4n80 - Revision history

OCA at 14:46, 8 November 2023

OCA — Wed, 08 Nov 2023 14:46:20 GMT

←Older revision		Revision as of 14:46, 8 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4n80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N80 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4n80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N80 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [https://pdbe.org/4n80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [https://www.ebi.ac.uk/pdbsum/4n80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n80 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [https://pdbe.org/4n80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [https://www.ebi.ac.uk/pdbsum/4n80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n80 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:13, 11 January 2023

OCA — Wed, 11 Jan 2023 08:13:59 GMT

←Older revision		Revision as of 08:13, 11 January 2023
Line 1:		Line 1:

	==Crystal structure of Tse3-Tsi3 complex==		==Crystal structure of Tse3-Tsi3 complex==
-	<StructureSection load='4n80' size='340' side='right' caption='[[4n80]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='4n80' size='340' side='right'caption='[[4n80]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n80]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4N80 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4n80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N80 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [https://pdbe.org/4n80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [https://www.ebi.ac.uk/pdbsum/4n80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n80 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA3484 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE]), PA3485 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [~~http~~://pdbe.org/4n80 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4n80 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4n80 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TSE3_PSEAE TSE3_PSEAE] Toxin secreted by the H1 type VI (H1-T6SS) secretion system into the periplasm of recipient cells. Degrades peptidoglycan via muramidase activity thereby helping itself to compete with other bacteria (PubMed:21776080). To protect itself, the bacterium synthesizes immunity protein Tsi3 that specifically interacts with and inactivates cognate toxin (PubMed:24025333).<ref>PMID:21776080</ref> <ref>PMID:24025333</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseae~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Shang, G J~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: ~~Hydrolase-hydrolase inhibitor complex]]~~	+	[[Category: Shang GJ]]
-	~~[[Category: Lysozyme fold~~]]	+

OCA at 18:47, 11 August 2016

OCA — Thu, 11 Aug 2016 18:47:47 GMT

←Older revision		Revision as of 18:47, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Tse3-Tsi3 complex==		==Crystal structure of Tse3-Tsi3 complex==
	<StructureSection load='4n80' size='340' side='right' caption='[[4n80]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4n80' size='340' side='right' caption='[[4n80]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA3484 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE]), PA3485 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA3484 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE]), PA3485 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [http://www.ebi.ac.uk/pdbsum/4n80 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [http://pdbe.org/4n80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [http://www.ebi.ac.uk/pdbsum/4n80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n80 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4n80" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:17, 5 January 2015

OCA — Mon, 05 Jan 2015 09:17:26 GMT

←Older revision		Revision as of 09:17, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4n80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N80 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4n80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N80 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA3484 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE]), PA3485 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA3484 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE]), PA3485 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [http://www.ebi.ac.uk/pdbsum/4n80 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [http://www.ebi.ac.uk/pdbsum/4n80 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Pseae]]		[[Category: Pseae]]
-	[[Category: Shang, G J.]]	+	[[Category: Shang, G J]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Lysozyme fold]]		[[Category: Lysozyme fold]]

OCA at 05:39, 18 June 2014

OCA — Wed, 18 Jun 2014 05:39:09 GMT

←Older revision		Revision as of 05:39, 18 June 2014
Line 2:		Line 2:
	<StructureSection load='4n80' size='340' side='right' caption='[[4n80]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4n80' size='340' side='right' caption='[[4n80]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. <br>	+	<table><tr><td colspan='2'>[[4n80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N80 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]</td></tr>		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]</td></tr>
-	<tr><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat">~~<span class='plainlinks'>~~[http://en.~~wikipedia~~.~~org~~/~~wiki~~/~~Glucokinase Glucokinase~~], ~~with EC number~~ [http://www.~~brenda-enzymes~~.~~info~~/~~php~~/~~result_flat~~.~~php4~~?~~ecno~~=~~2.7.1.2 2.7.1.2~~] ~~</span>~~</td></tr>	+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA3484 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE]), PA3485 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [http://www.ebi.ac.uk/pdbsum/4n80 PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [http://www.ebi.ac.uk/pdbsum/4n80 PDBsum]</span></td></tr>
	<table>		<table>
Line 14:		Line 14:
	Structural insights into the T6SS effector protein Tse3 and the Tse3-Tsi3 complex from Pseudomonas aeruginosa reveal a calcium-dependent membrane-binding mechanism.,Lu D, Shang G, Zhang H, Yu Q, Cong X, Yuan J, He F, Zhu C, Zhao Y, Yin K, Chen Y, Hu J, Zhang X, Yuan Z, Xu S, Hu W, Cang H, Gu L Mol Microbiol. 2014 Apr 14. doi: 10.1111/mmi.12616. PMID:24724564<ref>PMID:24724564</ref>		Structural insights into the T6SS effector protein Tse3 and the Tse3-Tsi3 complex from Pseudomonas aeruginosa reveal a calcium-dependent membrane-binding mechanism.,Lu D, Shang G, Zhang H, Yu Q, Cong X, Yuan J, He F, Zhu C, Zhao Y, Yin K, Chen Y, Hu J, Zhang X, Yuan Z, Xu S, Hu W, Cang H, Gu L Mol Microbiol. 2014 Apr 14. doi: 10.1111/mmi.12616. PMID:24724564<ref>PMID:24724564</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Pseae]]
	[[Category: Shang, G J.]]		[[Category: Shang, G J.]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Lysozyme fold]]		[[Category: Lysozyme fold]]

OCA at 09:41, 1 May 2014

OCA — Thu, 01 May 2014 09:41:30 GMT

←Older revision		Revision as of 09:41, 1 May 2014
Line 2:		Line 2:
	<StructureSection load='4n80' size='340' side='right' caption='[[4n80]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4n80' size='340' side='right' caption='[[4n80]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	[[4n80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. <br>	+	<table><tr><td colspan='2'>[[4n80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. <br>
-	<b>[[Ligand\|Ligands:]]</b> <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
-	<b>[[Related_structure\|Related:]]</b> [[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]<br>	+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]</td></tr>
-	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
-	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [http://www.ebi.ac.uk/pdbsum/4n80 PDBsum]</span><br>	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [http://www.ebi.ac.uk/pdbsum/4n80 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	The opportunistic pathogen Pseudomonas aeruginosa uses the type VI secretion system (T6SS) to deliver the muramidase Tse3 into the periplasm of rival bacteria to degrade their peptidoglycan (PG). Concomitantly, P. aeruginosa uses the periplasm-localized immunity protein Tsi3 to prevent potential self-intoxication caused by Tse3, and thus gains an edge over rival bacteria in fierce niche competition. Here, we report the crystal structures of Tse3 and the Tse3-Tsi3 complex. Tse3 contains an annexin repeat-like fold at the N-terminus and a G-type lysozyme fold at the C-terminus. One loop in the N-terminal domain (Loop 12) and one helix (alpha9) from the C-terminal domain together anchor Tse3 and the Tse3-Tsi3 complex to membrane in a calcium-dependent manner in vitro, and this membrane-binding ability is essential for Tse3's activity. In the C-terminal domain, a Y-shaped groove present on the surface likely serves as the PG-binding site. Two calcium-binding motifs are also observed in the groove and these are necessary for Tse3 activity. In the Tse3-Tsi3 structure, three loops of Tsi3 insert into the substrate-binding groove of Tse3, and three calcium ions present at the interface of the complex are indispensable for the formation of the Tse3-Tsi3 complex.		The opportunistic pathogen Pseudomonas aeruginosa uses the type VI secretion system (T6SS) to deliver the muramidase Tse3 into the periplasm of rival bacteria to degrade their peptidoglycan (PG). Concomitantly, P. aeruginosa uses the periplasm-localized immunity protein Tsi3 to prevent potential self-intoxication caused by Tse3, and thus gains an edge over rival bacteria in fierce niche competition. Here, we report the crystal structures of Tse3 and the Tse3-Tsi3 complex. Tse3 contains an annexin repeat-like fold at the N-terminus and a G-type lysozyme fold at the C-terminus. One loop in the N-terminal domain (Loop 12) and one helix (alpha9) from the C-terminal domain together anchor Tse3 and the Tse3-Tsi3 complex to membrane in a calcium-dependent manner in vitro, and this membrane-binding ability is essential for Tse3's activity. In the C-terminal domain, a Y-shaped groove present on the surface likely serves as the PG-binding site. Two calcium-binding motifs are also observed in the groove and these are necessary for Tse3 activity. In the Tse3-Tsi3 structure, three loops of Tsi3 insert into the substrate-binding groove of Tse3, and three calcium ions present at the interface of the complex are indispensable for the formation of the Tse3-Tsi3 complex.
Line 13:		Line 15:

	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:15, 30 April 2014

OCA — Wed, 30 Apr 2014 10:15:26 GMT

←Older revision		Revision as of 10:15, 30 April 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	[[4n80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. <br>		[[4n80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. <br>
-	<b>Related:</b> [[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]<br>	+	<b>[[Ligand\|Ligands:]]</b> <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<b>[[Related_structure\|Related:]]</b> [[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]<br>
	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>		<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n80 RCSB], [http://www.ebi.ac.uk/pdbsum/4n80 PDBsum]</span><br>
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	The opportunistic pathogen Pseudomonas aeruginosa uses the type VI secretion system (T6SS) to deliver the muramidase Tse3 into the periplasm of rival bacteria to degrade their peptidoglycan (PG). Concomitantly, P. aeruginosa uses the periplasm-localized immunity protein Tsi3 to prevent potential self-intoxication caused by Tse3, and thus gains an edge over rival bacteria in fierce niche competition. Here, we report the crystal structures of Tse3 and the Tse3-Tsi3 complex. Tse3 contains an annexin repeat-like fold at the N-terminus and a G-type lysozyme fold at the C-terminus. One loop in the N-terminal domain (Loop 12) and one helix (alpha9) from the C-terminal domain together anchor Tse3 and the Tse3-Tsi3 complex to membrane in a calcium-dependent manner in vitro, and this membrane-binding ability is essential for Tse3's activity. In the C-terminal domain, a Y-shaped groove present on the surface likely serves as the PG-binding site. Two calcium-binding motifs are also observed in the groove and these are necessary for Tse3 activity. In the Tse3-Tsi3 structure, three loops of Tsi3 insert into the substrate-binding groove of Tse3, and three calcium ions present at the interface of the complex are indispensable for the formation of the Tse3-Tsi3 complex.		The opportunistic pathogen Pseudomonas aeruginosa uses the type VI secretion system (T6SS) to deliver the muramidase Tse3 into the periplasm of rival bacteria to degrade their peptidoglycan (PG). Concomitantly, P. aeruginosa uses the periplasm-localized immunity protein Tsi3 to prevent potential self-intoxication caused by Tse3, and thus gains an edge over rival bacteria in fierce niche competition. Here, we report the crystal structures of Tse3 and the Tse3-Tsi3 complex. Tse3 contains an annexin repeat-like fold at the N-terminus and a G-type lysozyme fold at the C-terminus. One loop in the N-terminal domain (Loop 12) and one helix (alpha9) from the C-terminal domain together anchor Tse3 and the Tse3-Tsi3 complex to membrane in a calcium-dependent manner in vitro, and this membrane-binding ability is essential for Tse3's activity. In the C-terminal domain, a Y-shaped groove present on the surface likely serves as the PG-binding site. Two calcium-binding motifs are also observed in the groove and these are necessary for Tse3 activity. In the Tse3-Tsi3 structure, three loops of Tsi3 insert into the substrate-binding groove of Tse3, and three calcium ions present at the interface of the complex are indispensable for the formation of the Tse3-Tsi3 complex.

OCA at 05:25, 30 April 2014

OCA — Wed, 30 Apr 2014 05:25:22 GMT

←Older revision		Revision as of 05:25, 30 April 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4n80~~\| ~~PDB~~=4n80 \| ~~SCENE~~= }}	+	==Crystal structure of Tse3-Tsi3 complex==
-	===~~Crystal~~ structure of Tse3-Tsi3 complex~~===~~	+	<StructureSection load='4n80' size='340' side='right' caption='[[4n80]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24724564}}~~	+	== Structural highlights ==
		+	[[4n80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA]. <br>
		+	<b>Related:</b> [[4m5e\|4m5e]], [[4n7s\|4n7s]], [[4m5f\|4m5f]], [[4n88\|4n88]]<br>
		+	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	== Publication Abstract from PubMed ==
		+	The opportunistic pathogen Pseudomonas aeruginosa uses the type VI secretion system (T6SS) to deliver the muramidase Tse3 into the periplasm of rival bacteria to degrade their peptidoglycan (PG). Concomitantly, P. aeruginosa uses the periplasm-localized immunity protein Tsi3 to prevent potential self-intoxication caused by Tse3, and thus gains an edge over rival bacteria in fierce niche competition. Here, we report the crystal structures of Tse3 and the Tse3-Tsi3 complex. Tse3 contains an annexin repeat-like fold at the N-terminus and a G-type lysozyme fold at the C-terminus. One loop in the N-terminal domain (Loop 12) and one helix (alpha9) from the C-terminal domain together anchor Tse3 and the Tse3-Tsi3 complex to membrane in a calcium-dependent manner in vitro, and this membrane-binding ability is essential for Tse3's activity. In the C-terminal domain, a Y-shaped groove present on the surface likely serves as the PG-binding site. Two calcium-binding motifs are also observed in the groove and these are necessary for Tse3 activity. In the Tse3-Tsi3 structure, three loops of Tsi3 insert into the substrate-binding groove of Tse3, and three calcium ions present at the interface of the complex are indispensable for the formation of the Tse3-Tsi3 complex.

-	~~==About this Structure==~~	+	Structural insights into the T6SS effector protein Tse3 and the Tse3-Tsi3 complex from Pseudomonas aeruginosa reveal a calcium-dependent membrane-binding mechanism.,Lu D, Shang G, Zhang H, Yu Q, Cong X, Yuan J, He F, Zhu C, Zhao Y, Yin K, Chen Y, Hu J, Zhang X, Yuan Z, Xu S, Hu W, Cang H, Gu L Mol Microbiol. 2014 Apr 14. doi: 10.1111/mmi.12616. PMID:24724564<ref>PMID:24724564</ref>
-	~~[[4n80]] is~~ a ~~2 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4N80 OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024724564</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Shang, G J.]]		[[Category: Shang, G J.]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Lysozyme fold]]		[[Category: Lysozyme fold]]

OCA at 07:43, 23 April 2014

OCA — Wed, 23 Apr 2014 07:43:07 GMT

←Older revision		Revision as of 07:43, 23 April 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4n80\| PDB=4n80 \| SCENE= }}
		+	===Crystal structure of Tse3-Tsi3 complex===
		+	{{ABSTRACT_PUBMED_24724564}}

-	~~The entry~~ 4n80 is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4n80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N80 OCA].

-	~~Authors~~: Shang, G.J.	+	==Reference==
-		+	<ref group="xtra">PMID:024724564</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Toxin and anti~~-~~toxin~~ complex	+	[[Category: Shang, G J.]]
		+	[[Category: Hydrolase-hydrolase inhibitor complex]]
		+	[[Category: Lysozyme fold]]

OCA at 07:03, 6 November 2013

OCA — Wed, 06 Nov 2013 07:03:36 GMT

←Older revision		Revision as of 07:03, 6 November 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4n80 is ON HOLD	+	The entry 4n80 is ON HOLD until Paper Publication

	Authors: Shang, G.J.		Authors: Shang, G.J.

	Description: Toxin and anti-toxin complex		Description: Toxin and anti-toxin complex