4nbp - Revision history

OCA at 08:20, 11 January 2023

OCA — Wed, 11 Jan 2023 08:20:10 GMT

←Older revision		Revision as of 08:20, 11 January 2023
Line 1:		Line 1:

	==Crystal structure of the JCV large T-antigen origin binding domain==		==Crystal structure of the JCV large T-antigen origin binding domain==
-	<StructureSection load='4nbp' size='340' side='right' caption='[[4nbp]], [[Resolution\|resolution]] 1.31Å' scene=''>	+	<StructureSection load='4nbp' size='340' side='right'caption='[[4nbp]], [[Resolution\|resolution]] 1.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4nbp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human_polyomavirus_(type_jc) Human~~ polyomavirus ~~(type jc)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NBP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4NBP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4nbp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/JC_polyomavirus JC polyomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NBP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NBP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lif\|4lif]], [[4lmd\|4lmd]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nbp OCA], [https://pdbe.org/4nbp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nbp RCSB], [https://www.ebi.ac.uk/pdbsum/4nbp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nbp ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">large T antigen ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10632 Human polyomavirus (type JC)])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4nbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nbp OCA], [~~http~~://pdbe.org/4nbp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4nbp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4nbp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4nbp ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LT_POVJC LT_POVJC]] Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription (By similarity).	+	[https://www.uniprot.org/uniprot/LT_POVJC LT_POVJC] Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bohm, A~~]]	+	[[Category: JC polyomavirus]]
-	[[Category: ~~Bullock, P~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Meinke, G~~]]	+	[[Category: Bohm A]]
-	[[Category: ~~Binds dna~~]]	+	[[Category: Bullock P]]
-	[[Category: ~~Dna replication]]~~	+	[[Category: Meinke G]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Origin binding domain~~]]	+

OCA at 15:08, 4 August 2016

OCA — Thu, 04 Aug 2016 15:08:55 GMT

←Older revision		Revision as of 15:08, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the JCV large T-antigen origin binding domain==		==Crystal structure of the JCV large T-antigen origin binding domain==
	<StructureSection load='4nbp' size='340' side='right' caption='[[4nbp]], [[Resolution\|resolution]] 1.31Å' scene=''>		<StructureSection load='4nbp' size='340' side='right' caption='[[4nbp]], [[Resolution\|resolution]] 1.31Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lif\|4lif]], [[4lmd\|4lmd]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lif\|4lif]], [[4lmd\|4lmd]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">large T antigen ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10632 Human polyomavirus (type JC)])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">large T antigen ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10632 Human polyomavirus (type JC)])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nbp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nbp RCSB], [http://www.ebi.ac.uk/pdbsum/4nbp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nbp OCA], [http://pdbe.org/4nbp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nbp RCSB], [http://www.ebi.ac.uk/pdbsum/4nbp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nbp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4nbp" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 16:08, 24 December 2014

OCA — Wed, 24 Dec 2014 16:08:21 GMT

←Older revision		Revision as of 16:08, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4nbp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_polyomavirus_(type_jc) Human polyomavirus (type jc)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NBP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NBP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4nbp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_polyomavirus_(type_jc) Human polyomavirus (type jc)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NBP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NBP FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lif\|4lif]], [[4lmd\|4lmd]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lif\|4lif]], [[4lmd\|4lmd]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">large T antigen ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10632 Human polyomavirus (type JC)])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">large T antigen ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10632 Human polyomavirus (type JC)])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nbp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nbp RCSB], [http://www.ebi.ac.uk/pdbsum/4nbp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nbp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nbp RCSB], [http://www.ebi.ac.uk/pdbsum/4nbp PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LT_POVJC LT_POVJC]] Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	Insights into the initiation of JC virus DNA replication derived from the crystal structure of the T-antigen origin binding domain.,Meinke G, Phelan PJ, Kalekar R, Shin J, Archambault J, Bohm A, Bullock PA PLoS Pathog. 2014 Feb 20;10(2):e1003966. doi: 10.1371/journal.ppat.1003966., eCollection 2014 Feb. PMID:24586168<ref>PMID:24586168</ref>		Insights into the initiation of JC virus DNA replication derived from the crystal structure of the T-antigen origin binding domain.,Meinke G, Phelan PJ, Kalekar R, Shin J, Archambault J, Bohm A, Bullock PA PLoS Pathog. 2014 Feb 20;10(2):e1003966. doi: 10.1371/journal.ppat.1003966., eCollection 2014 Feb. PMID:24586168<ref>PMID:24586168</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Large T Antigen\|Large T Antigen]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bohm, A.]]	+	[[Category: Bohm, A]]
-	[[Category: Bullock, P.]]	+	[[Category: Bullock, P]]
-	[[Category: Meinke, G.]]	+	[[Category: Meinke, G]]
	[[Category: Binds dna]]		[[Category: Binds dna]]
	[[Category: Dna replication]]		[[Category: Dna replication]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Origin binding domain]]		[[Category: Origin binding domain]]

OCA at 12:24, 18 May 2014

OCA — Sun, 18 May 2014 12:24:21 GMT

←Older revision		Revision as of 12:24, 18 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4nbp\| PDB=4nbp \| SCENE= }}~~	+	==Crystal structure of the JCV large T-antigen origin binding domain==
-	===Crystal structure of the JCV large T-antigen origin binding domain===	+	<StructureSection load='4nbp' size='340' side='right' caption='[[4nbp]], [[Resolution\|resolution]] 1.31Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24586168}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4nbp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_polyomavirus_(type_jc) Human polyomavirus (type jc)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NBP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NBP FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lif\|4lif]], [[4lmd\|4lmd]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">large T antigen ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10632 Human polyomavirus (type JC)])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nbp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nbp RCSB], [http://www.ebi.ac.uk/pdbsum/4nbp PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	JC virus is a member of the Polyomavirus family of DNA tumor viruses and the causative agent of progressive multifocal leukoencephalopathy (PML). PML is a disease that occurs primarily in people who are immunocompromised and is usually fatal. As with other Polyomavirus family members, the replication of JC virus (JCV) DNA is dependent upon the virally encoded protein T-antigen. To further our understanding of JCV replication, we have determined the crystal structure of the origin-binding domain (OBD) of JCV T-antigen. This structure provides the first molecular understanding of JCV T-ag replication functions; for example, it suggests how the JCV T-ag OBD site-specifically binds to the major groove of GAGGC sequences in the origin. Furthermore, these studies suggest how the JCV OBDs interact during subsequent oligomerization events. We also report that the OBD contains a novel "pocket"; which sequesters the A1 & B2 loops of neighboring molecules. Mutagenesis of a residue in the pocket associated with the JCV T-ag OBD interfered with viral replication. Finally, we report that relative to the SV40 OBD, the surface of the JCV OBD contains one hemisphere that is highly conserved and one that is highly variable.

-	~~==Function==~~	+	Insights into the initiation of JC virus DNA replication derived from the crystal structure of the T-antigen origin binding domain.,Meinke G, Phelan PJ, Kalekar R, Shin J, Archambault J, Bohm A, Bullock PA PLoS Pathog. 2014 Feb 20;10(2):e1003966. doi: 10.1371/journal.ppat.1003966., eCollection 2014 Feb. PMID:24586168<ref>PMID:24586168</ref>
-	[[http://www.uniprot.org/uniprot/LT_POVJC LT_POVJC]] Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the ~~cell cycle and by autoregulating the synthesis~~ of ~~viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription,~~ replication~~, and repair of DNA. Participates in~~ the ~~modulation~~ of ~~cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces~~ the ~~disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1~~-~~regulated S-phase genes~~. ~~Inhibits host TP53 binding to DNA~~, ~~abrogating the ability of TP53 to stimulate gene expression~~. ~~Plays the role of a TFIID-associated factor~~ (~~TAF~~) ~~in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters~~. ~~Initiates viral DNA replication and unwinding via interactions with the viral origin of replication~~. ~~Binds two adjacent sites in the SV40 origin~~. ~~The replication fork movement is facilitated by Large T antigen helicase activity~~. ~~Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization~~. ~~Interferes with histone deacetylation mediated by HDAC1~~, ~~leading to activation of transcription (By similarity)~~.	+

-	~~==About this Structure==~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4nbp]] is~~ a ~~1 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4NBP OCA].~~	+	</div>
-		+	== References ==
-	==~~See Also==~~	+	<references/>
-	*[[Large T Antigen\|Large T Antigen]]	+	__TOC__
-		+	</StructureSection>
-	~~==Reference~~==	+
-	~~<ref group="xtra">PMID:024586168</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+
	[[Category: Bohm, A.]]		[[Category: Bohm, A.]]
	[[Category: Bullock, P.]]		[[Category: Bullock, P.]]

OCA at 07:08, 2 April 2014

OCA — Wed, 02 Apr 2014 07:08:49 GMT

←Older revision		Revision as of 07:08, 2 April 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4nbp\| PDB=4nbp \| SCENE= }}
		+	===Crystal structure of the JCV large T-antigen origin binding domain===
		+	{{ABSTRACT_PUBMED_24586168}}

-	~~The~~ entry ~~4nbp~~ is ~~ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/LT_POVJC LT_POVJC]] Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription (By similarity).

-	~~Authors~~: ~~Meinke, G~~.~~, Bohm, A~~.~~, Bullock, P~~.	+	==About this Structure==
		+	[[4nbp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NBP OCA].

-	~~Description~~: ~~Crystal structure of the JCV~~ Origin ~~Binding Domain~~	+	==See Also==
		+	*[[Large T Antigen\|Large T Antigen]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024586168</ref><references group="xtra"/><references/>
		+	[[Category: Bohm, A.]]
		+	[[Category: Bullock, P.]]
		+	[[Category: Meinke, G.]]
		+	[[Category: Binds dna]]
		+	[[Category: Dna replication]]
		+	[[Category: Hydrolase]]
		+	[[Category: Origin binding domain]]

OCA at 06:47, 4 December 2013

OCA — Wed, 04 Dec 2013 06:47:56 GMT

←Older revision		Revision as of 06:47, 4 December 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4nbp is ON HOLD	+	The entry 4nbp is ON HOLD until Paper Publication

	Authors: Meinke, G., Bohm, A., Bullock, P.		Authors: Meinke, G., Bohm, A., Bullock, P.

	Description: Crystal structure of the JCV Origin Binding Domain		Description: Crystal structure of the JCV Origin Binding Domain

OCA: Protected "4nbp" [edit=sysop:move=sysop]

OCA — Wed, 06 Nov 2013 07:07:45 GMT

Protected "4nbp" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:07, 6 November 2013

OCA: New page: '''Unreleased structure''' The entry 4nbp is ON HOLD Authors: Meinke, G., Bohm, A., Bullock, P. Description: Crystal structure of the JCV Origin Binding Domain

OCA — Wed, 06 Nov 2013 07:07:45 GMT

New page: '''Unreleased structure''' The entry 4nbp is ON HOLD Authors: Meinke, G., Bohm, A., Bullock, P. Description: Crystal structure of the JCV Origin Binding Domain

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'''Unreleased structure'''

The entry 4nbp is ON HOLD

Authors: Meinke, G., Bohm, A., Bullock, P.

Description: Crystal structure of the JCV Origin Binding Domain