		+	[[Category: Tamu, V D.]]
-	~~Description~~: ~~Crystal structure of Pseudomonas aeruginosa DsbA2~~	+	[[Category: Wahni, K.]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Thioredoxin fold]]

OCA at 06:14, 23 October 2013

OCA — Wed, 23 Oct 2013 06:14:06 GMT

←Older revision		Revision as of 06:14, 23 October 2013
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	The entry 4n30 is ON HOLD		The entry 4n30 is ON HOLD

-	Authors: Tamu V, D., Messens J., Wahni K.	+	Authors: Tamu, V.D., Messens, J., Wahni, K.

	Description: Crystal structure of Pseudomonas aeruginosa DsbA2		Description: Crystal structure of Pseudomonas aeruginosa DsbA2

OCA: Protected "4n30" [edit=sysop:move=sysop]

OCA — Wed, 16 Oct 2013 07:32:59 GMT

Protected "4n30" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:32, 16 October 2013

OCA: New page: '''Unreleased structure''' The entry 4n30 is ON HOLD Authors: Tamu V, D., Messens J., Wahni K. Description: Crystal structure of Pseudomonas aeruginosa DsbA2

OCA — Wed, 16 Oct 2013 07:32:58 GMT

New page: '''Unreleased structure''' The entry 4n30 is ON HOLD Authors: Tamu V, D., Messens J., Wahni K. Description: Crystal structure of Pseudomonas aeruginosa DsbA2

New page

'''Unreleased structure'''

The entry 4n30 is ON HOLD

Authors: Tamu V, D., Messens J., Wahni K.

Description: Crystal structure of Pseudomonas aeruginosa DsbA2