4n7u - Revision history

OCA at 14:07, 13 March 2024

OCA — Wed, 13 Mar 2024 14:07:24 GMT

←Older revision		Revision as of 14:07, 13 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/BT3A1_HUMAN BT3A1_HUMAN] Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.<ref>PMID:21113407</ref> <ref>PMID:21918970</ref> <ref>PMID:22767497</ref> <ref>PMID:22846996</ref>		[https://www.uniprot.org/uniprot/BT3A1_HUMAN BT3A1_HUMAN] Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.<ref>PMID:21113407</ref> <ref>PMID:21918970</ref> <ref>PMID:22767497</ref> <ref>PMID:22846996</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In humans, Vgamma9Vdelta2 T cells detect tumor cells and microbial infections, including Mycobacterium tuberculosis, through recognition of small pyrophosphate containing organic molecules known as phosphoantigens (pAgs). Key to pAg-mediated activation of Vgamma9Vdelta2 T cells is the butyrophilin 3A1 (BTN3A1) protein that contains an intracellular B30.2 domain critical to pAg reactivity. Here, we have demonstrated through structural, biophysical, and functional approaches that the intracellular B30.2 domain of BTN3A1 directly binds pAg through a positively charged surface pocket. Charge reversal of pocket residues abrogates binding and Vgamma9Vdelta2 T cell activation. We have also identified a gain-of-function mutation within this pocket that, when introduced into the B30.2 domain of the nonstimulatory BTN3A3 isoform, transfers pAg binding ability and Vgamma9Vdelta2 T cell activation. These studies demonstrate that internal sensing of changes in pAg metabolite concentrations by BTN3A1 molecules is a critical step in Vgamma9Vdelta2 T cell detection of infection and tumorigenesis.
-
-	The Intracellular B30.2 Domain of Butyrophilin 3A1 Binds Phosphoantigens to Mediate Activation of Human Vgamma9Vdelta2 T Cells.,Sandstrom A, Peigne CM, Leger A, Crooks JE, Konczak F, Gesnel MC, Breathnach R, Bonneville M, Scotet E, Adams EJ Immunity. 2014 Apr 17;40(4):490-500. doi: 10.1016/j.immuni.2014.03.003. Epub 2014, Apr 3. PMID:24703779<ref>PMID:24703779</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4n7u" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:49, 20 September 2023

OCA — Wed, 20 Sep 2023 16:49:11 GMT

←Older revision		Revision as of 16:49, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4n7u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N7U FirstGlance]. <br>		<table><tr><td colspan='2'>[[4n7u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N7U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2JA:[(E)-4-METHYL-5-OXIDANYL-PENT-3-ENYL]-PHOSPHONOOXY-PHOSPHINIC+ACID'>2JA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4598Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2JA:[(E)-4-METHYL-5-OXIDANYL-PENT-3-ENYL]-PHOSPHONOOXY-PHOSPHINIC+ACID'>2JA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7u OCA], [https://pdbe.org/4n7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n7u RCSB], [https://www.ebi.ac.uk/pdbsum/4n7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n7u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7u OCA], [https://pdbe.org/4n7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n7u RCSB], [https://www.ebi.ac.uk/pdbsum/4n7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n7u ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:13, 11 January 2023

OCA — Wed, 11 Jan 2023 08:13:48 GMT

←Older revision		Revision as of 08:13, 11 January 2023
Line 1:		Line 1:

	==Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP==		==Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP==
-	<StructureSection load='4n7u' size='340' side='right' caption='[[4n7u]], [[Resolution\|resolution]] 1.46Å' scene=''>	+	<StructureSection load='4n7u' size='340' side='right'caption='[[4n7u]], [[Resolution\|resolution]] 1.46Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n7u]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N7U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4N7U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4n7u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N7U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2JA:[(E)-4-METHYL-5-OXIDANYL-PENT-3-ENYL]-PHOSPHONOOXY-PHOSPHINIC+ACID'>2JA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2JA:[(E)-4-METHYL-5-OXIDANYL-PENT-3-ENYL]-PHOSPHONOOXY-PHOSPHINIC+ACID'>2JA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n7i\|4n7i]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7u OCA], [https://pdbe.org/4n7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n7u RCSB], [https://www.ebi.ac.uk/pdbsum/4n7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n7u ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTF5, BTN3A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4n7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7u OCA], [~~http~~://pdbe.org/4n7u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4n7u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4n7u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4n7u ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/BT3A1_HUMAN BT3A1_HUMAN]] Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.<ref>PMID:21113407</ref> <ref>PMID:21918970</ref> <ref>PMID:22767497</ref> <ref>PMID:22846996</ref>	+	[https://www.uniprot.org/uniprot/BT3A1_HUMAN BT3A1_HUMAN] Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.<ref>PMID:21113407</ref> <ref>PMID:21918970</ref> <ref>PMID:22767497</ref> <ref>PMID:22846996</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Adams, E J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Sandstrom, A~~]]	+	[[Category: Adams EJ]]
-	[[Category: ~~B30 2]]~~	+	[[Category: Sandstrom A]]
-	~~[[Category: Butyrophilin]]~~	+
-	~~[[Category: Cd277]]~~	+
-	~~[[Category: Phosphoantigen]]~~	+
-	~~[[Category: Pry/spry]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 23:53, 4 August 2016

OCA — Thu, 04 Aug 2016 23:53:26 GMT

←Older revision		Revision as of 23:53, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP==		==Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP==
	<StructureSection load='4n7u' size='340' side='right' caption='[[4n7u]], [[Resolution\|resolution]] 1.46Å' scene=''>		<StructureSection load='4n7u' size='340' side='right' caption='[[4n7u]], [[Resolution\|resolution]] 1.46Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n7i\|4n7i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n7i\|4n7i]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTF5, BTN3A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTF5, BTN3A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n7u RCSB], [http://www.ebi.ac.uk/pdbsum/4n7u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7u OCA], [http://pdbe.org/4n7u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n7u RCSB], [http://www.ebi.ac.uk/pdbsum/4n7u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n7u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4n7u" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:09, 25 December 2014

OCA — Thu, 25 Dec 2014 14:09:42 GMT

←Older revision		Revision as of 14:09, 25 December 2014
Line 2:		Line 2:
	<StructureSection load='4n7u' size='340' side='right' caption='[[4n7u]], [[Resolution\|resolution]] 1.46Å' scene=''>		<StructureSection load='4n7u' size='340' side='right' caption='[[4n7u]], [[Resolution\|resolution]] 1.46Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n7u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N7U OCA]. <br>	+	<table><tr><td colspan='2'>[[4n7u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N7U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N7U FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2JA:[(E)-4-METHYL-5-OXIDANYL-PENT-3-ENYL]-PHOSPHONOOXY-PHOSPHINIC+ACID'>2JA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2JA:[(E)-4-METHYL-5-OXIDANYL-PENT-3-ENYL]-PHOSPHONOOXY-PHOSPHINIC+ACID'>2JA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n7i\|4n7i]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n7i\|4n7i]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTF5, BTN3A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTF5, BTN3A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr~~><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class~~='~~plainlinks~~'~~>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n7u RCSB], [http://www.ebi.ac.uk/pdbsum/4n7u PDBsum]</span></td></tr>
-	~~<tr~~><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n7u RCSB], [http://www.ebi.ac.uk/pdbsum/4n7u PDBsum]</span></td></tr>	+	</table>
-	<table>	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BT3A1_HUMAN BT3A1_HUMAN]] Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.<ref>PMID:21113407</ref> <ref>PMID:21918970</ref> <ref>PMID:22767497</ref> <ref>PMID:22846996</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 16:
	The Intracellular B30.2 Domain of Butyrophilin 3A1 Binds Phosphoantigens to Mediate Activation of Human Vgamma9Vdelta2 T Cells.,Sandstrom A, Peigne CM, Leger A, Crooks JE, Konczak F, Gesnel MC, Breathnach R, Bonneville M, Scotet E, Adams EJ Immunity. 2014 Apr 17;40(4):490-500. doi: 10.1016/j.immuni.2014.03.003. Epub 2014, Apr 3. PMID:24703779<ref>PMID:24703779</ref>		The Intracellular B30.2 Domain of Butyrophilin 3A1 Binds Phosphoantigens to Mediate Activation of Human Vgamma9Vdelta2 T Cells.,Sandstrom A, Peigne CM, Leger A, Crooks JE, Konczak F, Gesnel MC, Breathnach R, Bonneville M, Scotet E, Adams EJ Immunity. 2014 Apr 17;40(4):490-500. doi: 10.1016/j.immuni.2014.03.003. Epub 2014, Apr 3. PMID:24703779<ref>PMID:24703779</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
-	[[Category: Adams, E J.]]	+	[[Category: Adams, E J]]
-	[[Category: Sandstrom, A.]]	+	[[Category: Sandstrom, A]]
	[[Category: B30 2]]		[[Category: B30 2]]
	[[Category: Butyrophilin]]		[[Category: Butyrophilin]]

OCA at 06:37, 14 May 2014

OCA — Wed, 14 May 2014 06:37:39 GMT

←Older revision		Revision as of 06:37, 14 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4n7u\| PDB=4n7u \| SCENE= }}~~	+	==Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP==
-	===Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP===	+	<StructureSection load='4n7u' size='340' side='right' caption='[[4n7u]], [[Resolution\|resolution]] 1.46Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24703779}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4n7u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N7U OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2JA:[(E)-4-METHYL-5-OXIDANYL-PENT-3-ENYL]-PHOSPHONOOXY-PHOSPHINIC+ACID'>2JA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n7i\|4n7i]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BTF5, BTN3A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n7u RCSB], [http://www.ebi.ac.uk/pdbsum/4n7u PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In humans, Vgamma9Vdelta2 T cells detect tumor cells and microbial infections, including Mycobacterium tuberculosis, through recognition of small pyrophosphate containing organic molecules known as phosphoantigens (pAgs). Key to pAg-mediated activation of Vgamma9Vdelta2 T cells is the butyrophilin 3A1 (BTN3A1) protein that contains an intracellular B30.2 domain critical to pAg reactivity. Here, we have demonstrated through structural, biophysical, and functional approaches that the intracellular B30.2 domain of BTN3A1 directly binds pAg through a positively charged surface pocket. Charge reversal of pocket residues abrogates binding and Vgamma9Vdelta2 T cell activation. We have also identified a gain-of-function mutation within this pocket that, when introduced into the B30.2 domain of the nonstimulatory BTN3A3 isoform, transfers pAg binding ability and Vgamma9Vdelta2 T cell activation. These studies demonstrate that internal sensing of changes in pAg metabolite concentrations by BTN3A1 molecules is a critical step in Vgamma9Vdelta2 T cell detection of infection and tumorigenesis.

-	~~==Function==~~	+	The Intracellular B30.2 Domain of Butyrophilin 3A1 Binds Phosphoantigens to Mediate Activation of Human Vgamma9Vdelta2 T Cells.,Sandstrom A, Peigne CM, Leger A, Crooks JE, Konczak F, Gesnel MC, Breathnach R, Bonneville M, Scotet E, Adams EJ Immunity. 2014 Apr 17;40(4):490-500. doi: 10.1016/j.immuni.2014.03.003. Epub 2014, Apr 3. PMID:24703779<ref>PMID:24703779</ref>
-	~~[[http://www~~.~~uniprot.org/uniprot/BT3A1_HUMAN BT3A1_HUMAN]] Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation~~ of ~~activated T-cells. Regulates the release~~ of ~~cytokines and IFNG by activated~~ T~~-cells~~. ~~Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites~~, ~~such as isopentenyl pyrophosphate~~.~~<ref>PMID~~:~~21113407</ref> <ref>PMID~~:~~21918970<~~/~~ref> <ref>~~PMID:~~22767497</ref>~~ <ref>PMID:~~22846996~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4n7u]] is~~ a ~~1 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4N7U OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:024703779</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
	[[Category: Adams, E J.]]		[[Category: Adams, E J.]]
	[[Category: Sandstrom, A.]]		[[Category: Sandstrom, A.]]

OCA at 07:37, 23 April 2014

OCA — Wed, 23 Apr 2014 07:37:35 GMT

←Older revision		Revision as of 07:37, 23 April 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4n7u\| PDB=4n7u \| SCENE= }}
		+	===Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP===
		+	{{ABSTRACT_PUBMED_24703779}}

-	~~The entry 4n7u is ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/BT3A1_HUMAN BT3A1_HUMAN]] Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.<ref>PMID:21113407</ref> <ref>PMID:21918970</ref> <ref>PMID:22767497</ref> <ref>PMID:22846996</ref>

-	~~Authors~~: ~~Sandstrom, A~~.~~, Adams, E~~.J.	+	==About this Structure==
		+	[[4n7u]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N7U OCA].

-	~~Description~~: ~~Crystal Structure of Intracellular B30~~.2 ~~Domain of BTN3A1 in Complex with CHDMAPP~~	+	==Reference==
		+	<ref group="xtra">PMID:024703779</ref><references group="xtra"/><references/>
		+	[[Category: Adams, E J.]]
		+	[[Category: Sandstrom, A.]]
		+	[[Category: B30 2]]
		+	[[Category: Butyrophilin]]
		+	[[Category: Cd277]]
		+	[[Category: Phosphoantigen]]
		+	[[Category: Pry/spry]]
		+	[[Category: Signaling protein]]

OCA at 09:45, 27 November 2013

OCA — Wed, 27 Nov 2013 09:45:50 GMT

←Older revision		Revision as of 09:45, 27 November 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4n7u is ON HOLD	+	The entry 4n7u is ON HOLD until Paper Publication

	Authors: Sandstrom, A., Adams, E.J.		Authors: Sandstrom, A., Adams, E.J.

	Description: Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP		Description: Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP

OCA: Protected "4n7u" [edit=sysop:move=sysop]

OCA — Wed, 23 Oct 2013 06:17:41 GMT

Protected "4n7u" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:17, 23 October 2013

OCA: New page: '''Unreleased structure''' The entry 4n7u is ON HOLD Authors: Sandstrom, A., Adams, E.J. Description: Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP

OCA — Wed, 23 Oct 2013 06:17:40 GMT

New page: '''Unreleased structure''' The entry 4n7u is ON HOLD Authors: Sandstrom, A., Adams, E.J. Description: Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP

New page

'''Unreleased structure'''

The entry 4n7u is ON HOLD

Authors: Sandstrom, A., Adams, E.J.

Description: Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP