4n6e - Revision history

OCA at 12:31, 1 March 2024

OCA — Fri, 01 Mar 2024 12:31:31 GMT

←Older revision		Revision as of 12:31, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4n6e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Amycolatopsis_orientalis_HCCB10007 Amycolatopsis orientalis HCCB10007] and [https://en.wikipedia.org/wiki/Amycolatopsis_orientalis_subsp._vinearia Amycolatopsis orientalis subsp. vinearia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N6E FirstGlance]. <br>		<table><tr><td colspan='2'>[[4n6e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Amycolatopsis_orientalis_HCCB10007 Amycolatopsis orientalis HCCB10007] and [https://en.wikipedia.org/wiki/Amycolatopsis_orientalis_subsp._vinearia Amycolatopsis orientalis subsp. vinearia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N6E FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [https://pdbe.org/4n6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [https://www.ebi.ac.uk/pdbsum/4n6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [https://pdbe.org/4n6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [https://www.ebi.ac.uk/pdbsum/4n6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6e ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/D7RFL7_AMYOR D7RFL7_AMYOR]		[https://www.uniprot.org/uniprot/D7RFL7_AMYOR D7RFL7_AMYOR]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Sulphur is an essential element for life and is ubiquitous in living systems. Yet how the sulphur atom is incorporated into many sulphur-containing secondary metabolites is poorly understood. For bond formation between carbon and sulphur in primary metabolites, the major ionic sulphur sources are the persulphide and thiocarboxylate groups on sulphur-carrier (donor) proteins. Each group is post-translationally generated through the action of a specific activating enzyme. In all reported bacterial cases, the gene encoding the enzyme that catalyses the carbon-sulphur bond formation reaction and that encoding the cognate sulphur-carrier protein exist in the same gene cluster. To study the production of the 2-thiosugar moiety in BE-7585A, an antibiotic from Amycolatopsis orientalis, we identified a putative 2-thioglucose synthase, BexX, whose protein sequence and mode of action seem similar to those of ThiG, the enzyme that catalyses thiazole formation in thiamine biosynthesis. However, no gene encoding a sulphur-carrier protein could be located in the BE-7585A cluster. Subsequent genome sequencing uncovered a few genes encoding sulphur-carrier proteins that are probably involved in the biosynthesis of primary metabolites but only one activating enzyme gene in the A. orientalis genome. Further experiments showed that this activating enzyme can adenylate each of these sulphur-carrier proteins and probably also catalyses the subsequent thiolation, through its rhodanese domain. A proper combination of these sulphur-delivery systems is effective for BexX-catalysed 2-thioglucose production. The ability of BexX to selectively distinguish sulphur-carrier proteins is given a structural basis using X-ray crystallography. This study is, to our knowledge, the first complete characterization of thiosugar formation in nature and also demonstrates the receptor promiscuity of the A. orientalis sulphur-delivery system. Our results also show that co-opting the sulphur-delivery machinery of primary metabolism for the biosynthesis of sulphur-containing natural products is probably a general strategy found in nature.
-
-	Co-opting sulphur-carrier proteins from primary metabolic pathways for 2-thiosugar biosynthesis.,Sasaki E, Zhang X, Sun HG, Lu MY, Liu TL, Ou A, Li JY, Chen YH, Ealick SE, Liu HW Nature. 2014 Jun 19;509(7505):427-31. doi: 10.1038/nature13256. Epub 2014 May 11. PMID:24814342<ref>PMID:24814342</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4n6e" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:11, 11 January 2023

OCA — Wed, 11 Jan 2023 08:11:53 GMT

←Older revision		Revision as of 08:11, 11 January 2023
Line 1:		Line 1:

	==Crystal structure of Amycolatopsis orientalis BexX/CysO complex==		==Crystal structure of Amycolatopsis orientalis BexX/CysO complex==
-	<StructureSection load='4n6e' size='340' side='right' caption='[[4n6e]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='4n6e' size='340' side='right'caption='[[4n6e]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n6e]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"amycolatopsis_orientalis_subsp._vinearia"_okabe_et_al._1990 "amycolatopsis~~ orientalis ~~subsp. vinearia" okabe et al. 1990~~] and [~~http~~://en.wikipedia.org/wiki/~~Amycolatopsis_orientalis_hccb10007~~ Amycolatopsis orientalis ~~hccb10007~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4N6E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4n6e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Amycolatopsis_orientalis_HCCB10007 Amycolatopsis orientalis HCCB10007] and [https://en.wikipedia.org/wiki/Amycolatopsis_orientalis_subsp._vinearia Amycolatopsis orientalis subsp. vinearia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N6E FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n6f\|4n6f]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [https://pdbe.org/4n6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [https://www.ebi.ac.uk/pdbsum/4n6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6e ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bexX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=797057 "Amycolatopsis orientalis subsp. vinearia" Okabe et al. 1990]), AORI_6493 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1156913 Amycolatopsis orientalis HCCB10007])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [~~http~~://pdbe.org/4n6e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4n6e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6e ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D7RFL7_AMYOR D7RFL7_AMYOR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Amycolatopsis orientalis subsp. vinearia ~~okabe et al. 1990~~]]	+	[[Category: Amycolatopsis orientalis HCCB10007]]
-	[[Category: ~~Amycolatopsis orientalis hccb10007~~]]	+	[[Category: Amycolatopsis orientalis subsp. vinearia]]
-	[[Category: Chen, Y]]	+	[[Category: Large Structures]]
-	[[Category: Ealick~~, S E~~]]	+	[[Category: Chen Y]]
-	[[Category: Kinsland, C]]	+	[[Category: Ealick SE]]
-	[[Category: Li, J]]	+	[[Category: Kinsland C]]
-	[[Category: Liu, H]]	+	[[Category: Li J]]
-	[[Category: Liu, T]]	+	[[Category: Liu H]]
-	[[Category: Lu~~, M J~~]]	+	[[Category: Liu T]]
-	[[Category: Ou, A]]	+	[[Category: Lu MJ]]
-	[[Category: Sasaki, E]]	+	[[Category: Ou A]]
-	[[Category: Sun~~, H G~~]]	+	[[Category: Sasaki E]]
-	[[Category: Zhang, X]]	+	[[Category: Sun HG]]
-	[[Category: Zhang, Y]]	+	[[Category: Zhang X]]
-	~~[[Category: Alpha beta barrel]]~~	+	[[Category: Zhang Y]]
-	~~[[Category: Bexx]]~~	+
-	~~[[Category: Cyso]]~~	+
-	~~[[Category: Lyase-biosynthetic protein complex]]~~	+
-	~~[[Category: Protein-protein complex]]~~	+
-	~~[[Category: Sulfur carrier protein]]~~	+
-	~~[[Category: Thiosugar synthase~~]]	+

OCA at 10:02, 15 November 2017

OCA — Wed, 15 Nov 2017 10:02:30 GMT

←Older revision		Revision as of 10:02, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='4n6e' size='340' side='right' caption='[[4n6e]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4n6e' size='340' side='right' caption='[[4n6e]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n6e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N6E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4n6e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"amycolatopsis_orientalis_subsp._vinearia"_okabe_et_al._1990 "amycolatopsis orientalis subsp. vinearia" okabe et al. 1990] and [http://en.wikipedia.org/wiki/Amycolatopsis_orientalis_hccb10007 Amycolatopsis orientalis hccb10007]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N6E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n6f\|4n6f]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n6f\|4n6f]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bexX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=797057 "Amycolatopsis orientalis subsp. vinearia" Okabe et al. 1990]), AORI_6493 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1156913 Amycolatopsis orientalis HCCB10007])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [http://pdbe.org/4n6e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [http://www.ebi.ac.uk/pdbsum/4n6e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [http://pdbe.org/4n6e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [http://www.ebi.ac.uk/pdbsum/4n6e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6e ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Amycolatopsis orientalis subsp. vinearia okabe et al. 1990]]
		+	[[Category: Amycolatopsis orientalis hccb10007]]
	[[Category: Chen, Y]]		[[Category: Chen, Y]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]

OCA at 07:16, 11 August 2016

OCA — Thu, 11 Aug 2016 07:16:20 GMT

←Older revision		Revision as of 07:16, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Amycolatopsis orientalis BexX/CysO complex==		==Crystal structure of Amycolatopsis orientalis BexX/CysO complex==
	<StructureSection load='4n6e' size='340' side='right' caption='[[4n6e]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4n6e' size='340' side='right' caption='[[4n6e]], [[Resolution\|resolution]] 2.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n6f\|4n6f]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n6f\|4n6f]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [http://www.ebi.ac.uk/pdbsum/4n6e PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [http://pdbe.org/4n6e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [http://www.ebi.ac.uk/pdbsum/4n6e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n6e ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4n6e" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:27, 5 January 2015

OCA — Mon, 05 Jan 2015 12:27:26 GMT

←Older revision		Revision as of 12:27, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4n6e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N6E FirstGlance]. <br>		<table><tr><td colspan='2'>[[4n6e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N6E FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n6f\|4n6f]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n6f\|4n6f]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [http://www.ebi.ac.uk/pdbsum/4n6e PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [http://www.ebi.ac.uk/pdbsum/4n6e PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chen, Y.]]	+	[[Category: Chen, Y]]
-	[[Category: Ealick, S E.]]	+	[[Category: Ealick, S E]]
-	[[Category: Kinsland, C.]]	+	[[Category: Kinsland, C]]
-	[[Category: Li, J.]]	+	[[Category: Li, J]]
-	[[Category: Liu, H.]]	+	[[Category: Liu, H]]
-	[[Category: Liu, T.]]	+	[[Category: Liu, T]]
-	[[Category: Lu, M J.]]	+	[[Category: Lu, M J]]
-	[[Category: Ou, A.]]	+	[[Category: Ou, A]]
-	[[Category: Sasaki, E.]]	+	[[Category: Sasaki, E]]
-	[[Category: Sun, H G.]]	+	[[Category: Sun, H G]]
-	[[Category: Zhang, X.]]	+	[[Category: Zhang, X]]
-	[[Category: Zhang, Y.]]	+	[[Category: Zhang, Y]]
	[[Category: Alpha beta barrel]]		[[Category: Alpha beta barrel]]
	[[Category: Bexx]]		[[Category: Bexx]]

OCA at 05:15, 25 June 2014

OCA — Wed, 25 Jun 2014 05:15:20 GMT

←Older revision		Revision as of 05:15, 25 June 2014
Line 2:		Line 2:
	<StructureSection load='4n6e' size='340' side='right' caption='[[4n6e]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4n6e' size='340' side='right' caption='[[4n6e]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n6e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6E OCA]. <br>	+	<table><tr><td colspan='2'>[[4n6e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N6E FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n6f\|4n6f]]</td></tr>		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n6f\|4n6f]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [http://www.ebi.ac.uk/pdbsum/4n6e PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [http://www.ebi.ac.uk/pdbsum/4n6e PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sulphur is an essential element for life and is ubiquitous in living systems. Yet how the sulphur atom is incorporated into many sulphur-containing secondary metabolites is poorly understood. For bond formation between carbon and sulphur in primary metabolites, the major ionic sulphur sources are the persulphide and thiocarboxylate groups on sulphur-carrier (donor) proteins. Each group is post-translationally generated through the action of a specific activating enzyme. In all reported bacterial cases, the gene encoding the enzyme that catalyses the carbon-sulphur bond formation reaction and that encoding the cognate sulphur-carrier protein exist in the same gene cluster. To study the production of the 2-thiosugar moiety in BE-7585A, an antibiotic from Amycolatopsis orientalis, we identified a putative 2-thioglucose synthase, BexX, whose protein sequence and mode of action seem similar to those of ThiG, the enzyme that catalyses thiazole formation in thiamine biosynthesis. However, no gene encoding a sulphur-carrier protein could be located in the BE-7585A cluster. Subsequent genome sequencing uncovered a few genes encoding sulphur-carrier proteins that are probably involved in the biosynthesis of primary metabolites but only one activating enzyme gene in the A. orientalis genome. Further experiments showed that this activating enzyme can adenylate each of these sulphur-carrier proteins and probably also catalyses the subsequent thiolation, through its rhodanese domain. A proper combination of these sulphur-delivery systems is effective for BexX-catalysed 2-thioglucose production. The ability of BexX to selectively distinguish sulphur-carrier proteins is given a structural basis using X-ray crystallography. This study is, to our knowledge, the first complete characterization of thiosugar formation in nature and also demonstrates the receptor promiscuity of the A. orientalis sulphur-delivery system. Our results also show that co-opting the sulphur-delivery machinery of primary metabolism for the biosynthesis of sulphur-containing natural products is probably a general strategy found in nature.
		+
		+	Co-opting sulphur-carrier proteins from primary metabolic pathways for 2-thiosugar biosynthesis.,Sasaki E, Zhang X, Sun HG, Lu MY, Liu TL, Ou A, Li JY, Chen YH, Ealick SE, Liu HW Nature. 2014 Jun 19;509(7505):427-31. doi: 10.1038/nature13256. Epub 2014 May 11. PMID:24814342<ref>PMID:24814342</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:26, 14 May 2014

OCA — Wed, 14 May 2014 07:26:08 GMT

←Older revision		Revision as of 07:26, 14 May 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of Amycolatopsis orientalis BexX/CysO complex==
-		+	<StructureSection load='4n6e' size='340' side='right' caption='[[4n6e]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~The entry~~ 4n6e is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4n6e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N6E OCA]. <br>
-	~~Authors~~: ~~Zhang~~, X., ~~Zhang~~, Y., ~~Kinsland~~, C., ~~Sasaki,~~ E., ~~Sun, H.G~~., ~~Lu, M.~~J., Liu, T., Ou, A., ~~Li, J~~., ~~Chen, Y~~., ~~Liu, H~~., ~~Ealick, S.E~~.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n6f\|4n6f]]</td></tr>
-	~~Description~~: ~~Crystal structure of Amycolatopsis orientalis BexX/CysO~~ complex	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n6e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n6e RCSB], [http://www.ebi.ac.uk/pdbsum/4n6e PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, Y.]]
		+	[[Category: Ealick, S E.]]
		+	[[Category: Kinsland, C.]]
		+	[[Category: Li, J.]]
		+	[[Category: Liu, H.]]
		+	[[Category: Liu, T.]]
		+	[[Category: Lu, M J.]]
		+	[[Category: Ou, A.]]
		+	[[Category: Sasaki, E.]]
		+	[[Category: Sun, H G.]]
		+	[[Category: Zhang, X.]]
		+	[[Category: Zhang, Y.]]
		+	[[Category: Alpha beta barrel]]
		+	[[Category: Bexx]]
		+	[[Category: Cyso]]
		+	[[Category: Lyase-biosynthetic protein complex]]
		+	[[Category: Protein-protein complex]]
		+	[[Category: Sulfur carrier protein]]
		+	[[Category: Thiosugar synthase]]

OCA at 07:40, 13 November 2013

OCA — Wed, 13 Nov 2013 07:40:27 GMT

←Older revision		Revision as of 07:40, 13 November 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4n6e is ON HOLD	+	The entry 4n6e is ON HOLD until Paper Publication

	Authors: Zhang, X., Zhang, Y., Kinsland, C., Sasaki, E., Sun, H.G., Lu, M.J., Liu, T., Ou, A., Li, J., Chen, Y., Liu, H., Ealick, S.E.		Authors: Zhang, X., Zhang, Y., Kinsland, C., Sasaki, E., Sun, H.G., Lu, M.J., Liu, T., Ou, A., Li, J., Chen, Y., Liu, H., Ealick, S.E.

	Description: Crystal structure of Amycolatopsis orientalis BexX/CysO complex		Description: Crystal structure of Amycolatopsis orientalis BexX/CysO complex

OCA: Protected "4n6e" [edit=sysop:move=sysop]

OCA — Wed, 23 Oct 2013 06:15:47 GMT

Protected "4n6e" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:15, 23 October 2013

OCA: New page: '''Unreleased structure''' The entry 4n6e is ON HOLD Authors: Zhang, X., Zhang, Y., Kinsland, C., Sasaki, E., Sun, H.G., Lu, M.J., Liu, T., Ou, A., Li, J., Chen, Y., Liu, H., Ealick, S....

OCA — Wed, 23 Oct 2013 06:15:46 GMT

New page: '''Unreleased structure''' The entry 4n6e is ON HOLD Authors: Zhang, X., Zhang, Y., Kinsland, C., Sasaki, E., Sun, H.G., Lu, M.J., Liu, T., Ou, A., Li, J., Chen, Y., Liu, H., Ealick, S....

New page

'''Unreleased structure'''

The entry 4n6e is ON HOLD

Authors: Zhang, X., Zhang, Y., Kinsland, C., Sasaki, E., Sun, H.G., Lu, M.J., Liu, T., Ou, A., Li, J., Chen, Y., Liu, H., Ealick, S.E.

Description: Crystal structure of Amycolatopsis orientalis BexX/CysO complex